PEG-it Virus Precipitation Solution

Easily concentrate lentivirus with an ultracentrifugation-free protocol—great for scaled-up virus production and also protects virus from freeze-thaw cycles

Products

Catalog Number Description Size Price Quantity Add to Cart
LV810A-1 PEG-it Virus Precipitation Solution 100 mL $337
- +
LV825A-1 PEG-it Virus Precipitation Solution 250 mL $737
- +

Overview

Overview

Easily concentrate lentivirus particles for ultra-high titers

Used in over three-hundred citations, PEG-it™ Virus Concentration Reagent enables easy concentration of pseudoviral particles for achieving ultra-high titers. It’s great for concentrating pseudoviral particles even from large volumes of medium by removing the need for ultracentrifugation. Simply add PEG-it to the collected medium, incubate overnight at 4°C, and spin at 1500g for 30 minutes.

In addition, PEG-it acts as a cryopreservative for concentrated virus. Lentivirus concentrated with PEG-it lasts longer in the freezer and survives multiple freeze-thaw cycles with minimal loss of titer.

How It Works

How It Works

PEG-it is an easy way to get higher viral titers by concentrating virus

Using PEG-it to concentrate virus particles is easy with little hands-on time

Supporting Data

Supporting Data

PEG-it concentrates virus particles 10- to 100-fold

PEG-it concentrates virus particles 10- to 100-fold

Figure 1. PEG-it concentrates virus particles 10- to 100-fold. With PEG-it, virus particles can be concentrated up to 100-fold, leading to much higher infectious units per mL.

PEG-it is non-toxic and effective with stem cellsPEG-it is non-toxic and effective with stem cells

Figure 2. PEG-it is non-toxic and effective with Stem Cells. PEG-it-treated virus is non-toxic to stem cells and results in highly effective transductions. METHODS: H9 hES cells were transduced with pGreenZeo reporter constructs containing specific promoters for CMV, mOCT4 or mNANOG. Cells were cultured for eight weeks on Matrigel-coated plates with MEF conditioned medium containing 1 µg/mL Zeocin. The cells imaged here were split and grown on MEF feeder layers for four days. Data courtesy of Dr. Timothy Kamp and Chad H. Koonce, University of Wisconsin, Madison, Stem Cell and Regenerative Medicine Center.

PEG-it protects isolated virus particles from multiple freeze/thaw cyclesPEG-it protects isolated virus particles from multiple freeze/thaw cycles

Figure 3. PEG-it protects isolated virus particles from multiple freeze/thaw cycles. Lentivirus concentrated with PEG-it retains high titers even after four freeze-thaw cycles. HepG2 cells (top panel set) and HT1080 cells (bottom panel set) transduced with LV605VA-1 after 1 – 4 freeze-thaw cycles. Even after the fourth thaw, the LV605VA-1 virus particles show robust transduction efficiency.

Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective

Figure 4. Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective. The cells from Figure 3 were lysed and viral infectious units (IFUs) measured using the Global UltraRapid™ Lentiviral Titer Kit (Cat.# LV961A-1). No significant loss of IFUs due to repeated freeze/thaw cycles was found with virus particles that had been concentrated using PEG-it.

FAQs

PEG-it Virus Precipitation Solution is a reagent used to easily concentrate lentivirus particles for ultra-high titers, without the need for ultracentrifugation. It is suitable for concentrating pseudoviral particles from large volumes of medium and acts as a cryopreservative for concentrated virus.
PEG-it works by concentrating virus particles in a simple, hands-on procedure. Just add PEG-it to the collected medium, incubate overnight at 4°C, and spin at 1500g for 30 minutes. This process helps achieve higher viral titers by concentrating the virus.
PEG-it offers several benefits:
  • Concentrates virus particles 10- to 100-fold, leading to higher infectious units per mL
  • Eliminates the need for ultracentrifugation
  • Non-toxic and effective with stem cells
  • Acts as a cryopreservative, protecting the virus from multiple freeze-thaw cycles with minimal loss of titer
PEG-it acts as a cryopreservative for concentrated virus, allowing it to last longer in the freezer and survive multiple freeze-thaw cycles with minimal loss of titer.
Yes, PEG-it-treated virus is non-toxic to stem cells and results in highly effective transductions.
To speak to a specialist, you can contact System Biosciences at info@systembio.com or call our toll-free number at 1-(888) 640-4371.
PEG-it Virus Precipitation Solution is available in two sizes:
100 mL (Catalog Number: LV810A-1)
250 mL (Catalog Number: LV825A-1)
The user manual and product sheet for PEG-it Lentivirus Concentration Reagent can be found on the manufacturer's website under the "Resources" section.

