pGreenFire1-NF-κB Lentivector

Study NF-κB signal transduction by co-expressing dscGFP and luciferase in response to NF-κB activity
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

Products

Catalog Number Description Size Price Quantity Add to Cart
TR012PA-1 pGreenFire1-NF-kB (plasmid) 10 µg $706
- +
TR012VA-1 pGreenFire1-NF-kB (virus) >2 x 10^6 IFUs $706
- +

Overview

Overview

Monitor signal transduction in real time

With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-NF-κB Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from NF-κB transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of NF-κB-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to NF-κB activity. The result is the ability to quantitatively measure NF-κB activity by fluorescence and luciferase activity.

  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGreenFire1-NF-κB Lentivector

To facilitate cell line construction, the pGreenFire1-NF-κB Lentivector also comes with a constitutively-expressed neomycin marker (EF1α-neo; Cat.# TR012PA-N) or puromycin marker (EF1α-neo; Cat.# TR012PA-P). In addition, all forms of this vector are available as both lentivector plasmid, and pre-packaged virus.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

Resources

Citations

  • Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 1970 Jan 1;. PM ID: 35483375
  • Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 1970 Jan 1;. Link: slas-discovery.org
  • Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 1970 Jan 1; 21(6):710-720. PM ID: 35606429
  • Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 1970 Jan 1;. Link: Research Square
  • Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 1970 Jan 1; 11(7). PM ID: 35884187
  • Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 1970 Jan 1; 25:101508. PM ID: 35985204
  • Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 1970 Jan 1;. PM ID: 33545065
  • Deng, P, et al. (2021) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell stem cell. 1970 Jan 1;. PM ID: 33571444
  • Bencomo-Alvarez, AE, et al. (2021) Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B. Oncogene. 1970 Jan 1;. PM ID: 33712704
  • Miyashita, Y, et al. (2021) The CR9 element is a novel mechanical load-responsive enhancer that regulates natriuretic peptide genes expression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 1970 Jan 1; 35(4):e21495. PM ID: 33689182
  • Sugali, CK, et al. (2021) Full title: The canonical Wnt signaling pathway inhibits the glucocorticoid receptor signaling pathway in the trabecular meshwork. The American journal of pathology. 1970 Jan 1;. PM ID: 33705750
  • Cook, B, et al. (2021) The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3 β signaling. The Journal of biological chemistry. 1970 Jan 1;:100696. PM ID: 33895139
  • Slominski, AT, et al. (2021) Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs). Scientific reports. 1970 Jan 1; 11(1):8002. PM ID: 33850196
  • Jang, HJ, et al. (2021) Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells. Integrative cancer therapies. 1970 Jan 1; 20:15347354211006191. PM ID: 33884928
  • Gavini, C, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin-1 Expression and Sciatic Nerve-Associated Cell signaling. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Li, X, et al. (2020) Programmable base editing of mutated TERT promoter inhibits brain tumour growth. Nat. Cell Biol.. 1970 Jan 1;. PM ID: 32066906
  • Blaževitš, O, et al. (2020) MYC-Associated Factor MAX is a Regulator of the Circadian Clock. Int J Mol Sci. 1970 Jan 1; 21(7). PM ID: 32225100
  • Jeong, EM, et al. (2020) Competitive Binding of Magnesium to Calcium Binding Sites Reciprocally Regulates Transamidase and GTP Hydrolysis Activity of Transglutaminase 2. Int J Mol Sci. 1970 Jan 1; 21(3). PM ID: 31991788
  • Gavini, CK, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin 1 Expression and Sciatic Nerve-Associated Cell Signaling in Western Diet-fed Rodents. Sci Rep. 1970 Jan 1; 10(1):6396. PM ID: 32286429
  • Tummala, H, et al. (2020) A frameshift variant in specificity protein 1 triggers superactivation of Sp1-mediated transcription in familial bone marrow failure. Proc. Natl. Acad. Sci. U.S.A.. 1970 Jan 1; 117(29):17151-17155. Link: Proc. Natl. Acad. Sci. U.S.A.

