pGreenFire1-Gal4 (EF1α-puro) Lentivector

Study Gal4 signal transduction by co-expressing dscGFP and luciferase in response to Gal4 activity (includes EF1α-puro selection cassette)
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

Products

Catalog Number Description Size Price Quantity Add to Cart
TR017PA-P pGreenFire1-Gal4 (plasmid)+ EF1α-Puro 10 µg $691.00
- +
Contact Us
TR017VA-P pGreenFire1-Gal4 (virus) + EF1α-Puro >2 x 10^6 IFUs $691.00
- +
Contact Us

Overview

Overview

Monitor signal transduction in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-Gal4 (EF1α-puro) Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from Gal4 transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of Gal4-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to Gal4 activity. The result is the ability to quantitatively measure Gal4 activity by fluorescence and luciferase activity.
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGreenFire1-Gal4 (EF1α-puro) Lentivector

To facilitate cell line construction, the pGreenFire1-Gal4 (EF1α-puro) Lentivector comes with a constitutively-expressed puromycin marker from the EF1α promoter. Or you can choose a neomycin marker (EF1α-neo; Cat.# TR017PA-N) or no marker at all (Cat.# TR017PA-1). In addition, all forms of this vector are available as both lentivector plasmid, and pre-packaged virus.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

Resources

Citations

  • Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 1970 Jan 1;. PM ID: 33545065
  • Deng, P, et al. (2021) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell stem cell. 1970 Jan 1;. PM ID: 33571444
  • Bencomo-Alvarez, AE, et al. (2021) Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B. Oncogene. 1970 Jan 1;. PM ID: 33712704
  • Miyashita, Y, et al. (2021) The CR9 element is a novel mechanical load-responsive enhancer that regulates natriuretic peptide genes expression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 1970 Jan 1; 35(4):e21495. PM ID: 33689182
  • Sugali, CK, et al. (2021) Full title: The canonical Wnt signaling pathway inhibits the glucocorticoid receptor signaling pathway in the trabecular meshwork. The American journal of pathology. 1970 Jan 1;. PM ID: 33705750
  • Cook, B, et al. (2021) The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3 β signaling. The Journal of biological chemistry. 1970 Jan 1;:100696. PM ID: 33895139
  • Slominski, AT, et al. (2021) Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs). Scientific reports. 1970 Jan 1; 11(1):8002. PM ID: 33850196
  • Jang, HJ, et al. (2021) Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells. Integrative cancer therapies. 1970 Jan 1; 20:15347354211006191. PM ID: 33884928
  • Gavini, C, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin-1 Expression and Sciatic Nerve-Associated Cell signaling. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Li, X, et al. (2020) Programmable base editing of mutated TERT promoter inhibits brain tumour growth. Nat. Cell Biol.. 1970 Jan 1;. PM ID: 32066906
  • Blaževitš, O, et al. (2020) MYC-Associated Factor MAX is a Regulator of the Circadian Clock. Int J Mol Sci. 1970 Jan 1; 21(7). PM ID: 32225100
  • Jeong, EM, et al. (2020) Competitive Binding of Magnesium to Calcium Binding Sites Reciprocally Regulates Transamidase and GTP Hydrolysis Activity of Transglutaminase 2. Int J Mol Sci. 1970 Jan 1; 21(3). PM ID: 31991788
  • Gavini, CK, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin 1 Expression and Sciatic Nerve-Associated Cell Signaling in Western Diet-fed Rodents. Sci Rep. 1970 Jan 1; 10(1):6396. PM ID: 32286429
  • Tummala, H, et al. (2020) A frameshift variant in specificity protein 1 triggers superactivation of Sp1-mediated transcription in familial bone marrow failure. Proc. Natl. Acad. Sci. U.S.A.. 1970 Jan 1; 117(29):17151-17155. Link: Proc. Natl. Acad. Sci. U.S.A.
  • Abraham, J, et al. (2020) Characterization of a Novel Compound That Stimulates STING-Mediated Innate Immune Activity in an Allele-Specific Manner. Front Immunol. 1970 Jan 1; 11:1430. PM ID: 32733475
  • Paramathas, S. (2020) Investigating the use of circulating tumor DNA for cancer surveillance and early detection in (pediatric) sarcomas and Li-Fraumeni Syndrome. Thesis. 1970 Jan 1;. Link: Thesis
  • Hayashi, Y, et al. (2020) Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion During Aging. Cell Mol Gastroenterol Hepatol. 1970 Jan 1;. PM ID: 32771388
  • Chandra Boinpelly, V, et al. (2020) α-Mangostin-encapsulated PLGA nanoparticles inhibit colorectal cancer growth by inhibiting Notch pathway. J. Cell. Mol. Med.. 1970 Jan 1;. PM ID: 32830433
  • Fearing, B, et al. (2020) Verteporfin treatment controls morphology, phenotype, and global gene expression for cells of the human nucleus pulposus. JOR Spine. 1970 Jan 1;:e1111. Link: JOR Spine
  • Lukonin, I, et al. (2020) Phenotypic landscape of intestinal organoid regeneration. Nature. 1970 Jan 1; 586(7828):275-280. PM ID: 33029001

