miRZipTM Pooled Anti-miRNA Virus Library

Efficiently conduct high-throughput phenotypic screens by quickly building stable cell lines where a specific miR is knocked down.

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miRZip pooled anti-miR virus library (Pre-packaged) >10^7 IFUs/aliquot

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miRZip pooled anti-miR virus library (Pre-packaged) >10^7 IFUs/aliquot

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1 Virus Aliquot
MZIPPLVA-1
$ 1004
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1-888-266-5066

Overview

Simplifying high-throughput phenotypic screening for miR function

Take your miR studies to the next level with SBI’s pooled miRZipTM anti-miRNA Lentivirus Library. Quickly and efficiently create stable miR knockdowns in the cell line of your choice for high-throughput phenotypic screening. SBI’s miRZip constructs leverage our powerful and well-regarded third-generation lentivector technology to deliver short hairpin RNAs (shRNA) that, after processing, preferentially produce an anti-sense miRNA. The hairpin is rationally designed to be asymmetric, ensuring that the sense strand does not contain the endogenous miRNA sequence and enabling accumulation of the anti-miRNA. The result is robust derepression of the transcripts targeted by the microRNA being “zipped,” and elevation of the corresponding protein levels.

SBI’s miRZip Lentivectors are designed for efficient production of anti-miRNAs

Study miRs with SBI’s powerful miRZip technology:

  • Stable and permanent anti-microRNA expression from a constitutive H1 promoter
  • Rationally designed, asymmetric hairpins optimized for anti-sense microRNA production
  • Efficient suppression of specific endogenous microRNAs
  • Reliable delivery to dividing or non-dividing cells
  • Selection/sorting for transfected/transduced cells with either copGFP or puromycin

Conduct high-throughput phenotypic screens with our pool of pre-packaged lentiviral particles containing our entire miRZip collection (Cat.# MZIPPLVA-1).

How It Works

Screening with the miRZip Library is straightforward

Screening with the miRZip Library is straightforward

  • Transduce the miRZip Library into target cells
  • Treat to induce phenotype
  • Use a functional assay (invasion assay, differentiation assay, proliferation assay, apoptosis assay, etc.) to select for cells exhibiting the desired phenotype
  • Identify miR effectors by amplifying effector miR and sequencing

Supporting Data

See SBI’s mirZip technology in action

miRZip constructs express high levels of anti-microRNAs

miRZip constructs express high levels of anti-microRNAs

Figure 1. miRZip constructs express high levels of anti-microRNAs. miRZip constructs were transfected into HEK293 cells, total RNA isolated, and miRZip levels measured using SBI’s QuantiMir Assay (Cat.# RA420A-1). Compared to QuantiMir assays performed on RNA isolated from non-transfected cells, the miRZip-transfected cells delivered high expression levels of the specific miRZip anti-miRNAs.

Efficient modulation of target protein levels by SBI’s Lenti-miR and miRZip technologies Target protein levels are efficiently modulated by SBI

Figure 2. Target protein levels are efficiently modulated by SBI’s Lenti-miR-29a and miRZip-29a microRNA constructs.

miRZip technology is effective in a functional assaymiRZip-21 and miRZip-145 who anti-oncogenic and oncogenic activity, respectively, in a cell invasion assay using MDA-MB-231 breast cancer cellsmiRZip-145 lentivirus inhibited endogenous miR-145 and elevated protein expression levels of the miR-145 target oncogene c-Myc

Figure 3. miRZip-21 and miRZip-145 exhibit anti-oncogenic and oncogenic activity, respectively, in a cell invasion assay using MDA-MB-231 breast cancer cells. (Top panel) miR-21 is an oncogenic miRNA and miR-145 is a tumor suppressing miRNA. Transduction of miRZip-21 (the anti-miR21 lentiviral construct) and miRZip-145 (the anti-miR145 lentiviral construct), inhibited the respective miRs, resulting in a lower percentage of invading cells in miRZip-21-transduced cells and a higher percentage of invading cells in miRZip-145-transduced cells. (Bottom panel) Additionally, the miRZip-145 lentivirus inhibited endogenous miR-145 and elevated protein expression levels of the miR-145 target oncogene c-Myc.


