pPACKH1 HIV Lentivector Packaging Kit

Generate high titers of transduction-ready pseudoviral particles with this HIV-based Lentivector Packaging Kit
  • Effectively leverage the powerful lentiviral transduction platform with SBI’s optimized HIV-based and FIV-based packaging systems
  • Easily generate high-titer lentiviral preparations
  • Efficiently deliver most third-generation lentivector expression constructs
  • Successfully reach:
    • A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles
    • A broad range of cell types including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more

Products

Catalog Number Description Size Price Quantity Add to Cart
LV500A-1 pPACKH1 HIV Lentivector Packaging Kit 10 Transfections $519
- +
LV510A-1 pPACKH1-XL HIV Lentivector Packaging Kit 25 Transfections $1144
- +

Overview

Overview

Produce high-titer lentivirus with this HIV-based Lentivector Packaging Kit

Designed to efficiently package most third-generation lentivectors, the pPACKH1 HIV Lentivector Packaging Kit makes it easy to deliver and express lentiviral constructs in a wide-range of mammalian cells.

  • Effectively leverage the powerful lentiviral transduction platform with SBI’s optimized HIV-based and FIV-based packaging systems
  • Easily generate high-titer lentiviral preparations
  • Efficiently deliver most third-generation lentivector expression constructs
  • Successfully reach:
    • A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles
    • A broad range of cell types including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more

The pPACKH1 Packaging Plasmid Mix consists of three plasmids that produce all the structural and replication proteins needed to transcribe and package an RNA copy of the expression lentivector into recombinant, VSV-G-pseudotyped lentiviral particles. Just co-transfect the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells, isolate virus particles, and transduce into target cells. Upon transduction, the lentivector construct becomes stably integrated into the genome of the target cells for long-term expression of genes, miRNAs, shRNAs, etc.

How It Works

How It Works

The lentivirus packaging, production, and transduction workflow

Use the pPACKH1 HIV Lentivector Packaging Kit to package your lentivector expression construct into high-titer lentivirus particle preparations

