PEG-it Virus Precipitation Solution

Easily concentrate lentivirus with an ultracentrifugation-free protocol—great for scaled-up virus production and also protects virus from freeze-thaw cycles

Products

Catalog Number Description Size Price Quantity Add to Cart
LV810A-1 PEG-it Virus Precipitation Solution 100 mL $312.00
- +
Contact Us
LV825A-1 PEG-it Virus Precipitation Solution 250 mL $682.00
- +
Contact Us

Overview

Overview

Easily concentrate lentivirus particles for ultra-high titers

Used in over three-hundred citations, PEG-it™ Virus Concentration Reagent enables easy concentration of pseudoviral particles for achieving ultra-high titers. It’s great for concentrating pseudoviral particles even from large volumes of medium by removing the need for ultracentrifugation. Simply add PEG-it to the collected medium, incubate overnight at 4°C, and spin at 1500g for 30 minutes.

In addition, PEG-it acts as a cryopreservative for concentrated virus. Lentivirus concentrated with PEG-it lasts longer in the freezer and survives multiple freeze-thaw cycles with minimal loss of titer.

How It Works

How It Works

PEG-it is an easy way to get higher viral titers by concentrating virus

Using PEG-it to concentrate virus particles is easy with little hands-on time

Supporting Data

Supporting Data

PEG-it concentrates virus particles 10- to 100-fold

PEG-it concentrates virus particles 10- to 100-fold

Figure 1. PEG-it concentrates virus particles 10- to 100-fold. With PEG-it, virus particles can be concentrated up to 100-fold, leading to much higher infectious units per mL.

PEG-it is non-toxic and effective with stem cellsPEG-it is non-toxic and effective with stem cells

Figure 2. PEG-it is non-toxic and effective with Stem Cells. PEG-it-treated virus is non-toxic to stem cells and results in highly effective transductions. METHODS: H9 hES cells were transduced with pGreenZeo reporter constructs containing specific promoters for CMV, mOCT4 or mNANOG. Cells were cultured for eight weeks on Matrigel-coated plates with MEF conditioned medium containing 1 µg/mL Zeocin. The cells imaged here were split and grown on MEF feeder layers for four days. Data courtesy of Dr. Timothy Kamp and Chad H. Koonce, University of Wisconsin, Madison, Stem Cell and Regenerative Medicine Center.

PEG-it protects isolated virus particles from multiple freeze/thaw cyclesPEG-it protects isolated virus particles from multiple freeze/thaw cycles

Figure 3. PEG-it protects isolated virus particles from multiple freeze/thaw cycles. Lentivirus concentrated with PEG-it retains high titers even after four freeze-thaw cycles. HepG2 cells (top panel set) and HT1080 cells (bottom panel set) transduced with LV605VA-1 after 1 – 4 freeze-thaw cycles. Even after the fourth thaw, the LV605VA-1 virus particles show robust transduction efficiency.

Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective

Figure 4. Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective. The cells from Figure 3 were lysed and viral infectious units (IFUs) measured using the Global UltraRapid™ Lentiviral Titer Kit (Cat.# LV961A-1). No significant loss of IFUs due to repeated freeze/thaw cycles was found with virus particles that had been concentrated using PEG-it.

