PEG-it Virus Precipitation Solution

Easily concentrate lentivirus with an ultracentrifugation-free protocol—great for scaled-up virus production and also protects virus from freeze-thaw cycles

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PEG-it Virus Precipitation Solution

100 mL
$ 299

PEG-it Virus Precipitation Solution

250 mL
$ 653
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Easily concentrate lentivirus particles for ultra-high titers

Used in over three-hundred citations, PEG-it™ Virus Concentration Reagent enables easy concentration of pseudoviral particles for achieving ultra-high titers. It’s great for concentrating pseudoviral particles even from large volumes of medium by removing the need for ultracentrifugation. Simply add PEG-it to the collected medium, incubate overnight at 4°C, and spin at 1500g for 30 minutes.

In addition, PEG-it acts as a cryopreservative for concentrated virus. Lentivirus concentrated with PEG-it lasts longer in the freezer and survives multiple freeze-thaw cycles with minimal loss of titer.

How It Works

PEG-it is an easy way to get higher viral titers by concentrating virus

Using PEG-it to concentrate virus particles is easy with little hands-on time

Supporting Data

PEG-it concentrates virus particles 10- to 100-fold

PEG-it concentrates virus particles 10- to 100-fold

Figure 1. PEG-it concentrates virus particles 10- to 100-fold. With PEG-it, virus particles can be concentrated up to 100-fold, leading to much higher infectious units per mL.

PEG-it is non-toxic and effective with stem cellsPEG-it is non-toxic and effective with stem cells

Figure 2. PEG-it is non-toxic and effective with Stem Cells. PEG-it-treated virus is non-toxic to stem cells and results in highly effective transductions. METHODS: H9 hES cells were transduced with pGreenZeo reporter constructs containing specific promoters for CMV, mOCT4 or mNANOG. Cells were cultured for eight weeks on Matrigel-coated plates with MEF conditioned medium containing 1 µg/mL Zeocin. The cells imaged here were split and grown on MEF feeder layers for four days. Data courtesy of Dr. Timothy Kamp and Chad H. Koonce, University of Wisconsin, Madison, Stem Cell and Regenerative Medicine Center.

PEG-it protects isolated virus particles from multiple freeze/thaw cyclesPEG-it protects isolated virus particles from multiple freeze/thaw cycles

Figure 3. PEG-it protects isolated virus particles from multiple freeze/thaw cycles. Lentivirus concentrated with PEG-it retains high titers even after four freeze-thaw cycles. HepG2 cells (top panel set) and HT1080 cells (bottom panel set) transduced with LV605VA-1 after 1 – 4 freeze-thaw cycles. Even after the fourth thaw, the LV605VA-1 virus particles show robust transduction efficiency.

Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective

Figure 4. Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective. The cells from Figure 3 were lysed and viral infectious units (IFUs) measured using the Global UltraRapid™ Lentiviral Titer Kit (Cat.# LV961A-1). No significant loss of IFUs due to repeated freeze/thaw cycles was found with virus particles that had been concentrated using PEG-it.


