pCDH-EF1α-MCS-BGH-PGK-GFP-T2A-Puro Cloning and Expression Lentivector
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CD550A-1 | pCDH-EF1α-MCS-BGH-PGK-GFP-T2A-Puro cDNA Dual Promoter Cloning and Expression Lentivector | 10 µg | $609 |
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Overview
Overview
Get robust, stable expression of your gene-of-interest in dividing and quiescent cells
Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-BGH-PGK-GFP-T2A-Puro Cloning and Expression Lentivector drives expression of your gene-of-interest from an EF1α promoter, and co-expression of GFP and puromycin resistance from a PGK promoter. The addition of a BGH poly-A site in between the MCS into which you clone your gene-of-interest and the downstream PGK promoter enhances EF1α-driven expression of your cDNA clone.
With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
| Promoter | Expression Level | Application |
|---|---|---|
| CMV | High | Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.) |
| MSCV | High | Hematopoietic and stem cells |
| EF1α | Medium | Most cell types including primary cells and stem cells |
| PGK | Medium | Most cell types including primary cells and stem cells |
| UbC | Low | Most cell types including primary cells and stem cells |
References
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Supporting Data
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Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CD550A-1 | pCDH-EF1α-MCS-BGH-PGK-GFP-T2A-Puro cDNA Dual Promoter Cloning and Expression Lentivector | 10 µg | $609 |
|
||||
Overview
Overview
Get robust, stable expression of your gene-of-interest in dividing and quiescent cells
Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-BGH-PGK-GFP-T2A-Puro Cloning and Expression Lentivector drives expression of your gene-of-interest from an EF1α promoter, and co-expression of GFP and puromycin resistance from a PGK promoter. The addition of a BGH poly-A site in between the MCS into which you clone your gene-of-interest and the downstream PGK promoter enhances EF1α-driven expression of your cDNA clone.
With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
| Promoter | Expression Level | Application |
|---|---|---|
| CMV | High | Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.) |
| MSCV | High | Hematopoietic and stem cells |
| EF1α | Medium | Most cell types including primary cells and stem cells |
| PGK | Medium | Most cell types including primary cells and stem cells |
| UbC | Low | Most cell types including primary cells and stem cells |