PEG-it Virus Precipitation Solution

Easily concentrate lentivirus with an ultracentrifugation-free protocol—great for scaled-up virus production and also protects virus from freeze-thaw cycles

Description
Size
Catalog Number
Price
Quantity
Add to Cart

PEG-it Virus Precipitation Solution

Array ( [attributes] => Array ( [attribute_size] => 100 mL ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 295 [display_regular_price] => 295 [image] => Array ( [title] => purefect-gfp--sirna-data-crop1 [caption] => [url] => https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1.png [srcset] => https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1.png 345w, https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1-300x300.png 300w, https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1-100x100.png 100w [sizes] => (max-width: 345px) 100vw, 345px [full_src] => https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1.png [full_src_w] => 345 [full_src_h] => 345 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 345 [src_h] => 345 ) [image_id] => 20569 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $295 [sku] => LV810A-1 [variation_description] =>

PEG-it Virus Precipitation Solution

[variation_id] => 63918 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
100 mL
LV810A-1
$ 295

PEG-it Virus Precipitation Solution

Array ( [attributes] => Array ( [attribute_size] => 250 mL ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 643 [display_regular_price] => 643 [image] => Array ( [title] => purefect-gfp--sirna-data-crop1 [caption] => [url] => https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1.png [srcset] => https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1.png 345w, https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1-300x300.png 300w, https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1-100x100.png 100w [sizes] => (max-width: 345px) 100vw, 345px [full_src] => https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1.png [full_src_w] => 345 [full_src_h] => 345 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/lentiviral-production/purefect-gfp--sirna-data-crop1-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 345 [src_h] => 345 ) [image_id] => 20569 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $643 [sku] => LV825A-1 [variation_description] =>

PEG-it Virus Precipitation Solution

[variation_id] => 63919 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
250 mL
LV825A-1
$ 643
Contact Us Speak to a specialist
1-888-266-5066

Overview

Easily concentrate lentivirus particles for ultra-high titers

Used in over three-hundred citations, PEG-itTM Virus Concentration Reagent enables easy concentration of pseudoviral particles for achieving ultra-high titers. It’s great for concentrating pseudoviral particles even from large volumes of medium by removing the need for ultracentrifugation. Simply add PEG-it to the collected medium, incubate overnight at 4°C, and spin at 1500g for 30 minutes.

In addition, PEG-it acts as a cryopreservative for concentrated virus. Lentivirus concentrated with PEG-it lasts longer in the freezer and survives multiple freeze-thaw cycles with minimal loss of titer.

How It Works

PEG-it is an easy way to get higher viral titers by concentrating virus

Using PEG-it to concentrate virus particles is easy with little hands-on time

Supporting Data

PEG-it concentrates virus particles 10- to 100-fold

PEG-it concentrates virus particles 10- to 100-fold

Figure 1. PEG-it concentrates virus particles 10- to 100-fold. With PEG-it, virus particles can be concentrated up to 100-fold, leading to much higher infectious units per mL.

PEG-it is non-toxic and effective with stem cellsPEG-it is non-toxic and effective with stem cells

Figure 2. PEG-it is non-toxic and effective with Stem Cells. PEG-it-treated virus is non-toxic to stem cells and results in highly effective transductions. METHODS: H9 hES cells were transduced with pGreenZeo reporter constructs containing specific promoters for CMV, mOCT4 or mNANOG. Cells were cultured for eight weeks on Matrigel-coated plates with MEF conditioned medium containing 1 µg/mL Zeocin. The cells imaged here were split and grown on MEF feeder layers for four days. Data courtesy of Dr. Timothy Kamp and Chad H. Koonce, University of Wisconsin, Madison, Stem Cell and Regenerative Medicine Center.

PEG-it protects isolated virus particles from multiple freeze/thaw cyclesPEG-it protects isolated virus particles from multiple freeze/thaw cycles

Figure 3. PEG-it protects isolated virus particles from multiple freeze/thaw cycles. Lentivirus concentrated with PEG-it retains high titers even after four freeze-thaw cycles. HepG2 cells (top panel set) and HT1080 cells (bottom panel set) transduced with LV605VA-1 after 1 – 4 freeze-thaw cycles. Even after the fourth thaw, the LV605VA-1 virus particles show robust transduction efficiency.

Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective

Figure 4. Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective. The cells from Figure 3 were lysed and viral infectious units (IFUs) measured using the Global UltraRapid™ Lentiviral Titer Kit (Cat.# LV961A-1). No significant loss of IFUs due to repeated freeze/thaw cycles was found with virus particles that had been concentrated using PEG-it.


