ExoELISA Complete Kit (CD9 Detection)

A sensitive ELISA assay for exosome quantitation based on the presence of CD9, a general exosome marker. Compatible with most biofluids.
  • Sensitive—detect as little as 500 µg protein equivalent
  • Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD9

Products

Overview

Overview

Sensitive, ELISA-based exosome quantitation

When you would like to use an ELISA-based method to find out how many exosomes you have and time is not limiting, choose one of SBI’s ExoELISA Complete Kits. Calibrated using NanoSight Analysis, the ExoELISA Complete Kit (CD9 Detection) enables calculation of exosome abundance based on the presence of CD9, a general exosome marker. Note that our newer ExoELISA-ULTRA Complete Kit (CD63 Detection) can provide faster exosome quantitation using significantly less sample.

  • Sensitive—detect as little as 500 µg protein equivalent
  • Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD9

How It Works

How It Works

Our ExoELISA Kits have all the reagents you need to run the ELISA—just add lysed exosome particles. The kits are compatible with exosomes isolated using most methods, including ExoQuick®, ExoQuick-TC®, or ultracentrifugation.

The ExoELISA assay uses a colorimetric, HRP activity-based readout using extra-sensitive TMB as the substrate.

The lysed exosome particles (and, thus, exosomal proteins) are directly immobilized onto the wells of the microtiter plate, and after binding, a blocking agent is added to prevent non-specific binding of the primary detection antibody, anti-CD9. Following addition of anti-CD9, a secondary antibody (goat anti-rabbit) linked to horseradish peroxidase (HRP) is also added to amplify the signal and increase assay sensitivity.

The amount of CD9 is measured via activity of the bound HRP-secondary antibody using a colorimetric assay with extra-sensitive TMB as the substrate. The accumulation of colored product is proportional to the amount of CD9 present in each well, and is measured using a microtiter plate reader at 450 nm absorbance.

Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying CD9 from the ExoELISA data.An example ExoELISA CD9 calibration curve using the included exosome standards.The number of exosome particles in the standards supplied with each ExoELISA Kit are quantified via NanoSight Analysis.

