ExoELISA-ULTRA Complete Kit (CD63 Detection)
With a 4-hour total assay time, this sensitive ELISA-based assay speeds quantitation of exosomes from most biofluids
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXEL-ULTRA-CD63-1 | ExoELISA-ULTRA Complete Kit (CD63 detection) | 96 Reactions | $649 |
|
||||
Overview
Overview
Delivering ELISA-based exosome quantitation ULTRA fast
Improving on our popular ExoELISA™ Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.
Currently configured for detection of CD63, a widely recognized and popular exosomal marker1, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
| ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 | EXOCET | FluoroCet |
- Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.
How It Works
Supporting Data
Supporting Data
The standard curve for ExoELISA-ULTRA CD63 shows robust linearity down to ~1 x 109 exosomes.
Resources
Citations
-
Pallares-Rusiñol, A, et al. (2023) Advances in exosome analysis. Advances in clinical chemistry. 2023; 112:69-117. PM ID: 36642486
-
Otahal, A, et al. (2023) Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy. Methods in molecular biology (Clifton, N.J.). 2023; 2598:123-140. PM ID: 36355289
-
Guo, Q, et al. (2023) Glioblastoma upregulates SUMOylation of hnRNP A2/B1 to eliminate the tumor suppressor miR-204-3p, accelerating angiogenesis under hypoxia. Cell death & disease. 2023; 14(2):147. PM ID: 36810326
-
von Stade, DP, et al. (2023) Exosome cell origin affects in vitro markers of tendon repair in ovine macrophages and tenocytes. Tissue engineering. Part A. 2023;. PM ID: 36792933
-
Park, W, et al. (2023) Hydrogel Microneedles Extracting Exosomes for Early Detection of Colorectal Cancer. Biomacromolecules. 2023;. Link: Biomacromolecules
-
Roma-Rodrigues, C, Fernandes, A & Baptista, P. (2023) Exploring RAB11A Pathway to Hinder Chronic Myeloid Leukemia-Induced Angiogenesis In Vivo. Pharmaceutics. 2023; 15(3):742. Link: Pharmaceutics
-
Gandham, SK, Attarwala, HZ & Amiji, MM. (2022) Mathematical Modeling and Experimental Validation of Extracellular Vesicle-Mediated Tumor Suppressor MicroRNA Delivery and Propagation in Ovarian Cancer Cells. Molecular pharmaceutics. 2022;. PM ID: 36226722
-
Bazoer, J. (2022) Regulatory T Cell Derived EVs-Designing Novel Immune Based Therapies to Prolong Lifespan of Transplanted Tissue. Thesis. 2022;. Link: Thesis
-
Ntsethe, A & Mackraj, I. (2022) An Investigation of Exosome Concentration and Exosomal microRNA (miR-155 and miR-222) Expression in Pregnant Women with Gestational Hypertension and Preeclampsia. International journal of women's health. 2022; 14:1681-1689. PM ID: 36514348
-
Yoon, S, Bogdanov, K & Wallach, D. (2022) Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes. Cell death and differentiation. 2022;. PM ID: 34999730
-
Cilibrasi, C, et al. (2022) Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients. Biomedicines. 2022; 10(1). PM ID: 35052804
-
Melling, GE, et al. (2022) Confocal microscopy analysis reveals that only a small proportion of extracellular vesicles are successfully labelled with commonly utilised staining methods. Scientific reports. 2022; 12(1):262. PM ID: 34997141
-
Chetty, V, et al. (2022) Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell-Cell Communication in Cancer. Cancers. 2022; 14(9):2068. Link: Cancers
-
Hong, L, et al. (2022) Exosomal circular RNA hsa_circ_0006220, and hsa_circ_0001666 as biomarkers in the diagnosis of pancreatic cancer. Journal of clinical laboratory analysis. 2022;:e24447. PM ID: 35446993
-
Li, Y, et al. (2022) Mouse mesenchymal stem cell-derived exosomal miR-466f-3p reverses EMT process through inhibiting AKT/GSK3β pathway via c-MET in radiation-induced lung injury. Journal of experimental & clinical cancer research : CR. 2022; 41(1):128. PM ID: 35392967
-
Xiao, W, et al. (2022) Matrix stiffness mediates pancreatic cancer chemoresistance through induction of exosome hypersecretion in a cancer associated fibroblasts-tumor organoid biomimetic model. Matrix biology plus. 2022; 14:100111. PM ID: 35619988
-
Rodrigues, LJS. (2022) Characterization of extracellular vesicles from ascitic fluid in ovarian cancer patients. Thesis. 2022;. Link: Thesis
-
Hrdinova, T. (2022) Molecular events associated with resistance to tyrosine kinase inhibitors in leukemia cells.. Thesis. 2022;. Link: Thesis
-
Blommer, J, et al. (2022) Extracellular vesicle biomarkers for cognitive impairment in Parkinson’s disease. Brain : a journal of neurology. 2022;. PM ID: 35833836
-
Yu, HY, et al. (2022) Exosomes from PM 2.5-treated Human Bronchial Epithelial Cells Increase Lung Cancer Metastatic Potential. Biomedical and environmental sciences : BES. 2022; 35(6):473-484. PM ID: 35882407
- See More
Related Products
ExoELISA-ULTRA Complete Kit (CD63 Detection)
$649.00
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXEL-ULTRA-CD63-1 | ExoELISA-ULTRA Complete Kit (CD63 detection) | 96 Reactions | $649 |
|
||||
Overview
Overview
Delivering ELISA-based exosome quantitation ULTRA fast
Improving on our popular ExoELISA™ Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.
