pGreenFire1-mCMV (EF1α-neo) Negative Control Lentivector

Run your pGreenFire projects with confidence with the addition of this negative control that drives copGFP and luciferase with a minimal CMV promoter (EF1α-neo)

Products

Catalog Number Description Size Price Quantity Add to Cart
TR010PA-N pGreenFire1-mCMV Plasmid (pTRH1 mCMV dscGFP T2A Fluc, negative control)+ EF1α-Neo 10 µg $562
- +
TR010VA-N pGreenFire1-mCMV Virus (pTRH1 mCMV dscGFP T2A Fluc) + EF1α-Neo >2 x 10^6 IFUs $706
- +

Overview

Overview

Supporting your studies with ready-to-go controls

No need to make a negative control for your pGreenFire projects—SBI’s already built one for you. With the pGreenFire1-mCMV (EF1α-neo) Negative Control Lentivector, the GreenFire cassette is driven by a minimal CMV promoter with destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase co-expression mediated by a T2A element. The minimal CMV promoter delivers negligible expression, and this configuration provides a control for background levels of GFP and luciferase expression in the absence of enhancer elements, such as the ones used in our Signal Transduction Pathway Reporters/Transcriptional Response Element Reporters.

pGreenFire1-mCMV (EF1α-neo) Negative Control Lentivector To simplify cell line construction, this pGreenFire1-mCMV (EF1α-neo) Negative Control Lentivector comes with a constitutively expressed neomycin marker expressed from the Ef1α promoter. Or choose an EF1α-puro (Cat.# TR010PA-P), or no marker at all (Cat.# TR010PA-1). All versions of this lentivector are available as plasmid or pre-packaged virus.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

Resources

Citations

  • Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 1970 Jan 1;. PM ID: 35483375
  • Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 1970 Jan 1;. Link: slas-discovery.org
  • Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 1970 Jan 1; 21(6):710-720. PM ID: 35606429
  • Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 1970 Jan 1;. Link: Research Square
  • Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 1970 Jan 1; 11(7). PM ID: 35884187
  • Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 1970 Jan 1; 25:101508. PM ID: 35985204
  • Chen, C, et al. (2022) ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Nature communications. 1970 Jan 1; 13(1):6108. PM ID: 36245009
  • Takase, S, et al. (2022) 17β-neriifolin from unripe fruits of Cerbera manghas suppressed cell proliferation via the inhibition of HOXA9-dependent transcription and the induction of apoptosis in the human AML cell line THP-1. Journal of natural medicines. 1970 Jan 1;. PM ID: 36266527
  • Donohue, L, et al. (2022) A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation. Cell Genomics. 1970 Jan 1;:100191. Link: Cell Genomics
  • Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 1970 Jan 1;. PM ID: 33545065
  • Deng, P, et al. (2021) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell stem cell. 1970 Jan 1;. PM ID: 33571444
  • Bencomo-Alvarez, AE, et al. (2021) Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B. Oncogene. 1970 Jan 1;. PM ID: 33712704
  • Miyashita, Y, et al. (2021) The CR9 element is a novel mechanical load-responsive enhancer that regulates natriuretic peptide genes expression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 1970 Jan 1; 35(4):e21495. PM ID: 33689182
  • Sugali, CK, et al. (2021) Full title: The canonical Wnt signaling pathway inhibits the glucocorticoid receptor signaling pathway in the trabecular meshwork. The American journal of pathology. 1970 Jan 1;. PM ID: 33705750
  • Cook, B, et al. (2021) The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3 β signaling. The Journal of biological chemistry. 1970 Jan 1;:100696. PM ID: 33895139
  • Slominski, AT, et al. (2021) Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs). Scientific reports. 1970 Jan 1; 11(1):8002. PM ID: 33850196
  • Jang, HJ, et al. (2021) Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells. Integrative cancer therapies. 1970 Jan 1; 20:15347354211006191. PM ID: 33884928
  • Gavini, C, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin-1 Expression and Sciatic Nerve-Associated Cell signaling. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Li, X, et al. (2020) Programmable base editing of mutated TERT promoter inhibits brain tumour growth. Nat. Cell Biol.. 1970 Jan 1;. PM ID: 32066906
  • Blaževitš, O, et al. (2020) MYC-Associated Factor MAX is a Regulator of the Circadian Clock. Int J Mol Sci. 1970 Jan 1; 21(7). PM ID: 32225100

