Exosome Binding Buffer for ExoELISAs
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Overview
Overview
Additional Exosome Binding Buffer optimized for our ExoELISA Kits While each ExoELISA Kit comes with Exosome Binding Buffer, we offer this reagent for researchers who need additional Exosome Binding Buffer. Choose the exosome quantitation method that’s best for your studies| ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 ExoELISA-ULTRA GroEL | EXOCET | FluoroCet |
References
How It Works
How It Works
Our ExoELISA Kits have all the reagents you need to run the ELISA—just add lysed exosome particles. The kits are compatible with exosomes isolated using most methods, including ExoQuick®, ExoQuick-TC®, or ultracentrifugation.
The lysed exosome particles (and, thus, exosomal proteins) are directly immobilized onto the wells of the microtiter plate, and after binding, a blocking agent is added to prevent non-specific binding of the primary detection antibody (anti-CD9, -CD63, or -CD81). Following addition of the primary antibody, a secondary antibody (goat anti-rabbit) linked to horseradish peroxidase (HRP) is also added to amplify the signal and increase assay sensitivity.
The amount of the exosome marker (CD9, CD63, or CD81) is measured via activity of the bound HRP-secondary antibody using a colorimetric assay with extra-sensitive TMB as the substrate. The accumulation of colored product is proportional to the amount of marker present in each well, and is measured using a microtiter plate reader at 450 nm absorbance.
Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying the marker of interest from the ExoELISA data.
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Related Products
Products
Overview
Overview
Additional Exosome Binding Buffer optimized for our ExoELISA Kits While each ExoELISA Kit comes with Exosome Binding Buffer, we offer this reagent for researchers who need additional Exosome Binding Buffer. Choose the exosome quantitation method that’s best for your studies| ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 ExoELISA-ULTRA GroEL | EXOCET | FluoroCet |
References
How It Works
How It Works
Our ExoELISA Kits have all the reagents you need to run the ELISA—just add lysed exosome particles. The kits are compatible with exosomes isolated using most methods, including ExoQuick®, ExoQuick-TC®, or ultracentrifugation.
The lysed exosome particles (and, thus, exosomal proteins) are directly immobilized onto the wells of the microtiter plate, and after binding, a blocking agent is added to prevent non-specific binding of the primary detection antibody (anti-CD9, -CD63, or -CD81). Following addition of the primary antibody, a secondary antibody (goat anti-rabbit) linked to horseradish peroxidase (HRP) is also added to amplify the signal and increase assay sensitivity.
The amount of the exosome marker (CD9, CD63, or CD81) is measured via activity of the bound HRP-secondary antibody using a colorimetric assay with extra-sensitive TMB as the substrate. The accumulation of colored product is proportional to the amount of marker present in each well, and is measured using a microtiter plate reader at 450 nm absorbance.
Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying the marker of interest from the ExoELISA data.