  • Lichinchi, G & Rana, TM. (2018) Profiling of N6-Methyladenosine in Zika Virus RNA and Host Cellular mRNA. Methods Mol. Biol.. 2018 Dec 13; 1870:209-218. PM ID: 30539558
  • Jeon, YJ, et al. (2018) miRNA-mediated TUSC3 deficiency enhances UPR and ERAD to promote metastatic potential of NSCLC. Nat Commun. 2018 Nov 30; 9(1):5110. PM ID: 30504895
  • Fernandes, LN, et al. (2018) A Novel Highly Divergent Strain of Cell Fusing Agent Virus (CFAV) in Mosquitoes from the Brazilian Amazon Region. Viruses. 2018 Nov 24; 10(12). PM ID: 30477235
  • Luchs, A, et al. (2018) Spread of the emerging equine-like G3P[8] DS-1-like genetic backbone rotavirus strain in Brazil and identification of potential genetic variants. J. Gen. Virol.. 2018 Nov 20;. PM ID: 30457517
  • Decaesteker, B, et al. (2018) TBX2 is a neuroblastoma core regulatory circuitry component enhancing MYCN/FOXM1 reactivation of DREAM targets. Nat Commun. 2018 Nov 19; 9(1):4866. PM ID: 30451831
  • Nam, WH & Choi, YP. (2018) In vitro generation of tau aggregates conformationally distinct from parent tau seeds of Alzheimer’s brain. Prion. 2018 Nov 13;:1-12. PM ID: 30422056
  • Heo, H, et al. (2018) Improved dynamic monitoring of transcriptional activity during longitudinal analysis in the mouse brain. Biol Open. 2018 Oct 19;. PM ID: 30341106
  • Park, J, et al. (2018) Reducing inflammation through delivery of lentivirus encoding for anti-inflammatory cytokines attenuates neuropathic pain after spinal cord injury. J Control Release. 2018 Oct 6; 290:88-101. PM ID: 30296461
  • Hisano, Y, et al. (2018) Heterotypic inter-GPCR β-arrestin coupling regulates lymphatic endothelial junctional architecture in murine lymph nodes. bioRxiv. 2018 Oct 5;. Link: bioRxiv
  • da Costa, AC, et al. (2018) Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus. Viruses. 2018 Sep 24; 10(10). PM ID: 30249971
  • Peiris, H, et al. (2018) Discovering human diabetes-risk gene function with genetics and physiological assays. Nat Commun. 2018 Sep 21; 9(1):3855. PM ID: 30242153
  • Decker, JT, et al. (2018) Design of Large-Scale Reporter Construct Arrays for Dynamic, Live Cell Systems Biology. ACS Synth Biol. 2018 Sep 21; 7(9):2063-2073. PM ID: 30189139
  • Smith, DR, et al. (2018) Combinatorial lentiviral gene delivery of pro-oligodendrogenic factors to improve myelination of regenerating axons after spinal cord injury. Biotechnol. Bioeng.. 2018 Sep 19;. PM ID: 30229864
  • Gao, X, et al. (2018) Functional silencing of HSD17B2 in prostate cancer promotes disease progression. Clin. Cancer Res.. 2018 Sep 18;. PM ID: 30228209
  • Murakami, T, et al. (2018) Novel Human NK Cell Line Carrying CAR Targeting EGFRvIII Induces Antitumor Effects in Glioblastoma Cells. Anticancer Res.. 2018 Sep 1; 38(9):5049-5056. PM ID: 30194149
  • Kohl, C & Kurth, A. (2018) Tissue-Based Universal Virus Detection (TUViD-VM) Protocol for Viral Metagenomics. Methods Mol. Biol.. 2018 Aug 22; 1838:15-23. PM ID: 30128986
  • da Costa, AC, et al. (2018) Near full length genome of a recombinant (E/D) cosavirus strain from a rural area in the central region of Brazil. Sci Rep. 2018 Aug 17; 8(1):12304. PM ID: 30120342
  • Sun, J, et al. (2018) SIRT1 Activation Disrupts Maintenance of Myelodysplastic Syndrome Stem and Progenitor Cells by Restoring TET2 Function. Cell Stem Cell. 2018 Aug 15;. PM ID: 30146412
  • Wang, Y, et al. (2018) Upregulation of EID3 sensitizes breast cancer cells to ionizing radiation-induced cellular senescence. Biomed. Pharmacother.. 2018 Aug 13; 107:606-614. PM ID: 30114644
  • Brachtendorf, S, et al. (2018) Chemosensitivity of human colon cancer cells is influenced by a p53-dependent enhancement of ceramide synthase 5 and induction of autophagy. Biochim. Biophys. Acta. 2018 Jul 27;. PM ID: 30059758