pCDH-CMV-MCS-EF1α-RFP Cloning and Expression Lentivector
With the strong CMV promoter upstream of an MCS and EF1α driving the RFP reporter, pCDH-CMV-MCS-EF1α-RFP is a great, all-purpose lentivector
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CD512B-1 | pCDH-CMV-MCS-EF1α-RFP cDNA Dual Promoter Cloning and Expression Lentivector | 10 µg | $561 |
|
||||
| CD512VA-1 | pCDH-CMV-MCS-EF1α-RFP cDNA Dual Promoter Cloning and Expression Lentivector (pre-packaged virus) | >1 x 10^6 IFUs | $706 |
|
||||
Overview
Overview
Get robust, stable expression of your gene-of-interest in dividing and quiescent cells
Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-CMV-MCS-EF1α-RFP Cloning and Expression Lentivector drives expression of your gene-of-interest from the strong CMV promoter, and expression of the RFP reporter from the EF1α promoter.
With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
| Promoter | Expression Level | Application |
|---|---|---|
| CMV | High | Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.) |
| MSCV | High | Hematopoietic and stem cells |
| EF1α | Medium | Most cell types including primary cells and stem cells |
| PGK | Medium | Most cell types including primary cells and stem cells |
| UbC | Low | Most cell types including primary cells and stem cells |
How It Works
Supporting Data
Resources
Citations
-
Liu, YY, et al. (2022) O-GlcNAcylation of MORC2 at threonine 556 by OGT couples TGF-β signaling to breast cancer progression. Cell death and differentiation. 1970 Jan 1;. PM ID: 34974534
-
Lockhart-Cairns, M, et al. (2022) Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation. Matrix Biology. 1970 Jan 1;. Link: Matrix Biology
-
Ma, S, et al. (2022) PDGF-D-PDGFRβ signaling enhances IL-15-mediated human natural killer cell survival. Proceedings of the National Academy of Sciences of the United States of America. 1970 Jan 1; 119(3). PM ID: 35027451
-
Kuravi, S, et al. (2022) Functional characterization of NPM1-TYK2 fusion oncogene. NPJ precision oncology. 1970 Jan 1; 6(1):3. PM ID: 35042970
-
Kervarrec, T, et al. (2022) Recurrent FOXK1::GRHL and GPS2::GRHL fusions in trichogerminoma. The Journal of pathology. 1970 Jan 1;. PM ID: 35049062
-
Zhang, X, et al. (2022) T-Cell Immunoglobulin and Mucin Domain 1 (TIM-1) Is a Functional Entry Factor for Tick-Borne Encephalitis Virus. mBio. 1970 Jan 1;:e0286021. PM ID: 35073759
-
Richter, A, et al. (2022) The Molecular Subtype of Adult Acute Lymphoblastic Leukemia Samples Determines the Engraftment Site and Proliferation Kinetics in Patient-Derived Xenograft Models. Cells. 1970 Jan 1; 11(1). PM ID: 35011712
-
Kim, WK, et al. (2022) Heat-Shock Proteins Can Potentiate the Therapeutic Ability of Cryopreserved Mesenchymal Stem Cells for the Treatment of Acute Spinal Cord Injury in Dogs. Stem cell reviews and reports. 1970 Jan 1;. PM ID: 35001344
-