Currently configured for detection of CD63, a widely recognized and popular exosomal marker1, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
| ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 | EXOCET | FluoroCet |
- Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.
How It Works
Supporting Data
Supporting Data
The standard curve for ExoELISA-ULTRA CD63 shows robust linearity down to ~1 x 109 exosomes.Citations
-
Pallares-Rusiñol, A, et al. (2023) Advances in exosome analysis. Advances in clinical chemistry. 2023; 112:69-117. PM ID: 36642486
-
Otahal, A, et al. (2023) Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy. Methods in molecular biology (Clifton, N.J.). 2023; 2598:123-140. PM ID: 36355289
-
Guo, Q, et al. (2023) Glioblastoma upregulates SUMOylation of hnRNP A2/B1 to eliminate the tumor suppressor miR-204-3p, accelerating angiogenesis under hypoxia. Cell death & disease. 2023; 14(2):147. PM ID: 36810326
-
von Stade, DP, et al. (2023) Exosome cell origin affects in vitro markers of tendon repair in ovine macrophages and tenocytes. Tissue engineering. Part A. 2023;. PM ID: 36792933
-
Park, W, et al. (2023) Hydrogel Microneedles Extracting Exosomes for Early Detection of Colorectal Cancer. Biomacromolecules. 2023;. Link: Biomacromolecules
-
Roma-Rodrigues, C, Fernandes, A & Baptista, P. (2023) Exploring RAB11A Pathway to Hinder Chronic Myeloid Leukemia-Induced Angiogenesis In Vivo. Pharmaceutics. 2023; 15(3):742. Link: Pharmaceutics
-
Gandham, SK, Attarwala, HZ & Amiji, MM. (2022) Mathematical Modeling and Experimental Validation of Extracellular Vesicle-Mediated Tumor Suppressor MicroRNA Delivery and Propagation in Ovarian Cancer Cells. Molecular pharmaceutics. 2022;. PM ID: 36226722
-
Bazoer, J. (2022) Regulatory T Cell Derived EVs-Designing Novel Immune Based Therapies to Prolong Lifespan of Transplanted Tissue. Thesis. 2022;. Link: Thesis
-
Ntsethe, A & Mackraj, I. (2022) An Investigation of Exosome Concentration and Exosomal microRNA (miR-155 and miR-222) Expression in Pregnant Women with Gestational Hypertension and Preeclampsia. International journal of women's health. 2022; 14:1681-1689. PM ID: 36514348
-
Yoon, S, Bogdanov, K & Wallach, D. (2022) Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes. Cell death and differentiation. 2022;. PM ID: 34999730
-
Cilibrasi, C, et al. (2022) Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients. Biomedicines. 2022; 10(1). PM ID: 35052804
-
Melling, GE, et al. (2022) Confocal microscopy analysis reveals that only a small proportion of extracellular vesicles are successfully labelled with commonly utilised staining methods. Scientific reports. 2022; 12(1):262. PM ID: 34997141
-
Chetty, V, et al. (2022) Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell-Cell Communication in Cancer. Cancers. 2022; 14(9):2068. Link: Cancers
-
Hong, L, et al. (2022) Exosomal circular RNA hsa_circ_0006220, and hsa_circ_0001666 as biomarkers in the diagnosis of pancreatic cancer. Journal of clinical laboratory analysis. 2022;:e24447. PM ID: 35446993
-
Li, Y, et al. (2022) Mouse mesenchymal stem cell-derived exosomal miR-466f-3p reverses EMT process through inhibiting AKT/GSK3β pathway via c-MET in radiation-induced lung injury. Journal of experimental & clinical cancer research : CR. 2022; 41(1):128. PM ID: 35392967
-
Xiao, W, et al. (2022) Matrix stiffness mediates pancreatic cancer chemoresistance through induction of exosome hypersecretion in a cancer associated fibroblasts-tumor organoid biomimetic model. Matrix biology plus. 2022; 14:100111. PM ID: 35619988
-
Rodrigues, LJS. (2022) Characterization of extracellular vesicles from ascitic fluid in ovarian cancer patients. Thesis. 2022;. Link: Thesis
-
Hrdinova, T. (2022) Molecular events associated with resistance to tyrosine kinase inhibitors in leukemia cells.. Thesis. 2022;. Link: Thesis
-
Blommer, J, et al. (2022) Extracellular vesicle biomarkers for cognitive impairment in Parkinson’s disease. Brain : a journal of neurology. 2022;. PM ID: 35833836
-
Yu, HY, et al. (2022) Exosomes from PM 2.5-treated Human Bronchial Epithelial Cells Increase Lung Cancer Metastatic Potential. Biomedical and environmental sciences : BES. 2022; 35(6):473-484. PM ID: 35882407
- See More