Resources

Citations

  • An, M, et al. (2024) Engineered virus-like particles for transient delivery of prime editor ribonucleoprotein complexes in vivo. Nature biotechnology. 2024;. PM ID: 38191664
  • Ullah, I, et al. (2024) Bioluminescence Imaging Reveals Enhanced SARS-CoV-2 Clearance in Mice with Combinatorial Regimens. iScience. 2024;:109049. Link: iScience
  • Baudrier, L, et al. (2024) One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics. Cell reports methods. 2024;:100698. PM ID: 38301655
  • Konishi, CT, et al. (2024) Modeling and Correction of Protein Conformational Disease in iPSC-derived Neurons through Personalized Base Editing. bioRxiv : the preprint server for biology. 2024;. PM ID: 38293034
  • Botten, GA, Lee, M & Xu, J. (2023) Dissecting Locus-Specific Chromatin Interactions by CRISPR CAPTURE. Methods in molecular biology (Clifton, N.J.). 2023; 2599:69-97. PM ID: 36427144
  • Chen, Y, et al. (2023) Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD. iScience. 2023; 26(1):105783. PM ID: 36514310
  • Mlynarczyk, C, et al. (2023) BTG1 mutation yields supercompetitive B cells primed for malignant transformation. Science (New York, N.Y.). 2023; 379(6629):eabj7412. PM ID: 36656933
  • Li, X, et al. (2023) BMX controls 3βHSD1 and sex steroid biosynthesis in cancer. The Journal of clinical investigation. 2023; 133(2). PM ID: 36647826
  • Kang, H, et al. (2023) DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastoma. Cell reports. Medicine. 2023; 4(1):100880. PM ID: 36603576
  • Gurska, L, et al. (2023) Crizotinib Has Preclinical Efficacy in Philadelphia-Negative Myeloproliferative Neoplasms. Clinical Cancer Research. 2023;:OF1-OF14. Link: Clinical Cancer Research
  • Suzuki, T, et al. (2023) Pivotal Role of Ubiquitin Carboxyl-Terminal Hydrolase L1 (UCHL1) in Uterine Leiomyoma. Biomolecules. 2023; 13(2):193. Link: Biomolecules
  • Aw, WY, et al. (2023) Microphysiological model of PIK3CA-driven vascular malformations reveals a role of dysregulated Rac1 and mTORC1/2 in lesion formation. Science advances. 2023; 9(7):eade8939. PM ID: 36791204
  • Mitchell, K, et al. (2023) WDR5 represents a therapeutically exploitable target for cancer stem cells in glioblastoma. Genes & development. 2023;. PM ID: 36732025
  • Cai, Q, et al. (2023) Differential roles of CaMKII isoforms in phase separation with NMDA receptors and in synaptic plasticity. Cell reports. 2023; 42(3):112146. PM ID: 36827181
  • Li, S, et al. (2023) Dual targeting of protein translation and nuclear protein export results in enhanced anti-myeloma effects. Blood advances. 2023;. PM ID: 36827679
  • Wang, JH, et al. (2023) Development of a CRISPRi Human Retinal Pigmented Epithelium Model for Functional Study of Age-Related Macular Degeneration Genes. International journal of molecular sciences. 2023; 24(4). PM ID: 36834828
  • Ramos, E, et al. (2023) Novel Chaphamaparvovirus in Insectivorous Molossus molossus Bats, from the Brazilian Amazon Region. Viruses. 2023; 15(3):606. Link: Viruses
  • Decaesteker, B, et al. (2023) SOX11 regulates SWI/SNF complex components as member of the adrenergic neuroblastoma core regulatory circuitry. Nature communications. 2023; 14(1):1267. PM ID: 36882421
  • Cao, W, et al. (2023) TRIB2 safeguards naive T cell homeostasis during aging. Cell reports. 2023; 42(3):112195. PM ID: 36884349
  • McCann, JJ, et al. (2023) Participation of ATM, SMG1, and DDX5 in a DNA Damage-induced Alternative Splicing Pathway. Radiation research. 2023;. PM ID: 36921295

