pCDH-CMV-MCS-EF1α-RFP Cloning and Expression Lentivector

With the strong CMV promoter upstream of an MCS and EF1α driving the RFP reporter, pCDH-CMV-MCS-EF1α-RFP is a great, all-purpose lentivector
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter

Products

Catalog Number Description Size Price Quantity Add to Cart
CD512B-1 pCDH-CMV-MCS-EF1α-RFP cDNA Dual Promoter Cloning and Expression Lentivector 10 µg $593
- +
CD512VA-1 pCDH-CMV-MCS-EF1α-RFP cDNA Dual Promoter Cloning and Expression Lentivector (pre-packaged virus) >1 x 10^6 IFUs $747
- +

Overview

Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-CMV-MCS-EF1α-RFP Cloning and Expression Lentivector drives expression of your gene-of-interest from the strong CMV promoter, and expression of the RFP reporter from the EF1α promoter.

pCDH-CMV-MCS-EF1α-RFP Cloning and Expression Lentivector With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
PromoterExpression LevelApplication
CMVHighCommonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCVHighHematopoietic and stem cells
EF1αMediumMost cell types including primary cells and stem cells
PGKMediumMost cell types including primary cells and stem cells
UbCLowMost cell types including primary cells and stem cells

How It Works

Supporting Data

FAQs

Resources

Citations

  • Sarangi, SK, Lande, KM & Kumar, S. (2023) KIR signaling is regulated by electrostatic interaction of its cytosolic tail with the plasma membrane despite being neutral polyampholyte. Proceedings of the National Academy of Sciences of the United States of America. 2023; 120(1):e2212987120. PM ID: 36574700
  • Fang, Z, et al. (2023) Oxidative stress-triggered Wnt signaling perturbation characterizes the tipping point of lung adeno-to-squamous transdifferentiation. Signal transduction and targeted therapy. 2023; 8(1):16. PM ID: 36627278
  • Lee, SA, et al. (2023) OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis. Nature cell biology. 2023; 25(1):92-107. PM ID: 36604592
  • Li, C, et al. (2023) Novel CD19-specific γ/δ TCR-T cells in relapsed or refractory diffuse large B-cell lymphoma. Journal of hematology & oncology. 2023; 16(1):5. PM ID: 36681817
  • Zhao, S, et al. (2023) Salmonella effector SopB reorganizes cytoskeletal vimentin to maintain replication vacuoles for efficient infection. Nature communications. 2023; 14(1):478. PM ID: 36717589
  • Crowley, MJP, et al. (2023) Tumor-intrinsic IRE1α signaling controls protective immunity in lung cancer. Nature communications. 2023; 14(1):120. PM ID: 36624093
  • Wang, SQ, et al. (2023) Targeting GSTP1 as Therapeutic Strategy against Lung Adenocarcinoma Stemness and Resistance to Tyrosine Kinase Inhibitors. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2023;:e2205262. PM ID: 36709476
  • Wang, R, et al. (2023) ZBTB18 restricts chromatin accessibility and prevents transcriptional adaptations that drive metastasis. Science advances. 2023; 9(1):eabq3951. PM ID: 36608120
  • Yang, C, et al. (2023) Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules. Autophagy. 2023;:1-18. PM ID: 36692217
  • Sahu, S, et al. (2023) Nucleolar Architecture Is Modulated by a Small Molecule, the Inositol Pyrophosphate 5-InsP7. Biomolecules. 2023; 13(1). PM ID: 36671538
  • Xi, S, et al. (2023) Cigarette Smoke Enhances the Malignant Phenotype of Esophageal Adenocarcinoma Cells by Disrupting a Repressive Regulatory Interaction Between miR-145 and LOXL2. Laboratory Investigation. 2023; 103(4):100014. Link: Laboratory Investigation
  • Tsubosaka, M, et al. (2023) The efficiency of genetically modified mesenchymal stromal cells combined with a functionally graded scaffold for bone regeneration in corticosteroid-induced osteonecrosis of the femoral head in rabbits. Journal of biomedical materials research. Part A. 2023;. PM ID: 36606330
  • Guo, C, et al. (2023) Engagement of an optimized lentiviral vector enhances the expression and cytotoxicity of CAR in human NK cells. Molecular Immunology. 2023; 155:91-99. Link: Molecular Immunology
  • Zhang, Y, et al. (2023) Development of stable HEK293T cell pools expressing CSFV E2 protein: A potential antigen expression platform. Vaccine. 2023;. PM ID: 36725430
  • Leland, P, et al. (2023) Characterization of Chimeric Antigen Receptor Modified T Cells Expressing scFv-IL-13Rα2 after Radiolabeling with 89Zirconium Oxine for PET Imaging. Research square. 2023;. PM ID: 36711796
  • Yi, L, et al. (2023) Aurora kinase inhibitors regulate T memory stem cell phenotype in T cell receptor-engineered T cells with prolonged persistence. Research Square. 2023;. Link: Research Square
  • ROBINSON, K, et al. (2023) Corynebacterium diphtheriaecauses keratinocyte-intrinsic ribotoxic stress and NLRP1 inflammasome activation in a model of cutaneous diphtheria. bioRxiv. 2023;. Link: bioRxiv
  • An, H, et al. (2023) Identification of the mouse Kupffer cell receptors recognizing pneumococcal capsules by affinity screening. STAR Protocols. 2023; 4(1):102065. Link: STAR Protocols
  • 韩, 留. (2023) Construction of Non-Small Cell Lung Cancer (NSCLC) Cell Lines Stably Overexpressing MST4 or dnMST4 Transgenes. Advances in Clinical Medicine. 2023; 13(01):191-200. Link: Advances in Clinical Medicine
  • Stenzel, M. (2023) 12/15-lipoxygenase and its role in anti-tumor immunity. Thesis. 2023;. Link: Thesis

