pGreenPuro (CMV) shRNA Cloning and Expression Lentivector

Set up stable, heritable gene silencing with this shRNA HIV-based lentivector – co-express puromycin and copGFP from the CMV promoter

Products

Catalog Number Description Size Price Quantity Add to Cart
SI505A-1 pGreenPuro shRNA expression lentivector (CMV) 10 µg $582
- +
SI505VB-1 pGreenPuro shRNA expression lentivector (CMV; pre-packaged virus) >1 x 10^6 IFUs $639
- +

Overview

Overview

Set up stable, heritable RNAi

Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors also efficiently deliver RNAi. Generate cell lines with stable, heritable gene silencing to develop a thorough understand of the target gene’s function. Our HIV-based pGreenPuro (CMV) shRNA Cloning and Expression Lentivector shRNA Cloning and Expression Lentivector drives expression of your shRNA template from the H1 promoter, and after processing in the cell, your shRNA will be converted into siRNA. The vector also co-expresses puromycin and copGFP from the strong CMV promoter, with co-expression mediated by a T2A element.

pGreenPuro (CMV) shRNA Cloning and Expression Lentivector

How It Works

How It Works

Using SBI’s shRNA lentivectors to produce siRNAs

To produce siRNAs for RNAi using the pSIF1-H1-H2Kk Cloning and Expression Lentivector, first clone your shRNA template into the unique BamHI or EcoRI sites in the vector. After packaging and transduction, the vector will integrate into the genome and your shRNA will be transcribed from the H1 promoter using RNA polymerase III. The shRNA is transcribed as a single strand with a sense-loop-anti-sense structure that folds into a hairpin, and is then processed by DICER to produce an active siRNA molecule (Figure 1).

Generating siRNA from the pGreenPuro (CMV) shRNA Cloning and Expression Lentivector

Figure 1. Generating siRNA from the pGreenPuro (CMV) shRNA Cloning and Expression Lentivector.

Supporting Data

Supporting Data

Using SBI’s shRNA lentivectors—selecting for transductants

Using SBI’s shRNA lentivectors—selecting for transductants

Figure 2. Using SBI’s shRNA lentivectors. Easily select transductants with one of our markers—these examples show selection using GFP and puromycin markers on either the pSIH1-H1-copGFP (Cat.# SI501B-1) or pGreenPuro™ (Cat.# SI505A-1VB-1) shRNA Cloning and Expression Lentivectors.

