pGF-EGR1-mCMV-EF1α-Puro Lentivector

Study oncogenic signaling by co-expressing dscGFP and luciferase in response to EGR1 activity at EGR1 transcriptional response elements
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

Products

Catalog Number Description Size Price Quantity Add to Cart
TR204a-p pGF-EGR1-mCMV-EF1α-Puro (293 stable cell line) >2 x 10^6 Cells $3,014.00
- +
Contact Us
TR204pa-p pGF-EGR1-mCMV-EF1α-Puro (plasmid) 10 µg $691.00
- +
Contact Us
TR204va-p pGF-EGR1-mCMV-EF1α-Puro (virus) >2 x 10^6 IFUs $691.00
- +
Contact Us

Overview

Overview

Monitor oncogenic signaling in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGF-EGR1-mCMV-EF1α-Puro Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from EGR1 transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of EGR1 TREs, drives expression of dscGFP and luciferase in response to EGR1 activity. The result is the ability to quantitatively measure EGR1 activity at EGR1 TREs by fluorescence and luciferase activity.
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGF-EGR1-mCMV-EF1α-Puro LentivectorThe pGF-EGR1-mCMV-EF1α-Puro Lentivector is available as a lentivector, pre-packaged virus, and a stable cell line created from the pGF-EGR1-mCMV-EF1α-Puro Lentivector transduced into 293 cells. *Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cells

Track and measure the activity of oncogenic signal transduction pathways in live cells

 

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

 

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

 

General pGreenFire data examples

See SBI’s pGreenFire1 reporters in action

 

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in action

 

See SBI’s pGreenFire1 reporters in action

Resources

Citations

  • Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 1970 Jan 1;. PM ID: 33545065
  • Deng, P, et al. (2021) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell stem cell. 1970 Jan 1;. PM ID: 33571444
  • Bencomo-Alvarez, AE, et al. (2021) Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B. Oncogene. 1970 Jan 1;. PM ID: 33712704
  • Miyashita, Y, et al. (2021) The CR9 element is a novel mechanical load-responsive enhancer that regulates natriuretic peptide genes expression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 1970 Jan 1; 35(4):e21495. PM ID: 33689182
  • Sugali, CK, et al. (2021) Full title: The canonical Wnt signaling pathway inhibits the glucocorticoid receptor signaling pathway in the trabecular meshwork. The American journal of pathology. 1970 Jan 1;. PM ID: 33705750
  • Cook, B, et al. (2021) The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3 β signaling. The Journal of biological chemistry. 1970 Jan 1;:100696. PM ID: 33895139
  • Slominski, AT, et al. (2021) Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs). Scientific reports. 1970 Jan 1; 11(1):8002. PM ID: 33850196
  • Jang, HJ, et al. (2021) Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells. Integrative cancer therapies. 1970 Jan 1; 20:15347354211006191. PM ID: 33884928
  • Gavini, C, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin-1 Expression and Sciatic Nerve-Associated Cell signaling. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Li, X, et al. (2020) Programmable base editing of mutated TERT promoter inhibits brain tumour growth. Nat. Cell Biol.. 1970 Jan 1;. PM ID: 32066906
  • Blaževitš, O, et al. (2020) MYC-Associated Factor MAX is a Regulator of the Circadian Clock. Int J Mol Sci. 1970 Jan 1; 21(7). PM ID: 32225100
  • Jeong, EM, et al. (2020) Competitive Binding of Magnesium to Calcium Binding Sites Reciprocally Regulates Transamidase and GTP Hydrolysis Activity of Transglutaminase 2. Int J Mol Sci. 1970 Jan 1; 21(3). PM ID: 31991788
  • Gavini, CK, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin 1 Expression and Sciatic Nerve-Associated Cell Signaling in Western Diet-fed Rodents. Sci Rep. 1970 Jan 1; 10(1):6396. PM ID: 32286429
  • Tummala, H, et al. (2020) A frameshift variant in specificity protein 1 triggers superactivation of Sp1-mediated transcription in familial bone marrow failure. Proc. Natl. Acad. Sci. U.S.A.. 1970 Jan 1; 117(29):17151-17155. Link: Proc. Natl. Acad. Sci. U.S.A.
  • Abraham, J, et al. (2020) Characterization of a Novel Compound That Stimulates STING-Mediated Innate Immune Activity in an Allele-Specific Manner. Front Immunol. 1970 Jan 1; 11:1430. PM ID: 32733475
  • Paramathas, S. (2020) Investigating the use of circulating tumor DNA for cancer surveillance and early detection in (pediatric) sarcomas and Li-Fraumeni Syndrome. Thesis. 1970 Jan 1;. Link: Thesis
  • Hayashi, Y, et al. (2020) Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion During Aging. Cell Mol Gastroenterol Hepatol. 1970 Jan 1;. PM ID: 32771388
  • Chandra Boinpelly, V, et al. (2020) α-Mangostin-encapsulated PLGA nanoparticles inhibit colorectal cancer growth by inhibiting Notch pathway. J. Cell. Mol. Med.. 1970 Jan 1;. PM ID: 32830433
  • Fearing, B, et al. (2020) Verteporfin treatment controls morphology, phenotype, and global gene expression for cells of the human nucleus pulposus. JOR Spine. 1970 Jan 1;:e1111. Link: JOR Spine
  • Lukonin, I, et al. (2020) Phenotypic landscape of intestinal organoid regeneration. Nature. 1970 Jan 1; 586(7828):275-280. PM ID: 33029001

