pCDH-CMV-MCS-EF1α-copGFP Cloning and Expression Lentivector

With the strong CMV promoter upstream of an MCS and EF1α driving the copepod GFP reporter, pCDH-CMV-MCS-EF1α-copGFP is a great, all-purpose lentivector
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter

Products

Catalog Number Description Size Price Quantity Add to Cart
CD511B-1 pCDH-CMV-MCS-EF1α-copGFP cDNA Dual Promoter Cloning and Expression Lentivector 10 µg $561
- +
CD511VB-1 pCDH-CMV-MCS-EF1α-copGFP cDNA Dual Promoter Cloning and Expression Lentivector (pre-packaged virus) >1 x 10^6 IFUs $706
- +
CD511VB-UHT pCDH-CMV-MCS-EF1α-copGFP cDNA Dual Promoter Cloning and Expression Lentivector (pre-packaged Ultra-High Titer virus, >1 x 10^9 IFU/mL) >10 x 10^6 IFUs $950
- +

Overview

Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-CMV-MCS-EF1α-copGFP Cloning and Expression Lentivector drives expression of your gene-of-interest from the strong CMV promoter, and expression of the copepod GFP reporter from the EF1α promoter.

pCDH-CMV-MCS-EF1α-copGFP Cloning and Expression Lentivector With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
PromoterExpression LevelApplication
CMVHighCommonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCVHighHematopoietic and stem cells
EF1αMediumMost cell types including primary cells and stem cells
PGKMediumMost cell types including primary cells and stem cells
UbCLowMost cell types including primary cells and stem cells

How It Works

Supporting Data

Resources

Citations

  • Liu, YY, et al. (2022) O-GlcNAcylation of MORC2 at threonine 556 by OGT couples TGF-β signaling to breast cancer progression. Cell death and differentiation. 1970 Jan 1;. PM ID: 34974534
  • Lockhart-Cairns, M, et al. (2022) Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation. Matrix Biology. 1970 Jan 1;. Link: Matrix Biology
  • Ma, S, et al. (2022) PDGF-D-PDGFRβ signaling enhances IL-15-mediated human natural killer cell survival. Proceedings of the National Academy of Sciences of the United States of America. 1970 Jan 1; 119(3). PM ID: 35027451
  • Kuravi, S, et al. (2022) Functional characterization of NPM1-TYK2 fusion oncogene. NPJ precision oncology. 1970 Jan 1; 6(1):3. PM ID: 35042970
  • Kervarrec, T, et al. (2022) Recurrent FOXK1::GRHL and GPS2::GRHL fusions in trichogerminoma. The Journal of pathology. 1970 Jan 1;. PM ID: 35049062
  • Zhang, X, et al. (2022) T-Cell Immunoglobulin and Mucin Domain 1 (TIM-1) Is a Functional Entry Factor for Tick-Borne Encephalitis Virus. mBio. 1970 Jan 1;:e0286021. PM ID: 35073759
  • Richter, A, et al. (2022) The Molecular Subtype of Adult Acute Lymphoblastic Leukemia Samples Determines the Engraftment Site and Proliferation Kinetics in Patient-Derived Xenograft Models. Cells. 1970 Jan 1; 11(1). PM ID: 35011712