Resources

Citations

  • Zhang, R, et al. (2022) LncRNA SNHG1 promotes sepsis-induced myocardial injury by inhibiting Bcl-2 expression via DNMT1. Journal of cellular and molecular medicine. 1970 Jan 1;. PM ID: 35678255
  • Liu, HC, Zhu, WY & Ren, LY. (2022) LncRNA H19 inhibits proliferation and enhances apoptosis of nephroblastoma cells by regulating the miR-675/TGFBI axis. European review for medical and pharmacological sciences. 1970 Jan 1; 26(11):3800-3806. PM ID: 35731049
  • Agit, B. (2022) Endogenous retroviral proteins as potential drug targets for merlin-deficient tumours. Thesis. 1970 Jan 1;. Link: Thesis
  • Zhu, X, et al. (2022) DNMT3B-mediated FAM111B methylation promotes papillary thyroid tumor glycolysis, growth and metastasis. ijbs.com. 1970 Jan 1;. Link: ijbs.com
  • Wang, Q, et al. (2022) Fbxo45-mediated NP-STEP46 degradation via K6-linked ubiquitination sustains ERK activity in lung cancer. Molecular oncology. 1970 Jan 1;. PM ID: 35838331
  • Ye, Q, et al. (2022) Let-7b-5p inhibits breast cancer cell growth and metastasis via repression of hexokinase 2-mediated aerobic glycolysis. Research Square. 1970 Jan 1;. Link: Research Square
  • Zhang, M, et al. (2022) Endothelial cells regulated by RNF20 orchestrate the proliferation and differentiation of neural precursor cells during embryonic development. Cell reports. 1970 Jan 1; 40(11):111350. PM ID: 36103829
  • Athans, S, et al. (2022) STAG2 expression is associated with adverse survival outcomes and regulates cell phenotype in muscle-invasive bladder cancer. Cancer research communications. 1970 Jan 1; 2(10):1129-1143. PM ID: 36275363
  • Lu, W, et al. (2021) SUMOylation is essential for Sirt2 tumor-suppressor function in neuroblastoma. Neoplasia (New York, N.Y.). 1970 Jan 1; 23(1):129-139. PM ID: 33316537
  • Meng, P, et al. (2021) Identification of the atypical cadherin FAT1 as a novel glypican-3 interacting protein in liver cancer cells. Scientific reports. 1970 Jan 1; 11(1):40. PM ID: 33420124
  • Liao, Y, et al. (2021) OLIG2 maintenance is not essential for diffuse intrinsic pontine glioma cell line growth but regulates tumor phenotypes. Neuro-oncology. 1970 Jan 1;. PM ID: 33539525
  • Zhang, Y, et al. (2021) Small extracellular vesicles ameliorate peripheral neuropathy and enhance chemotherapy of oxaliplatin on ovarian cancer. Journal of extracellular vesicles. 1970 Jan 1; 10(5):e12073. PM ID: 33728031
  • Robinson, AD. (2021) Biomarker and Target Discovery in Cancer. Thesis. 1970 Jan 1;. Link: Thesis
  • Yang, Y, et al. (2021) Programmed death ligand-1 regulates angiogenesis and metastasis by participating in the c-JUN/VEGFR2 signaling axis in ovarian cancer. Cancer communications (London, England). 1970 Jan 1;. PM ID: 33939321
  • Robinson, AD, et al. (2021) Collagen modifying enzyme P4HA1 is overexpressed and plays a role in lung adenocarcinoma. Translational oncology. 1970 Jan 1; 14(8):101128. PM ID: 34049151
  • Gao, Y, et al. (2021) NLRC4 activation needs lncRNA LNCGM1082. Research Square. 1970 Jan 1;. Link: Research Square
  • Lin, K, et al. (2021) MHC class I H2-Kb negatively regulates neural progenitor cell proliferation by inhibiting FGFR signaling. PLoS biology. 1970 Jan 1; 19(6):e3001311. PM ID: 34181639
  • Xin, R, et al. (2021) Downregulation of miR-23b by transcription factor c-Myc alleviates ischemic brain injury by upregulating Nrf2. International journal of biological sciences. 1970 Jan 1; 17(13):3659-3671. PM ID: 34512173
  • Wang, Z, et al. (2021) Knockdown of LINC01385 inhibits osteoarthritis progression by modulating the microRNA-140-3p/TLR4 axis. Experimental and therapeutic medicine. 1970 Jan 1; 22(5):1244. PM ID: 34539840
  • Zhong, M, et al. (2021) Natural Compound Library Screening Identifies Sanguinarine Chloride For The Treatment of SCLC By Upregulating CDKN1A. Research Square. 1970 Jan 1;. Link: Research Square

Products

Catalog Number Description Size Price Quantity Add to Cart
SI505A-1 pGreenPuro shRNA expression lentivector (CMV) 10 µg $582
- +
SI505VB-1 pGreenPuro shRNA expression lentivector (CMV; pre-packaged virus) >1 x 10^6 IFUs $639
- +

Overview

Overview

Set up stable, heritable RNAi

Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors also efficiently deliver RNAi. Generate cell lines with stable, heritable gene silencing to develop a thorough understand of the target gene’s function. Our HIV-based pGreenPuro (CMV) shRNA Cloning and Expression Lentivector shRNA Cloning and Expression Lentivector drives expression of your shRNA template from the H1 promoter, and after processing in the cell, your shRNA will be converted into siRNA. The vector also co-expresses puromycin and copGFP from the strong CMV promoter, with co-expression mediated by a T2A element.

pGreenPuro (CMV) shRNA Cloning and Expression Lentivector

How It Works

How It Works

Using SBI’s shRNA lentivectors to produce siRNAs

To produce siRNAs for RNAi using the pSIF1-H1-H2Kk Cloning and Expression Lentivector, first clone your shRNA template into the unique BamHI or EcoRI sites in the vector. After packaging and transduction, the vector will integrate into the genome and your shRNA will be transcribed from the H1 promoter using RNA polymerase III. The shRNA is transcribed as a single strand with a sense-loop-anti-sense structure that folds into a hairpin, and is then processed by DICER to produce an active siRNA molecule (Figure 1).

Generating siRNA from the pGreenPuro (CMV) shRNA Cloning and Expression Lentivector

Figure 1. Generating siRNA from the pGreenPuro (CMV) shRNA Cloning and Expression Lentivector.

Supporting Data

Supporting Data

Using SBI’s shRNA lentivectors—selecting for transductants

Using SBI’s shRNA lentivectors—selecting for transductants

Figure 2. Using SBI’s shRNA lentivectors. Easily select transductants with one of our markers—these examples show selection using GFP and puromycin markers on either the pSIH1-H1-copGFP (Cat.# SI501B-1) or pGreenPuro™ (Cat.# SI505A-1VB-1) shRNA Cloning and Expression Lentivectors.