Products

Catalog Number Description Size Price Quantity Add to Cart
TR010PA-N pGreenFire1-mCMV Plasmid (pTRH1 mCMV dscGFP T2A Fluc, negative control)+ EF1α-Neo 10 µg $562
- +
TR010VA-N pGreenFire1-mCMV Virus (pTRH1 mCMV dscGFP T2A Fluc) + EF1α-Neo >2 x 10^6 IFUs $706
- +

Overview

Overview

Supporting your studies with ready-to-go controls

No need to make a negative control for your pGreenFire projects—SBI’s already built one for you. With the pGreenFire1-mCMV (EF1α-neo) Negative Control Lentivector, the GreenFire cassette is driven by a minimal CMV promoter with destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase co-expression mediated by a T2A element. The minimal CMV promoter delivers negligible expression, and this configuration provides a control for background levels of GFP and luciferase expression in the absence of enhancer elements, such as the ones used in our Signal Transduction Pathway Reporters/Transcriptional Response Element Reporters.

pGreenFire1-mCMV (EF1α-neo) Negative Control Lentivector To simplify cell line construction, this pGreenFire1-mCMV (EF1α-neo) Negative Control Lentivector comes with a constitutively expressed neomycin marker expressed from the Ef1α promoter. Or choose an EF1α-puro (Cat.# TR010PA-P), or no marker at all (Cat.# TR010PA-1). All versions of this lentivector are available as plasmid or pre-packaged virus.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

Citations

  • Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 1970 Jan 1;. PM ID: 35483375
  • Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 1970 Jan 1;. Link: slas-discovery.org
  • Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 1970 Jan 1; 21(6):710-720. PM ID: 35606429
  • Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 1970 Jan 1;. Link: Research Square
  • Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 1970 Jan 1; 11(7). PM ID: 35884187
  • Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 1970 Jan 1; 25:101508. PM ID: 35985204
  • Chen, C, et al. (2022) ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Nature communications. 1970 Jan 1; 13(1):6108. PM ID: 36245009
  • Takase, S, et al. (2022) 17β-neriifolin from unripe fruits of Cerbera manghas suppressed cell proliferation via the inhibition of HOXA9-dependent transcription and the induction of apoptosis in the human AML cell line THP-1. Journal of natural medicines. 1970 Jan 1;. PM ID: 36266527
  • Donohue, L, et al. (2022) A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation. Cell Genomics. 1970 Jan 1;:100191. Link: Cell Genomics
  • Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 1970 Jan 1;. PM ID: 33545065
  • Deng, P, et al. (2021) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell stem cell. 1970 Jan 1;. PM ID: 33571444
  • Bencomo-Alvarez, AE, et al. (2021) Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B. Oncogene. 1970 Jan 1;. PM ID: 33712704
  • Miyashita, Y, et al. (2021) The CR9 element is a novel mechanical load-responsive enhancer that regulates natriuretic peptide genes expression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 1970 Jan 1; 35(4):e21495. PM ID: 33689182
  • Sugali, CK, et al. (2021) Full title: The canonical Wnt signaling pathway inhibits the glucocorticoid receptor signaling pathway in the trabecular meshwork. The American journal of pathology. 1970 Jan 1;. PM ID: 33705750
  • Cook, B, et al. (2021) The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3 β signaling. The Journal of biological chemistry. 1970 Jan 1;:100696. PM ID: 33895139
  • Slominski, AT, et al. (2021) Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs). Scientific reports. 1970 Jan 1; 11(1):8002. PM ID: 33850196
  • Jang, HJ, et al. (2021) Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells. Integrative cancer therapies. 1970 Jan 1; 20:15347354211006191. PM ID: 33884928
  • Gavini, C, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin-1 Expression and Sciatic Nerve-Associated Cell signaling. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Li, X, et al. (2020) Programmable base editing of mutated TERT promoter inhibits brain tumour growth. Nat. Cell Biol.. 1970 Jan 1;. PM ID: 32066906
  • Blaževitš, O, et al. (2020) MYC-Associated Factor MAX is a Regulator of the Circadian Clock. Int J Mol Sci. 1970 Jan 1; 21(7). PM ID: 32225100