pGreenPuro Scramble Hairpin Control (EF1α) shRNA Expression Lentivector
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| SI506-000PA-1 | pGreenPuro Scramble Hairpin Control - Construct (EF1) | 10 µg | $594 |
|
||||
Overview
Overview
Set up stable, heritable RNAi
Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors also efficiently deliver RNAi. Generate cell lines with stable, heritable gene silencing to develop a throrough understand of the target gene’s function. Our HIV-based pGreenPuro (EF1α) shRNA Cloning and Expression Lentivector shRNA Cloning and Expression Lentivector drives expression of your shRNA template from the H1 promoter, and after processing in the cell, your shRNA will be converted into siRNA. The vector also co-expresses puromycin and copGFP from the moderate EF1α promoter, with co-expression mediated by a T2A element.
How It Works
How It Works
Using SBI’s shRNA lentivectors to produce siRNAs
To produce siRNAs for RNAi using the pSIF1-H1-H2Kk Cloning and Expression Lentivector, first clone your shRNA template into the unique BamHI or EcoRI sites in the vector. After packaging and transduction, the vector will integrate into the genome and your shRNA will be transcribed from the H1 promoter using RNA polymerase III. The shRNA is transcribed as a single strand with a sense-loop-anti-sense structure that folds into a hairpin, and is then processed by DICER to produce an active siRNA molecule (Figure 1).
Figure 1. Generating siRNA from the pGreenPuro (EF1α) shRNA Cloning and Expression Lentivector.
Supporting Data
Supporting Data
Using SBI’s shRNA lentivectors—selecting for transductants
Figure 2. Using SBI’s shRNA lentivectors. Easily select transductants with one of our markers—these examples show selection using GFP and puromycin markers on either the pSIH1-H1-copGFP (Cat.# SI501B-1) or pGreenPuro™ (Cat.# SI505A-1VB-1) shRNA Cloning and Expression Lentivectors.
FAQs
Resources
Related Products
Citations
-
Zeng, ZC, et al. (2023) METTL3 protects METTL14 from STUB1-mediated degradation to maintain m6 A homeostasis. EMBO reports. 2023;:e55762. PM ID: 36597993
-
Sierra-Magro, A, et al. (2023) C/EBPβ Regulates TFAM Expression, Mitochondrial Function and Autophagy in Cellular Models of Parkinson’s Disease. International journal of molecular sciences. 2023; 24(2). PM ID: 36674978
-
Ma, J, et al. (2023) Enhanced E6AP-mediated ubiquitination of ENO1 via LINC00663 contributes to radiosensitivity of breast cancer by regulating mitochondrial homeostasis. Cancer Letters. 2023;:216118. Link: Cancer Letters
-
Wen, J, et al. (2023) Lnc-17Rik promotes the immunosuppressive function of Myeloid-Derived suppressive cells in esophageal cancer. Cellular immunology. 2023; 385:104676. PM ID: 36780770
-
Hasegawa, S, Imai, M & Takahashi, N. (2023) Role of acetoacetyl-CoA synthetase in glucose uptake by HepG2 cells. bioRxiv. 2023;. Link: bioRxiv
-
Agarwal, S, et al. (2023) BZW2 Inhibition Reduces Colorectal Cancer Growth and Metastasis. Molecular cancer research : MCR. 2023;:OF1-OF15. PM ID: 37067340
-
Song, S, et al. (2023) CHMP4A stimulates CD8+ T-lymphocyte infiltration and inhibits breast tumor growth via the LSD1/IFNβ axis. Cancer science. 2023;. PM ID: 37198999
-
Shaker, BT, et al. (2023) The 14-Kilodalton Human Growth Hormone Fragment a Potent Inhibitor of Angiogenesis and Tumor Metastasis. International journal of molecular sciences. 2023; 24(10). PM ID: 37240223
-
Ju, Z, et al. (2023) TXNL4B regulates radioresistance by controlling the PRP3-mediated alternative splicing of FANCI. MedComm. 2023; 4(3):e258. PM ID: 37168687
-
Salehi, S, et al. (2023) Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr. Nature communications. 2023; 14(1):4158. PM ID: 37438340
-
