Exo-Fect™ Exosome Transfection Kit
- Easy-to-use with a fast and straightforward loading protocol
- Introduces a wide range of biomolecules directly into isolated exosomes:
- RNAs, including siRNAs, miRNAs, and mRNAs
- DNAs, including plasmids
- Metabolites and other small molecules
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXFT10A-1 | Exo-Fect Exosome Transfection Kit | 10 Reactions | $247 |
|
||||
EXFT20A-1 | Exo-Fect Exosome Transfection Kit | 20 Reactions | $445 |
|
Overview
Overview
Putting exosomes to work: Load your cargo directly into isolated exosomes With Exo-Fect, you can turn isolated exosomes into cargo delivery vehicles that introduce RNAs, DNAs (including plasmids), and even small molecules into recipient cells. Simply combine isolated exosomes with Exo-Fect and your desired cargo, and in less than an hour your loaded exosomes will be ready to add to recipient cells.- Easy-to-use with a fast and straightforward loading protocol
- Introduces a wide range of biomolecules directly into isolated exosomes:
- RNAs, including siRNAs, miRNAs, and mRNAs
- DNAs, including plasmids
- Metabolites and other small molecules
- Enables non-viral transduction and stable cell line creation
- Provides an alternative gene delivery method for hard-to-transfect cells
How It Works
How It Works
Quickly and easily load cargo into isolated exosomes
Simply combine the isolated exosomes with Exo-Fect Reagent and cargo, perform two incubations for a total of 40 minutes, and then pellet your exosomes. The Kit comes with all of the reagents you need to load cargo into exosomes and concentrate them for delivery to target cells. The protocol takes less than an hour and is highly efficient at loading cargo into exosomes for transport and delivery.
Exo-Fect is also compatible with the EV-Entry Reagent, which maximizes cargo uptake by recipient cells.
Supporting Data
Supporting Data
See how Exo-Fect can be used to create cargo-delivering exosomes
Exo-Fect efficiently loads small RNAs into exosomes for delivery to recipient cells
Exo-Fect efficiently loads mRNAs and DNA plasmids into exosomes for delivery to recipient cells
Exo-Fect efficiently loads small molecules into exosomes for delivery to recipient cells
FAQs
Resources
Related Products
Citations
-
Zhang, Y, et al. (2024) Islet-resident macrophage-derived miR-155 promotes β cell decompensation via targeting PDX1. iScience. 2024; 27(4):109540. Link: iScience
-
Mao, Y, et al. (2024) Hypoxia induced exosomal Circ-ZNF609 promotes pre-metastatic niche formation and cancer progression via miR-150-5p/VEGFA and HuR/ZO-1 axes in esophageal squamous cell carcinoma. Cell death discovery. 2024; 10(1):133. PM ID: 38472174
-
Guan, T, et al. (2024) Oxidized SOD1 accelerates cellular senescence in neural stem cells. Stem cell research & therapy. 2024; 15(1):55. PM ID: 38414053
-
Xu, K, et al. (2024) Elevated extracellular matrix protein 1 in circulating extracellular vesicles supports breast cancer progression under obesity conditions. Nature communications. 2024; 15(1):1685. PM ID: 38402239
-
Zhang, L, et al. (2024) Engineering M2 type macrophage-derived exosomes for autoimmune hepatitis immunotherapy via loading siRIPK3. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2024; 171:116161. PM ID: 38244330
-
Heo, J & Ryu, J. (2024) Exosomal noncoding RNA: a potential therapy for retinal vascular diseases. Molecular Therapy - Nucleic Acids. 2024;:102128. Link: Molecular Therapy - Nucleic Acids
-
Zhao, W, et al. (2023) Accelerating corneal wound healing using exosome-mediated targeting of NF-κB c-Rel. Inflammation and regeneration. 2023; 43(1):6. PM ID: 36703231
-
Lang, HL, et al. (2023) Small extracellular vesicles secreted by induced pluripotent stem cell-derived mesenchymal stem cells improve postoperative cognitive dysfunction in mice with diabetes. Neural regeneration research. 2023; 18(3):609-617. PM ID: 36018185
-
Lin, W, et al. (2023) Tumor-intrinsic YTHDF1 drives immune evasion and resistance to immune checkpoint inhibitors via promoting MHC-I degradation. Nature communications. 2023; 14(1):265. PM ID: 36650153