Products

Catalog Number Description Size Price Quantity Add to Cart
TR012PA-1 pGreenFire1-NF-kB (plasmid) 10 µg $706
- +
TR012VA-1 pGreenFire1-NF-kB (virus) >2 x 10^6 IFUs $706
- +

Overview

Overview

Monitor signal transduction in real time

With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-NF-κB Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from NF-κB transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of NF-κB-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to NF-κB activity. The result is the ability to quantitatively measure NF-κB activity by fluorescence and luciferase activity.

  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGreenFire1-NF-κB Lentivector

To facilitate cell line construction, the pGreenFire1-NF-κB Lentivector also comes with a constitutively-expressed neomycin marker (EF1α-neo; Cat.# TR012PA-N) or puromycin marker (EF1α-neo; Cat.# TR012PA-P). In addition, all forms of this vector are available as both lentivector plasmid, and pre-packaged virus.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

Citations

  • Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 1970 Jan 1;. PM ID: 35483375
  • Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 1970 Jan 1;. Link: slas-discovery.org
  • Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 1970 Jan 1; 21(6):710-720. PM ID: 35606429
  • Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 1970 Jan 1;. Link: Research Square
  • Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 1970 Jan 1; 11(7). PM ID: 35884187
  • Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 1970 Jan 1; 25:101508. PM ID: 35985204
  • Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 1970 Jan 1;. PM ID: 33545065
  • Deng, P, et al. (2021) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell stem cell. 1970 Jan 1;. PM ID: 33571444
  • Bencomo-Alvarez, AE, et al. (2021) Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B. Oncogene. 1970 Jan 1;. PM ID: 33712704
  • Miyashita, Y, et al. (2021) The CR9 element is a novel mechanical load-responsive enhancer that regulates natriuretic peptide genes expression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 1970 Jan 1; 35(4):e21495. PM ID: 33689182
  • Sugali, CK, et al. (2021) Full title: The canonical Wnt signaling pathway inhibits the glucocorticoid receptor signaling pathway in the trabecular meshwork. The American journal of pathology. 1970 Jan 1;. PM ID: 33705750
  • Cook, B, et al. (2021) The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3 β signaling. The Journal of biological chemistry. 1970 Jan 1;:100696. PM ID: 33895139
  • Slominski, AT, et al. (2021) Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs). Scientific reports. 1970 Jan 1; 11(1):8002. PM ID: 33850196
  • Jang, HJ, et al. (2021) Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells. Integrative cancer therapies. 1970 Jan 1; 20:15347354211006191. PM ID: 33884928
  • Gavini, C, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin-1 Expression and Sciatic Nerve-Associated Cell signaling. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Li, X, et al. (2020) Programmable base editing of mutated TERT promoter inhibits brain tumour growth. Nat. Cell Biol.. 1970 Jan 1;. PM ID: 32066906
  • Blaževitš, O, et al. (2020) MYC-Associated Factor MAX is a Regulator of the Circadian Clock. Int J Mol Sci. 1970 Jan 1; 21(7). PM ID: 32225100
  • Jeong, EM, et al. (2020) Competitive Binding of Magnesium to Calcium Binding Sites Reciprocally Regulates Transamidase and GTP Hydrolysis Activity of Transglutaminase 2. Int J Mol Sci. 1970 Jan 1; 21(3). PM ID: 31991788
  • Gavini, CK, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin 1 Expression and Sciatic Nerve-Associated Cell Signaling in Western Diet-fed Rodents. Sci Rep. 1970 Jan 1; 10(1):6396. PM ID: 32286429
  • Tummala, H, et al. (2020) A frameshift variant in specificity protein 1 triggers superactivation of Sp1-mediated transcription in familial bone marrow failure. Proc. Natl. Acad. Sci. U.S.A.. 1970 Jan 1; 117(29):17151-17155. Link: Proc. Natl. Acad. Sci. U.S.A.