Mouse Exo-Check Exosomes Antibody Arrays

SBI’s Mouse Exo-Check Exosomes Antibody Arrays take advantage of several exosome bona fide markers to ease the validation process, method development, and quality control of exosomes isolated by SEC, precipitation, or ultra-centrifugation.
  • Sample size: requires as little as 10µg of exosomal protein for detection (50 µg is optimal)
  • Convenient: 8 known antibodies for exosome bona fide markers
  • Flexibe: compatible with most exosome isolation methods, include the ExoQuick® family of reagents, SmartSEC, and ultracentrifugation
  • Complete: includes a background control (blank spot) and a positive control for HRP validation
  • Semi-quantitative: can be used to evaluate relative abundance of certain exosome markers from a given set of samples

Products

Catalog Number Description Size Price Quantity Add to Cart
EXORAY400A-4 Mouse Exo-Check Exosome Antibody Array 4 Arrays $570
- +
EXORAY400A-8 Mouse Exo-Check Exosome Antibody Array 8 Arrays $1016
- +

Overview

Streamline your mouse exosome detection with Exo-Check Arrays For the most efficient detection of exosomes, turn to our semi-quantitative Exo-Check Exosome Antibody Arrays. Each array has 12 pre-printed spots and features 8 antibodies for known exosome markers (Cd63, Cd81, Alix, Flot1, Icam1, EpCam, Anxa5 and Tsg101), a Gm130 cis-Golgi matrix marker (Gm130), a labeled positive control for HRP detection, and a blank spot as a background control. The kits come complete with a secondary detection mixture conjugated to HRP.

  • Sample size: requires as little as 10µg of exosomal protein for detection (50 µg is optimal)
  • Convenient: 8 known antibodies for exosome bona fide markers
  • Flexibe: compatible with most exosome isolation methods, include the ExoQuick® family of reagents, SmartSEC, and ultracentrifugation
  • Complete: includes a background control (blank spot) and a positive control for HRP validation
  • Semi-quantitative: can be used to evaluate relative abundance of certain exosome markers from a given set of samples

How It Works

A general reference to mouse Exo-Check Exosome Array

 width=
Spot Position *IDNOTESLINK TO EXOCARTA ENTRY FOR THE MOUSE PROTEINS
A1Cd63TetraspaninExoCarta_12512
A2EpcamEpithelial call adhesion molecule; often found in cancer-derived exosomesUniProt_Q99JW5
A3Anxa5Annexin A5ExoCarta_11747
A4Tsg101Tumor susceptibility gene 101ExoCarta_22088
A5BlankBackground controlN/A
A6Positive Control(+) control for HRP detectionN/A
B1Positive Control(+) control for HRP detectionN/A
B2Gm130Cis-golgi matrix proteinN/A
B3Flot1Flotillin-1ExoCarta_14251
B4Icam1Intercellular adhesion molecule 1ExoCarta_15894
B5AlixProgrammed cell death 6 interacting protein (Pdcd6ip)ExoCarta_18571
B6Cd81TetraspaninExoCarta_12520

Supporting Data

Figure 1. Serum- and cell culture-derived exosome analyzed with mouse Exo-Check Exosome Antibody Array

The array was exposed to 50 µg of exosome proteins isolated from wildtype mouse serum (A) via SmartSEC™ Single kit and RAW 264.7 mouse macrophage cells (B) using ExoQuick-TC®. The positive control band should provide strong signals to indicate that all the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signals, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal.

FAQs

Resources

Citations

Products

Catalog Number Description Size Price Quantity Add to Cart
EXORAY400A-4 Mouse Exo-Check Exosome Antibody Array 4 Arrays $570
- +
EXORAY400A-8 Mouse Exo-Check Exosome Antibody Array 8 Arrays $1016
- +

Overview

Streamline your mouse exosome detection with Exo-Check Arrays For the most efficient detection of exosomes, turn to our semi-quantitative Exo-Check Exosome Antibody Arrays. Each array has 12 pre-printed spots and features 8 antibodies for known exosome markers (Cd63, Cd81, Alix, Flot1, Icam1, EpCam, Anxa5 and Tsg101), a Gm130 cis-Golgi matrix marker (Gm130), a labeled positive control for HRP detection, and a blank spot as a background control. The kits come complete with a secondary detection mixture conjugated to HRP.

  • Sample size: requires as little as 10µg of exosomal protein for detection (50 µg is optimal)
  • Convenient: 8 known antibodies for exosome bona fide markers
  • Flexibe: compatible with most exosome isolation methods, include the ExoQuick® family of reagents, SmartSEC, and ultracentrifugation
  • Complete: includes a background control (blank spot) and a positive control for HRP validation
  • Semi-quantitative: can be used to evaluate relative abundance of certain exosome markers from a given set of samples

How It Works

A general reference to mouse Exo-Check Exosome Array

 width=
Spot Position *IDNOTESLINK TO EXOCARTA ENTRY FOR THE MOUSE PROTEINS
A1Cd63TetraspaninExoCarta_12512
A2EpcamEpithelial call adhesion molecule; often found in cancer-derived exosomesUniProt_Q99JW5
A3Anxa5Annexin A5ExoCarta_11747
A4Tsg101Tumor susceptibility gene 101ExoCarta_22088
A5BlankBackground controlN/A
A6Positive Control(+) control for HRP detectionN/A
B1Positive Control(+) control for HRP detectionN/A
B2Gm130Cis-golgi matrix proteinN/A
B3Flot1Flotillin-1ExoCarta_14251
B4Icam1Intercellular adhesion molecule 1ExoCarta_15894
B5AlixProgrammed cell death 6 interacting protein (Pdcd6ip)ExoCarta_18571
B6Cd81TetraspaninExoCarta_12520

Supporting Data

Figure 1. Serum- and cell culture-derived exosome analyzed with mouse Exo-Check Exosome Antibody Array

The array was exposed to 50 µg of exosome proteins isolated from wildtype mouse serum (A) via SmartSEC™ Single kit and RAW 264.7 mouse macrophage cells (B) using ExoQuick-TC®. The positive control band should provide strong signals to indicate that all the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signals, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal.

FAQs

Citations