Citations

  • Szegő, ÉM, Gerhardt, E & Outeiro, TF. (2017) Sirtuin 2 enhances dopaminergic differentiation via the AKT/GSK-3β/β-catenin pathway. Neurobiol. Aging. 2017 Aug 1; 56:7-16. PM ID: 28478325
  • Zhan, P, et al. (2017) PRC1 contributes to tumorigenesis of lung adenocarcinoma in association with the Wnt/β-catenin signaling pathway. Mol. Cancer. 2017 Jun 24; 16(1):108. PM ID: 28646916
  • Emmett, MJ, et al. (2017) Histone deacetylase 3 prepares brown adipose tissue for acute thermogenic challenge. Nature. 2017 Jun 22; 546(7659):544-548. PM ID: 28614293
  • Greer, SU, et al. (2017) Linked read sequencing resolves complex genomic rearrangements in gastric cancer metastases. Genome Med. 2017 Jun 19; 9(1):57. PM ID: 28629429
  • Paiva, I, et al. (2017) Sodium butyrate rescues dopaminergic cells from alpha-synuclein-induced transcriptional deregulation and DNA damage. Hum. Mol. Genet.. 2017 Jun 15; 26(12):2231-2246. PM ID: 28369321
  • Flodby, P, et al. (2017) Cell-specific expression of aquaporin-5 (Aqp5) in alveolar epithelium is directed by GATA6/Sp1 via histone acetylation. Sci Rep. 2017 Jun 14; 7(1):3473. PM ID: 28615712
  • Liang, X, et al. (2017) Histone Chaperone ASF1A Predicts Poor Outcomes for Patients With Gastrointestinal Cancer and Drives Cancer Progression by Stimulating Transcription of β-Catenin Target Genes. EBioMedicine. 2017 Jun 8;. PM ID: 28625518
  • Nakagawa, R. (2017) Germinal Center Formation with Retrovirally Transduced B Cells for Determining the Role of Specific Molecules In Vivo. Methods Mol. Biol.. 2017 Jun 7; 1623:147-158. PM ID: 28589355
  • De Luca, L, et al. (2017) Knockdown of miR-128a induces Lin28a expression and reverts myeloid differentiation blockage in acute myeloid leukemia. Cell Death Dis. 2017 Jun 1; 8(6):e2849. PM ID: 28569789
  • Eisfeld, AK, et al. (2017) Mutations in the CCND1 and CCND2 genes are frequent events in adult patients with t(8;21)(q22;q22) acute myeloid leukemia.. Leukemia. 2017 Jun 1; 31(6):1278-1285. PM ID: 27843138
  • Wang, G, et al. (2017) Role of OSGIN1 in mediating smoking-induced autophagy in the human airway epithelium. Autophagy. 2017 May 26;:1-16. PM ID: 28548877
  • Chen, H, et al. (2017) Mesenchymal Stem Cells Expressing eNOS and a Cav1 Mutant Inhibit Vascular Smooth Muscle Cell Proliferation in a Rat Model of Pulmonary Hypertension. Heart Lung Circ. 2017 May 1; 26(5):509-518. PM ID: 27771236
  • Marsboom, G, et al. (2017) Aberrant caveolin-1–mediated Smad signaling and proliferation identified by analysis of adenine 474 deletion mutation (c.474delA) in patient fibroblasts: a new perspective on the mechanism of pulmonary hypertension. Molecular biology of the Cell. 2017 May 1; 28(9):1177-1185. Link: Molecular biology of the Cell
  • Qian, D, et al. (2017) Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats. Sci Rep. 2017 Apr 3; 7(1):581. PM ID: 28373667
  • Wakeland, AK, et al. (2017) Hypoxia Directs Human Extravillous Trophoblast Differentiation in a Hypoxia-Inducible Factor-Dependent Manner. Am. J. Pathol.. 2017 Apr 1; 187(4):767-780. PM ID: 28167044
  • Chuang, W, et al. (2017) Partial Reprogramming of Pluripotent Stem Cell-Derived Cardiomyocytes into Neurons. Sci Rep. 2017 Mar 22; 7:44840. PM ID: 28327614
  • Decker, JT, et al. (2017) Systems analysis of dynamic transcription factor activity identifies targets for treatment in Olaparib resistant cancer cells. Biotechnol. Bioeng.. 2017 Mar 21;. PM ID: 28322442
  • Marsboom, G, et al. (2017) Aberrant Caveolin-1-Mediated Smad Signaling and Proliferation Identified by Analysis of Adenine 474 Deletion Mutation (c.474delA) in Patient Fibroblasts: A New Perspective in the Mechanism of Pulmonary Hypertension. Mol. Biol. Cell. 2017 Mar 15;. PM ID: 28298490
  • Chan, CY, Cheuk, BL & Cheng, SW. (2017) Abdominal Aortic Aneurysm-Associated MicroRNA-516a-5p Regulates Expressions of Methylenetetrahydrofolate Reductase, Matrix Metalloproteinase-2, and Tissue Inhibitor of Matrix Metalloproteinase-1 in Human Abdominal Aortic Vascular Smooth Muscle Cells. Ann Vasc Surg. 2017 Mar 10;. PM ID: 28288890
  • Wan, L, et al. (2017) ENL links histone acetylation to oncogenic gene expression in acute myeloid leukaemia. Nature. 2017 Mar 9; 543(7644):265-269. PM ID: 28241141