Cytosol Cyto-TracerTM, pCT-Cyto-GFPLuc (MSCV)
Study cellular processes and physiology with this Cyto-Tracer that localizes copGFP and luciferase to the cytosol – reporter expression is driven by MSCV
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CYTO121-VB-1 | pCT-Cyto-GFPLuc (pMSCV, Cytosol, GFP+Luciferase Untagged; pre-packaged virus) | 10 µg | $715 |
|
||||
| CYTO121-PB-1 | pCT-Cyto-GFPLuc (pMSCV, Cytosol, GFP+Luciferase Untagged) | 10 µg | $620 |
|
||||
Overview
Overview
Easily study the dynamics of cellular processes with SBI’s Cyto-TracersTM
Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Cytosol Cyto-Tracer, pCT-Cyto-GFPLuc (MSCV), co-expresses an untagged copGFP and luciferase, both of which naturally localize to the cytosol. copGFP and luciferase are co-expressed from the MSCV promoter for strong expression in hematopoietic and stem cells.
- Stable lentivector-based system
- Great for creating stable reporter cell lines
- Ideal for co-localization studies
- Monitor cellular dynamics and functional studies in real time
- Label cells with GFP for a broad range of studies
Choose the Cyto-Tracer that’s right for you
| Promoter (Cat.#) | Cyto-Tracer | Target Location | Peptide Tag |
|---|---|---|---|
| CMV (CYTO100-PA-1/CYTO100-VA-1) MSCV (CYTO100-PB-1/CYTO100-VB-1) | pCT-Mem-GFP | Plasma Membrane | Neuromodulin |
| CMV (CYTO101-PA-1/CYTO101-VA-1) MSCV (CYTO101-PB-1/CYTO101-VB-1) | pCT-InnerMem-GFP | Inner Leaflet of Membrane | Farnesylation signal |
| CMV (CYTO102-PA-1/CYTO102-VA-1) MSCV (CYTO102-PB-1/CYTO102-VB-1) | pCT-Mito-GFP | Mitochondria | Cytochrome C oxidase VIII |
| CMV (CYTO103-PA-1/CYTO103-VA-1) | pCT-ER-GFP | Endoplasmic Reticulum | ER targeting consensus |
| CMV (CYTO104-PA-1/CYTO104-VA-1) | pCT-Golgi-GFP | Golgi Apparatus | Galactosyltrasferase |
| CMV (CYTO105-PA-1/CYTO105-VA-1) | pCT-Secretory-GFP | ER-Golgi Vesicles | Secretory consensus peptide |
| CMV (CYTO106-PA-1/CYTO106-VA-1) | pCT-Lyso-GFP | Lysosome | Cathepsin B |
| CMV (CYTO107-PA-1/CYTO107-VA-1) | pCT-Endo-GFP | Endosome | RhoB |
| CMV (CYTO107R-PA-1/CYTO107R-VA-1) | pCT-Endo-RFP | Endosome | RhoB |
| CMV (CYTO108-PA-1/CYTO108-VA-1) MSCV (CYTO108-PB-1/CYTO108-VB-1) | pCT-Pero-GFP | Peroxisome | Peroxisomal consensus signal |
| CMV (CYTO109-PA-1/CYTO109-VA-1) | pCT-Autophago-GFP | Autophagosome | LC3 |
| CMV (CYTO109R-PA-1/CYTO109R-VA-1) | pCT-Autophago-RFP | Autophagosome | LC3 |
| CMV (CYTO120-PA-1/CYTO120-VA-1) | pCT-CD63-GFP | Exosome/Secretory | CD63 Tetraspanin |
| CMV (CYTO120R-PA-1/CYTO120R-VA-1) | pCT-CD63-RFP | Exosome/Secretory | CD63 Tetraspanin |
| CMV (CYTO122-PA-1/CYTO122-VA-1) | pCT-CD9-GFP | Exosome/Secretory | CD9 Tetraspanin |
| CMV (CYTO123-PA-1/CYTO123-VA-1) | pCT-CD9-RFP | Exosome/Secretory | CD9 Tetraspanin |
| CMV (CYTO124-PA-1/CYTO124-VA-1) | pCT-CD81-GFP | Exosome/Secretory | CD81 Tetraspanin |
| CMV (CYTO125-PA-1/CYTO125-VA-1) | pCT-CD81-RFP | Exosome/Secretory | CD81 Tetraspanin |
| CMV (CYTO110-PA-1/CYTO110-VA-1) MSCV (CYTO110-PB-1/CYTO110-VB-1) | pCT-Actin-GFP | Cytoskeleton | β-actin |
| CMV (CYTO111-PA-1/CYTO111-VA-1) MSCV (CYTO111-PB-1/CYTO111-VB-1) | pCT-Tublin-GFP | Cytoskeleton | α-tublin |
| CMV (CYTO111R-PA-1/CYTO111R-VA-1) | pCT-Tublin-RFP | Cytoskeleton | α-tublin |
