ExoELISA-ULTRA Complete Kit (CD63 Detection)

With a 4-hour total assay time, this sensitive ELISA-based assay speeds quantitation of exosomes from most biofluids

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ExoELISA-ULTRA Complete Kit (CD63 detection)

96 Reactions
EXEL-ULTRA-CD63-1
$ 595
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Overview

Delivering ELISA-based exosome quantitation ULTRA fast

Improving on our popular ExoELISA Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.

Currently configured for detection of CD63, a widely recognized and popular exosomal marker1, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.

  • Sensitive—detect as little as 1 µg protein equivalent
  • Fast—complete in less than 4-hours—no more overnight incubation
  • Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
  • Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications

Choose the exosome quantitation method that’s best for your studies

  ExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet
Use For fast and sensitive antibody-based quantitation of exosomes For sensitive quantitation of exosomes when time and input sample are not limiting For fast quantitation of extracellular vesicles with moderate sample input requirements For the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection method Antibody Antibody Enzymatic Enzymatic
Quantitation chemistry Enzymatic (HRP) Enzymatic (HRP) Colorimetric Fluorescent
Total protocol time 4 hours (no overnight incubation) 24 hours 20 min 60 min
Input sample amount (protein equivalent) 1 – 200 µg >500 µg 50 µg <1 µg
Learn More ExoELISA-ULTRA CD63

ExoELISA-ULTRA CD81

ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet


REFERENCES

  1. Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.

Supporting Data

The standard curve for ExoELISA-ULTRA CD63 shows robust linearity down to ~1 x 109 exosomes.

The standard curve for ExoELISA-ULTRA CD63 provides robust linearity down to ~1 x 10^9 exosomes.


Citations

  • Cheema, AK, et al. (2018) Plasma Derived Exosomal Biomarkers of Exposure to Ionizing Radiation in Nonhuman Primates. Int J Mol Sci. 2018 Nov 1; 19(11). PM ID: 30388807
  • La Shu, S, et al. (2018) Metabolic reprogramming of stromal fibroblasts by melanoma exosome microRNA favours a pre-metastatic microenvironment. Sci Rep. 2018 Aug 27; 8(1):12905. PM ID: 30150674
  • Ruiz-de-León, MJ, et al. (2018) Lower expression of plasma-derived exosome miR-21 levels in HIV-1 elite controllers with decreasing CD4 T cell count. J Microbiol Immunol Infect. 2018 Aug 24;. PM ID: 30193823
  • Lin, M, et al. (2018) Exosomal neutral sphingomyelinase 1 suppresses hepatocellular carcinoma via decreasing the ratio of sphingomyelin/ceramide. FEBS J.. 2018 Aug 14;. PM ID: 30106227
  • Zhou, Y, et al. (2018) Exosomes from Endothelial Progenitor Cells Improve the Outcome of a Murine Model of Sepsis. Mol. Ther.. 2018 Feb 27;. PM ID: 29599080
  • Bardi, GT, Smith, MA & Hood, JL. (2018) Melanoma exosomes promote mixed M1 and M2 macrophage polarization. Cytokine. 2018 Feb 16; 105:63-72. PM ID: 29459345
  • Patel, DB, et al. (2018) Enhanced extracellular vesicle production and ethanol-mediated vascularization bioactivity via a 3D-printed scaffold-perfusion bioreactor system. Acta biomaterialia. ;. PM ID: 30471476