Products

Catalog Number Description Size Price Quantity Add to Cart
LV810A-1 PEG-it Virus Precipitation Solution 100 mL $337
- +
LV825A-1 PEG-it Virus Precipitation Solution 250 mL $737
- +

Overview

Overview

Easily concentrate lentivirus particles for ultra-high titers

Used in over three-hundred citations, PEG-it™ Virus Concentration Reagent enables easy concentration of pseudoviral particles for achieving ultra-high titers. It’s great for concentrating pseudoviral particles even from large volumes of medium by removing the need for ultracentrifugation. Simply add PEG-it to the collected medium, incubate overnight at 4°C, and spin at 1500g for 30 minutes.

In addition, PEG-it acts as a cryopreservative for concentrated virus. Lentivirus concentrated with PEG-it lasts longer in the freezer and survives multiple freeze-thaw cycles with minimal loss of titer.

How It Works

How It Works

PEG-it is an easy way to get higher viral titers by concentrating virus

Using PEG-it to concentrate virus particles is easy with little hands-on time

Supporting Data

Supporting Data

PEG-it concentrates virus particles 10- to 100-fold

PEG-it concentrates virus particles 10- to 100-fold

Figure 1. PEG-it concentrates virus particles 10- to 100-fold. With PEG-it, virus particles can be concentrated up to 100-fold, leading to much higher infectious units per mL.

PEG-it is non-toxic and effective with stem cellsPEG-it is non-toxic and effective with stem cells

Figure 2. PEG-it is non-toxic and effective with Stem Cells. PEG-it-treated virus is non-toxic to stem cells and results in highly effective transductions. METHODS: H9 hES cells were transduced with pGreenZeo reporter constructs containing specific promoters for CMV, mOCT4 or mNANOG. Cells were cultured for eight weeks on Matrigel-coated plates with MEF conditioned medium containing 1 µg/mL Zeocin. The cells imaged here were split and grown on MEF feeder layers for four days. Data courtesy of Dr. Timothy Kamp and Chad H. Koonce, University of Wisconsin, Madison, Stem Cell and Regenerative Medicine Center.

PEG-it protects isolated virus particles from multiple freeze/thaw cyclesPEG-it protects isolated virus particles from multiple freeze/thaw cycles

Figure 3. PEG-it protects isolated virus particles from multiple freeze/thaw cycles. Lentivirus concentrated with PEG-it retains high titers even after four freeze-thaw cycles. HepG2 cells (top panel set) and HT1080 cells (bottom panel set) transduced with LV605VA-1 after 1 – 4 freeze-thaw cycles. Even after the fourth thaw, the LV605VA-1 virus particles show robust transduction efficiency.

Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective

Figure 4. Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective. The cells from Figure 3 were lysed and viral infectious units (IFUs) measured using the Global UltraRapid™ Lentiviral Titer Kit (Cat.# LV961A-1). No significant loss of IFUs due to repeated freeze/thaw cycles was found with virus particles that had been concentrated using PEG-it.

FAQs

PEG-it Virus Precipitation Solution is a reagent used to easily concentrate lentivirus particles for ultra-high titers, without the need for ultracentrifugation. It is suitable for concentrating pseudoviral particles from large volumes of medium and acts as a cryopreservative for concentrated virus.
PEG-it works by concentrating virus particles in a simple, hands-on procedure. Just add PEG-it to the collected medium, incubate overnight at 4°C, and spin at 1500g for 30 minutes. This process helps achieve higher viral titers by concentrating the virus.
PEG-it offers several benefits:
  • Concentrates virus particles 10- to 100-fold, leading to higher infectious units per mL
  • Eliminates the need for ultracentrifugation
  • Non-toxic and effective with stem cells
  • Acts as a cryopreservative, protecting the virus from multiple freeze-thaw cycles with minimal loss of titer
PEG-it acts as a cryopreservative for concentrated virus, allowing it to last longer in the freezer and survive multiple freeze-thaw cycles with minimal loss of titer.
Yes, PEG-it-treated virus is non-toxic to stem cells and results in highly effective transductions.
To speak to a specialist, you can contact System Biosciences at info@systembio.com or call our toll-free number at 1-(888) 640-4371.
PEG-it Virus Precipitation Solution is available in two sizes:
100 mL (Catalog Number: LV810A-1)
250 mL (Catalog Number: LV825A-1)
The user manual and product sheet for PEG-it Lentivirus Concentration Reagent can be found on the manufacturer's website under the "Resources" section.

