The Original ExoQuick

Fast, scalable exosome isolation from plasma, serum, and ascites fluid.
  • Saves time and labor
  • Is easily scalable
  • Conserves precious sample
  • Delivers high yields of functional, high quality exosomes
  • Can be used to isolate exosomes for a wide range of downstream applications, including biomarker studies, exosomal miRNA profiling, exosomal proteomics, exosomal lipidomics/metabolomics, functional studies, such as in cell-to-cell signaling, and basic biology, such as roles in tumorigenesis.

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOQ20A-1 ExoQuick exosome precipitation solution 20 mL $1083
- +
EXOQ5A-1 ExoQuick exosome precipitation solution 5 mL $335
- +

Overview

Overview

A better way to isolate exosomes

"We therefore pursued the ExoQuick® method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models1."

Need exosomes? SBI's ExoQuick, original formulation, enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 100 µl) of serum, plasma, or ascites fluid. Compatible with a wide variety of downstream applications, ExoQuick is an effective and proven alternative to ultracentrifugation1-3.

ExoQuick’s fast, ultracentrifugation-free method:
  • Saves time and labor
  • Is easily scalable
  • Conserves precious sample
  • Delivers high yields of functional, high quality exosomes
  • Can be used to isolate exosomes for a wide range of downstream applications, including biomarker studies, exosomal miRNA profiling, exosomal proteomics, exosomal lipidomics/metabolomics, functional studies, such as in cell-to-cell signaling, basic biology, such as role in tumorigenesis.
ExoQuick is a proprietary polymer that gently precipitates exosomes. Add the appropriate amount of ExoQuick to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.
BiofluidSample volumeExoQuick Volume
Serum, plasma, and ascites fluid.250 μl63 μl

In electron microscopy studies, exosomes isolated with ExoQuick appear similar to exosomes isolated using ultracentrifugation1-2, and these exosomes are also active in numerous functional assays1-3.

Exosomes isolated with ExoQuick can be used for all types of protein profiling and protein characterization studies, such as mass spectrometry, Western blotting, ELISA, and more. Higher protein yields are achieved by ExoQuick purification than by chromatography, DynaBeads, or ultracentrifugation.

Exosomes isolated with ExoQuick also provide excellent samples for studying exosome-associated nucleic acids such as microRNAs, siRNAs, and even mRNA. Quantitative analytical techniques such as qPCR, microarray studies, and next-generation sequencing are all compatible with nucleic acids isolated from ExoQuick-purified exosomes.

Backed by a growing number of publications, ExoQuick is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models.

ExoQuick exosome isolation methods are patented technologies4.

Choose the right ExoQuick for your application:
ApplicationProductCatalog #
Purest EV isolationExoQuick ULTRA and
ExoQuick-TC ULTRA
EQULTRA-20A-1
EQULTRA-20TC-1
General purpose EV isolationExoQuick and
ExoQuick-TC
EXOQ20A-1
EXOTC50A-1
EV isolation for pre-clinical/in vivo studiesExoQuick-CGEXOCG50A-1
EV isolation that removes contaminating lipoprotein particles from plasma or serumExoQuick-LPEXOLP5A-1
EV isolation that includes a de-fibrinating plasma step prior to isolationExoQuick Plasma Prep with ThrombinEXOQ5TM-1
REFERENCES
  1. Chugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.
  1. Umezu T, et al. Leukemia cell to endothelial cell communication via exosomal miRNAs. Oncogene. 2013 May 30. 32(22):2747-55. PMID: 22797057.
  1. Sohel MM, et al. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence. PLoS One. 2013 Nov 4. 8(11):e78505. PMCID: PMC3817212.
  1. Antes T, et al. Methods for Microvesicle Isolation and Selective Removal. Patent No.: US 9,005,888 B2.

How It Works

https://www.systembio.com/anti-cd63-antibody-with-goat-anti-rabbit-hrp-secondary-antibodyHow It Works

High-throughput, quantitative exosome recovery

ExoQuick can be used to purify exosomes from plasma1, serum2, and malignant ascites3. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick is an excellent option for researchers who need to purify multiple exosome samples and/or samples from small animal models or clinical research samples.

To isolate exosomes from cleared serum, plasma, or ascites fluid, simply:

  • Add an appropriate volume of ExoQuick to as little as 100 µl sample
  • Incubate for at least one hour at 4°C
  • Isolate exosomes with a 30-minute low-speed spin (1500 g).

Isolated exosomes can be found in the pellet and resuspended in an appropriate solution.