Supporting Data

Resources

Citations

  • Gandham, SK, Attarwala, HZ & Amiji, MM. (2022) Mathematical Modeling and Experimental Validation of Extracellular Vesicle-Mediated Tumor Suppressor MicroRNA Delivery and Propagation in Ovarian Cancer Cells. Molecular pharmaceutics. 1970 Jan 1;. PM ID: 36226722
  • Yoon, S, Bogdanov, K & Wallach, D. (2022) Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes. Cell death and differentiation. 1970 Jan 1;. PM ID: 34999730
  • Cilibrasi, C, et al. (2022) Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients. Biomedicines. 1970 Jan 1; 10(1). PM ID: 35052804
  • Melling, GE, et al. (2022) Confocal microscopy analysis reveals that only a small proportion of extracellular vesicles are successfully labelled with commonly utilised staining methods. Scientific reports. 1970 Jan 1; 12(1):262. PM ID: 34997141
  • Chetty, V, et al. (2022) Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell-Cell Communication in Cancer. Cancers. 1970 Jan 1; 14(9):2068. Link: Cancers
  • Hong, L, et al. (2022) Exosomal circular RNA hsa_circ_0006220, and hsa_circ_0001666 as biomarkers in the diagnosis of pancreatic cancer. Journal of clinical laboratory analysis. 1970 Jan 1;:e24447. PM ID: 35446993
  • Li, Y, et al. (2022) Mouse mesenchymal stem cell-derived exosomal miR-466f-3p reverses EMT process through inhibiting AKT/GSK3β pathway via c-MET in radiation-induced lung injury. Journal of experimental & clinical cancer research : CR. 1970 Jan 1; 41(1):128. PM ID: 35392967
  • Xiao, W, et al. (2022) Matrix stiffness mediates pancreatic cancer chemoresistance through induction of exosome hypersecretion in a cancer associated fibroblasts-tumor organoid biomimetic model. Matrix biology plus. 1970 Jan 1; 14:100111. PM ID: 35619988
  • Rodrigues, LJS. (2022) Characterization of extracellular vesicles from ascitic fluid in ovarian cancer patients. Thesis. 1970 Jan 1;. Link: Thesis
  • Hrdinova, T. (2022) Molecular events associated with resistance to tyrosine kinase inhibitors in leukemia cells.. Thesis. 1970 Jan 1;. Link: Thesis
  • Blommer, J, et al. (2022) Extracellular vesicle biomarkers for cognitive impairment in Parkinson’s disease. Brain : a journal of neurology. 1970 Jan 1;. PM ID: 35833836
  • Yu, HY, et al. (2022) Exosomes from PM 2.5-treated Human Bronchial Epithelial Cells Increase Lung Cancer Metastatic Potential. Biomedical and environmental sciences : BES. 1970 Jan 1; 35(6):473-484. PM ID: 35882407
  • Meliciano, AEL. (2022) Empowering the therapeutic potential of clinically expired platelet concentrates: a new source of extracellular vesicles. Thesis. 1970 Jan 1;. Link: Thesis
  • Jayathilaka, EHTT, et al. (2022) Isolation and characterization of plasma-derived exosomes from olive flounder (Paralichthys olivaceus) and their wound healing and regeneration activities. Fish & shellfish immunology. 1970 Jan 1; 128:196-205. PM ID: 35932983
  • Kim, CG, et al. (2022) Small RNA sequencing of small extracellular vesicles secreted by umbilical cord mesenchymal stem cells following replicative senescence. Genes & genomics. 1970 Jan 1;. PM ID: 35917089
  • Freitas, R. (2022) Avaliação do perfil de microRNAs exossomais em portadores de hipercolesterolemia familial. Universidade de São Paulo. 1970 Jan 1;. Link: Universidade de São Paulo
  • André-Grégoire, G, et al. (2022) Inhibition of the pseudokinase MLKL alters extracellular vesicle release and reduces tumor growth in glioblastoma. iScience. 1970 Jan 1; 25(10):105118. Link: iScience
  • Zhang, W, Zhang, J & Huang, H. (2022) Exosomes from adipose-derived stem cells inhibit inflammation and oxidative stress in LPS-acute kidney injury. Experimental cell research. 1970 Jan 1; 420(1):113332. PM ID: 36084668
  • Andreu, Z, et al. (2022) A Rapid, Convergent Approach to the Identification of Exosome Inhibitors in Breast Cancer Models. Nanotheranostics. 1970 Jan 1; 7(1):1-21. Link: Nanotheranostics
  • Kuhn, M, et al. (2022) IMP1/IGF2BP1 in human colorectal cancer extracellular vesicles. American journal of physiology. Gastrointestinal and liver physiology. 1970 Jan 1;. PM ID: 36194131

Products

Overview

Overview

Sensitive, ELISA-based exosome quantitation

When you would like to use an ELISA-based method to find out how many exosomes you have and time is not limiting, choose one of SBI’s ExoELISA Complete Kits. Calibrated using NanoSight Analysis, the ExoELISA Complete Kit (CD9 Detection) enables calculation of exosome abundance based on the presence of CD9, a general exosome marker. Note that our newer ExoELISA-ULTRA Complete Kit (CD63 Detection) can provide faster exosome quantitation using significantly less sample.

  • Sensitive—detect as little as 500 µg protein equivalent
  • Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD9

How It Works

How It Works

Our ExoELISA Kits have all the reagents you need to run the ELISA—just add lysed exosome particles. The kits are compatible with exosomes isolated using most methods, including ExoQuick®, ExoQuick-TC®, or ultracentrifugation.

The ExoELISA assay uses a colorimetric, HRP activity-based readout using extra-sensitive TMB as the substrate.

The lysed exosome particles (and, thus, exosomal proteins) are directly immobilized onto the wells of the microtiter plate, and after binding, a blocking agent is added to prevent non-specific binding of the primary detection antibody, anti-CD9. Following addition of anti-CD9, a secondary antibody (goat anti-rabbit) linked to horseradish peroxidase (HRP) is also added to amplify the signal and increase assay sensitivity.

The amount of CD9 is measured via activity of the bound HRP-secondary antibody using a colorimetric assay with extra-sensitive TMB as the substrate. The accumulation of colored product is proportional to the amount of CD9 present in each well, and is measured using a microtiter plate reader at 450 nm absorbance.

Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying CD9 from the ExoELISA data.An example ExoELISA CD9 calibration curve using the included exosome standards.The number of exosome particles in the standards supplied with each ExoELISA Kit are quantified via NanoSight Analysis.

