Mouse IBA-1 pGreenZeo Differentiation Reporter

Study neurogenesis by expressing GFP in cells where the IBA-1 promoter/enhancer is active
  • Create stable neurogenesis-reporting cell lines
  • Monitor multiple lineages simultaneously
  • Track differentiation in live cells in real time

Products

Catalog Number Description Size Price Quantity Add to Cart
SR10019PA-1 Mouse IBA-1 Differentiation Reporter (pGreenZeo, Plasmid) 10 µg $1,594.00
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Contact Us
SR10019VA-1 Mouse IBA-1 Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs $662.00
- +
Contact Us

Overview

Overview

Monitor neurogenesis in real time With SBI’s line of pGreenZeo, pRedZeo, and pRedTK Differentation Reporter Vectors, you can monitor stem cell differentiation in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built differentiation reporters come as ready-to-package lentivector plasmids or ready-to-transduce pre-packaged lentivirus. The Mouse IBA-1 pGreenZeo Differentiation Reporter co-expresses dscGFP (destabilized copGFP, 2-hour half-life) and zeomycin resistance from the mouse IBA-1 promoter/enhancer elements, enabling visualization of neurogenesis using GFP fluorescence and selection for the desired cells using zeomycin.
  • Create stable neurogenesis-reporting cell lines
  • Monitor multiple lineages simultaneously
  • Track differentiation in live cells in real time
Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes. Choose the differentiation reporter that’s right for you Neural differentiation reporters
Catalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional names
SR1008PA/VA-1Macrophage, microgliaMouseCd68Cd68macrosialin, Scard1, gp110
SR10015PA/VA-1AstrocyteHumanGFAPGFAPGlial fibrillary acidic protein
SR10016PA/VA-1AstrocyteMouseGfapGfapGlial fibrillary acidic protein
SR10017PA/VA-1MicrogliaHumanCD11bITGAMintegrin, alpha M, CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A
SR10018PA/VA-1MicrogliaMouseEMR1Emr1EGF-like module containing, mucin-like, hormone receptor-like sequence 1, EGF-TM7, F4/80
SR10019PA/VA-1MicrogliaMouseIba-1Aif1allograft inflammatory factor 1 (Aif-1), ionized calcium binding adapter molecule 1 (Iba-1)
SR10020PA/VA-1Muller gliaMouseCd44Cd44HERMES, Ly-24, Pgp-1
SR10021PA/VA-1NeuronHumanBM88Cend1cell cycle exit and neuronal differentiation 1 (Cend1)
SR10022PA/VA-1NeuronMouseCamk2aCamk2aCaMK II; alpha-CaMKII
SR10023PA/VA-1NeuronMouseGAD67Gad1glutamic acid decarboxylase 1, EP10, GAD67, Gad-1
SR10024PA/VA-1NeuronRatNSEEno2enolase 2, gamma (Eno2), neuron-specific enolase (NSE)
SR10025PA/VA-1NeuronMouseTa1 a-tubulinTuba1atubulin, alpha 1A (Tuba1a), Tuba-1, Tuba1
SR10026PA/VA-1OligodendrocyteMouseMBPMbpmyelin basic protein (Mbp), myelin deficient; shiverer
SR10027PA/VA-1PhotoreceptorHumanOpsinOPN1SWopsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan)
SR10034PA/VA-1Neural Stem CellRatNestinNesNes
SR10035PA/VA-1Neural Stem CellHumanNestinNESNES
SR10041PA/VA-1NeuronHumanDoublecortinDCXDBCN, DC, LISX, SCLH, XLIS
SR10047PA/VA-1NeuronHumanMAP2MAP2MAP2A, MAP2B, MAP2C
SR10048PA/VA-1NeuronHumanFABP7FABP7B-FABP, BLBP, DKFZp547J2313, FABPB, MRG
Myogenic differentiation reporters
Catalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional names
SR10010PA/VA-1CardiomyocyteMouseActcActc1Cardiac muscle alpha actin 1
SR10011PA/VA-1CardiomyocyteHumanMLC-2vMYL2MYL2, MLC2
SR10012PA/VA-1CardiomyocyteHumanTNNT2TNNT2Troponin T type 2 (cardiac), TnTC, cTnT
SR10013PA/VA-1CardiomyocyteMouseTnnt2Tnnt2Tnt, cTnT
SR10014PA/VA-1Smooth muscle myocyteMouseSM22aTaglnTransgelin (Tagln), SM22, Sm22a
SR10049PA/VA-1CardiomyocyteHumanACTCACTC1ACTC, CMD1R
SR10050PA/VA-1Skeletal myocyteMouseMyogeninMyogmyo; MYF4
Hematopoietic differentiation reporters
Catalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional names
SR1004PA/VA-1B-cellHumanB29CD79BCD79b, Ig-beta, Igb, Igbeta
SR1005PA/VA-1B-cellMouseB29Cd79bCd79b
SR1006PA/VA-1CD8 T-cellMouseCD8Cd8aBB154331, Ly-2, Ly-35, Ly-B, Lyt-2
SR1007PA/VA-1ErythroidHumanHLA-DRaHLA-DRAHLA-DRA1, major histocompatibility complex, class II, DR alpha
SR1008PA/VA-1Macrophage, microgliaMouseCd68Cd68macrosialin, Scard1, gp110
SR1009PA/VA-1Pan T-cellHumanCD2CD2SRBC, T11
SR10032PA/VA-1LymphocyteHumanLCKLCKYT16, p56lck, pp58lck
Structural differentiation reporters
Catalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional names
SR1001PA/VA-1ChondrocyteMouseCol2a1Col2a1collagen, type II, alpha 1
SR1002PA/VA-1OsteoblastHumanSPP1SPP1secreted phosphoprotein 1 (SPP1), osteopontin (OPN)
SR1003PA/VA-1OsteoblastHumanOsteocalcinBGLAPbone gamma-carboxyglutamate (BGLAP), BGP, PMF1
SR10036PA/VA-1AdipocyteMouseALBPFabp4422/aP2, ALBP/Ap2, Ap2, Lbpl
SR10038PA/VA-1EpitheliumHumanKeratin 14KRT14CK14, EBS3, EBS4, K14, NFJ
Signaling differentiation reporters
Catalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional names
SR10028PA/VA-1Beta cellHumanInsulinINSinsulin
SR10037PA/VA-1IsletHumanNGN3NGN3neurogenin 3, Atoh5, Math4B, bHLHa7, neurog3
SR10039PA/VA-1IsletHumanPDX1PDX1IDX-1, IPF1, IUF1, MODY4, PDX-1, STF-1
SR10040PA/VA-1IsletMousePdx1Pdx1IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1