A quick and easy exosome isolation workflow

You can verify the presence of exosomes with a number of different methods, including Western blotting for general exosome markers (CD63, CD9, CD81, and HSP70), NanoSight analysis, or EM (learn about different ways to detect exosomes and more in our Exosome Basics Guide).

The Bottom Line
With ExoQuick, you can obtain high-quality exosomes from most biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample.

REFERENCES

  1. Chugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.
  1. Epple LM, et al. Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles. PLoS ONE. 2012. 7(7): e42064. PMCID: PMC3407172.
  1. As featured in: Exosome Isolation for Proteomic Analyses and RNA Profiling Douglas D. Taylor, Wolfgang Zacharias and Cicek Gercel-Taylor, Serum/Plasma Proteomics, Methods in Molecular Biology, 2011, Volume 728, Part 4, 235-246.

 

Supporting Data

Supporting Data

Use ExoQuick to isolate exosomes for proteomics and miRNA profiling studies

ExoQuick helps researchers discover protein and RNA biomarkers as well as study exosome biology by enabling fast and quantitative isolation of exosomes.

ExoQuick supports exosomal protein analysis from ascites
 
Exosomes were isolated from ovarian tumor ascites fluid using ExoQuick, chromatography, DynaBeads, or ultracentrifugation. The ExoQuick method consistently delivered higher concentrations of protein than the other three isolation methods used (Figure 1).

ExoQuick delivers high concentrations of exosomal proteins

ExoQuick delivers high concentrations of exosomal proteins

 

Figure 1. (Top panel) Exosomal proteins were extracted from recovered exosomes, and the amount of protein determined by the Bradford microassay method (Bio-Rad Laboratories), using BSA as a standard. Proteins from each exosome isolate were standardized to the original sample volume and equal volumes were applied per lane of a 12.5% SDS-PAGE gel. (Bottom panel) Western Blotting was performed to analyze the presence of the specific marker protein, placental alkaline phosphatase (PLAP). The bound immune complexes were visualized by enhanced chemiluminescence (ECL, Amersham Life Sciences) and quantitated by densitometry (Un-Scan-it Software, Silk Scientific Corp.).

ExoQuick supports high exosomal miRNA yields
 
With ExoQuick, you can quickly and easily isolate high quality exosomes for miRNA analysis (Figure 2).

Exosomal microRNAs were recovered from ovarian tumor ascites fluid using either ExoQuick isolation of exosomes followed by Trizol extraction of RNA, Trizol extraction of ovarian tumor ascites fluid with no exosome isolation, or exosome purification using DynaBeads followed by Trizol extraction of RNA. The samples where exosomes were purified using ExoQuick showed the highest yields of microRNAs (Figure 2).

ExoQuick delivers high yields of exosomal miRNA

Figure 2. Recovered RNA quality and yield was assessed using a GeneQuant II. Small RNAs were analyzed with the Agilent 2100 Bioanalyzer Lab-on-a-Chip instrument system (Agilent Technologies, Santa Clara, CA), using the Agilent Small RNA chip and reagent kit. Approximately 100 ng of isolated total RNA in 1 µl was applied to each run. The manufacturer’s recommended protocol was strictly followed to obtain Bioanalyzer profiles for the size range 6 to 150 nucleotides (nt). The profiles were calibrated for size (nt) using the small RNA ladder supplied with the kit, containing markers of 20, 40, 60, 80, and 150 nt in size, as reference. The instrument software quantitated the peak area between 0 and 150 nt as small RNA region, the area within 10 to 40 nt as miRNA region, and provides percentages of miRNA detected for each sample.

Characterizing ExoQuick exosomes with NanoSight

Exosomes purified with ExoQuick from serum show the expected particle size distribution and high concentration yields when analyzed using NanoSight’s Nanoparticle Tracking Analysis (NTA, Figure 3).

ExoQuick delivers high yields of particles consistent in size with exosomes

Figure 3.Exosome size distribution and yields from serum. Exosomes were purified from 50 pooled samples of normal human serum. 250 µl of serum was combined with 63 µl of ExoQuick, incubated at 4°C for thirty minutes, and pelleted by a 1500g spin for thirty minutes. The exosome pellet was resuspended in 100 µl of PBS, diluted 1:10,000, and visualized on the NanoSight LM10 instrument. The analysis shows that the ExoQuick isolation method recovered 90 nm exosomes at a concentration of of 2.74 x 1012 particles/ml.