  • Kim, WK, et al. (2022) Heat-Shock Proteins Can Potentiate the Therapeutic Ability of Cryopreserved Mesenchymal Stem Cells for the Treatment of Acute Spinal Cord Injury in Dogs. Stem cell reviews and reports. 1970 Jan 1;. PM ID: 35001344
  • Gao, Y, et al. (2022) Golgi phosphoprotein 3 promotes angiogenesis and sorafenib resistance in hepatocellular carcinoma via upregulating exosomal miR-494-3p. Cancer cell international. 1970 Jan 1; 22(1):35. PM ID: 35073936
  • Khan, AA, et al. (2022) WBSCR22 and TRMT112 synergistically suppress cell proliferation, invasion and tumorigenesis in pancreatic cancer via transcriptional regulation of ISG15. International journal of oncology. 1970 Jan 1; 60(3). PM ID: 35088887
  • Ilina, E, et al. (2022) Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migration. iScience. 1970 Jan 1;:103842. Link: iScience
  • Kim, M, et al. (2022) A Recombinant Ig Fragment (IgCw-γεκ) Comprising the Cγ1-Cε2-4 and Cκ Domains Is an Alternative Reagent to Human IgE. Journal of immunology (Baltimore, Md. : 1950). 1970 Jan 1; 208(3):772-779. PM ID: 35022271
  • Martin, M, et al. (2022) The immune-specific E3 ubiquitin ligase MARCH1 is upregulated during HCMV infection to regulate iron levels. Journal of virology. 1970 Jan 1;:jvi0180621. PM ID: 35045264
  • Yang, B, et al. (2022) Human Cytomegalovirus Hijacks WD Repeat Domain 11 for Virion Assembly Compartment Formation and Virion Morphogenesis. Journal of virology. 1970 Jan 1;:JVI0182721. PM ID: 35020472
  • Huang, H, et al. (2022) TSPAN1-elevated FAM110A promotes pancreatic cancer progression by transcriptionally regulating HIST1H2BK. Journal of Cancer. 1970 Jan 1; 13(3):906-917. Link: Journal of Cancer
  • Vannini, I, et al. (2022) Overexpression of ultraconserved region 83- induces lung cancer tumorigenesis. PloS one. 1970 Jan 1; 17(1):e0261464. PM ID: 35015757
  • Lal, S & Raffel, C. (2022) Protocols to Manufacture an Oncolytic Measles Virus-Sensitive Immunocompetent Mouse Model of Medulloblastoma. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2423:165-177. PM ID: 34978698
  • Kono, Y, et al. (2022) Nucleoplasmic Lamin C Rapidly Accumulates at Sites of Nuclear Envelope Rupture with BAF and cGAS. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • 林浩, , et al. (2022) 分泌 anti-PD-L1 及 IFNα 的间充质干细胞联合治疗小鼠黑色素瘤. 中国免疫学杂志. 1970 Jan 1;. Link: 中国免疫学杂志
  • Pan, R, et al. (2022) Augmenting NK cell-based immunotherapy by targeting mitochondrial apoptosis. Cell. 1970 Jan 1; 185(9):1521-1538.e18. PM ID: 35447071
pCDH-CMV-MCS-EF1α-copGFP Cloning and Expression Lentivector $561.00

