pGreenFire Control Pack

Run your pGreenFire projects with confidence with all of the positive and negative pGreenFire1 controls bundled together

Description
Size
Catalog Number
Price
Quantity
Add to Cart

pGreenFire Control Pack- pGreenFire negative and positive control plasmids, only available with purchase of other pGF reporters

Array ( [attributes] => Array ( [attribute_size] => 10 µg Each ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 334 [display_regular_price] => 334 [image] => Array ( [title] => pgreenfire-control-pack-main-image-01 [caption] => [url] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire-control-pack-main-image-01.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire-control-pack-main-image-01-600x600.png [srcset] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire-control-pack-main-image-01-600x600.png 600w, https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire-control-pack-main-image-01-300x300.png 300w, https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire-control-pack-main-image-01-100x100.png 100w [sizes] => (max-width: 600px) 100vw, 600px [full_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire-control-pack-main-image-01.png [full_src_w] => 2500 [full_src_h] => 2500 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire-control-pack-main-image-01-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire-control-pack-main-image-01-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 600 [src_h] => 600 ) [image_id] => 20977 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $334 [sku] => TR000PA-1 [variation_description] =>

pGreenFire Control Pack- pGreenFire negative and positive control plasmids, only available with purchase of other pGF reporters

[variation_id] => 65677 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
10 µg Each
TR000PA-1
$ 334
Contact Us Speak to a specialist
1-888-266-5066

Overview

Supporting your studies with ready-to-go controls

No need to make positive or negative controls for your pGreenFire projects—SBI’s already got them built. With the pGreenFire Control Pack (available only with the purchase of any of our pGreenFire Reporter), you get the pGreenFire1-mCMV Negative Control (Cat.# TR010PA/VA-1), with both EF1α-neo and EF1α-puro versions (Cat.#s TR010PA/VA-N and Cat.# TR010PA/VA-P), and the pGreenFire1-CMV Positive Control Lentivector (Cat.# TR011PA/VA-1).

All of these vectors feature the GreenFire cassette—a destabilized copepod GFP (dscGFP; 2-hour half-life) co-expressed with luciferase. The negative controls contain only the minimal CMV promoter (mCMV) and deliver negligible expression, whereas the positive control includes the full CMV promoter for strong expression in common cell types, such as HeLa, HEK293, and HT1080.

pGreenFire1-CMV Positive Control LentivectorpGreenFire1-mCMV Negative Control LentivectorpGreenFire1-mCMV (EF1α-neo) Negative Control LentivectorpGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action


Citations

  • Heun, Y, et al. (2017) HIF-1α Dependent Wound Healing Angiogenesis In Vivo Can Be Controlled by Site-Specific Lentiviral Magnetic Targeting of SHP-2. Mol. Ther.. 2017 Jul 5; 25(7):1616-1627. PM ID: 28434868
  • Phua, KKL, Liu, Y & Sim, SH. (2017) Non-linear enhancement of mRNA delivery efficiencies by influenza A derived NS1 protein engendering host gene inhibition property. Biomaterials. 2017 Jul 1; 133:29-36. PM ID: 28426973
  • Takada, S, et al. (2017) Pluripotent stem cell models of Blau syndrome reveal an IFN-γ-dependent inflammatory response in macrophages. J. Allergy Clin. Immunol.. 2017 Jun 3;. PM ID: 28587749
  • Li, X, et al. (2017) Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy. Mol. Cell. 2017 Jun 1; 66(5):684-697.e9. PM ID: 28552616
  • Chen, YC, et al. (2017) Selective Photomechanical Detachment and Retrieval of Divided Sister Cells from Enclosed Microfluidics for Downstream Analyses. ACS Nano. 2017 May 23; 11(5):4660-4668. PM ID: 28480715
  • Choi, EJ, et al. (2017) A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer. Oncogene. 2017 May 15;. PM ID: 28504720
  • Krabbe, G, et al. (2017) Microglial NFκB-TNFα hyperactivation induces obsessive-compulsive behavior in mouse models of progranulin-deficient frontotemporal dementia. Proc. Natl. Acad. Sci. U.S.A.. 2017 May 9; 114(19):5029-5034. PM ID: 28438992
  • Schmidt, C, et al. (2017) Pre-clinical drug screen reveals topotecan, actinomycin D and volasertib as potential new therapeutic candidates for ETMR brain tumor patients. Neuro-oncology. 2017 May 8;. PM ID: 28482026
  • Pryke, KM, et al. (2017) A Novel Agonist of the TRIF Pathway Induces a Cellular State Refractory to Replication of Zika, Chikungunya, and Dengue Viruses. MBio. 2017 May 2; 8(3). PM ID: 28465426
  • Gryder, BE, et al. (2017) PAX3-FOXO1 Establishes Myogenic Super Enhancers and Confers BET Bromodomain Vulnerability. Cancer Discov. 2017 Apr 26;. PM ID: 28446439
  • Jin, C, et al. (2017) Over-expression of ASIC1a promotes proliferation via activation of the β-catenin/LEF-TCF axis and is associated with disease outcome in liver cancer. Oncotarget. 2017 Apr 18; 8(16):25977-25988. PM ID: 27462920
  • Wang, W, et al. (2017) Biological or pharmacological activation of protein kinase C alpha constrains hepatitis E virus replication. Antiviral Res.. 2017 Apr 1; 140:1-12. PM ID: 28077314
  • Teng, CT, et al. (2017) Development of Novel Cell Lines for High-Throughput Screening to Detect Estrogen-Related Receptor Alpha Modulators. SLAS Discov. 2017 Jan 1;:2472555216689772. PM ID: 28346099
  • Gujar, AD, et al. (2016) An NAD+-dependent transcriptional program governs self-renewal and radiation resistance in glioblastoma. Proc. Natl. Acad. Sci. U.S.A.. 2016 Dec 20; 113(51):E8247-E8256. PM ID: 27930300
  • Ha, SJ, et al. (2016) Preventive effect of Rhus javanica extract on UVB-induced skin inflammation and photoaging. Journal of Functional Foods. 2016 Dec 1; 27:589-599. Link: Journal of Functional Foods
  • Levy, G, et al. (2016) Nuclear receptors control pro-viral and antiviral metabolic responses to hepatitis C virus infection. Nat. Chem. Biol.. 2016 Dec 1; 12(12):1037-1045. PM ID: 27723751
  • Verma, RK, et al. (2016) α-Mangostin-encapsulated PLGA nanoparticles inhibit pancreatic carcinogenesis by targeting cancer stem cells in human, and transgenic (Kras(G12D), and Kras(G12D)/tp53R270H) mice. Sci Rep. 2016 Sep 14; 6:32743. PM ID: 27624879
  • Wang, Y, et al. (2016) 3K3A-activated protein C stimulates postischemic neuronal repair by human neural stem cells in mice. Nat. Med.. 2016 Sep 1; 22(9):1050-5. PM ID: 27548576
  • Sarett, SM, et al. (2016) Hydrophobic interactions between polymeric carrier and palmitic acid-conjugated siRNA improve PEGylated polyplex stability and enhance in vivo pharmacokinetics and tumor gene silencing. Biomaterials. 2016 Aug 1; 97:122-32. PM ID: 27163624
  • Zhang, Z, et al. (2016) Microfluidics 3D gel-island chip for single cell isolation and lineage-dependent drug responses study. Lab Chip. 2016 Jul 7; 16(13):2504-12. PM ID: 27270563