Resources

Citations

  • Dhouioui, S, et al. (2022) sHLA-G as a biomarker for colorectal cancer pathogenesis. Journal of King Saud University - Science. 1970 Jan 1; 34(1):101708. Link: Journal of King Saud University - Science
  • Zhang, P, et al. (2022) circRNA circMED27 acts as a prognostic factor and mediator to promote lenvatinib resistance of hepatocellular carcinoma. Molecular Therapy - Nucleic Acids. 1970 Jan 1; 27:293-303. Link: Molecular Therapy - Nucleic Acids
  • Yu, M, et al. (2022) A novel circRNA-miRNA-mRNA network revealed exosomal circ-ATP10A as a biomarker for multiple myeloma angiogenesis. Bioengineered. 1970 Jan 1; 13(1):667-683. PM ID: 34852710
  • Ye, W, et al. (2022) Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS. Biosensors and Bioelectronics: X. 1970 Jan 1; 10:100099. Link: Biosensors and Bioelectronics: X
  • Kralj-Iglič, V, Pocsfalvi, G & Iglič, A. (2022) Morphology and Formation Mechanisms of Cellular Vesicles Harvested from Blood. Physiology. 1970 Jan 1;. Link: Physiology
  • Akbar, A, et al. (2022) Methodologies to Isolate and Purify Clinical Grade Extracellular Vesicles for Medical Applications. Cells. 1970 Jan 1; 11(2). PM ID: 35053301
  • Tan, WQ, et al. (2022) Exosome-delivered circular RNA DLGAP4 induces chemoresistance via miR-143-HK2 axis in neuroblastoma. Cancer biomarkers : section A of Disease markers. 1970 Jan 1;. PM ID: 35068445
  • Sun, Z, et al. (2022) LncRNA SNHG3 regulates the BMSC osteogenic differentiation in bone metastasis of breast cancer by modulating the miR-1273g-3p/BMP3 axis. Biochemical and biophysical research communications. 1970 Jan 1; 594:117-123. PM ID: 35081500
  • Pecankova, K, et al. (2022) Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome. PloS one. 1970 Jan 1; 17(1):e0262484. PM ID: 35007303
  • Tsutsumi, K, et al. (2022) Optimization of Isolation Method for Extracellular Vesicles from Pancreatic Juice and Impact of Protease Activity. Digestive diseases and sciences. 1970 Jan 1;. PM ID: 35037137
  • Fei, B, et al. (2022) KCNQ1OT1 inhibition alleviates high glucose-induced podocyte injury by adsorbing miR-23b-3p and regulating Sema3A. Clinical and experimental nephrology. 1970 Jan 1;. PM ID: 34997887
  • Ibrahim, S, et al. (2022) Extracellular vesicles in low volume uterine lavage and serum: novel and promising biomarker for endometritis in Arabian mares. BMC veterinary research. 1970 Jan 1; 18(1):42. PM ID: 35042518
  • Jeon, H, et al. (2022) Circulating Exosomal miR-1290 for Diagnosis of Epithelial Ovarian Cancer. Current Issues in Molecular Biology. 1970 Jan 1; 44(1):288-300. Link: Current Issues in Molecular Biology
  • Chen, X, et al. (2022) Role of Global Longitude Strain related miRNAs as potential prognostic indicators in myocardial infarction. Research Square. 1970 Jan 1;. Link: Research Square
  • Northrop-Albrecht, EJ, et al. (2022) Assessment of extracellular vesicle isolation methods from human stool supernatant. Journal of extracellular vesicles. 1970 Jan 1; 11(4):e12208. PM ID: 35383410
  • Bernareggi, D, et al. (2022) CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity. Nature communications. 1970 Jan 1; 13(1):1899. PM ID: 35393416
  • Zhang, Y, et al. (2022) Mouse circulating extracellular vesicles contain virus-derived siRNAs active in antiviral immunity. The EMBO journal. 1970 Jan 1;:e109902. PM ID: 35343600
  • Wang, D, et al. (2022) Exosomal miR-146a-5p and miR-155-5p promote CXCL12/CXCR7-induced metastasis of colorectal cancer by crosstalk with cancer-associated fibroblasts. Cell death & disease. 1970 Jan 1; 13(4):380. PM ID: 35443745
  • Wang, X, et al. (2022) Serum-derived extracellular vesicles facilitate temozolomide resistance in glioblastoma through a HOTAIR-dependent mechanism. Cell death & disease. 1970 Jan 1; 13(4):344. PM ID: 35418162
  • Wang, BD, et al. (2022) Bevacizumab attenuates osteosarcoma angiogenesis by suppressing MIAT encapsulated by serum-derived extracellular vesicles and facilitating miR-613-mediated GPR158 inhibition. Cell death & disease. 1970 Jan 1; 13(3):272. PM ID: 35347106

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOQ20A-1 ExoQuick exosome precipitation solution 20 mL $1083
- +
EXOQ5A-1 ExoQuick exosome precipitation solution 5 mL $335
- +

Overview

Overview

A better way to isolate exosomes

"We therefore pursued the ExoQuick® method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models1."

