pGF-SMAD2/3/4-mCMV-EF1α-Puro Lentivector
- Sort responsive cells with dscGFP
- Measure activity with luciferase
- Leverage SBI’s highly-regarded lentivectors
- Create stable signaling pathway reporter cell lines
- Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| TR203a-p | pGF-SMAD2/3/4-mCMV-EF1α-Puro (293 stable cell line) | 2 x 10^6 Cells | $3142 |
|
||||
| TR203pa-p | pGF-SMAD2/3/4-mCMV-EF1α-Puro (plasmid) | 10 µg | $720 |
|
||||
| TR203va-p | pGF-SMAD2/3/4-mCMV-EF1α-Puro (virus) | >2 x 10^6 IFUs | $720 |
|
||||
Overview
Overview
Monitor oncogenic signaling in real time
With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGF-SMAD2/3/4-mCMV-EF1α-Puro Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from SMAD2/3/4 transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of SMAD2/3/4 TREs, drives expression of dscGFP and luciferase in response to SMAD2/3/4 activity. The result is the ability to quantitatively measure SMAD2/3/4 activity at SMAD2/3/4 TREs by fluorescence and luciferase activity.
- Sort responsive cells with dscGFP
- Measure activity with luciferase
- Leverage SBI’s highly-regarded lentivectors
- Create stable signaling pathway reporter cell lines
- Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
The pGF-SMAD2/3/4-mCMV-EF1α-Puro Lentivector is available as a lentivector, pre-packaged virus, and a stable cell line created from the pGF-SMAD2/3/4-mCMV-EF1α-Puro Lentivector transduced into 293 cells.
*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.How It Works
Supporting Data
Supporting Data
See our transcriptional response element reporters in action
Monitor oncogenic pathway reporters
Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis
General pGreenFire data examples
Monitoring NF-κB transactivation
FAQs
Resources
Related Products
Citations
-
Ishino, T, et al. (2023) Somatic mutations can induce a noninflamed tumour microenvironment via their original gene functions, despite deriving neoantigens. British journal of cancer. 2023;. PM ID: 36732592
-
Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 2022;. PM ID: 35483375
-
Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 2022;. Link: slas-discovery.org
-
Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 2022; 21(6):710-720. PM ID: 35606429
-
Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 2022;. Link: Research Square
-
Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 2022; 11(7). PM ID: 35884187
-
Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 2022; 25:101508. PM ID: 35985204
-
Chen, C, et al. (2022) ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Nature communications. 2022; 13(1):6108. PM ID: 36245009
-
Takase, S, et al. (2022) 17β-neriifolin from unripe fruits of Cerbera manghas suppressed cell proliferation via the inhibition of HOXA9-dependent transcription and the induction of apoptosis in the human AML cell line THP-1. Journal of natural medicines. 2022;. PM ID: 36266527
-
Donohue, L, et al. (2022) A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation. Cell Genomics. 2022;:100191. Link: Cell Genomics
-
Caligiuri, SPB, et al. (2022) Hedgehog-interacting protein acts in the habenula to regulate nicotine intake. Proceedings of the National Academy of Sciences of the United States of America. 2022; 119(46):e2209870119. PM ID: 36346845
-
Tan, TG, et al. (2022) SPATA2 and CYLD inhibit T cell infiltration into colorectal cancer via regulation of IFN-γ/STAT1 axis. Frontiers in oncology. 2022; 12:1016307. PM ID: 36531014
-
Mauro-Lizcano, M, Sotgia, F & Lisanti, MP. (2022) SOX2-high cancer cells exhibit an aggressive phenotype, with increases in stemness, proliferation and invasion, as well as higher metabolic activity and ATP production. Aging. 2022; 14(24):9877-9889. PM ID: 36566021
-
Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 2021;. PM ID: 33545065
-
Deng, P, et al. (2021) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell stem cell. 2021;. PM ID: 33571444
-
Bencomo-Alvarez, AE, et al. (2021) Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B. Oncogene. 2021;. PM ID: 33712704
-
Miyashita, Y, et al. (2021) The CR9 element is a novel mechanical load-responsive enhancer that regulates natriuretic peptide genes expression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2021; 35(4):e21495. PM ID: 33689182
-
Sugali, CK, et al. (2021) Full title: The canonical Wnt signaling pathway inhibits the glucocorticoid receptor signaling pathway in the trabecular meshwork. The American journal of pathology. 2021;. PM ID: 33705750
-
Cook, B, et al. (2021) The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3 β signaling. The Journal of biological chemistry. 2021;:100696. PM ID: 33895139
-
Slominski, AT, et al. (2021) Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs). Scientific reports. 2021; 11(1):8002. PM ID: 33850196
- See More
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| TR203a-p | pGF-SMAD2/3/4-mCMV-EF1α-Puro (293 stable cell line) | 2 x 10^6 Cells | $3142 |
|
||||
| TR203pa-p | pGF-SMAD2/3/4-mCMV-EF1α-Puro (plasmid) | 10 µg | $720 |
|
||||
| TR203va-p | pGF-SMAD2/3/4-mCMV-EF1α-Puro (virus) | >2 x 10^6 IFUs | $720 |
|
||||
Overview
Overview
Monitor oncogenic signaling in real time
With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGF-SMAD2/3/4-mCMV-EF1α-Puro Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from SMAD2/3/4 transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of SMAD2/3/4 TREs, drives expression of dscGFP and luciferase in response to SMAD2/3/4 activity. The result is the ability to quantitatively measure SMAD2/3/4 activity at SMAD2/3/4 TREs by fluorescence and luciferase activity.