Citations

  • An, M, et al. (2024) Engineered virus-like particles for transient delivery of prime editor ribonucleoprotein complexes in vivo. Nature biotechnology. 2024;. PM ID: 38191664
  • Ullah, I, et al. (2024) Bioluminescence Imaging Reveals Enhanced SARS-CoV-2 Clearance in Mice with Combinatorial Regimens. iScience. 2024;:109049. Link: iScience
  • Baudrier, L, et al. (2024) One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics. Cell reports methods. 2024;:100698. PM ID: 38301655
  • Konishi, CT, et al. (2024) Modeling and Correction of Protein Conformational Disease in iPSC-derived Neurons through Personalized Base Editing. bioRxiv : the preprint server for biology. 2024;. PM ID: 38293034
  • Botten, GA, Lee, M & Xu, J. (2023) Dissecting Locus-Specific Chromatin Interactions by CRISPR CAPTURE. Methods in molecular biology (Clifton, N.J.). 2023; 2599:69-97. PM ID: 36427144
  • Chen, Y, et al. (2023) Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD. iScience. 2023; 26(1):105783. PM ID: 36514310
  • Mlynarczyk, C, et al. (2023) BTG1 mutation yields supercompetitive B cells primed for malignant transformation. Science (New York, N.Y.). 2023; 379(6629):eabj7412. PM ID: 36656933
  • Li, X, et al. (2023) BMX controls 3βHSD1 and sex steroid biosynthesis in cancer. The Journal of clinical investigation. 2023; 133(2). PM ID: 36647826
  • Kang, H, et al. (2023) DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastoma. Cell reports. Medicine. 2023; 4(1):100880. PM ID: 36603576
  • Gurska, L, et al. (2023) Crizotinib Has Preclinical Efficacy in Philadelphia-Negative Myeloproliferative Neoplasms. Clinical Cancer Research. 2023;:OF1-OF14. Link: Clinical Cancer Research
  • Suzuki, T, et al. (2023) Pivotal Role of Ubiquitin Carboxyl-Terminal Hydrolase L1 (UCHL1) in Uterine Leiomyoma. Biomolecules. 2023; 13(2):193. Link: Biomolecules
  • Aw, WY, et al. (2023) Microphysiological model of PIK3CA-driven vascular malformations reveals a role of dysregulated Rac1 and mTORC1/2 in lesion formation. Science advances. 2023; 9(7):eade8939. PM ID: 36791204
  • Mitchell, K, et al. (2023) WDR5 represents a therapeutically exploitable target for cancer stem cells in glioblastoma. Genes & development. 2023;. PM ID: 36732025
  • Cai, Q, et al. (2023) Differential roles of CaMKII isoforms in phase separation with NMDA receptors and in synaptic plasticity. Cell reports. 2023; 42(3):112146. PM ID: 36827181
  • Li, S, et al. (2023) Dual targeting of protein translation and nuclear protein export results in enhanced anti-myeloma effects. Blood advances. 2023;. PM ID: 36827679
  • Wang, JH, et al. (2023) Development of a CRISPRi Human Retinal Pigmented Epithelium Model for Functional Study of Age-Related Macular Degeneration Genes. International journal of molecular sciences. 2023; 24(4). PM ID: 36834828
  • Ramos, E, et al. (2023) Novel Chaphamaparvovirus in Insectivorous Molossus molossus Bats, from the Brazilian Amazon Region. Viruses. 2023; 15(3):606. Link: Viruses
  • Decaesteker, B, et al. (2023) SOX11 regulates SWI/SNF complex components as member of the adrenergic neuroblastoma core regulatory circuitry. Nature communications. 2023; 14(1):1267. PM ID: 36882421
  • Cao, W, et al. (2023) TRIB2 safeguards naive T cell homeostasis during aging. Cell reports. 2023; 42(3):112195. PM ID: 36884349
  • McCann, JJ, et al. (2023) Participation of ATM, SMG1, and DDX5 in a DNA Damage-induced Alternative Splicing Pathway. Radiation research. 2023;. PM ID: 36921295