Need exosomes? SBI's ExoQuick, original formulation, enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 100 µl) of serum, plasma, or ascites fluid. Compatible with a wide variety of downstream applications, ExoQuick is an effective and proven alternative to ultracentrifugation1-3.

ExoQuick’s fast, ultracentrifugation-free method:
  • Saves time and labor
  • Is easily scalable
  • Conserves precious sample
  • Delivers high yields of functional, high quality exosomes
  • Can be used to isolate exosomes for a wide range of downstream applications, including biomarker studies, exosomal miRNA profiling, exosomal proteomics, exosomal lipidomics/metabolomics, functional studies, such as in cell-to-cell signaling, basic biology, such as role in tumorigenesis.
ExoQuick is a proprietary polymer that gently precipitates exosomes. Add the appropriate amount of ExoQuick to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.
BiofluidSample volumeExoQuick Volume
Serum, plasma, and ascites fluid.250 μl63 μl

In electron microscopy studies, exosomes isolated with ExoQuick appear similar to exosomes isolated using ultracentrifugation1-2, and these exosomes are also active in numerous functional assays1-3.

Exosomes isolated with ExoQuick can be used for all types of protein profiling and protein characterization studies, such as mass spectrometry, Western blotting, ELISA, and more. Higher protein yields are achieved by ExoQuick purification than by chromatography, DynaBeads, or ultracentrifugation.

Exosomes isolated with ExoQuick also provide excellent samples for studying exosome-associated nucleic acids such as microRNAs, siRNAs, and even mRNA. Quantitative analytical techniques such as qPCR, microarray studies, and next-generation sequencing are all compatible with nucleic acids isolated from ExoQuick-purified exosomes.

Backed by a growing number of publications, ExoQuick is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models.

ExoQuick exosome isolation methods are patented technologies4.

Choose the right ExoQuick for your application:
ApplicationProductCatalog #
Purest EV isolationExoQuick ULTRA and
ExoQuick-TC ULTRA
EQULTRA-20A-1
EQULTRA-20TC-1
General purpose EV isolationExoQuick and
ExoQuick-TC
EXOQ20A-1
EXOTC50A-1
EV isolation for pre-clinical/in vivo studiesExoQuick-CGEXOCG50A-1
EV isolation that removes contaminating lipoprotein particles from plasma or serumExoQuick-LPEXOLP5A-1
EV isolation that includes a de-fibrinating plasma step prior to isolationExoQuick Plasma Prep with ThrombinEXOQ5TM-1
REFERENCES
  1. Chugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.
  1. Umezu T, et al. Leukemia cell to endothelial cell communication via exosomal miRNAs. Oncogene. 2013 May 30. 32(22):2747-55. PMID: 22797057.
  1. Sohel MM, et al. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence. PLoS One. 2013 Nov 4. 8(11):e78505. PMCID: PMC3817212.
  1. Antes T, et al. Methods for Microvesicle Isolation and Selective Removal. Patent No.: US 9,005,888 B2.

How It Works

https://www.systembio.com/anti-cd63-antibody-with-goat-anti-rabbit-hrp-secondary-antibodyHow It Works

High-throughput, quantitative exosome recovery

ExoQuick can be used to purify exosomes from plasma1, serum2, and malignant ascites3. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick is an excellent option for researchers who need to purify multiple exosome samples and/or samples from small animal models or clinical research samples.

To isolate exosomes from cleared serum, plasma, or ascites fluid, simply:

  • Add an appropriate volume of ExoQuick to as little as 100 µl sample
  • Incubate for at least one hour at 4°C
  • Isolate exosomes with a 30-minute low-speed spin (1500 g).

Isolated exosomes can be found in the pellet and resuspended in an appropriate solution.

A quick and easy exosome isolation workflow

You can verify the presence of exosomes with a number of different methods, including Western blotting for general exosome markers (CD63, CD9, CD81, and HSP70), NanoSight analysis, or EM (learn about different ways to detect exosomes and more in our Exosome Basics Guide).

