Exo-FITC Exosome FACS Stain

A FITC-based stain to visualize exosomes immobilized to Exo-Flow beads for FACS and other imaging applications

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Exo-FITC Exosome FACS stain

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Exo-FITC Exosome FACS stain

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20 Reactions
EXOFLOW800A-1
$ 169
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Overview

Visualize bead-immobilized exosomes with our proprietary, reversible FITC-based stain

Optimized for our Exo-Flow Capture Kits and available only from SBI, Exo-FITC is a proprietary reversible fluorescent stain for visualizing exosomes. Exo-FITC is comprised of the commonly used fluorophore fluorescein isothiocyanate (FITC) conjugated to a protein that recognizes post-translational modifications on exosomal surface proteins, enabling general staining of exosomes.

However, the feature that really makes Exo-FITC stand out as an ideal reagent for FACS-based isolation of exosomes is its reversibility—after staining with Exo-FITC and flow-sorting by FITC-positive gating (Exo-FITC’s excitation and emission wavelengths are 494 nm and 518 nm, respectively), Exo-FITC can be removed from exosomes through the simple addition of Exosome Elution Buffer, which simultaneously elutes exosomes from the Exo-Flow magnetic beads and removes bound Exo-FITC.

Proprietary, reversible Exo-FITC is optimized for Exo-Flow Capture Kits

Note that we also offer reversible Exo-APC (Cat.# Exo-FLOW810A-1) as an alternative stain to Exo-FITC.

Use Exo-FITC with any of our Exo-Flow Capture Kits:

Cat.# Kit
EXOFLOW100A-1 CD9 Exo-Flow Capture Kit
EXOFLOW150A-1 Tetraspanin Exo-Flow Combo Capture Kit

EXOFLOW200A-1 CD31 Exo-Flow Capture Kit
EXOFLOW210A-1 CD44 Exo-Flow Capture Kit
EXOFLOW300A-1 CD63 Exo-Flow Capture Kit
EXOFLOW400A-1 CD81 Exo-Flow Capture Kit
EXOFLOW500A-1 Rab5b Exo-Flow Capture Kit
EXOFLOW600A-1 HLA-G Exo-Flow Capture Kit
EXOFLOW610A-1 CD14 Exo-Flow Capture Kit
EXOFLOW620A-1 CD68 Exo-Flow Capture Kit
EXOFLOW630A-1 EpCAM Exo-Flow Capture Kit
EXOFLOW650A-1 α2-Macroglobulin (A2M) Exo-Flow Capture Kit
EXOFLOW660A-1 CD73 Exo-Flow Capture Kit

How It Works

Easily purify exosomes using FACS with our Exo-Flow Capture Kits

At a glance
Simply (1) couple biotinylated antibody to the magnetic streptavidin beads, (2) use the antibody-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-FITC (excitation and emission wavelengths of 494 nm and 518 nm, respectively).

Your sample is now ready for FACS analysis.

To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-FITC stain and elute intact exosomes from the beads.

How the Exo-Flow Capture Kit’s magnetic stand works

Supporting Data

See Exo-FITC in action

Highly selective exosome isolation by FACS using Exo-FITC stain

Exo-FITC enables selective exosome isolation via FACS. Exosomes are isolated using the CD9 Exo-Flow Capture Kit and flow-sorted using FITC-positive gating.


Citations

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