PEG-it Virus Precipitation Solution

Easily concentrate lentivirus with an ultracentrifugation-free protocol—great for scaled-up virus production and also protects virus from freeze-thaw cycles

Products

Catalog Number Description Size Price Quantity Add to Cart
LV810A-1 PEG-it Virus Precipitation Solution 100 mL $319
- +
LV825A-1 PEG-it Virus Precipitation Solution 250 mL $697
- +

Overview

Overview

Easily concentrate lentivirus particles for ultra-high titers

Used in over three-hundred citations, PEG-it™ Virus Concentration Reagent enables easy concentration of pseudoviral particles for achieving ultra-high titers. It’s great for concentrating pseudoviral particles even from large volumes of medium by removing the need for ultracentrifugation. Simply add PEG-it to the collected medium, incubate overnight at 4°C, and spin at 1500g for 30 minutes.

In addition, PEG-it acts as a cryopreservative for concentrated virus. Lentivirus concentrated with PEG-it lasts longer in the freezer and survives multiple freeze-thaw cycles with minimal loss of titer.

How It Works

How It Works

PEG-it is an easy way to get higher viral titers by concentrating virus

Using PEG-it to concentrate virus particles is easy with little hands-on time

Supporting Data

Supporting Data

PEG-it concentrates virus particles 10- to 100-fold

PEG-it concentrates virus particles 10- to 100-fold

Figure 1. PEG-it concentrates virus particles 10- to 100-fold. With PEG-it, virus particles can be concentrated up to 100-fold, leading to much higher infectious units per mL.

PEG-it is non-toxic and effective with stem cellsPEG-it is non-toxic and effective with stem cells

Figure 2. PEG-it is non-toxic and effective with Stem Cells. PEG-it-treated virus is non-toxic to stem cells and results in highly effective transductions. METHODS: H9 hES cells were transduced with pGreenZeo reporter constructs containing specific promoters for CMV, mOCT4 or mNANOG. Cells were cultured for eight weeks on Matrigel-coated plates with MEF conditioned medium containing 1 µg/mL Zeocin. The cells imaged here were split and grown on MEF feeder layers for four days. Data courtesy of Dr. Timothy Kamp and Chad H. Koonce, University of Wisconsin, Madison, Stem Cell and Regenerative Medicine Center.

PEG-it protects isolated virus particles from multiple freeze/thaw cyclesPEG-it protects isolated virus particles from multiple freeze/thaw cycles

Figure 3. PEG-it protects isolated virus particles from multiple freeze/thaw cycles. Lentivirus concentrated with PEG-it retains high titers even after four freeze-thaw cycles. HepG2 cells (top panel set) and HT1080 cells (bottom panel set) transduced with LV605VA-1 after 1 – 4 freeze-thaw cycles. Even after the fourth thaw, the LV605VA-1 virus particles show robust transduction efficiency.

Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective

Figure 4. Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective. The cells from Figure 3 were lysed and viral infectious units (IFUs) measured using the Global UltraRapid™ Lentiviral Titer Kit (Cat.# LV961A-1). No significant loss of IFUs due to repeated freeze/thaw cycles was found with virus particles that had been concentrated using PEG-it.