| CMV (CYTO112-PA-1/CYTO112-VA-1) | pCT-MAP4-GFP | Microtubule | Microtubule associated protein 4 (MAP4) |
| CMV (CYTO113-PA-1/CYTO113-VA-1) MSCV (CYTO113-PB-1/CYTO113-VB-1) | pCT-H2B-GFP | Nucleus | H2B |
| CMV (CYTO114-PA-1/CYTO114-VA-1) | pCT-Apoptosis-Luc | Caspase 3/7 activation | Cyclic inactive luciferase |
| CMV (CYTO115-PA-1/CYTO115-VA-1) | pCT-GFP-BAX | Cytosol to Mitochondria | BAX |
| CMV (CYTO116-PA-1/CYTO116-VA-1) | pCT- Catenin-GFP | Cytosol to Nucleus/Cell Membrane | β-Catenin activation |
| CMV (CYTO117-PA-1/CYTO117-VA-1) | pCT-CMV-PSD95-GFP | Dendrite Membranes | PSD-95 |
| CMV (CYTO118-PA-1/CYTO118-VA-1) MSCV (CYTO118-PB-1/CYTO118-VB-1) | pCT-Cyto-GFP | Cytosolic Compartment | GFP-Untagged |
| CMV (CYTO119-PA-1/CYTO119-VA-1) | pCT-Cyto-RFP | Cytosolic Compartment | RFP-Untagged |
| MSCV (CYTO121-PB-1/CYTO121-VB-1) | pCT-Cyto-GFpLuc | GFP and Luciferase Control | GFP and Luciferase (Untagged) |
How It Works
How It Works
Visualizing the cell with Cyto-Tracers
SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location.
Supporting Data
Supporting Data
See some of our Cyto-Tracers in action
FAQs
Resources
Related Products
Citations
-
Kessler, M, et al. (2019) Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation. Nat Commun. 2019; 10(1):1194. PM ID: 30886143
-
Gonzalez-King, H, et al. (2017) Hypoxia Inducible Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes. Stem Cells. 2017; 35(7):1747-1759. PM ID: 28376567
-
Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival. Carcinogenesis. 2017; 38(6):592-603. PM ID: 28402394
-
Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular. Thesis. 2017;. Link: Thesis
-
Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J. Biol. Chem.. 2017; 292(6):2470-2484. PM ID: 28003368
-
Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes. Tumour Biol.. 2016; 37(1):313-21. PM ID: 26211004
-
Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation. Nat Commun. 2015; 6:7506. PM ID: 26103923
-
Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway. Thesis. 2015;. Link: Thesis
-
Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie. Thesis. 2015;. Link: Thesis
-
VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI. Thesis. 2015;. Link: Thesis
-
Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J. Biol. Chem.. 2014; 289(47):32526-37. PM ID: 25261472
-
Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. Nat Commun. 2014; 5:5513. PM ID: 25423885
-
Sato, Y, et al. (2014) MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nat Commun. 2014; 5:5266. PM ID: 25366663
Cytosol Cyto-TracerTM, pCT-Cyto-GFPLuc (MSCV)
$620.00
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CYTO121-VB-1 | pCT-Cyto-GFPLuc (pMSCV, Cytosol, GFP+Luciferase Untagged; pre-packaged virus) | 10 µg | $715 |
|
||||
| CYTO121-PB-1 | pCT-Cyto-GFPLuc (pMSCV, Cytosol, GFP+Luciferase Untagged) | 10 µg | $620 |
|
||||
Overview
Overview
Easily study the dynamics of cellular processes with SBI’s Cyto-TracersTM
Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Cytosol Cyto-Tracer, pCT-Cyto-GFPLuc (MSCV), co-expresses an untagged copGFP and luciferase, both of which naturally localize to the cytosol. copGFP and luciferase are co-expressed from the MSCV promoter for strong expression in hematopoietic and stem cells.