Resources

Citations

  • Kim, C, et al. (2021) Histone deficiency and accelerated replication stress in T cell aging. The Journal of clinical investigation. 1970 Jan 1; 131(11). PM ID: 34060486
  • Maru, Y, et al. (2021) Kras activation in endometrial organoids drives cellular transformation and epithelial-mesenchymal transition. Oncogenesis. 1970 Jan 1; 10(6):46. PM ID: 34172714
  • Lindner, B, et al. (2021) A genome-wide CRISPR/Cas9 screen to identify phagocytosis modulators in monocytic THP-1 cells. Scientific reports. 1970 Jan 1; 11(1):12973. PM ID: 34155263
  • Lo, YH, et al. (2021) A CRISPR/Cas9-engineered ARID1A-deficient human gastric cancer organoid model reveals essential and non-essential modes of oncogenic transformation. Cancer discovery. 1970 Jan 1;. PM ID: 33451982
  • Ni, M, et al. (2021) A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy. Genetics in medicine : official journal of the American College of Medical Genetics. 1970 Jan 1;. PM ID: 33473208
  • Kamo, M, et al. (2021) Discovery of anti-cell migration activity of an anti-HIV heterocyclic compound by identification of its binding protein hnRNP M. Bioorganic chemistry. 1970 Jan 1; 107:104627. PM ID: 33476868
  • Tinker, RJ, et al. (2021) Norovirus strains in patients with acute gastroenteritis in rural and low-income urban areas in northern Brazil. Archives of virology. 1970 Jan 1;. PM ID: 33462673
  • Kohli, J, et al. (2021) Combination of MCL-1 and BCL-2 Inhibitors Synergistically Kills Senescent Melanocytes and Leads to Removal of Melanocytic Nevi. SSRN Electronic Journal. 1970 Jan 1;. Link: SSRN Electronic Journal
  • Jiang, Z, et al. (2021) TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Yong, X, et al. (2021) Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIα phosphorylation of the GluN2A subunit. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Clayton, KL, et al. (2021) HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses. Cell host & microbe. 1970 Jan 1;. PM ID: 33571449
  • Yi, F, et al. (2021) The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis. Science advances. 1970 Jan 1; 7(9). PM ID: 33627431
  • Phasuk, S, et al. (2021) Enhanced contextual fear memory in peroxiredoxin 6 knockout mice is associated with hyperactivation of MAPK signaling pathway. Molecular brain. 1970 Jan 1; 14(1):42. PM ID: 33632301
  • de Oliveira Ribeiro, G, et al. (2021) Guapiaçu virus, a new insect-specific flavivirus isolated from two species of Aedes mosquitoes from Brazil. Scientific reports. 1970 Jan 1; 11(1):4674. PM ID: 33633167
  • Schiff, AE, et al. (2021) T cell-tropic HIV efficiently infects alveolar macrophages through contact with infected CD4+ T cells. Scientific reports. 1970 Jan 1; 11(1):3890. PM ID: 33594125
  • Lu, Q, et al. (2021) p75NTR/proBDNF Modulates Basal Cell Carcinoma (BCC) Immune Microenvironment via Necroptosis Signaling Pathway. Journal of immunology research. 1970 Jan 1; 2021:6652846. PM ID: 33604392
  • Ciciriello, A, et al. (2021) IL-10 lentivirus-laden hydrogel tubes increase spinal progenitor survival and neuronal differentiation after spinal cord injury. Authorea. 1970 Jan 1;. Link: Authorea
  • Cherbonneau, F, et al. (2021) TRACE-Seq: A Transgenic System for Unbiased Loading of mRNA into Extracellular Vesicles Allowing Non-Invasive Transcriptome Profiling of Living Cells. SSRN Electronic Journal. 1970 Jan 1;. Link: SSRN Electronic Journal
  • Ligeon, LA, et al. (2021) Oxidation inhibits autophagy protein deconjugation from phagosomes to sustain MHC class II restricted antigen presentation. Nature communications. 1970 Jan 1; 12(1):1508. PM ID: 33686057
  • Gui, L, et al. (2021) Role of Sox2 in Learning, Memory, and Postoperative Cognitive Dysfunction in Mice. Cells. 1970 Jan 1; 10(4):727. Link: Cells

Products

Catalog Number Description Size Price Quantity Add to Cart
LV810A-1 PEG-it Virus Precipitation Solution 100 mL $312.00
- +
Contact Us
LV825A-1 PEG-it Virus Precipitation Solution 250 mL $682.00
- +
Contact Us

Overview

Overview

Easily concentrate lentivirus particles for ultra-high titers

Used in over three-hundred citations, PEG-it™ Virus Concentration Reagent enables easy concentration of pseudoviral particles for achieving ultra-high titers. It’s great for concentrating pseudoviral particles even from large volumes of medium by removing the need for ultracentrifugation. Simply add PEG-it to the collected medium, incubate overnight at 4°C, and spin at 1500g for 30 minutes.

In addition, PEG-it acts as a cryopreservative for concentrated virus. Lentivirus concentrated with PEG-it lasts longer in the freezer and survives multiple freeze-thaw cycles with minimal loss of titer.

How It Works

How It Works

PEG-it is an easy way to get higher viral titers by concentrating virus

Using PEG-it to concentrate virus particles is easy with little hands-on time

Supporting Data

Supporting Data

PEG-it concentrates virus particles 10- to 100-fold

PEG-it concentrates virus particles 10- to 100-fold

Figure 1. PEG-it concentrates virus particles 10- to 100-fold. With PEG-it, virus particles can be concentrated up to 100-fold, leading to much higher infectious units per mL.

PEG-it is non-toxic and effective with stem cellsPEG-it is non-toxic and effective with stem cells

Figure 2. PEG-it is non-toxic and effective with Stem Cells. PEG-it-treated virus is non-toxic to stem cells and results in highly effective transductions. METHODS: H9 hES cells were transduced with pGreenZeo reporter constructs containing specific promoters for CMV, mOCT4 or mNANOG. Cells were cultured for eight weeks on Matrigel-coated plates with MEF conditioned medium containing 1 µg/mL Zeocin. The cells imaged here were split and grown on MEF feeder layers for four days. Data courtesy of Dr. Timothy Kamp and Chad H. Koonce, University of Wisconsin, Madison, Stem Cell and Regenerative Medicine Center.

