PureFectionTM Transfection Reagent

Deliver more nucleic acids—plasmids, siRNAs, etc.—to cells than the leading lipid-based transfection reagent for effective and reproducible transfection

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PureFection Transfection Reagent

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PureFection Transfection Reagent

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1 mL
LV750A-1
$ 273

PureFection Transfection Reagent

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PureFection Transfection Reagent

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5 mL
LV750A-5
$ 1158
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Overview

Increase your transfection efficiencies

With SBI’s PureFectionTM Transfection Reagent, you can deliver more nucleic acid—plasmids, siRNAs, etc.—than the leading lipid-based transfection reagent for effective, efficient, and reproducible transfections.

The easy-to-use protocol consists of a rapid, one-step, 15-minute incubation with the plasmid, small RNA, or other nucleic acid you’d like to transfect. Once the incubation is done, simply add directly to target cells—no media changes are required as PureFection works in the presence of antibiotics and serum.

The fast PureFection protocol makes it well-suited for high-throughput transfection projects.

  • Highly effective transfection technology—works with most cell types
  • Cost-effective alternative to lipid-based products
  • Nanoparticle-based gene delivery with low toxicity
  • Rapid 15-minute protocol makes PureFection ideal for high-throughput transfections
  • Works with both Plasmid DNA and siRNAs

How It Works

A fast and effective method for increasing transfection efficiencies

The PureFection Transfection Reagent uses a fast, 15-minute protocol

Supporting Data

PureFection delivers higher transfection efficiencies than the leading lipid-based reagent

PureFection delivers higher transfection efficiencies than the leading lipid-based reagentPureFection delivers higher transfection efficiencies than the leading lipid-based reagent

Citations

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  • Huang, CY, et al. (2017) HSF1 phosphorylation by ERK/GSK3 suppresses RNF126 to sustain IGF-IIR expression for hypertension-induced cardiomyocyte hypertrophy. J. Cell. Physiol.. 2017 Apr 6;. PM ID: 28383811
  • Chen, YP, et al. (2017) Short-term Hypoxia Reverses Ox-LDL-induced CD36 and GLUT4 Switching Metabolic Pathways in H9c2 Cardiomyoblast Cells. J. Cell. Biochem.. 2017 Apr 4;. PM ID: 28374891
  • Cheng, SY, et al. (2017) Lactate dehydrogenase downregulation mediates the inhibitory effect of diallyl trisulfide on proliferation, metastasis, and invasion in triple-negative breast cancer. Environ. Toxicol.. 2017 Apr 1; 32(4):1390-1398. PM ID: 27566995
  • Chang, YM, et al. (2017) Alpinia oxyphylla Miq. fruit extract activates IGFR-PI3K/Akt signaling to induce Schwann cell proliferation and sciatic nerve regeneration. BMC Complement Altern Med. 2017 Mar 31; 17(1):184. PM ID: 28359314
  • Huang, CY, et al. (2017) Mitochondrial ROS-induced ERK1/2 activation and HSF2-mediated AT1 R upregulation are required for doxorubicin-induced cardiotoxicity.. J. Cell. Physiol.. 2017 Mar 14;. PM ID: 28295305
  • Chen, Q, et al. (2016) FGF-2 Transcriptionally Down-Regulates the Expression of BNIP3L via PI3K/Akt/FoxO3a Signaling and Inhibits Necrosis and Mitochondrial Dysfunction Induced by High Concentrations of Hydrogen Peroxide in H9c2 Cells. Cell. Physiol. Biochem.. 2016 Dec 22; 40(6):1678-1691. PM ID: 28006775
  • Wu, H, et al. (2016) MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a and Regulating Renal Tubular Cell Pyroptosis. Cell. Physiol. Biochem.. 2016 Dec 22; 40(6):1692-1705. PM ID: 28006785
  • Fu, CY, et al. (2016) ZAKβ antagonizes and ameliorates the cardiac hypertrophic and apoptotic effects induced by ZAKα. Cell Biochem. Funct.. 2016 Dec 1; 34(8):606-612. PM ID: 27859413
  • Hsu, HH, et al. (2016) Taiwanin E inhibits cell migration in human LoVo colon cancer cells by suppressing MMP-2/9 expression via p38 MAPK pathway. Environ. Toxicol.. 2016 Nov 3;. PM ID: 27807932
  • Huang, CY, et al. (2016) Doxorubicin attenuates CHIP-guarded HSF1 nuclear translocation and protein stability to trigger IGF-IIR-dependent cardiomyocyte death. Cell Death Dis. 2016 Nov 3; 7(11):e2455. PM ID: 27809308
  • Dotimas, JR, et al. (2016) Diabetes regulates fructose absorption through thioredoxin-interacting protein. Elife. 2016 Oct 11; 5. PM ID: 27725089
  • Chatterjee, B, et al. (2016) p38α MAPK disables KMT1A-mediated repression of myogenic differentiation program. Skelet Muscle. 2016 Aug 23; 6:28. PM ID: 27551368
  • Feng, CC, et al. (2016) Hypoxia suppresses myocardial survival pathway through HIF-1α-IGFBP-3-dependent signaling and enhances cardiomyocyte autophagic and apoptotic effects mainly via FoxO3a-induced BNIP3 expression. Growth Factors. 2016 Aug 1; 34(3-4):73-86. PM ID: 27366871
  • Yeh, Y, et al. (2016) Hypoxia Augments Increased HIF-1 [alpha] and Reduced Survival Protein p-Akt in Gelsolin (GSN)-Dependent Cardiomyoblast Cell Apoptosis. Cell Biochem Biophys. 2016 May 18; 74:221–228. Link: Cell Biochem Biophys
  • Gjymishka, A, et al. (2016) miR-133b Regulation of Connective Tissue Growth Factor: A Novel Mechanism in Liver Pathology. Am. J. Pathol.. 2016 May 1; 186(5):1092-102. PM ID: 26945106
  • Chen, YP, et al. (2016) Palmitic acid interferes with energy metabolism balance by adversely switching the SIRT1-CD36-fatty acid pathway to the PKC zeta-GLUT4-glucose pathway in cardiomyoblasts. J. Nutr. Biochem.. 2016 May 1; 31:137-49. PM ID: 27133433
  • Elias, A, et al. (2016) Cancer-specific binary expression system activated in mice by bacteriophage HK022 Integrase. Sci Rep. 2016 Apr 27; 6:24971. PM ID: 27117628
  • Chang, JC, et al. (2016) Allogeneic/xenogeneic transplantation of peptide-labeled mitochondria in Parkinson’s disease: restoration of mitochondria functions and attenuation of 6-hydroxydopamine-induced neurotoxicity. Transl Res. 2016 Apr 1; 170:40-56.e1-3. PM ID: 26730494