Efficient exosome isolation from plasma
“We therefore pursued the ExoQuick® method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models1.”
When isolating exosomes from plasma, fibrinogen and fibrin can impede efficient recovery. By pre-treating plasma with thrombin, the fibrinogen can be converted to fibrin and easily pre-cleared by a simple centrifugation step. The resulting serum-like solution can now be treated with ExoQuick, original formulation, for quantitative exosome isolation that is compatible with high-throughput methods.
Want to learn more about how ExoQuick works? Find out here.
Choose the right ExoQuick for your application:
|Purest EV isolation||ExoQuick ULTRA and
|General purpose EV isolation||ExoQuick and
|EV isolation for pre-clinical/in vivo studies||ExoQuick-CG||EXOCG50A-1|
|EV isolation that removes contaminating lipoprotein particles from plasma or serum||ExoQuick-LP||EXOLP5A-1|
|EV isolation that includes a de-fibrinating plasma step prior to isolation||ExoQuick Plasma Prep with Thrombin||EXOQ5TM-1|
- Chugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.