pGreenFire1-Notch Lentivector

Study Notch signal transduction by co-expressing dscGFP and luciferase in response to Notch activity
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

Products

Catalog Number Description Size Price Quantity Add to Cart
TR020PA-1 pGreenFire1-Notch (plasmid) 10 µg $691.00
- +
Contact Us
TR020VA-1 pGreenFire1-Notch (virus) >2 x 10^6 IFUs $691.00
- +
Contact Us

Overview

Overview

Monitor signal transduction in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-Notch Lentivector co-expresses a destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase from Notch transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of Notch-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to Notch activity. The result is the ability to quantitatively measure Notch activity by fluorescence and luciferase activity.
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGreenFire1-Notch Lentivector To facilitate cell line construction, the pGreenFire1-Notch Lentivector also comes with a constitutively-expressed neomycin marker (EF1α-neo; Cat.# TR020PA-N) or puromycin marker (EF1α-neo; Cat.# TR020PA-P). In addition, all forms of this vector are available as both lentivector plasmid, and pre-packaged virus. *Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

Resources

Citations

We're sorry, an error has occurred while generating this content.

Products

Catalog Number Description Size Price Quantity Add to Cart
TR020PA-1 pGreenFire1-Notch (plasmid) 10 µg $691.00
- +
Contact Us
TR020VA-1 pGreenFire1-Notch (virus) >2 x 10^6 IFUs $691.00
- +
Contact Us

Overview

Overview

Monitor signal transduction in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-Notch Lentivector co-expresses a destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase from Notch transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of Notch-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to Notch activity. The result is the ability to quantitatively measure Notch activity by fluorescence and luciferase activity.
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGreenFire1-Notch Lentivector To facilitate cell line construction, the pGreenFire1-Notch Lentivector also comes with a constitutively-expressed neomycin marker (EF1α-neo; Cat.# TR020PA-N) or puromycin marker (EF1α-neo; Cat.# TR020PA-P). In addition, all forms of this vector are available as both lentivector plasmid, and pre-packaged virus. *Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

Citations

We're sorry, an error has occurred while generating this content.

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