Optimized exosomal RNA extraction for biomarker discovery and more—for serum, plasma, and ascites fluid
Looking for biomarkers? Studying exosomal RNAs (exoRNAs)? Turn to SBI’s ExoQuick Exosome Isolation and RNA Purification Kit (for Serum & Plasma) for optimized isolation of exoRNAs. Utilizing ExoQuick for efficient exosome isolation from serum, plasma, or ascites fluid, the kit also contains columns and reagents for purification of RNA from isolated exosomes. For exoRNA isolation from other biofluids, try the ExoQuick Exosome Isolation and RNA Purification Kit (for Media and Urine).
Reliable, reproducible exoRNA isolation from serum, plasma, or ascites fluid
With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related biomarkers. The ExoQuick Exosome Isolation and RNA Purification Kit (for Serum & Plasma) includes everything you need for optimized isolation of exosomal RNAs from serum, plasma, or ascites fluid samples—ExoQuick for fast and efficient exosome isolation and a phenol-free lysis buffer and rapid spin columns to extract RNA from your isolated exosomes.
How It Works
Go from sample to amplified exoRNAs in a single day
- Isolate exosomes from patient biofluids with the included ExoQuick reagent
- Purify exoRNAs with columns
Purified exoRNAs are fully compatible with most downstream applications, such as qPCR, microarray analysis, or NGS.
Isolate serum exosomes and purify exoRNAs
Tail exoRNAs and synthesize double-tagged cDNA
Use the spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.
Better qPCR profiling with ExoQuick Exosome Isolation and RNA Purification Kit (for Serum & Plasma)
Figure 1. Serum RNA prepared by the ExoQuick Exosome Isolation and RNA Purification Kit (for Serum & Plasma) delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 Human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The ExoQuick exoRNAs are compatible with downstream polyadenylation and reverse transcription reactions for amplification and accurate qPCR profiling.
Figure 2. Serum exoRNAs prepared using Exosome Isolation and RNA Purification Kit (for Serum & Plasma) deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the ExoQuick Exosome Isolation and RNA Amplification Kit (for Serum & Plasma). The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set. Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.