PEG-it protects isolated virus particles from multiple freeze/thaw cyclesPEG-it protects isolated virus particles from multiple freeze/thaw cycles

Figure 3. PEG-it protects isolated virus particles from multiple freeze/thaw cycles. Lentivirus concentrated with PEG-it retains high titers even after four freeze-thaw cycles. HepG2 cells (top panel set) and HT1080 cells (bottom panel set) transduced with LV605VA-1 after 1 – 4 freeze-thaw cycles. Even after the fourth thaw, the LV605VA-1 virus particles show robust transduction efficiency.

Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective

Figure 4. Quantitative measurement of viral titer supports the conclusion from the imaging data that PEG-it is cryo-protective. The cells from Figure 3 were lysed and viral infectious units (IFUs) measured using the Global UltraRapid™ Lentiviral Titer Kit (Cat.# LV961A-1). No significant loss of IFUs due to repeated freeze/thaw cycles was found with virus particles that had been concentrated using PEG-it.

Citations

  • Kim, C, et al. (2021) Histone deficiency and accelerated replication stress in T cell aging. The Journal of clinical investigation. 1970 Jan 1; 131(11). PM ID: 34060486
  • Maru, Y, et al. (2021) Kras activation in endometrial organoids drives cellular transformation and epithelial-mesenchymal transition. Oncogenesis. 1970 Jan 1; 10(6):46. PM ID: 34172714
  • Lindner, B, et al. (2021) A genome-wide CRISPR/Cas9 screen to identify phagocytosis modulators in monocytic THP-1 cells. Scientific reports. 1970 Jan 1; 11(1):12973. PM ID: 34155263
  • Lo, YH, et al. (2021) A CRISPR/Cas9-engineered ARID1A-deficient human gastric cancer organoid model reveals essential and non-essential modes of oncogenic transformation. Cancer discovery. 1970 Jan 1;. PM ID: 33451982
  • Ni, M, et al. (2021) A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy. Genetics in medicine : official journal of the American College of Medical Genetics. 1970 Jan 1;. PM ID: 33473208
  • Kamo, M, et al. (2021) Discovery of anti-cell migration activity of an anti-HIV heterocyclic compound by identification of its binding protein hnRNP M. Bioorganic chemistry. 1970 Jan 1; 107:104627. PM ID: 33476868
  • Tinker, RJ, et al. (2021) Norovirus strains in patients with acute gastroenteritis in rural and low-income urban areas in northern Brazil. Archives of virology. 1970 Jan 1;. PM ID: 33462673
  • Kohli, J, et al. (2021) Combination of MCL-1 and BCL-2 Inhibitors Synergistically Kills Senescent Melanocytes and Leads to Removal of Melanocytic Nevi. SSRN Electronic Journal. 1970 Jan 1;. Link: SSRN Electronic Journal
  • Jiang, Z, et al. (2021) TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Yong, X, et al. (2021) Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIα phosphorylation of the GluN2A subunit. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Clayton, KL, et al. (2021) HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses. Cell host & microbe. 1970 Jan 1;. PM ID: 33571449
  • Yi, F, et al. (2021) The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis. Science advances. 1970 Jan 1; 7(9). PM ID: 33627431
  • Phasuk, S, et al. (2021) Enhanced contextual fear memory in peroxiredoxin 6 knockout mice is associated with hyperactivation of MAPK signaling pathway. Molecular brain. 1970 Jan 1; 14(1):42. PM ID: 33632301
  • de Oliveira Ribeiro, G, et al. (2021) Guapiaçu virus, a new insect-specific flavivirus isolated from two species of Aedes mosquitoes from Brazil. Scientific reports. 1970 Jan 1; 11(1):4674. PM ID: 33633167
  • Schiff, AE, et al. (2021) T cell-tropic HIV efficiently infects alveolar macrophages through contact with infected CD4+ T cells. Scientific reports. 1970 Jan 1; 11(1):3890. PM ID: 33594125
  • Lu, Q, et al. (2021) p75NTR/proBDNF Modulates Basal Cell Carcinoma (BCC) Immune Microenvironment via Necroptosis Signaling Pathway. Journal of immunology research. 1970 Jan 1; 2021:6652846. PM ID: 33604392
  • Ciciriello, A, et al. (2021) IL-10 lentivirus-laden hydrogel tubes increase spinal progenitor survival and neuronal differentiation after spinal cord injury. Authorea. 1970 Jan 1;. Link: Authorea
  • Cherbonneau, F, et al. (2021) TRACE-Seq: A Transgenic System for Unbiased Loading of mRNA into Extracellular Vesicles Allowing Non-Invasive Transcriptome Profiling of Living Cells. SSRN Electronic Journal. 1970 Jan 1;. Link: SSRN Electronic Journal
  • Ligeon, LA, et al. (2021) Oxidation inhibits autophagy protein deconjugation from phagosomes to sustain MHC class II restricted antigen presentation. Nature communications. 1970 Jan 1; 12(1):1508. PM ID: 33686057
  • Gui, L, et al. (2021) Role of Sox2 in Learning, Memory, and Postoperative Cognitive Dysfunction in Mice. Cells. 1970 Jan 1; 10(4):727. Link: Cells