Gao, Y, et al. (2022) Golgi phosphoprotein 3 promotes angiogenesis and sorafenib resistance in hepatocellular carcinoma via upregulating exosomal miR-494-3p. Cancer cell international. 1970 Jan 1; 22(1):35. PM ID: 35073936
-
Khan, AA, et al. (2022) WBSCR22 and TRMT112 synergistically suppress cell proliferation, invasion and tumorigenesis in pancreatic cancer via transcriptional regulation of ISG15. International journal of oncology. 1970 Jan 1; 60(3). PM ID: 35088887
-
Ilina, E, et al. (2022) Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migration. iScience. 1970 Jan 1;:103842. Link: iScience
-
Kim, M, et al. (2022) A Recombinant Ig Fragment (IgCw-γεκ) Comprising the Cγ1-Cε2-4 and Cκ Domains Is an Alternative Reagent to Human IgE. Journal of immunology (Baltimore, Md. : 1950). 1970 Jan 1; 208(3):772-779. PM ID: 35022271
-
Martin, M, et al. (2022) The immune-specific E3 ubiquitin ligase MARCH1 is upregulated during HCMV infection to regulate iron levels. Journal of virology. 1970 Jan 1;:jvi0180621. PM ID: 35045264
-
Yang, B, et al. (2022) Human Cytomegalovirus Hijacks WD Repeat Domain 11 for Virion Assembly Compartment Formation and Virion Morphogenesis. Journal of virology. 1970 Jan 1;:JVI0182721. PM ID: 35020472
-
Huang, H, et al. (2022) TSPAN1-elevated FAM110A promotes pancreatic cancer progression by transcriptionally regulating HIST1H2BK. Journal of Cancer. 1970 Jan 1; 13(3):906-917. Link: Journal of Cancer
-
Vannini, I, et al. (2022) Overexpression of ultraconserved region 83- induces lung cancer tumorigenesis. PloS one. 1970 Jan 1; 17(1):e0261464. PM ID: 35015757
-
Lal, S & Raffel, C. (2022) Protocols to Manufacture an Oncolytic Measles Virus-Sensitive Immunocompetent Mouse Model of Medulloblastoma. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2423:165-177. PM ID: 34978698
-
Kono, Y, et al. (2022) Nucleoplasmic Lamin C Rapidly Accumulates at Sites of Nuclear Envelope Rupture with BAF and cGAS. bioRxiv. 1970 Jan 1;. Link: bioRxiv
-
林浩, , et al. (2022) 分泌 anti-PD-L1 及 IFNα 的间充质干细胞联合治疗小鼠黑色素瘤. 中国免疫学杂志. 1970 Jan 1;. Link: 中国免疫学杂志
-
Pan, R, et al. (2022) Augmenting NK cell-based immunotherapy by targeting mitochondrial apoptosis. Cell. 1970 Jan 1; 185(9):1521-1538.e18. PM ID: 35447071
- See More
Related Products
pCDH-CMV-MCS-EF1α-RFP Cloning and Expression Lentivector
$561.00
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CD512B-1 | pCDH-CMV-MCS-EF1α-RFP cDNA Dual Promoter Cloning and Expression Lentivector | 10 µg | $561 |
|
||||
| CD512VA-1 | pCDH-CMV-MCS-EF1α-RFP cDNA Dual Promoter Cloning and Expression Lentivector (pre-packaged virus) | >1 x 10^6 IFUs | $706 |
|
||||
Overview
Overview
Get robust, stable expression of your gene-of-interest in dividing and quiescent cells
Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-CMV-MCS-EF1α-RFP Cloning and Expression Lentivector drives expression of your gene-of-interest from the strong CMV promoter, and expression of the RFP reporter from the EF1α promoter.