- Stable lentivector-based system
- Great for creating stable reporter cell lines
- Ideal for co-localization studies
- Monitor cellular dynamics and functional studies in real time
- Label cells with GFP for a broad range of studies
Choose the Cyto-Tracer that’s right for you
| Promoter (Cat.#) | Cyto-Tracer | Target Location | Peptide Tag |
|---|---|---|---|
| CMV (CYTO100-PA-1/CYTO100-VA-1) MSCV (CYTO100-PB-1/CYTO100-VB-1) | pCT-Mem-GFP | Plasma Membrane | Neuromodulin |
| CMV (CYTO101-PA-1/CYTO101-VA-1) MSCV (CYTO101-PB-1/CYTO101-VB-1) | pCT-InnerMem-GFP | Inner Leaflet of Membrane | Farnesylation signal |
| CMV (CYTO102-PA-1/CYTO102-VA-1) MSCV (CYTO102-PB-1/CYTO102-VB-1) | pCT-Mito-GFP | Mitochondria | Cytochrome C oxidase VIII |
| CMV (CYTO103-PA-1/CYTO103-VA-1) | pCT-ER-GFP | Endoplasmic Reticulum | ER targeting consensus |
| CMV (CYTO104-PA-1/CYTO104-VA-1) | pCT-Golgi-GFP | Golgi Apparatus | Galactosyltrasferase |
| CMV (CYTO105-PA-1/CYTO105-VA-1) | pCT-Secretory-GFP | ER-Golgi Vesicles | Secretory consensus peptide |
| CMV (CYTO106-PA-1/CYTO106-VA-1) | pCT-Lyso-GFP | Lysosome | Cathepsin B |
| CMV (CYTO107-PA-1/CYTO107-VA-1) | pCT-Endo-GFP | Endosome | RhoB |
| CMV (CYTO107R-PA-1/CYTO107R-VA-1) | pCT-Endo-RFP | Endosome | RhoB |
| CMV (CYTO108-PA-1/CYTO108-VA-1) MSCV (CYTO108-PB-1/CYTO108-VB-1) | pCT-Pero-GFP | Peroxisome | Peroxisomal consensus signal |
| CMV (CYTO109-PA-1/CYTO109-VA-1) | pCT-Autophago-GFP | Autophagosome | LC3 |
| CMV (CYTO109R-PA-1/CYTO109R-VA-1) | pCT-Autophago-RFP | Autophagosome | LC3 |
| CMV (CYTO120-PA-1/CYTO120-VA-1) | pCT-CD63-GFP | Exosome/Secretory | CD63 Tetraspanin |
| CMV (CYTO120R-PA-1/CYTO120R-VA-1) | pCT-CD63-RFP | Exosome/Secretory | CD63 Tetraspanin |
| CMV (CYTO122-PA-1/CYTO122-VA-1) | pCT-CD9-GFP | Exosome/Secretory | CD9 Tetraspanin |
| CMV (CYTO123-PA-1/CYTO123-VA-1) | pCT-CD9-RFP | Exosome/Secretory | CD9 Tetraspanin |
| CMV (CYTO124-PA-1/CYTO124-VA-1) | pCT-CD81-GFP | Exosome/Secretory | CD81 Tetraspanin |
| CMV (CYTO125-PA-1/CYTO125-VA-1) | pCT-CD81-RFP | Exosome/Secretory | CD81 Tetraspanin |
| CMV (CYTO110-PA-1/CYTO110-VA-1) MSCV (CYTO110-PB-1/CYTO110-VB-1) | pCT-Actin-GFP | Cytoskeleton | β-actin |
| CMV (CYTO111-PA-1/CYTO111-VA-1) MSCV (CYTO111-PB-1/CYTO111-VB-1) | pCT-Tublin-GFP | Cytoskeleton | α-tublin |
| CMV (CYTO111R-PA-1/CYTO111R-VA-1) | pCT-Tublin-RFP | Cytoskeleton | α-tublin |
| CMV (CYTO112-PA-1/CYTO112-VA-1) | pCT-MAP4-GFP | Microtubule | Microtubule associated protein 4 (MAP4) |