-
Lu, Y, et al. (2023) Exosome-Based Carrier for RNA Delivery: Progress and Challenges. Pharmaceutics. 2023; 15(2). PM ID: 36839920
-
Ellipilli, S, et al. (2023) Ligand-displaying-exosomes using RNA nanotechnology for targeted delivery of multi-specific drugs for liver cancer regression. Nanomedicine : nanotechnology, biology, and medicine. 2023; 50:102667. PM ID: 36948369
-
Zheng, Z, et al. (2023) Acinous cell AR42J-derived exosome miR125b-5p promotes acute pancreatitis exacerbation by inhibiting M2 macrophage polarization via PI3K/AKT signaling pathway. World Journal of Gastrointestinal Surgery. 2023; 15(4):600-620. Link: World Journal of Gastrointestinal Surgery
-
Hao, L, et al. (2023) Bone targeting miR-26a loaded exosome-mimetics for bone regeneration therapy by activating Wnt signaling pathway. Chemical Engineering Journal. 2023; 471:144594. Link: Chemical Engineering Journal
-
Chen, Y, et al. (2023) Sertoli cell-derived extracellular vesicles traverse the blood-testis barrier and deliver miR-24-3p inhibitor into germ cells improving sperm mobility. Journal of controlled release : official journal of the Controlled Release Society. 2023; 362:58-69. PM ID: 37595666
-
Tu, W, et al. (2023) Effective delivery of miR-511-3p with mannose-decorated exosomes with RNA nanoparticles confers protection against asthma. Journal of controlled release : official journal of the Controlled Release Society. 2023; 365:602-616. PM ID: 37996055
-
Feng, T, et al. (2023) Regulation of hepatic lipid metabolism by intestine epithelium-derived exosomes. Life Metabolism. 2023;. Link: Life Metabolism
-
Sun, W, et al. (2023) Vitamin D receptor-deficient keratinocytes-derived exosomal miR-4505 promotes the macrophage polarization towards the M1 phenotype. PeerJ. 2023; 11:e15798. PM ID: 37554338
-
Xiao, Y, et al. (2023) Thermos-responsive hydrogel system encapsulated engineered exosomes attenuate inflammation and oxidative damage in acute spinal cord injury. Frontiers in bioengineering and biotechnology. 2023; 11:1216878. PM ID: 37614633
-
Xie, W, et al. (2023) Inhibition of osteosarcoma by European Mistletoe derived val-miR218. Extracellular Vesicles and Circulating Nucleic Acids. 2023; 4(2):306-322. Link: Extracellular Vesicles and Circulating Nucleic Acids
-
Wu, Y, et al. (2022) Osteoclast-derived extracellular miR-106a-5p promotes osteogenic differentiation and facilitates bone defect healing. Cellular Signalling. 2022;:110549. Link: Cellular Signalling
- See More
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXFT10A-1 | Exo-Fect Exosome Transfection Kit | 10 Reactions | $247 |
|
||||
EXFT20A-1 | Exo-Fect Exosome Transfection Kit | 20 Reactions | $445 |
|
Overview
Overview
Putting exosomes to work: Load your cargo directly into isolated exosomes With Exo-Fect, you can turn isolated exosomes into cargo delivery vehicles that introduce RNAs, DNAs (including plasmids), and even small molecules into recipient cells. Simply combine isolated exosomes with Exo-Fect and your desired cargo, and in less than an hour your loaded exosomes will be ready to add to recipient cells.- Easy-to-use with a fast and straightforward loading protocol
- Introduces a wide range of biomolecules directly into isolated exosomes:
- RNAs, including siRNAs, miRNAs, and mRNAs
- DNAs, including plasmids
- Metabolites and other small molecules
- Enables non-viral transduction and stable cell line creation
- Provides an alternative gene delivery method for hard-to-transfect cells
How It Works
How It Works
Quickly and easily load cargo into isolated exosomes
Simply combine the isolated exosomes with Exo-Fect Reagent and cargo, perform two incubations for a total of 40 minutes, and then pellet your exosomes. The Kit comes with all of the reagents you need to load cargo into exosomes and concentrate them for delivery to target cells. The protocol takes less than an hour and is highly efficient at loading cargo into exosomes for transport and delivery.
Exo-Fect is also compatible with the EV-Entry Reagent, which maximizes cargo uptake by recipient cells.