How It Works

Supporting Data

Supporting Data

See some of our differentiation reporters in action

SBI’s differentiation reporters are used in a number of papers. The data shown below are just one example (from Ravin R, Hoeppner DJ, Munno DM, Carmel L, Sullivan J, Levitt DL, Miller JL, Athaide C, Panchision DM, McKay RD. Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 2008 Dec 4; 3(6):670-80. PMID: 19041783)

Live imaging of neuronal differentiation

Figure 1. Live imaging of neuronal differentiation. Ravin, et al, used SBI’s Mouse IBA-1 pGreenFire Differentiation Reporter (Cat.# SR10016VA-1), which drives GFP expression from the glial fibrillary acidic protein promoter, to watch mouse neural stem cells differentiate into a network of mature neurons, oligodendrocytes, and astrocytes over the course of seven days. The periodic “flashes” seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The final photo taken after the network formation is shown below the video (color added). Among the network of neurons, only the astrocytes are bright green, demonstrating the specificity of SBI’s mouse IBA-1 pGreenFire Differentiation Reporter.

Simultaneously track multiple lineages from iPS and progenitor cells

Figure 2. Simultaneously track multiple lineages from iPS and progenitor cells.

See SBI’s differentiation reporters in action

Figure 3. Additional differentiation reporter data.

Resources

Citations

Products

Catalog Number Description Size Price Quantity Add to Cart
SR10019PA-1 Mouse IBA-1 Differentiation Reporter (pGreenZeo, Plasmid) 10 µg $1,594.00
- +
Contact Us
SR10019VA-1 Mouse IBA-1 Differentiation Reporter (pGreenZeo, Virus) >2 x 10^6 IFUs $662.00
- +
Contact Us

Overview

Overview

Monitor neurogenesis in real time With SBI’s line of pGreenZeo, pRedZeo, and pRedTK Differentation Reporter Vectors, you can monitor stem cell differentiation in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built differentiation reporters come as ready-to-package lentivector plasmids or ready-to-transduce pre-packaged lentivirus. The Mouse IBA-1 pGreenZeo Differentiation Reporter co-expresses dscGFP (destabilized copGFP, 2-hour half-life) and zeomycin resistance from the mouse IBA-1 promoter/enhancer elements, enabling visualization of neurogenesis using GFP fluorescence and selection for the desired cells using zeomycin.
  • Create stable neurogenesis-reporting cell lines
  • Monitor multiple lineages simultaneously
  • Track differentiation in live cells in real time
Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes. Choose the differentiation reporter that’s right for you Neural differentiation reporters
Catalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional names
SR1008PA/VA-1Macrophage, microgliaMouseCd68Cd68macrosialin, Scard1, gp110
SR10015PA/VA-1AstrocyteHumanGFAPGFAPGlial fibrillary acidic protein
SR10016PA/VA-1AstrocyteMouseGfapGfapGlial fibrillary acidic protein
SR10017PA/VA-1MicrogliaHumanCD11bITGAMintegrin, alpha M, CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A
SR10018PA/VA-1MicrogliaMouseEMR1Emr1EGF-like module containing, mucin-like, hormone receptor-like sequence 1, EGF-TM7, F4/80