Citations

  • Zhang, R, et al. (2022) LncRNA SNHG1 promotes sepsis-induced myocardial injury by inhibiting Bcl-2 expression via DNMT1. Journal of cellular and molecular medicine. 1970 Jan 1;. PM ID: 35678255
  • Liu, HC, Zhu, WY & Ren, LY. (2022) LncRNA H19 inhibits proliferation and enhances apoptosis of nephroblastoma cells by regulating the miR-675/TGFBI axis. European review for medical and pharmacological sciences. 1970 Jan 1; 26(11):3800-3806. PM ID: 35731049
  • Agit, B. (2022) Endogenous retroviral proteins as potential drug targets for merlin-deficient tumours. Thesis. 1970 Jan 1;. Link: Thesis
  • Zhu, X, et al. (2022) DNMT3B-mediated FAM111B methylation promotes papillary thyroid tumor glycolysis, growth and metastasis. ijbs.com. 1970 Jan 1;. Link: ijbs.com
  • Wang, Q, et al. (2022) Fbxo45-mediated NP-STEP46 degradation via K6-linked ubiquitination sustains ERK activity in lung cancer. Molecular oncology. 1970 Jan 1;. PM ID: 35838331
  • Ye, Q, et al. (2022) Let-7b-5p inhibits breast cancer cell growth and metastasis via repression of hexokinase 2-mediated aerobic glycolysis. Research Square. 1970 Jan 1;. Link: Research Square
  • Zhang, M, et al. (2022) Endothelial cells regulated by RNF20 orchestrate the proliferation and differentiation of neural precursor cells during embryonic development. Cell reports. 1970 Jan 1; 40(11):111350. PM ID: 36103829
  • Athans, S, et al. (2022) STAG2 expression is associated with adverse survival outcomes and regulates cell phenotype in muscle-invasive bladder cancer. Cancer research communications. 1970 Jan 1; 2(10):1129-1143. PM ID: 36275363
  • Lu, W, et al. (2021) SUMOylation is essential for Sirt2 tumor-suppressor function in neuroblastoma. Neoplasia (New York, N.Y.). 1970 Jan 1; 23(1):129-139. PM ID: 33316537
  • Meng, P, et al. (2021) Identification of the atypical cadherin FAT1 as a novel glypican-3 interacting protein in liver cancer cells. Scientific reports. 1970 Jan 1; 11(1):40. PM ID: 33420124
  • Liao, Y, et al. (2021) OLIG2 maintenance is not essential for diffuse intrinsic pontine glioma cell line growth but regulates tumor phenotypes. Neuro-oncology. 1970 Jan 1;. PM ID: 33539525
  • Zhang, Y, et al. (2021) Small extracellular vesicles ameliorate peripheral neuropathy and enhance chemotherapy of oxaliplatin on ovarian cancer. Journal of extracellular vesicles. 1970 Jan 1; 10(5):e12073. PM ID: 33728031
  • Robinson, AD. (2021) Biomarker and Target Discovery in Cancer. Thesis. 1970 Jan 1;. Link: Thesis
  • Yang, Y, et al. (2021) Programmed death ligand-1 regulates angiogenesis and metastasis by participating in the c-JUN/VEGFR2 signaling axis in ovarian cancer. Cancer communications (London, England). 1970 Jan 1;. PM ID: 33939321
  • Robinson, AD, et al. (2021) Collagen modifying enzyme P4HA1 is overexpressed and plays a role in lung adenocarcinoma. Translational oncology. 1970 Jan 1; 14(8):101128. PM ID: 34049151
  • Gao, Y, et al. (2021) NLRC4 activation needs lncRNA LNCGM1082. Research Square. 1970 Jan 1;. Link: Research Square
  • Lin, K, et al. (2021) MHC class I H2-Kb negatively regulates neural progenitor cell proliferation by inhibiting FGFR signaling. PLoS biology. 1970 Jan 1; 19(6):e3001311. PM ID: 34181639
  • Xin, R, et al. (2021) Downregulation of miR-23b by transcription factor c-Myc alleviates ischemic brain injury by upregulating Nrf2. International journal of biological sciences. 1970 Jan 1; 17(13):3659-3671. PM ID: 34512173
  • Wang, Z, et al. (2021) Knockdown of LINC01385 inhibits osteoarthritis progression by modulating the microRNA-140-3p/TLR4 axis. Experimental and therapeutic medicine. 1970 Jan 1; 22(5):1244. PM ID: 34539840
  • Zhong, M, et al. (2021) Natural Compound Library Screening Identifies Sanguinarine Chloride For The Treatment of SCLC By Upregulating CDKN1A. Research Square. 1970 Jan 1;. Link: Research Square