Zhang, B, et al. (2023) WIF1 promoter hypermethylation induce endometrial carcinogenesis through the Wnt/β-catenin signaling pathway. American journal of reproductive immunology (New York, N.Y. : 1989). 2023; 90(2):e13743. PM ID: 37491917
-
Xu, W, et al. (2023) Exosomal PIK3CB promotes PD-L1 expression and malignant transformation in esophageal squamous cell carcinoma. Medical oncology (Northwood, London, England). 2023; 40(8):221. PM ID: 37402056
-
Zhang, R, et al. (2022) LncRNA SNHG1 promotes sepsis-induced myocardial injury by inhibiting Bcl-2 expression via DNMT1. Journal of cellular and molecular medicine. 2022;. PM ID: 35678255
-
Liu, HC, Zhu, WY & Ren, LY. (2022) LncRNA H19 inhibits proliferation and enhances apoptosis of nephroblastoma cells by regulating the miR-675/TGFBI axis. European review for medical and pharmacological sciences. 2022; 26(11):3800-3806. PM ID: 35731049
-
Agit, B. (2022) Endogenous retroviral proteins as potential drug targets for merlin-deficient tumours. Thesis. 2022;. Link: Thesis
-
Zhu, X, et al. (2022) DNMT3B-mediated FAM111B methylation promotes papillary thyroid tumor glycolysis, growth and metastasis. ijbs.com. 2022;. Link: ijbs.com
-
Wang, Q, et al. (2022) Fbxo45-mediated NP-STEP46 degradation via K6-linked ubiquitination sustains ERK activity in lung cancer. Molecular oncology. 2022;. PM ID: 35838331
-
Ye, Q, et al. (2022) Let-7b-5p inhibits breast cancer cell growth and metastasis via repression of hexokinase 2-mediated aerobic glycolysis. Research Square. 2022;. Link: Research Square
-
Zhang, M, et al. (2022) Endothelial cells regulated by RNF20 orchestrate the proliferation and differentiation of neural precursor cells during embryonic development. Cell reports. 2022; 40(11):111350. PM ID: 36103829
-
Athans, S, et al. (2022) STAG2 expression is associated with adverse survival outcomes and regulates cell phenotype in muscle-invasive bladder cancer. Cancer research communications. 2022; 2(10):1129-1143. PM ID: 36275363
- See More
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| SI506-000PA-1 | pGreenPuro Scramble Hairpin Control - Construct (EF1) | 10 µg | $594 |
|
||||
Overview
Overview
Set up stable, heritable RNAi
Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors also efficiently deliver RNAi. Generate cell lines with stable, heritable gene silencing to develop a throrough understand of the target gene’s function. Our HIV-based pGreenPuro (EF1α) shRNA Cloning and Expression Lentivector shRNA Cloning and Expression Lentivector drives expression of your shRNA template from the H1 promoter, and after processing in the cell, your shRNA will be converted into siRNA. The vector also co-expresses puromycin and copGFP from the moderate EF1α promoter, with co-expression mediated by a T2A element.
How It Works
How It Works
Using SBI’s shRNA lentivectors to produce siRNAs
To produce siRNAs for RNAi using the pSIF1-H1-H2Kk Cloning and Expression Lentivector, first clone your shRNA template into the unique BamHI or EcoRI sites in the vector. After packaging and transduction, the vector will integrate into the genome and your shRNA will be transcribed from the H1 promoter using RNA polymerase III. The shRNA is transcribed as a single strand with a sense-loop-anti-sense structure that folds into a hairpin, and is then processed by DICER to produce an active siRNA molecule (Figure 1).
Figure 1. Generating siRNA from the pGreenPuro (EF1α) shRNA Cloning and Expression Lentivector.
Supporting Data
Supporting Data
Using SBI’s shRNA lentivectors—selecting for transductants
Figure 2. Using SBI’s shRNA lentivectors. Easily select transductants with one of our markers—these examples show selection using GFP and puromycin markers on either the pSIH1-H1-copGFP (Cat.# SI501B-1) or pGreenPuro™ (Cat.# SI505A-1VB-1) shRNA Cloning and Expression Lentivectors.