With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
| Promoter | Expression Level | Application |
|---|---|---|
| CMV | High | Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.) |
| MSCV | High | Hematopoietic and stem cells |
| EF1α | Medium | Most cell types including primary cells and stem cells |
| PGK | Medium | Most cell types including primary cells and stem cells |
| UbC | Low | Most cell types including primary cells and stem cells |
How It Works
Supporting Data
Citations
-
Liu, YY, et al. (2022) O-GlcNAcylation of MORC2 at threonine 556 by OGT couples TGF-β signaling to breast cancer progression. Cell death and differentiation. 1970 Jan 1;. PM ID: 34974534
-
Lockhart-Cairns, M, et al. (2022) Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation. Matrix Biology. 1970 Jan 1;. Link: Matrix Biology
-
Ma, S, et al. (2022) PDGF-D-PDGFRβ signaling enhances IL-15-mediated human natural killer cell survival. Proceedings of the National Academy of Sciences of the United States of America. 1970 Jan 1; 119(3). PM ID: 35027451
-
Kuravi, S, et al. (2022) Functional characterization of NPM1-TYK2 fusion oncogene. NPJ precision oncology. 1970 Jan 1; 6(1):3. PM ID: 35042970
-
Kervarrec, T, et al. (2022) Recurrent FOXK1::GRHL and GPS2::GRHL fusions in trichogerminoma. The Journal of pathology. 1970 Jan 1;. PM ID: 35049062
-
Zhang, X, et al. (2022) T-Cell Immunoglobulin and Mucin Domain 1 (TIM-1) Is a Functional Entry Factor for Tick-Borne Encephalitis Virus. mBio. 1970 Jan 1;:e0286021. PM ID: 35073759
-
Richter, A, et al. (2022) The Molecular Subtype of Adult Acute Lymphoblastic Leukemia Samples Determines the Engraftment Site and Proliferation Kinetics in Patient-Derived Xenograft Models. Cells. 1970 Jan 1; 11(1). PM ID: 35011712
-
Kim, WK, et al. (2022) Heat-Shock Proteins Can Potentiate the Therapeutic Ability of Cryopreserved Mesenchymal Stem Cells for the Treatment of Acute Spinal Cord Injury in Dogs. Stem cell reviews and reports. 1970 Jan 1;. PM ID: 35001344
-
Gao, Y, et al. (2022) Golgi phosphoprotein 3 promotes angiogenesis and sorafenib resistance in hepatocellular carcinoma via upregulating exosomal miR-494-3p. Cancer cell international. 1970 Jan 1; 22(1):35. PM ID: 35073936
-
Khan, AA, et al. (2022) WBSCR22 and TRMT112 synergistically suppress cell proliferation, invasion and tumorigenesis in pancreatic cancer via transcriptional regulation of ISG15. International journal of oncology. 1970 Jan 1; 60(3). PM ID: 35088887
-
Ilina, E, et al. (2022) Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migration. iScience. 1970 Jan 1;:103842. Link: iScience
-
Kim, M, et al. (2022) A Recombinant Ig Fragment (IgCw-γεκ) Comprising the Cγ1-Cε2-4 and Cκ Domains Is an Alternative Reagent to Human IgE. Journal of immunology (Baltimore, Md. : 1950). 1970 Jan 1; 208(3):772-779. PM ID: 35022271
-
Martin, M, et al. (2022) The immune-specific E3 ubiquitin ligase MARCH1 is upregulated during HCMV infection to regulate iron levels. Journal of virology. 1970 Jan 1;:jvi0180621. PM ID: 35045264
-
Yang, B, et al. (2022) Human Cytomegalovirus Hijacks WD Repeat Domain 11 for Virion Assembly Compartment Formation and Virion Morphogenesis. Journal of virology. 1970 Jan 1;:JVI0182721. PM ID: 35020472
-
Huang, H, et al. (2022) TSPAN1-elevated FAM110A promotes pancreatic cancer progression by transcriptionally regulating HIST1H2BK. Journal of Cancer. 1970 Jan 1; 13(3):906-917. Link: Journal of Cancer
-
Vannini, I, et al. (2022) Overexpression of ultraconserved region 83- induces lung cancer tumorigenesis. PloS one. 1970 Jan 1; 17(1):e0261464. PM ID: 35015757
-
Lal, S & Raffel, C. (2022) Protocols to Manufacture an Oncolytic Measles Virus-Sensitive Immunocompetent Mouse Model of Medulloblastoma. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2423:165-177. PM ID: 34978698
-
Kono, Y, et al. (2022) Nucleoplasmic Lamin C Rapidly Accumulates at Sites of Nuclear Envelope Rupture with BAF and cGAS. bioRxiv. 1970 Jan 1;. Link: bioRxiv
-
林浩, , et al. (2022) 分泌 anti-PD-L1 及 IFNα 的间充质干细胞联合治疗小鼠黑色素瘤. 中国免疫学杂志. 1970 Jan 1;. Link: 中国免疫学杂志
-
Pan, R, et al. (2022) Augmenting NK cell-based immunotherapy by targeting mitochondrial apoptosis. Cell. 1970 Jan 1; 185(9):1521-1538.e18. PM ID: 35447071
- See More