SR10019PA/VA-1MicrogliaMouseIba-1Aif1allograft inflammatory factor 1 (Aif-1), ionized calcium binding adapter molecule 1 (Iba-1)
SR10020PA/VA-1Muller gliaMouseCd44Cd44HERMES, Ly-24, Pgp-1
SR10021PA/VA-1NeuronHumanBM88Cend1cell cycle exit and neuronal differentiation 1 (Cend1)
SR10022PA/VA-1NeuronMouseCamk2aCamk2aCaMK II; alpha-CaMKII
SR10023PA/VA-1NeuronMouseGAD67Gad1glutamic acid decarboxylase 1, EP10, GAD67, Gad-1
SR10024PA/VA-1NeuronRatNSEEno2enolase 2, gamma (Eno2), neuron-specific enolase (NSE)
SR10025PA/VA-1NeuronMouseTa1 a-tubulinTuba1atubulin, alpha 1A (Tuba1a), Tuba-1, Tuba1
SR10026PA/VA-1OligodendrocyteMouseMBPMbpmyelin basic protein (Mbp), myelin deficient; shiverer
SR10027PA/VA-1PhotoreceptorHumanOpsinOPN1SWopsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan)
SR10034PA/VA-1Neural Stem CellRatNestinNesNes
SR10035PA/VA-1Neural Stem CellHumanNestinNESNES
SR10041PA/VA-1NeuronHumanDoublecortinDCXDBCN, DC, LISX, SCLH, XLIS
SR10047PA/VA-1NeuronHumanMAP2MAP2MAP2A, MAP2B, MAP2C
SR10048PA/VA-1NeuronHumanFABP7FABP7B-FABP, BLBP, DKFZp547J2313, FABPB, MRG
Myogenic differentiation reporters
Catalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional names
SR10010PA/VA-1CardiomyocyteMouseActcActc1Cardiac muscle alpha actin 1
SR10011PA/VA-1CardiomyocyteHumanMLC-2vMYL2MYL2, MLC2
SR10012PA/VA-1CardiomyocyteHumanTNNT2TNNT2Troponin T type 2 (cardiac), TnTC, cTnT
SR10013PA/VA-1CardiomyocyteMouseTnnt2Tnnt2Tnt, cTnT
SR10014PA/VA-1Smooth muscle myocyteMouseSM22aTaglnTransgelin (Tagln), SM22, Sm22a
SR10049PA/VA-1CardiomyocyteHumanACTCACTC1ACTC, CMD1R
SR10050PA/VA-1Skeletal myocyteMouseMyogeninMyogmyo; MYF4
Hematopoietic differentiation reporters
Catalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional names
SR1004PA/VA-1B-cellHumanB29CD79BCD79b, Ig-beta, Igb, Igbeta
SR1005PA/VA-1B-cellMouseB29Cd79bCd79b
SR1006PA/VA-1CD8 T-cellMouseCD8Cd8aBB154331, Ly-2, Ly-35, Ly-B, Lyt-2
SR1007PA/VA-1ErythroidHumanHLA-DRaHLA-DRAHLA-DRA1, major histocompatibility complex, class II, DR alpha
SR1008PA/VA-1Macrophage, microgliaMouseCd68Cd68macrosialin, Scard1, gp110
SR1009PA/VA-1Pan T-cellHumanCD2CD2SRBC, T11
SR10032PA/VA-1LymphocyteHumanLCKLCKYT16, p56lck, pp58lck
Structural differentiation reporters
Catalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional names
SR1001PA/VA-1ChondrocyteMouseCol2a1Col2a1collagen, type II, alpha 1
SR1002PA/VA-1OsteoblastHumanSPP1SPP1secreted phosphoprotein 1 (SPP1), osteopontin (OPN)
SR1003PA/VA-1OsteoblastHumanOsteocalcinBGLAPbone gamma-carboxyglutamate (BGLAP), BGP, PMF1
SR10036PA/VA-1AdipocyteMouseALBPFabp4422/aP2, ALBP/Ap2, Ap2, Lbpl
SR10038PA/VA-1EpitheliumHumanKeratin 14KRT14CK14, EBS3, EBS4, K14, NFJ
Signaling differentiation reporters
Catalog #Target cell typeSpeciesPromoter/enhancer elementOfficial nameAdditional names
SR10028PA/VA-1Beta cellHumanInsulinINSinsulin
SR10037PA/VA-1IsletHumanNGN3NGN3neurogenin 3, Atoh5, Math4B, bHLHa7, neurog3
SR10039PA/VA-1IsletHumanPDX1PDX1IDX-1, IPF1, IUF1, MODY4, PDX-1, STF-1
SR10040PA/VA-1IsletMousePdx1Pdx1IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1