The Bottom Line
With ExoQuick, you can obtain high-quality exosomes from most biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample.

REFERENCES

  1. Chugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.
  1. Epple LM, et al. Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles. PLoS ONE. 2012. 7(7): e42064. PMCID: PMC3407172.
  1. As featured in: Exosome Isolation for Proteomic Analyses and RNA Profiling Douglas D. Taylor, Wolfgang Zacharias and Cicek Gercel-Taylor, Serum/Plasma Proteomics, Methods in Molecular Biology, 2011, Volume 728, Part 4, 235-246.

 

Supporting Data

Supporting Data

Use ExoQuick to isolate exosomes for proteomics and miRNA profiling studies

ExoQuick helps researchers discover protein and RNA biomarkers as well as study exosome biology by enabling fast and quantitative isolation of exosomes.

ExoQuick supports exosomal protein analysis from ascites
 
Exosomes were isolated from ovarian tumor ascites fluid using ExoQuick, chromatography, DynaBeads, or ultracentrifugation. The ExoQuick method consistently delivered higher concentrations of protein than the other three isolation methods used (Figure 1).

ExoQuick delivers high concentrations of exosomal proteins

ExoQuick delivers high concentrations of exosomal proteins

 

Figure 1. (Top panel) Exosomal proteins were extracted from recovered exosomes, and the amount of protein determined by the Bradford microassay method (Bio-Rad Laboratories), using BSA as a standard. Proteins from each exosome isolate were standardized to the original sample volume and equal volumes were applied per lane of a 12.5% SDS-PAGE gel. (Bottom panel) Western Blotting was performed to analyze the presence of the specific marker protein, placental alkaline phosphatase (PLAP). The bound immune complexes were visualized by enhanced chemiluminescence (ECL, Amersham Life Sciences) and quantitated by densitometry (Un-Scan-it Software, Silk Scientific Corp.).

ExoQuick supports high exosomal miRNA yields
 
With ExoQuick, you can quickly and easily isolate high quality exosomes for miRNA analysis (Figure 2).

Exosomal microRNAs were recovered from ovarian tumor ascites fluid using either ExoQuick isolation of exosomes followed by Trizol extraction of RNA, Trizol extraction of ovarian tumor ascites fluid with no exosome isolation, or exosome purification using DynaBeads followed by Trizol extraction of RNA. The samples where exosomes were purified using ExoQuick showed the highest yields of microRNAs (Figure 2).

ExoQuick delivers high yields of exosomal miRNA

Figure 2. Recovered RNA quality and yield was assessed using a GeneQuant II. Small RNAs were analyzed with the Agilent 2100 Bioanalyzer Lab-on-a-Chip instrument system (Agilent Technologies, Santa Clara, CA), using the Agilent Small RNA chip and reagent kit. Approximately 100 ng of isolated total RNA in 1 µl was applied to each run. The manufacturer’s recommended protocol was strictly followed to obtain Bioanalyzer profiles for the size range 6 to 150 nucleotides (nt). The profiles were calibrated for size (nt) using the small RNA ladder supplied with the kit, containing markers of 20, 40, 60, 80, and 150 nt in size, as reference. The instrument software quantitated the peak area between 0 and 150 nt as small RNA region, the area within 10 to 40 nt as miRNA region, and provides percentages of miRNA detected for each sample.

Characterizing ExoQuick exosomes with NanoSight

Exosomes purified with ExoQuick from serum show the expected particle size distribution and high concentration yields when analyzed using NanoSight’s Nanoparticle Tracking Analysis (NTA, Figure 3).

ExoQuick delivers high yields of particles consistent in size with exosomes

Figure 3.Exosome size distribution and yields from serum. Exosomes were purified from 50 pooled samples of normal human serum. 250 µl of serum was combined with 63 µl of ExoQuick, incubated at 4°C for thirty minutes, and pelleted by a 1500g spin for thirty minutes. The exosome pellet was resuspended in 100 µl of PBS, diluted 1:10,000, and visualized on the NanoSight LM10 instrument. The analysis shows that the ExoQuick isolation method recovered 90 nm exosomes at a concentration of of 2.74 x 1012 particles/ml.