Citations

  • Liu, A, et al. (2017) Antagonizing miR-455-3p inhibits chemoresistance and aggressiveness in esophageal squamous cell carcinoma. Mol. Cancer. 2017 Jun 21; 16(1):106. PM ID: 28633632
  • Wu, Q, et al. (2017) Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats. Molecular Medicine Reports. 2017 Jun 8;:1578-1583. Link: Molecular Medicine Reports
  • Sun, J, et al. (2017) Mir-367 is downregulated in coronary artery disease and its overexpression exerts anti-inflammatory effect via inhibition of the NF-κB-activated inflammatory pathway. International Journal of Clinical and Experimental Pathology. 2017 Apr 15; 10(4):4047-4057. Link: International Journal of Clinical and Experimental Pathology
  • Guo, GC, et al. (2017) microRNA-761 induces aggressive phenotypes in triple-negative breast cancer cells by repressing TRIM29 expression. Cell Oncol (Dordr). 2017 Apr 1; 40(2):157-166. PM ID: 28054302
  • Yang, TB, et al. (2017) Mutual reinforcement between telomere capping and canonical Wnt signalling in the intestinal stem cell niche. Nat Commun. 2017 Mar 17; 8:14766. PM ID: 28303901
  • Su, K, et al. (2017) miR-940 upregulation contributes to human cervical cancer progression through p27 and PTEN inhibition.. Int. J. Oncol.. 2017 Mar 7;. PM ID: 28350106
  • Moyal, L, et al. (2017) Oncogenic role of microRNA-155 in mycosis fungoides: an in vitro and xenograft mouse model study. Br. J. Dermatol.. 2017 Mar 3;. PM ID: 28256712
  • Li, G, et al. (2017) The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis. Oncogene. 2017 Feb 16; 36(7):989-998. PM ID: 27641336
  • Teteloshvili, N, et al. (2017) Argonaute 2 immunoprecipitation revealed large tumor suppressor kinase 1 as a novel proapoptotic target of miR-21 in T cells. FEBS J.. 2017 Feb 1; 284(4):555-567. PM ID: 28075055
  • Spitschak, A, et al. (2017) MiR-182 promotes cancer invasion by linking RET oncogene activated NF-κB to loss of the HES1/Notch1 regulatory circuit. Mol. Cancer. 2017 Jan 26; 16(1):24. PM ID: 28122586
  • Beezhold, K, Klei, LR & Barchowsky, A. (2017) Regulation of cyclin D1 by arsenic and microRNA inhibits adipogenesis. Toxicol. Lett.. 2017 Jan 4; 265:147-155. PM ID: 27932253
  • Poon, VY, et al. (2016) miR-27b shapes the presynaptic transcriptome and influences neurotransmission by silencing the polycomb group protein Bmi1. BMC Genomics. 2016 Oct 4; 17(1):777. PM ID: 27716060
  • Shen, X, et al. (2016) miR-322/-503 cluster is expressed in the earliest cardiac progenitor cells and drives cardiomyocyte specification. Proc. Natl. Acad. Sci. U.S.A.. 2016 Aug 23; 113(34):9551-6. PM ID: 27512039
  • Langsch, S, et al. (2016) miR-29b Mediates NF-κB Signaling in KRAS-Induced Non-Small Cell Lung Cancers.. Cancer Res.. 2016 Jul 15; 76(14):4160-9. PM ID: 27199349
  • Wang, S, et al. (2016) MicroRNA-mediated epigenetic targeting of Survivin significantly enhances the antitumor activity of paclitaxel against non-small cell lung cancer. Oncotarget. 2016 Jun 21; 7(25):37693-37713. PM ID: 27177222
  • Zheng, D, et al. (2016) Inhibition of MicroRNA 195 Prevents Apoptosis and Multiple-Organ Injury in Mouse Models of Sepsis. J. Infect. Dis.. 2016 May 15; 213(10):1661-70. PM ID: 26704614
  • Tan, Z, et al. (2016) MicroRNA-1229 overexpression promotes cell proliferation and tumorigenicity and activates Wnt/β-catenin signaling in breast cancer. Oncotarget. 2016 Apr 26; 7(17):24076-87. PM ID: 26992223
  • Lin, SC, et al. (2016) Dysregulation of miRNAs-COUP-TFII-FOXM1-CENPF axis contributes to the metastasis of prostate cancer. Nat Commun. 2016 Apr 25; 7:11418. PM ID: 27108958
  • Hong, X, et al. (2016) MiR-448 promotes glycolytic metabolism of gastric cancer by downregulating KDM2B. Oncotarget. 2016 Apr 19; 7(16):22092-102. PM ID: 26989077
  • Liang, ML, et al. (2016) Downregulation of miR-137 and miR-6500-3p promotes cell proliferation in pediatric high-grade gliomas. Oncotarget. 2016 Apr 12; 7(15):19723-37. PM ID: 26933822