Resources

Citations

  • Banskota, S, et al. (2022) Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins. Cell. 1970 Jan 1; 185(2):250-265.e16. PM ID: 35021064
  • Wang, F, et al. (2022) Single-cell architecture and functional requirement of alternative splicing during hematopoietic stem cell formation. Science advances. 1970 Jan 1; 8(1):eabg5369. PM ID: 34995116
  • Wang, B, et al. (2022) Pharmacological CDK4/6 inhibition reveals a p53-dependent senescent state with restricted toxicity. The EMBO journal. 1970 Jan 1;:e108946. PM ID: 34985783
  • Li, Y, et al. (2022) Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1β to downregulate PPARα and FOXO3. Cell reports. 1970 Jan 1; 38(4):110284. PM ID: 35081341
  • Beaudoin-Bussières, G, et al. (2022) A Fc-enhanced NTD-binding non-neutralizing antibody delays virus spread and synergizes with a nAb to protect mice from lethal SARS-CoV-2 infection. Cell Reports. 1970 Jan 1;:110368. Link: Cell Reports
  • Shimada, E, et al. (2022) Methylation-mediated silencing of protein kinase C zeta induces apoptosis avoidance through ATM/CHK2 inactivation in dedifferentiated chondrosarcoma. British journal of cancer. 1970 Jan 1;. PM ID: 35017658
  • Raabe, FJ, et al. (2022) Expression of Lineage Transcription Factors Identifies Differences in Transition States of Induced Human Oligodendrocyte Differentiation. Cells. 1970 Jan 1; 11(2). PM ID: 35053357
  • Lee, SS, et al. (2022) Development of SARS-CoV-2 packaged RNA reference material for nucleic acid testing. Analytical and bioanalytical chemistry. 1970 Jan 1; 414(5):1773-1785. PM ID: 34958396
  • Yan, Z, et al. (2022) Zinc finger protein 384 enhances colorectal cancer metastasis by upregulating MMP2. Oncology reports. 1970 Jan 1; 47(3). PM ID: 35029289
  • Zhou, Z, et al. (2022) Sirtuin 1 Protects the Mitochondria in Hepatocellular Carcinoma Cells via Suppressing Hypoxia-induced Factor-1 Alpha Expression. Research Square. 1970 Jan 1;. Link: Research Square
  • Gossner, A, Raper, A & Hassan, MA. (2022) Cell-based screen identifies human type I interferon-stimulated regulators of Toxoplasma gondii Infection. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Kim, I, et al. (2022) Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells. Science advances. 1970 Jan 1; 8(14):eabj4928. PM ID: 35385316
  • Wang, J, et al. (2022) PAT2 regulates vATPase assembly and lysosomal acidification in brown adipocytes. Molecular Metabolism. 1970 Jan 1;:101508. Link: Molecular Metabolism
  • Dai, T, et al. (2022) Hypoxic Tumor Microenvironment Induces Metabolic Enzyme PFKFB4 Acquiring Invasive Cancer Phenotype to Drive Metastasis. SSRN Electronic Journal. 1970 Jan 1;. Link: SSRN Electronic Journal
  • Kim, YJ, et al. (2022) Gene-specific nonsense-mediated mRNA decay targeting for cystic fibrosis therapy. Nature communications. 1970 Jan 1; 13(1):2978. PM ID: 35624092
  • Yuge, S, et al. (2022) Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins. Nature communications. 1970 Jan 1; 13(1):2594. PM ID: 35551172
  • Papes, F, et al. (2022) Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content. Nature communications. 1970 Jan 1; 13(1):2387. PM ID: 35501322
  • Jacquemin, G, et al. (2022) Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme. eLife. 1970 Jan 1; 11. PM ID: 35543624
  • Wei, Q, et al. (2022) Dual-Role of Cholesterol-25-Hydroxylase in Regulating Hepatitis B Virus Infection and Replication. mBio. 1970 Jan 1;:e0067722. PM ID: 35587189
  • Zhuang, X & Long, EO. (2022) NK Cells Equipped With a Chimeric Antigen Receptor That Overcomes Inhibition by HLA Class I for Adoptive Transfer of CAR-NK Cells. Frontiers in immunology. 1970 Jan 1; 13:840844. PM ID: 35585985

Products

Catalog Number Description Size Price Quantity Add to Cart
LV810A-1 PEG-it Virus Precipitation Solution 100 mL $319
- +
LV825A-1 PEG-it Virus Precipitation Solution 250 mL $697
- +

Overview

Overview

Easily concentrate lentivirus particles for ultra-high titers

Used in over three-hundred citations, PEG-it™ Virus Concentration Reagent enables easy concentration of pseudoviral particles for achieving ultra-high titers. It’s great for concentrating pseudoviral particles even from large volumes of medium by removing the need for ultracentrifugation. Simply add PEG-it to the collected medium, incubate overnight at 4°C, and spin at 1500g for 30 minutes.

In addition, PEG-it acts as a cryopreservative for concentrated virus. Lentivirus concentrated with PEG-it lasts longer in the freezer and survives multiple freeze-thaw cycles with minimal loss of titer.

How It Works

How It Works

PEG-it is an easy way to get higher viral titers by concentrating virus

Using PEG-it to concentrate virus particles is easy with little hands-on time

Supporting Data

Supporting Data

PEG-it concentrates virus particles 10- to 100-fold

PEG-it concentrates virus particles 10- to 100-fold

Figure 1. PEG-it concentrates virus particles 10- to 100-fold. With PEG-it, virus particles can be concentrated up to 100-fold, leading to much higher infectious units per mL.

PEG-it is non-toxic and effective with stem cellsPEG-it is non-toxic and effective with stem cells

Figure 2. PEG-it is non-toxic and effective with Stem Cells. PEG-it-treated virus is non-toxic to stem cells and results in highly effective transductions. METHODS: H9 hES cells were transduced with pGreenZeo reporter constructs containing specific promoters for CMV, mOCT4 or mNANOG. Cells were cultured for eight weeks on Matrigel-coated plates with MEF conditioned medium containing 1 µg/mL Zeocin. The cells imaged here were split and grown on MEF feeder layers for four days. Data courtesy of Dr. Timothy Kamp and Chad H. Koonce, University of Wisconsin, Madison, Stem Cell and Regenerative Medicine Center.