How It Works

Supporting Data

Supporting Data

See some of our differentiation reporters in action

SBI’s differentiation reporters are used in a number of papers. The data shown below are just one example (from Ravin R, Hoeppner DJ, Munno DM, Carmel L, Sullivan J, Levitt DL, Miller JL, Athaide C, Panchision DM, McKay RD. Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 2008 Dec 4; 3(6):670-80. PMID: 19041783)

Live imaging of neuronal differentiation

Figure 1. Live imaging of neuronal differentiation. Ravin, et al, used SBI’s Mouse IBA-1 pGreenFire Differentiation Reporter (Cat.# SR10016VA-1), which drives GFP expression from the glial fibrillary acidic protein promoter, to watch mouse neural stem cells differentiate into a network of mature neurons, oligodendrocytes, and astrocytes over the course of seven days. The periodic “flashes” seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The final photo taken after the network formation is shown below the video (color added). Among the network of neurons, only the astrocytes are bright green, demonstrating the specificity of SBI’s mouse IBA-1 pGreenFire Differentiation Reporter.

Simultaneously track multiple lineages from iPS and progenitor cells

Figure 2. Simultaneously track multiple lineages from iPS and progenitor cells.

See SBI’s differentiation reporters in action

Figure 3. Additional differentiation reporter data.

Citations