Products

Catalog Number Description Size Price Quantity Add to Cart
TR204a-p pGF-EGR1-mCMV-EF1α-Puro (293 stable cell line) >2 x 10^6 Cells $3,014.00
- +
Contact Us
TR204pa-p pGF-EGR1-mCMV-EF1α-Puro (plasmid) 10 µg $691.00
- +
Contact Us
TR204va-p pGF-EGR1-mCMV-EF1α-Puro (virus) >2 x 10^6 IFUs $691.00
- +
Contact Us

Overview

Overview

Monitor oncogenic signaling in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGF-EGR1-mCMV-EF1α-Puro Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from EGR1 transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of EGR1 TREs, drives expression of dscGFP and luciferase in response to EGR1 activity. The result is the ability to quantitatively measure EGR1 activity at EGR1 TREs by fluorescence and luciferase activity.
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGF-EGR1-mCMV-EF1α-Puro LentivectorThe pGF-EGR1-mCMV-EF1α-Puro Lentivector is available as a lentivector, pre-packaged virus, and a stable cell line created from the pGF-EGR1-mCMV-EF1α-Puro Lentivector transduced into 293 cells. *Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cells

Track and measure the activity of oncogenic signal transduction pathways in live cells

 

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

 

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

 

General pGreenFire data examples

See SBI’s pGreenFire1 reporters in action

 

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in action

 

See SBI’s pGreenFire1 reporters in action

Citations

  • Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 1970 Jan 1;. PM ID: 33545065
  • Deng, P, et al. (2021) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell stem cell. 1970 Jan 1;. PM ID: 33571444
  • Bencomo-Alvarez, AE, et al. (2021) Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B. Oncogene. 1970 Jan 1;. PM ID: 33712704
  • Miyashita, Y, et al. (2021) The CR9 element is a novel mechanical load-responsive enhancer that regulates natriuretic peptide genes expression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 1970 Jan 1; 35(4):e21495. PM ID: 33689182
  • Sugali, CK, et al. (2021) Full title: The canonical Wnt signaling pathway inhibits the glucocorticoid receptor signaling pathway in the trabecular meshwork. The American journal of pathology. 1970 Jan 1;. PM ID: 33705750
  • Cook, B, et al. (2021) The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3 β signaling. The Journal of biological chemistry. 1970 Jan 1;:100696. PM ID: 33895139
  • Slominski, AT, et al. (2021) Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs). Scientific reports. 1970 Jan 1; 11(1):8002. PM ID: 33850196
  • Jang, HJ, et al. (2021) Chelidonine Induces Apoptosis via GADD45a-p53 Regulation in Human Pancreatic Cancer Cells. Integrative cancer therapies. 1970 Jan 1; 20:15347354211006191. PM ID: 33884928
  • Gavini, C, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin-1 Expression and Sciatic Nerve-Associated Cell signaling. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Li, X, et al. (2020) Programmable base editing of mutated TERT promoter inhibits brain tumour growth. Nat. Cell Biol.. 1970 Jan 1;. PM ID: 32066906
  • Blaževitš, O, et al. (2020) MYC-Associated Factor MAX is a Regulator of the Circadian Clock. Int J Mol Sci. 1970 Jan 1; 21(7). PM ID: 32225100
  • Jeong, EM, et al. (2020) Competitive Binding of Magnesium to Calcium Binding Sites Reciprocally Regulates Transamidase and GTP Hydrolysis Activity of Transglutaminase 2. Int J Mol Sci. 1970 Jan 1; 21(3). PM ID: 31991788
  • Gavini, CK, Bonomo, R & Mansuy-Aubert, V. (2020) Neuronal LXR Regulates Neuregulin 1 Expression and Sciatic Nerve-Associated Cell Signaling in Western Diet-fed Rodents. Sci Rep. 1970 Jan 1; 10(1):6396. PM ID: 32286429
  • Tummala, H, et al. (2020) A frameshift variant in specificity protein 1 triggers superactivation of Sp1-mediated transcription in familial bone marrow failure. Proc. Natl. Acad. Sci. U.S.A.. 1970 Jan 1; 117(29):17151-17155. Link: Proc. Natl. Acad. Sci. U.S.A.
  • Abraham, J, et al. (2020) Characterization of a Novel Compound That Stimulates STING-Mediated Innate Immune Activity in an Allele-Specific Manner. Front Immunol. 1970 Jan 1; 11:1430. PM ID: 32733475
  • Paramathas, S. (2020) Investigating the use of circulating tumor DNA for cancer surveillance and early detection in (pediatric) sarcomas and Li-Fraumeni Syndrome. Thesis. 1970 Jan 1;. Link: Thesis
  • Hayashi, Y, et al. (2020) Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion During Aging. Cell Mol Gastroenterol Hepatol. 1970 Jan 1;. PM ID: 32771388
  • Chandra Boinpelly, V, et al. (2020) α-Mangostin-encapsulated PLGA nanoparticles inhibit colorectal cancer growth by inhibiting Notch pathway. J. Cell. Mol. Med.. 1970 Jan 1;. PM ID: 32830433
  • Fearing, B, et al. (2020) Verteporfin treatment controls morphology, phenotype, and global gene expression for cells of the human nucleus pulposus. JOR Spine. 1970 Jan 1;:e1111. Link: JOR Spine
  • Lukonin, I, et al. (2020) Phenotypic landscape of intestinal organoid regeneration. Nature. 1970 Jan 1; 586(7828):275-280. PM ID: 33029001