Citations

  • Dhouioui, S, et al. (2022) sHLA-G as a biomarker for colorectal cancer pathogenesis. Journal of King Saud University - Science. 1970 Jan 1; 34(1):101708. Link: Journal of King Saud University - Science
  • Zhang, P, et al. (2022) circRNA circMED27 acts as a prognostic factor and mediator to promote lenvatinib resistance of hepatocellular carcinoma. Molecular Therapy - Nucleic Acids. 1970 Jan 1; 27:293-303. Link: Molecular Therapy - Nucleic Acids
  • Yu, M, et al. (2022) A novel circRNA-miRNA-mRNA network revealed exosomal circ-ATP10A as a biomarker for multiple myeloma angiogenesis. Bioengineered. 1970 Jan 1; 13(1):667-683. PM ID: 34852710
  • Ye, W, et al. (2022) Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS. Biosensors and Bioelectronics: X. 1970 Jan 1; 10:100099. Link: Biosensors and Bioelectronics: X
  • Kralj-Iglič, V, Pocsfalvi, G & Iglič, A. (2022) Morphology and Formation Mechanisms of Cellular Vesicles Harvested from Blood. Physiology. 1970 Jan 1;. Link: Physiology
  • Akbar, A, et al. (2022) Methodologies to Isolate and Purify Clinical Grade Extracellular Vesicles for Medical Applications. Cells. 1970 Jan 1; 11(2). PM ID: 35053301
  • Tan, WQ, et al. (2022) Exosome-delivered circular RNA DLGAP4 induces chemoresistance via miR-143-HK2 axis in neuroblastoma. Cancer biomarkers : section A of Disease markers. 1970 Jan 1;. PM ID: 35068445
  • Sun, Z, et al. (2022) LncRNA SNHG3 regulates the BMSC osteogenic differentiation in bone metastasis of breast cancer by modulating the miR-1273g-3p/BMP3 axis. Biochemical and biophysical research communications. 1970 Jan 1; 594:117-123. PM ID: 35081500
  • Pecankova, K, et al. (2022) Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome. PloS one. 1970 Jan 1; 17(1):e0262484. PM ID: 35007303
  • Tsutsumi, K, et al. (2022) Optimization of Isolation Method for Extracellular Vesicles from Pancreatic Juice and Impact of Protease Activity. Digestive diseases and sciences. 1970 Jan 1;. PM ID: 35037137
  • Fei, B, et al. (2022) KCNQ1OT1 inhibition alleviates high glucose-induced podocyte injury by adsorbing miR-23b-3p and regulating Sema3A. Clinical and experimental nephrology. 1970 Jan 1;. PM ID: 34997887
  • Ibrahim, S, et al. (2022) Extracellular vesicles in low volume uterine lavage and serum: novel and promising biomarker for endometritis in Arabian mares. BMC veterinary research. 1970 Jan 1; 18(1):42. PM ID: 35042518
  • Jeon, H, et al. (2022) Circulating Exosomal miR-1290 for Diagnosis of Epithelial Ovarian Cancer. Current Issues in Molecular Biology. 1970 Jan 1; 44(1):288-300. Link: Current Issues in Molecular Biology
  • Chen, X, et al. (2022) Role of Global Longitude Strain related miRNAs as potential prognostic indicators in myocardial infarction. Research Square. 1970 Jan 1;. Link: Research Square
  • Northrop-Albrecht, EJ, et al. (2022) Assessment of extracellular vesicle isolation methods from human stool supernatant. Journal of extracellular vesicles. 1970 Jan 1; 11(4):e12208. PM ID: 35383410
  • Bernareggi, D, et al. (2022) CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity. Nature communications. 1970 Jan 1; 13(1):1899. PM ID: 35393416
  • Zhang, Y, et al. (2022) Mouse circulating extracellular vesicles contain virus-derived siRNAs active in antiviral immunity. The EMBO journal. 1970 Jan 1;:e109902. PM ID: 35343600
  • Wang, D, et al. (2022) Exosomal miR-146a-5p and miR-155-5p promote CXCL12/CXCR7-induced metastasis of colorectal cancer by crosstalk with cancer-associated fibroblasts. Cell death & disease. 1970 Jan 1; 13(4):380. PM ID: 35443745
  • Wang, X, et al. (2022) Serum-derived extracellular vesicles facilitate temozolomide resistance in glioblastoma through a HOTAIR-dependent mechanism. Cell death & disease. 1970 Jan 1; 13(4):344. PM ID: 35418162
  • Wang, BD, et al. (2022) Bevacizumab attenuates osteosarcoma angiogenesis by suppressing MIAT encapsulated by serum-derived extracellular vesicles and facilitating miR-613-mediated GPR158 inhibition. Cell death & disease. 1970 Jan 1; 13(3):272. PM ID: 35347106