Products

Catalog Number Description Size Price Quantity Add to Cart
CD512B-1 pCDH-CMV-MCS-EF1α-RFP cDNA Dual Promoter Cloning and Expression Lentivector 10 µg $593
- +
CD512VA-1 pCDH-CMV-MCS-EF1α-RFP cDNA Dual Promoter Cloning and Expression Lentivector (pre-packaged virus) >1 x 10^6 IFUs $747
- +

Overview

Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-CMV-MCS-EF1α-RFP Cloning and Expression Lentivector drives expression of your gene-of-interest from the strong CMV promoter, and expression of the RFP reporter from the EF1α promoter.

pCDH-CMV-MCS-EF1α-RFP Cloning and Expression Lentivector With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
PromoterExpression LevelApplication
CMVHighCommonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCVHighHematopoietic and stem cells
EF1αMediumMost cell types including primary cells and stem cells
PGKMediumMost cell types including primary cells and stem cells
UbCLowMost cell types including primary cells and stem cells

How It Works

Supporting Data

FAQs

Citations

  • Sarangi, SK, Lande, KM & Kumar, S. (2023) KIR signaling is regulated by electrostatic interaction of its cytosolic tail with the plasma membrane despite being neutral polyampholyte. Proceedings of the National Academy of Sciences of the United States of America. 2023; 120(1):e2212987120. PM ID: 36574700
  • Fang, Z, et al. (2023) Oxidative stress-triggered Wnt signaling perturbation characterizes the tipping point of lung adeno-to-squamous transdifferentiation. Signal transduction and targeted therapy. 2023; 8(1):16. PM ID: 36627278
  • Lee, SA, et al. (2023) OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis. Nature cell biology. 2023; 25(1):92-107. PM ID: 36604592
  • Li, C, et al. (2023) Novel CD19-specific γ/δ TCR-T cells in relapsed or refractory diffuse large B-cell lymphoma. Journal of hematology & oncology. 2023; 16(1):5. PM ID: 36681817
  • Zhao, S, et al. (2023) Salmonella effector SopB reorganizes cytoskeletal vimentin to maintain replication vacuoles for efficient infection. Nature communications. 2023; 14(1):478. PM ID: 36717589
  • Crowley, MJP, et al. (2023) Tumor-intrinsic IRE1α signaling controls protective immunity in lung cancer. Nature communications. 2023; 14(1):120. PM ID: 36624093
  • Wang, SQ, et al. (2023) Targeting GSTP1 as Therapeutic Strategy against Lung Adenocarcinoma Stemness and Resistance to Tyrosine Kinase Inhibitors. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2023;:e2205262. PM ID: 36709476
  • Wang, R, et al. (2023) ZBTB18 restricts chromatin accessibility and prevents transcriptional adaptations that drive metastasis. Science advances. 2023; 9(1):eabq3951. PM ID: 36608120
  • Yang, C, et al. (2023) Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules. Autophagy. 2023;:1-18. PM ID: 36692217
  • Sahu, S, et al. (2023) Nucleolar Architecture Is Modulated by a Small Molecule, the Inositol Pyrophosphate 5-InsP7. Biomolecules. 2023; 13(1). PM ID: 36671538
  • Xi, S, et al. (2023) Cigarette Smoke Enhances the Malignant Phenotype of Esophageal Adenocarcinoma Cells by Disrupting a Repressive Regulatory Interaction Between miR-145 and LOXL2. Laboratory Investigation. 2023; 103(4):100014. Link: Laboratory Investigation
  • Tsubosaka, M, et al. (2023) The efficiency of genetically modified mesenchymal stromal cells combined with a functionally graded scaffold for bone regeneration in corticosteroid-induced osteonecrosis of the femoral head in rabbits. Journal of biomedical materials research. Part A. 2023;. PM ID: 36606330
  • Guo, C, et al. (2023) Engagement of an optimized lentiviral vector enhances the expression and cytotoxicity of CAR in human NK cells. Molecular Immunology. 2023; 155:91-99. Link: Molecular Immunology
  • Zhang, Y, et al. (2023) Development of stable HEK293T cell pools expressing CSFV E2 protein: A potential antigen expression platform. Vaccine. 2023;. PM ID: 36725430
  • Leland, P, et al. (2023) Characterization of Chimeric Antigen Receptor Modified T Cells Expressing scFv-IL-13Rα2 after Radiolabeling with 89Zirconium Oxine for PET Imaging. Research square. 2023;. PM ID: 36711796
  • Yi, L, et al. (2023) Aurora kinase inhibitors regulate T memory stem cell phenotype in T cell receptor-engineered T cells with prolonged persistence. Research Square. 2023;. Link: Research Square
  • ROBINSON, K, et al. (2023) Corynebacterium diphtheriaecauses keratinocyte-intrinsic ribotoxic stress and NLRP1 inflammasome activation in a model of cutaneous diphtheria. bioRxiv. 2023;. Link: bioRxiv
  • An, H, et al. (2023) Identification of the mouse Kupffer cell receptors recognizing pneumococcal capsules by affinity screening. STAR Protocols. 2023; 4(1):102065. Link: STAR Protocols
  • 韩, 留. (2023) Construction of Non-Small Cell Lung Cancer (NSCLC) Cell Lines Stably Overexpressing MST4 or dnMST4 Transgenes. Advances in Clinical Medicine. 2023; 13(01):191-200. Link: Advances in Clinical Medicine
  • Stenzel, M. (2023) 12/15-lipoxygenase and its role in anti-tumor immunity. Thesis. 2023;. Link: Thesis