FAQs
Citations
-
Zeng, ZC, et al. (2023) METTL3 protects METTL14 from STUB1-mediated degradation to maintain m6 A homeostasis. EMBO reports. 2023;:e55762. PM ID: 36597993
-
Sierra-Magro, A, et al. (2023) C/EBPβ Regulates TFAM Expression, Mitochondrial Function and Autophagy in Cellular Models of Parkinson’s Disease. International journal of molecular sciences. 2023; 24(2). PM ID: 36674978
-
Ma, J, et al. (2023) Enhanced E6AP-mediated ubiquitination of ENO1 via LINC00663 contributes to radiosensitivity of breast cancer by regulating mitochondrial homeostasis. Cancer Letters. 2023;:216118. Link: Cancer Letters
-
Wen, J, et al. (2023) Lnc-17Rik promotes the immunosuppressive function of Myeloid-Derived suppressive cells in esophageal cancer. Cellular immunology. 2023; 385:104676. PM ID: 36780770
-
Hasegawa, S, Imai, M & Takahashi, N. (2023) Role of acetoacetyl-CoA synthetase in glucose uptake by HepG2 cells. bioRxiv. 2023;. Link: bioRxiv
-
Agarwal, S, et al. (2023) BZW2 Inhibition Reduces Colorectal Cancer Growth and Metastasis. Molecular cancer research : MCR. 2023;:OF1-OF15. PM ID: 37067340
-
Song, S, et al. (2023) CHMP4A stimulates CD8+ T-lymphocyte infiltration and inhibits breast tumor growth via the LSD1/IFNβ axis. Cancer science. 2023;. PM ID: 37198999
-
Shaker, BT, et al. (2023) The 14-Kilodalton Human Growth Hormone Fragment a Potent Inhibitor of Angiogenesis and Tumor Metastasis. International journal of molecular sciences. 2023; 24(10). PM ID: 37240223
-
Ju, Z, et al. (2023) TXNL4B regulates radioresistance by controlling the PRP3-mediated alternative splicing of FANCI. MedComm. 2023; 4(3):e258. PM ID: 37168687
-
Salehi, S, et al. (2023) Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr. Nature communications. 2023; 14(1):4158. PM ID: 37438340
-
Zhang, B, et al. (2023) WIF1 promoter hypermethylation induce endometrial carcinogenesis through the Wnt/β-catenin signaling pathway. American journal of reproductive immunology (New York, N.Y. : 1989). 2023; 90(2):e13743. PM ID: 37491917
-
Xu, W, et al. (2023) Exosomal PIK3CB promotes PD-L1 expression and malignant transformation in esophageal squamous cell carcinoma. Medical oncology (Northwood, London, England). 2023; 40(8):221. PM ID: 37402056
-
Zhang, R, et al. (2022) LncRNA SNHG1 promotes sepsis-induced myocardial injury by inhibiting Bcl-2 expression via DNMT1. Journal of cellular and molecular medicine. 2022;. PM ID: 35678255
-
Liu, HC, Zhu, WY & Ren, LY. (2022) LncRNA H19 inhibits proliferation and enhances apoptosis of nephroblastoma cells by regulating the miR-675/TGFBI axis. European review for medical and pharmacological sciences. 2022; 26(11):3800-3806. PM ID: 35731049
-
Agit, B. (2022) Endogenous retroviral proteins as potential drug targets for merlin-deficient tumours. Thesis. 2022;. Link: Thesis
-
Zhu, X, et al. (2022) DNMT3B-mediated FAM111B methylation promotes papillary thyroid tumor glycolysis, growth and metastasis. ijbs.com. 2022;. Link: ijbs.com
-
Wang, Q, et al. (2022) Fbxo45-mediated NP-STEP46 degradation via K6-linked ubiquitination sustains ERK activity in lung cancer. Molecular oncology. 2022;. PM ID: 35838331
-
Ye, Q, et al. (2022) Let-7b-5p inhibits breast cancer cell growth and metastasis via repression of hexokinase 2-mediated aerobic glycolysis. Research Square. 2022;. Link: Research Square
-
Zhang, M, et al. (2022) Endothelial cells regulated by RNF20 orchestrate the proliferation and differentiation of neural precursor cells during embryonic development. Cell reports. 2022; 40(11):111350. PM ID: 36103829
-
Athans, S, et al. (2022) STAG2 expression is associated with adverse survival outcomes and regulates cell phenotype in muscle-invasive bladder cancer. Cancer research communications. 2022; 2(10):1129-1143. PM ID: 36275363
- See More