Products

Catalog Number Description Size Price Quantity Add to Cart
CD511B-1 pCDH-CMV-MCS-EF1α-copGFP cDNA Dual Promoter Cloning and Expression Lentivector 10 µg $561
- +
CD511VB-1 pCDH-CMV-MCS-EF1α-copGFP cDNA Dual Promoter Cloning and Expression Lentivector (pre-packaged virus) >1 x 10^6 IFUs $706
- +
CD511VB-UHT pCDH-CMV-MCS-EF1α-copGFP cDNA Dual Promoter Cloning and Expression Lentivector (pre-packaged Ultra-High Titer virus, >1 x 10^9 IFU/mL) >10 x 10^6 IFUs $950
- +

Overview

Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-CMV-MCS-EF1α-copGFP Cloning and Expression Lentivector drives expression of your gene-of-interest from the strong CMV promoter, and expression of the copepod GFP reporter from the EF1α promoter.

pCDH-CMV-MCS-EF1α-copGFP Cloning and Expression Lentivector With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
PromoterExpression LevelApplication
CMVHighCommonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCVHighHematopoietic and stem cells
EF1αMediumMost cell types including primary cells and stem cells
PGKMediumMost cell types including primary cells and stem cells
UbCLowMost cell types including primary cells and stem cells

How It Works

Supporting Data

Citations

  • Liu, YY, et al. (2022) O-GlcNAcylation of MORC2 at threonine 556 by OGT couples TGF-β signaling to breast cancer progression. Cell death and differentiation. 1970 Jan 1;. PM ID: 34974534
  • Lockhart-Cairns, M, et al. (2022) Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation. Matrix Biology. 1970 Jan 1;. Link: Matrix Biology
  • Ma, S, et al. (2022) PDGF-D-PDGFRβ signaling enhances IL-15-mediated human natural killer cell survival. Proceedings of the National Academy of Sciences of the United States of America. 1970 Jan 1; 119(3). PM ID: 35027451
  • Kuravi, S, et al. (2022) Functional characterization of NPM1-TYK2 fusion oncogene. NPJ precision oncology. 1970 Jan 1; 6(1):3. PM ID: 35042970
  • Kervarrec, T, et al. (2022) Recurrent FOXK1::GRHL and GPS2::GRHL fusions in trichogerminoma. The Journal of pathology. 1970 Jan 1;. PM ID: 35049062
  • Zhang, X, et al. (2022) T-Cell Immunoglobulin and Mucin Domain 1 (TIM-1) Is a Functional Entry Factor for Tick-Borne Encephalitis Virus. mBio. 1970 Jan 1;:e0286021. PM ID: 35073759
  • Richter, A, et al. (2022) The Molecular Subtype of Adult Acute Lymphoblastic Leukemia Samples Determines the Engraftment Site and Proliferation Kinetics in Patient-Derived Xenograft Models. Cells. 1970 Jan 1; 11(1). PM ID: 35011712
  • Kim, WK, et al. (2022) Heat-Shock Proteins Can Potentiate the Therapeutic Ability of Cryopreserved Mesenchymal Stem Cells for the Treatment of Acute Spinal Cord Injury in Dogs. Stem cell reviews and reports. 1970 Jan 1;. PM ID: 35001344
  • Gao, Y, et al. (2022) Golgi phosphoprotein 3 promotes angiogenesis and sorafenib resistance in hepatocellular carcinoma via upregulating exosomal miR-494-3p. Cancer cell international. 1970 Jan 1; 22(1):35. PM ID: 35073936
  • Khan, AA, et al. (2022) WBSCR22 and TRMT112 synergistically suppress cell proliferation, invasion and tumorigenesis in pancreatic cancer via transcriptional regulation of ISG15. International journal of oncology. 1970 Jan 1; 60(3). PM ID: 35088887
  • Ilina, E, et al. (2022) Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migration. iScience. 1970 Jan 1;:103842. Link: iScience
  • Kim, M, et al. (2022) A Recombinant Ig Fragment (IgCw-γεκ) Comprising the Cγ1-Cε2-4 and Cκ Domains Is an Alternative Reagent to Human IgE. Journal of immunology (Baltimore, Md. : 1950). 1970 Jan 1; 208(3):772-779. PM ID: 35022271
  • Martin, M, et al. (2022) The immune-specific E3 ubiquitin ligase MARCH1 is upregulated during HCMV infection to regulate iron levels. Journal of virology. 1970 Jan 1;:jvi0180621. PM ID: 35045264
  • Yang, B, et al. (2022) Human Cytomegalovirus Hijacks WD Repeat Domain 11 for Virion Assembly Compartment Formation and Virion Morphogenesis. Journal of virology. 1970 Jan 1;:JVI0182721. PM ID: 35020472
  • Huang, H, et al. (2022) TSPAN1-elevated FAM110A promotes pancreatic cancer progression by transcriptionally regulating HIST1H2BK. Journal of Cancer. 1970 Jan 1; 13(3):906-917. Link: Journal of Cancer
  • Vannini, I, et al. (2022) Overexpression of ultraconserved region 83- induces lung cancer tumorigenesis. PloS one. 1970 Jan 1; 17(1):e0261464. PM ID: 35015757
  • Lal, S & Raffel, C. (2022) Protocols to Manufacture an Oncolytic Measles Virus-Sensitive Immunocompetent Mouse Model of Medulloblastoma. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2423:165-177. PM ID: 34978698
  • Kono, Y, et al. (2022) Nucleoplasmic Lamin C Rapidly Accumulates at Sites of Nuclear Envelope Rupture with BAF and cGAS. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • 林浩, , et al. (2022) 分泌 anti-PD-L1 及 IFNα 的间充质干细胞联合治疗小鼠黑色素瘤. 中国免疫学杂志. 1970 Jan 1;. Link: 中国免疫学杂志
  • Pan, R, et al. (2022) Augmenting NK cell-based immunotherapy by targeting mitochondrial apoptosis. Cell. 1970 Jan 1; 185(9):1521-1538.e18. PM ID: 35447071