PEG-it protects isolated virus particles from multiple freeze/thaw cyclesPEG-it protects isolated virus particles from multiple freeze/thaw cycles

Figure 3. PEG-it protects isolated virus particles from multiple freeze/thaw cycles. Lentivirus concentrated with PEG-it retains high titers even after four freeze-thaw cycles. HepG2 cells (top panel set) and HT1080 cells (bottom panel set) transduced with LV605VA-1 after 1 – 4 freeze-thaw cycles. Even after the fourth thaw, the LV605VA-1 virus particles show robust transduction efficiency.

Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective

Figure 4. Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective. The cells from Figure 3 were lysed and viral infectious units (IFUs) measured using the Global UltraRapid™ Lentiviral Titer Kit (Cat.# LV961A-1). No significant loss of IFUs due to repeated freeze/thaw cycles was found with virus particles that had been concentrated using PEG-it.

Citations

  • Banskota, S, et al. (2022) Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins. Cell. 1970 Jan 1; 185(2):250-265.e16. PM ID: 35021064
  • Wang, F, et al. (2022) Single-cell architecture and functional requirement of alternative splicing during hematopoietic stem cell formation. Science advances. 1970 Jan 1; 8(1):eabg5369. PM ID: 34995116
  • Wang, B, et al. (2022) Pharmacological CDK4/6 inhibition reveals a p53-dependent senescent state with restricted toxicity. The EMBO journal. 1970 Jan 1;:e108946. PM ID: 34985783
  • Li, Y, et al. (2022) Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1β to downregulate PPARα and FOXO3. Cell reports. 1970 Jan 1; 38(4):110284. PM ID: 35081341
  • Beaudoin-Bussières, G, et al. (2022) A Fc-enhanced NTD-binding non-neutralizing antibody delays virus spread and synergizes with a nAb to protect mice from lethal SARS-CoV-2 infection. Cell Reports. 1970 Jan 1;:110368. Link: Cell Reports
  • Shimada, E, et al. (2022) Methylation-mediated silencing of protein kinase C zeta induces apoptosis avoidance through ATM/CHK2 inactivation in dedifferentiated chondrosarcoma. British journal of cancer. 1970 Jan 1;. PM ID: 35017658
  • Raabe, FJ, et al. (2022) Expression of Lineage Transcription Factors Identifies Differences in Transition States of Induced Human Oligodendrocyte Differentiation. Cells. 1970 Jan 1; 11(2). PM ID: 35053357
  • Lee, SS, et al. (2022) Development of SARS-CoV-2 packaged RNA reference material for nucleic acid testing. Analytical and bioanalytical chemistry. 1970 Jan 1; 414(5):1773-1785. PM ID: 34958396
  • Yan, Z, et al. (2022) Zinc finger protein 384 enhances colorectal cancer metastasis by upregulating MMP2. Oncology reports. 1970 Jan 1; 47(3). PM ID: 35029289
  • Zhou, Z, et al. (2022) Sirtuin 1 Protects the Mitochondria in Hepatocellular Carcinoma Cells via Suppressing Hypoxia-induced Factor-1 Alpha Expression. Research Square. 1970 Jan 1;. Link: Research Square
  • Gossner, A, Raper, A & Hassan, MA. (2022) Cell-based screen identifies human type I interferon-stimulated regulators of Toxoplasma gondii Infection. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Kim, I, et al. (2022) Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells. Science advances. 1970 Jan 1; 8(14):eabj4928. PM ID: 35385316
  • Wang, J, et al. (2022) PAT2 regulates vATPase assembly and lysosomal acidification in brown adipocytes. Molecular Metabolism. 1970 Jan 1;:101508. Link: Molecular Metabolism
  • Dai, T, et al. (2022) Hypoxic Tumor Microenvironment Induces Metabolic Enzyme PFKFB4 Acquiring Invasive Cancer Phenotype to Drive Metastasis. SSRN Electronic Journal. 1970 Jan 1;. Link: SSRN Electronic Journal
  • Kim, YJ, et al. (2022) Gene-specific nonsense-mediated mRNA decay targeting for cystic fibrosis therapy. Nature communications. 1970 Jan 1; 13(1):2978. PM ID: 35624092
  • Yuge, S, et al. (2022) Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins. Nature communications. 1970 Jan 1; 13(1):2594. PM ID: 35551172
  • Papes, F, et al. (2022) Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content. Nature communications. 1970 Jan 1; 13(1):2387. PM ID: 35501322
  • Jacquemin, G, et al. (2022) Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme. eLife. 1970 Jan 1; 11. PM ID: 35543624
  • Wei, Q, et al. (2022) Dual-Role of Cholesterol-25-Hydroxylase in Regulating Hepatitis B Virus Infection and Replication. mBio. 1970 Jan 1;:e0067722. PM ID: 35587189
  • Zhuang, X & Long, EO. (2022) NK Cells Equipped With a Chimeric Antigen Receptor That Overcomes Inhibition by HLA Class I for Adoptive Transfer of CAR-NK Cells. Frontiers in immunology. 1970 Jan 1; 13:840844. PM ID: 35585985