Products

Catalog Number Description Size Price Quantity Add to Cart
TR017PA-P pGreenFire1-Gal4 (plasmid)+ EF1α-Puro 10 µg $691.00
- +
Contact Us
TR017VA-P pGreenFire1-Gal4 (virus) + EF1α-Puro >2 x 10^6 IFUs $691.00
- +
Contact Us

Overview

Overview

Monitor signal transduction in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-Gal4 (EF1α-puro) Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from Gal4 transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of Gal4-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to Gal4 activity. The result is the ability to quantitatively measure Gal4 activity by fluorescence and luciferase activity.
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGreenFire1-Gal4 (EF1α-puro) Lentivector

To facilitate cell line construction, the pGreenFire1-Gal4 (EF1α-puro) Lentivector comes with a constitutively-expressed puromycin marker from the EF1α promoter. Or you can choose a neomycin marker (EF1α-neo; Cat.# TR017PA-N) or no marker at all (Cat.# TR017PA-1). In addition, all forms of this vector are available as both lentivector plasmid, and pre-packaged virus.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

Citations

  • Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 1970 Jan 1;. PM ID: 33545065
  • Deng, P, et al. (2021) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell stem cell. 1970 Jan 1;. PM ID: 33571444
  • Bencomo-Alvarez, AE, et al. (2021) Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B. Oncogene. 1970 Jan 1;. PM ID: 33712704
  • Miyashita, Y, et al. (2021) The CR9 element is a novel mechanical load-responsive enhancer that regulates natriuretic peptide genes expression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 1970 Jan 1; 35(4):e21495. PM ID: 33689182
  • Sugali, CK, et al. (2021) Full title: The canonical Wnt signaling pathway inhibits the glucocorticoid receptor signaling pathway in the trabecular meshwork. The American journal of pathology. 1970 Jan 1;. PM ID: 33705750
  • Cook, B, et al. (2021) The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3 β signaling. The Journal of biological chemistry. 1970 Jan 1;:100696. PM ID: 33895139
  • Slominski, AT, et al. (2021) Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs). Scientific reports. 1970 Jan 1; 11(1):8002. PM ID: 33850196
  • Jang, HJ, et al. (2021) Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells. Integrative cancer therapies. 1970 Jan 1; 20:15347354211006191. PM ID: 33884928
  • Gavini, C, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin-1 Expression and Sciatic Nerve-Associated Cell signaling. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Li, X, et al. (2020) Programmable base editing of mutated TERT promoter inhibits brain tumour growth. Nat. Cell Biol.. 1970 Jan 1;. PM ID: 32066906
  • Blaževitš, O, et al. (2020) MYC-Associated Factor MAX is a Regulator of the Circadian Clock. Int J Mol Sci. 1970 Jan 1; 21(7). PM ID: 32225100
  • Jeong, EM, et al. (2020) Competitive Binding of Magnesium to Calcium Binding Sites Reciprocally Regulates Transamidase and GTP Hydrolysis Activity of Transglutaminase 2. Int J Mol Sci. 1970 Jan 1; 21(3). PM ID: 31991788
  • Gavini, CK, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin 1 Expression and Sciatic Nerve-Associated Cell Signaling in Western Diet-fed Rodents. Sci Rep. 1970 Jan 1; 10(1):6396. PM ID: 32286429
  • Tummala, H, et al. (2020) A frameshift variant in specificity protein 1 triggers superactivation of Sp1-mediated transcription in familial bone marrow failure. Proc. Natl. Acad. Sci. U.S.A.. 1970 Jan 1; 117(29):17151-17155. Link: Proc. Natl. Acad. Sci. U.S.A.
  • Abraham, J, et al. (2020) Characterization of a Novel Compound That Stimulates STING-Mediated Innate Immune Activity in an Allele-Specific Manner. Front Immunol. 1970 Jan 1; 11:1430. PM ID: 32733475
  • Paramathas, S. (2020) Investigating the use of circulating tumor DNA for cancer surveillance and early detection in (pediatric) sarcomas and Li-Fraumeni Syndrome. Thesis. 1970 Jan 1;. Link: Thesis
  • Hayashi, Y, et al. (2020) Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion During Aging. Cell Mol Gastroenterol Hepatol. 1970 Jan 1;. PM ID: 32771388
  • Chandra Boinpelly, V, et al. (2020) α-Mangostin-encapsulated PLGA nanoparticles inhibit colorectal cancer growth by inhibiting Notch pathway. J. Cell. Mol. Med.. 1970 Jan 1;. PM ID: 32830433
  • Fearing, B, et al. (2020) Verteporfin treatment controls morphology, phenotype, and global gene expression for cells of the human nucleus pulposus. JOR Spine. 1970 Jan 1;:e1111. Link: JOR Spine
  • Lukonin, I, et al. (2020) Phenotypic landscape of intestinal organoid regeneration. Nature. 1970 Jan 1; 586(7828):275-280. PM ID: 33029001