Supporting Data

Citations

  • Gandham, SK, Attarwala, HZ & Amiji, MM. (2022) Mathematical Modeling and Experimental Validation of Extracellular Vesicle-Mediated Tumor Suppressor MicroRNA Delivery and Propagation in Ovarian Cancer Cells. Molecular pharmaceutics. 1970 Jan 1;. PM ID: 36226722
  • Yoon, S, Bogdanov, K & Wallach, D. (2022) Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes. Cell death and differentiation. 1970 Jan 1;. PM ID: 34999730
  • Cilibrasi, C, et al. (2022) Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients. Biomedicines. 1970 Jan 1; 10(1). PM ID: 35052804
  • Melling, GE, et al. (2022) Confocal microscopy analysis reveals that only a small proportion of extracellular vesicles are successfully labelled with commonly utilised staining methods. Scientific reports. 1970 Jan 1; 12(1):262. PM ID: 34997141
  • Chetty, V, et al. (2022) Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell-Cell Communication in Cancer. Cancers. 1970 Jan 1; 14(9):2068. Link: Cancers
  • Hong, L, et al. (2022) Exosomal circular RNA hsa_circ_0006220, and hsa_circ_0001666 as biomarkers in the diagnosis of pancreatic cancer. Journal of clinical laboratory analysis. 1970 Jan 1;:e24447. PM ID: 35446993
  • Li, Y, et al. (2022) Mouse mesenchymal stem cell-derived exosomal miR-466f-3p reverses EMT process through inhibiting AKT/GSK3β pathway via c-MET in radiation-induced lung injury. Journal of experimental & clinical cancer research : CR. 1970 Jan 1; 41(1):128. PM ID: 35392967
  • Xiao, W, et al. (2022) Matrix stiffness mediates pancreatic cancer chemoresistance through induction of exosome hypersecretion in a cancer associated fibroblasts-tumor organoid biomimetic model. Matrix biology plus. 1970 Jan 1; 14:100111. PM ID: 35619988
  • Rodrigues, LJS. (2022) Characterization of extracellular vesicles from ascitic fluid in ovarian cancer patients. Thesis. 1970 Jan 1;. Link: Thesis
  • Hrdinova, T. (2022) Molecular events associated with resistance to tyrosine kinase inhibitors in leukemia cells.. Thesis. 1970 Jan 1;. Link: Thesis
  • Blommer, J, et al. (2022) Extracellular vesicle biomarkers for cognitive impairment in Parkinson’s disease. Brain : a journal of neurology. 1970 Jan 1;. PM ID: 35833836
  • Yu, HY, et al. (2022) Exosomes from PM 2.5-treated Human Bronchial Epithelial Cells Increase Lung Cancer Metastatic Potential. Biomedical and environmental sciences : BES. 1970 Jan 1; 35(6):473-484. PM ID: 35882407
  • Meliciano, AEL. (2022) Empowering the therapeutic potential of clinically expired platelet concentrates: a new source of extracellular vesicles. Thesis. 1970 Jan 1;. Link: Thesis
  • Jayathilaka, EHTT, et al. (2022) Isolation and characterization of plasma-derived exosomes from olive flounder (Paralichthys olivaceus) and their wound healing and regeneration activities. Fish & shellfish immunology. 1970 Jan 1; 128:196-205. PM ID: 35932983
  • Kim, CG, et al. (2022) Small RNA sequencing of small extracellular vesicles secreted by umbilical cord mesenchymal stem cells following replicative senescence. Genes & genomics. 1970 Jan 1;. PM ID: 35917089
  • Freitas, R. (2022) Avaliação do perfil de microRNAs exossomais em portadores de hipercolesterolemia familial. Universidade de São Paulo. 1970 Jan 1;. Link: Universidade de São Paulo
  • André-Grégoire, G, et al. (2022) Inhibition of the pseudokinase MLKL alters extracellular vesicle release and reduces tumor growth in glioblastoma. iScience. 1970 Jan 1; 25(10):105118. Link: iScience
  • Zhang, W, Zhang, J & Huang, H. (2022) Exosomes from adipose-derived stem cells inhibit inflammation and oxidative stress in LPS-acute kidney injury. Experimental cell research. 1970 Jan 1; 420(1):113332. PM ID: 36084668
  • Andreu, Z, et al. (2022) A Rapid, Convergent Approach to the Identification of Exosome Inhibitors in Breast Cancer Models. Nanotheranostics. 1970 Jan 1; 7(1):1-21. Link: Nanotheranostics
  • Kuhn, M, et al. (2022) IMP1/IGF2BP1 in human colorectal cancer extracellular vesicles. American journal of physiology. Gastrointestinal and liver physiology. 1970 Jan 1;. PM ID: 36194131