- Sort responsive cells with dscGFP
- Measure activity with luciferase
- Leverage SBI’s highly-regarded lentivectors
- Create stable signaling pathway reporter cell lines
- Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
The pGF-SMAD2/3/4-mCMV-EF1α-Puro Lentivector is available as a lentivector, pre-packaged virus, and a stable cell line created from the pGF-SMAD2/3/4-mCMV-EF1α-Puro Lentivector transduced into 293 cells.
*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.How It Works
Supporting Data
Supporting Data
See our transcriptional response element reporters in action
Monitor oncogenic pathway reporters
Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis
General pGreenFire data examples
Monitoring NF-κB transactivation
FAQs
Citations
-
Ishino, T, et al. (2023) Somatic mutations can induce a noninflamed tumour microenvironment via their original gene functions, despite deriving neoantigens. British journal of cancer. 2023;. PM ID: 36732592
-
Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 2022;. PM ID: 35483375
-
Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 2022;. Link: slas-discovery.org
-
Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 2022; 21(6):710-720. PM ID: 35606429
-
Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 2022;. Link: Research Square
-
Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 2022; 11(7). PM ID: 35884187
-
Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 2022; 25:101508. PM ID: 35985204
-
Chen, C, et al. (2022) ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Nature communications. 2022; 13(1):6108. PM ID: 36245009
-
Takase, S, et al. (2022) 17β-neriifolin from unripe fruits of Cerbera manghas suppressed cell proliferation via the inhibition of HOXA9-dependent transcription and the induction of apoptosis in the human AML cell line THP-1. Journal of natural medicines. 2022;. PM ID: 36266527
-
Donohue, L, et al. (2022) A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation. Cell Genomics. 2022;:100191. Link: Cell Genomics
-
Caligiuri, SPB, et al. (2022) Hedgehog-interacting protein acts in the habenula to regulate nicotine intake. Proceedings of the National Academy of Sciences of the United States of America. 2022; 119(46):e2209870119. PM ID: 36346845
-
Tan, TG, et al. (2022) SPATA2 and CYLD inhibit T cell infiltration into colorectal cancer via regulation of IFN-γ/STAT1 axis. Frontiers in oncology. 2022; 12:1016307. PM ID: 36531014
-
Mauro-Lizcano, M, Sotgia, F & Lisanti, MP. (2022) SOX2-high cancer cells exhibit an aggressive phenotype, with increases in stemness, proliferation and invasion, as well as higher metabolic activity and ATP production. Aging. 2022; 14(24):9877-9889. PM ID: 36566021
-
Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 2021;. PM ID: 33545065
-
Deng, P, et al. (2021) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell stem cell. 2021;. PM ID: 33571444
-
Bencomo-Alvarez, AE, et al. (2021) Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B. Oncogene. 2021;. PM ID: 33712704
-
Miyashita, Y, et al. (2021) The CR9 element is a novel mechanical load-responsive enhancer that regulates natriuretic peptide genes expression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2021; 35(4):e21495. PM ID: 33689182
-
Sugali, CK, et al. (2021) Full title: The canonical Wnt signaling pathway inhibits the glucocorticoid receptor signaling pathway in the trabecular meshwork. The American journal of pathology. 2021;. PM ID: 33705750
-
Cook, B, et al. (2021) The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3 β signaling. The Journal of biological chemistry. 2021;:100696. PM ID: 33895139
-
Slominski, AT, et al. (2021) Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs). Scientific reports. 2021; 11(1):8002. PM ID: 33850196
- See More