pGF-EGR1-mCMV-EF1α-Puro Lentivector

Study oncogenic signaling by co-expressing dscGFP and luciferase in response to EGR1 activity at EGR1 transcriptional response elements
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

Products

Catalog Number Description Size Price Quantity Add to Cart
TR204a-p pGF-EGR1-mCMV-EF1α-Puro (293 stable cell line) >2 x 10^6 Cells $3080
- +
Contact Us
TR204pa-p pGF-EGR1-mCMV-EF1α-Puro (plasmid) 10 µg $706
- +
Contact Us
TR204va-p pGF-EGR1-mCMV-EF1α-Puro (virus) >2 x 10^6 IFUs $706
- +
Contact Us

Overview

Overview

Monitor oncogenic signaling in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGF-EGR1-mCMV-EF1α-Puro Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from EGR1 transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of EGR1 TREs, drives expression of dscGFP and luciferase in response to EGR1 activity. The result is the ability to quantitatively measure EGR1 activity at EGR1 TREs by fluorescence and luciferase activity.
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGF-EGR1-mCMV-EF1α-Puro Lentivector

The pGF-EGR1-mCMV-EF1α-Puro Lentivector is available as a lentivector, pre-packaged virus, and a stable cell line created from the pGF-EGR1-mCMV-EF1α-Puro Lentivector transduced into 293 cells.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cells

Track and measure the activity of oncogenic signal transduction pathways in live cells

 

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

 

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

 

General pGreenFire data examples

See SBI’s pGreenFire1 reporters in action

 

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in action

 

See SBI’s pGreenFire1 reporters in action

Resources

Citations

We're sorry, an error has occurred while generating this content.

Products

Catalog Number Description Size Price Quantity Add to Cart
TR204a-p pGF-EGR1-mCMV-EF1α-Puro (293 stable cell line) >2 x 10^6 Cells $3080
- +
Contact Us
TR204pa-p pGF-EGR1-mCMV-EF1α-Puro (plasmid) 10 µg $706
- +
Contact Us
TR204va-p pGF-EGR1-mCMV-EF1α-Puro (virus) >2 x 10^6 IFUs $706
- +
Contact Us

Overview

Overview

Monitor oncogenic signaling in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGF-EGR1-mCMV-EF1α-Puro Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from EGR1 transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of EGR1 TREs, drives expression of dscGFP and luciferase in response to EGR1 activity. The result is the ability to quantitatively measure EGR1 activity at EGR1 TREs by fluorescence and luciferase activity.
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGF-EGR1-mCMV-EF1α-Puro Lentivector

The pGF-EGR1-mCMV-EF1α-Puro Lentivector is available as a lentivector, pre-packaged virus, and a stable cell line created from the pGF-EGR1-mCMV-EF1α-Puro Lentivector transduced into 293 cells.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cells

Track and measure the activity of oncogenic signal transduction pathways in live cells

 

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

 

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

 

General pGreenFire data examples

See SBI’s pGreenFire1 reporters in action

 

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in action

 

See SBI’s pGreenFire1 reporters in action

Citations

We're sorry, an error has occurred while generating this content.