Supporting Data
Supporting Data
See how Exo-Fect can be used to create cargo-delivering exosomes
Exo-Fect efficiently loads small RNAs into exosomes for delivery to recipient cells
Exo-Fect efficiently loads mRNAs and DNA plasmids into exosomes for delivery to recipient cells
Exo-Fect efficiently loads small molecules into exosomes for delivery to recipient cells
FAQs
Citations
-
Zhang, Y, et al. (2024) Islet-resident macrophage-derived miR-155 promotes β cell decompensation via targeting PDX1. iScience. 2024; 27(4):109540. Link: iScience
-
Mao, Y, et al. (2024) Hypoxia induced exosomal Circ-ZNF609 promotes pre-metastatic niche formation and cancer progression via miR-150-5p/VEGFA and HuR/ZO-1 axes in esophageal squamous cell carcinoma. Cell death discovery. 2024; 10(1):133. PM ID: 38472174
-
Guan, T, et al. (2024) Oxidized SOD1 accelerates cellular senescence in neural stem cells. Stem cell research & therapy. 2024; 15(1):55. PM ID: 38414053
-
Xu, K, et al. (2024) Elevated extracellular matrix protein 1 in circulating extracellular vesicles supports breast cancer progression under obesity conditions. Nature communications. 2024; 15(1):1685. PM ID: 38402239
-
Zhang, L, et al. (2024) Engineering M2 type macrophage-derived exosomes for autoimmune hepatitis immunotherapy via loading siRIPK3. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2024; 171:116161. PM ID: 38244330
-
Heo, J & Ryu, J. (2024) Exosomal noncoding RNA: a potential therapy for retinal vascular diseases. Molecular Therapy - Nucleic Acids. 2024;:102128. Link: Molecular Therapy - Nucleic Acids
-
Zhao, W, et al. (2023) Accelerating corneal wound healing using exosome-mediated targeting of NF-κB c-Rel. Inflammation and regeneration. 2023; 43(1):6. PM ID: 36703231
-
Lang, HL, et al. (2023) Small extracellular vesicles secreted by induced pluripotent stem cell-derived mesenchymal stem cells improve postoperative cognitive dysfunction in mice with diabetes. Neural regeneration research. 2023; 18(3):609-617. PM ID: 36018185
-
Lin, W, et al. (2023) Tumor-intrinsic YTHDF1 drives immune evasion and resistance to immune checkpoint inhibitors via promoting MHC-I degradation. Nature communications. 2023; 14(1):265. PM ID: 36650153
-
Lu, Y, et al. (2023) Exosome-Based Carrier for RNA Delivery: Progress and Challenges. Pharmaceutics. 2023; 15(2). PM ID: 36839920
-
Ellipilli, S, et al. (2023) Ligand-displaying-exosomes using RNA nanotechnology for targeted delivery of multi-specific drugs for liver cancer regression. Nanomedicine : nanotechnology, biology, and medicine. 2023; 50:102667. PM ID: 36948369
-
Zheng, Z, et al. (2023) Acinous cell AR42J-derived exosome miR125b-5p promotes acute pancreatitis exacerbation by inhibiting M2 macrophage polarization via PI3K/AKT signaling pathway. World Journal of Gastrointestinal Surgery. 2023; 15(4):600-620. Link: World Journal of Gastrointestinal Surgery
-
Hao, L, et al. (2023) Bone targeting miR-26a loaded exosome-mimetics for bone regeneration therapy by activating Wnt signaling pathway. Chemical Engineering Journal. 2023; 471:144594. Link: Chemical Engineering Journal
-
Chen, Y, et al. (2023) Sertoli cell-derived extracellular vesicles traverse the blood-testis barrier and deliver miR-24-3p inhibitor into germ cells improving sperm mobility. Journal of controlled release : official journal of the Controlled Release Society. 2023; 362:58-69. PM ID: 37595666
-
Tu, W, et al. (2023) Effective delivery of miR-511-3p with mannose-decorated exosomes with RNA nanoparticles confers protection against asthma. Journal of controlled release : official journal of the Controlled Release Society. 2023; 365:602-616. PM ID: 37996055
-
Feng, T, et al. (2023) Regulation of hepatic lipid metabolism by intestine epithelium-derived exosomes. Life Metabolism. 2023;. Link: Life Metabolism
-
Sun, W, et al. (2023) Vitamin D receptor-deficient keratinocytes-derived exosomal miR-4505 promotes the macrophage polarization towards the M1 phenotype. PeerJ. 2023; 11:e15798. PM ID: 37554338
-
Xiao, Y, et al. (2023) Thermos-responsive hydrogel system encapsulated engineered exosomes attenuate inflammation and oxidative damage in acute spinal cord injury. Frontiers in bioengineering and biotechnology. 2023; 11:1216878. PM ID: 37614633
-
Xie, W, et al. (2023) Inhibition of osteosarcoma by European Mistletoe derived val-miR218. Extracellular Vesicles and Circulating Nucleic Acids. 2023; 4(2):306-322. Link: Extracellular Vesicles and Circulating Nucleic Acids
-
Wu, Y, et al. (2022) Osteoclast-derived extracellular miR-106a-5p promotes osteogenic differentiation and facilitates bone defect healing. Cellular Signalling. 2022;:110549. Link: Cellular Signalling
- See More