| CMV (CYTO113-PA-1/CYTO113-VA-1) MSCV (CYTO113-PB-1/CYTO113-VB-1) | pCT-H2B-GFP | Nucleus | H2B |
| CMV (CYTO114-PA-1/CYTO114-VA-1) | pCT-Apoptosis-Luc | Caspase 3/7 activation | Cyclic inactive luciferase |
| CMV (CYTO115-PA-1/CYTO115-VA-1) | pCT-GFP-BAX | Cytosol to Mitochondria | BAX |
| CMV (CYTO116-PA-1/CYTO116-VA-1) | pCT- Catenin-GFP | Cytosol to Nucleus/Cell Membrane | β-Catenin activation |
| CMV (CYTO117-PA-1/CYTO117-VA-1) | pCT-CMV-PSD95-GFP | Dendrite Membranes | PSD-95 |
| CMV (CYTO118-PA-1/CYTO118-VA-1) MSCV (CYTO118-PB-1/CYTO118-VB-1) | pCT-Cyto-GFP | Cytosolic Compartment | GFP-Untagged |
| CMV (CYTO119-PA-1/CYTO119-VA-1) | pCT-Cyto-RFP | Cytosolic Compartment | RFP-Untagged |
| MSCV (CYTO121-PB-1/CYTO121-VB-1) | pCT-Cyto-GFpLuc | GFP and Luciferase Control | GFP and Luciferase (Untagged) |
How It Works
How It Works
Visualizing the cell with Cyto-Tracers
SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location.
Supporting Data
Supporting Data
See some of our Cyto-Tracers in action
FAQs
Citations
-
Kessler, M, et al. (2019) Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation. Nat Commun. 2019; 10(1):1194. PM ID: 30886143
-
Gonzalez-King, H, et al. (2017) Hypoxia Inducible Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes. Stem Cells. 2017; 35(7):1747-1759. PM ID: 28376567
-
Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival. Carcinogenesis. 2017; 38(6):592-603. PM ID: 28402394
-
Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular. Thesis. 2017;. Link: Thesis
-
Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J. Biol. Chem.. 2017; 292(6):2470-2484. PM ID: 28003368
-
Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes. Tumour Biol.. 2016; 37(1):313-21. PM ID: 26211004
-
Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation. Nat Commun. 2015; 6:7506. PM ID: 26103923
-
Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway. Thesis. 2015;. Link: Thesis
-
Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie. Thesis. 2015;. Link: Thesis
-
VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI. Thesis. 2015;. Link: Thesis
-
Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J. Biol. Chem.. 2014; 289(47):32526-37. PM ID: 25261472
-
Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. Nat Commun. 2014; 5:5513. PM ID: 25423885
-
Sato, Y, et al. (2014) MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nat Commun. 2014; 5:5266. PM ID: 25366663