Supporting Data

Supporting Data

SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism

SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism

Resources

Citations

  • Zhang, T, et al. (2022) MicroRNA-377-3p inhibits hepatocellular carcinoma growth and metastasis through negative regulation of CPT1C-mediated fatty acid oxidation. Cancer & metabolism. 1970 Jan 1; 10(1):2. PM ID: 35057851
  • Biswas, S, et al. (2022) Modulation of HIV Replication in Monocyte-Derived Macrophages (MDM) by Host Antiviral Factors Secretory Leukocyte Protease Inhibitor and Serpin Family C Member 1 Induced by Steroid Hormones. Viruses. 1970 Jan 1; 14(1). PM ID: 35062299
  • Xiao, Y, et al. (2022) A targeted extracellular vesicles loaded with montelukast in the treatment of demyelinating diseases. Biochemical and biophysical research communications. 1970 Jan 1; 594:31-37. PM ID: 35066377
  • Shimizu, Y, et al. (2022) Development of a novel Indium-111 radiolabeled mogamulizumab targeting CCR4 for imaging adult T-cell leukemia/lymphoma in vivo. Annals of nuclear medicine. 1970 Jan 1;. PM ID: 35034259
  • Shi, C, et al. (2022) MicroRNA‐144‐3p controls the apoptosis of pulmonary artery endothelial cells in pulmonary arterial hypertension via the BMPR2/Smad4 signaling pathway. Clinical and Translational Discovery. 1970 Jan 1; 2(1). Link: Clinical and Translational Discovery
  • Mulcahy, EQX, et al. (2022) MicroRNA 3928 Suppresses Glioblastoma through Downregulation of Several Oncogenes and Upregulation of p53. International journal of molecular sciences. 1970 Jan 1; 23(7). PM ID: 35409289
  • Morris, A, et al. (2022) Exploration of naphthoquinone analogs in targeting the TCF-DNA interaction to inhibit the Wnt/β-catenin signaling pathway. Bioorganic chemistry. 1970 Jan 1; 124:105812. PM ID: 35447408
  • Wang, Q, et al. (2022) The Mechanism Underlying the Regulation of LncRNA-ASLNC18810 Involved in the Abnormal Function of Vascular Endothelial Cell in Atherosclerosis: Its Function as a microRNA (miRNA) Sponge for miR-559. Journal of cardiovascular translational research. 1970 Jan 1;. PM ID: 35377130
  • Zou, R, et al. (2022) Empagliflozin attenuates cardiac microvascular ischemia/reperfusion injury through improving mitochondrial homeostasis. Cardiovascular diabetology. 1970 Jan 1; 21(1):106. PM ID: 35705980
  • Zhao, H, et al. (2021) Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p. Theranostics. 1970 Jan 1; 11(15):7600-7615. PM ID: 34158869
  • He, Q, et al. (2021) Cancer-secreted exosomal miR-21-5p induces angiogenesis and vascular permeability by targeting KRIT1. Cell death & disease. 1970 Jan 1; 12(6):576. PM ID: 34088891
  • Luo, M, et al. (2021) PD0325901, an ERK inhibitor, enhances the efficacy of PD-1 inhibitor in non-small cell lung carcinoma. Acta Pharmaceutica Sinica B. 1970 Jan 1;. Link: Acta Pharmaceutica Sinica B
  • Golubovskaya, V, et al. (2021) Novel CD37, Humanized CD37 and Bi-Specific Humanized CD37-CD19 CAR-T Cells Specifically Target Lymphoma. Cancers. 1970 Jan 1; 13(5). PM ID: 33652767
  • Clark, B. (2021) ANTI-GLUTEN SYNTHETIC NOTCH RECEPTOR T CELLS FOR THE TREATMENT OF CELIAC DISEASE. Thesis. 1970 Jan 1;. Link: Thesis
  • Rong, QX, et al. (2021) GM-CSF mediates immune evasion via upregulation of PD-L1 expression in extranodal natural killer/T cell lymphoma. Molecular cancer. 1970 Jan 1; 20(1):80. PM ID: 34051805
  • Luo, J, et al. (2021) lncRNA GAS6-AS1 inhibits progression and glucose metabolism reprogramming in LUAD via repressing E2F1-mediated transcription of GLUT1. Molecular Therapy - Nucleic Acids. 1970 Jan 1; 25:11-24. Link: Molecular Therapy - Nucleic Acids
  • Sharad, S & Kapur, S. (2021) Indian Herb-Derived Phytoconstituent-Based Antiviral, Antimicrobial and Antifungal Formulation: An Oral Rinse Candidate for Oral Hygiene and the Potential Prevention of COVID-19 Outbreaks. Pathogens (Basel, Switzerland). 1970 Jan 1; 10(9). PM ID: 34578161
  • Zhang, GH, et al. (2021) Tβ4 ameliorates oxidative damage and apoptosis through ERK/MAPK and 5-HT1A signaling pathway in Aβ insulted SH-SY5Y cells. Life sciences. 1970 Jan 1;:120178. PM ID: 34838849
  • Lee, HH, et al. (2021) Therapeutic effiacy of T cells expressing chimeric antigen receptor derived from a mesothelin-specific scFv in orthotopic human pancreatic cancer animal models. Neoplasia (New York, N.Y.). 1970 Jan 1; 24(2):98-108. PM ID: 34954452
  • Slater, BT, et al. (2020) Structural insight into T cell coinhibition by PD-1H (VISTA). Proc. Natl. Acad. Sci. U.S.A.. 1970 Jan 1; 117(3):1648-1657. PM ID: 31919279

Products

Catalog Number Description Size Price Quantity Add to Cart
LV500A-1 pPACKH1 HIV Lentivector Packaging Kit 10 Transfections $519
- +
LV510A-1 pPACKH1-XL HIV Lentivector Packaging Kit 25 Transfections $1144
- +

Overview

Overview

Produce high-titer lentivirus with this HIV-based Lentivector Packaging Kit

Designed to efficiently package most third-generation lentivectors, the pPACKH1 HIV Lentivector Packaging Kit makes it easy to deliver and express lentiviral constructs in a wide-range of mammalian cells.

  • Effectively leverage the powerful lentiviral transduction platform with SBI’s optimized HIV-based and FIV-based packaging systems
  • Easily generate high-titer lentiviral preparations
  • Efficiently deliver most third-generation lentivector expression constructs
  • Successfully reach:
    • A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles
    • A broad range of cell types including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more

The pPACKH1 Packaging Plasmid Mix consists of three plasmids that produce all the structural and replication proteins needed to transcribe and package an RNA copy of the expression lentivector into recombinant, VSV-G-pseudotyped lentiviral particles. Just co-transfect the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells, isolate virus particles, and transduce into target cells. Upon transduction, the lentivector construct becomes stably integrated into the genome of the target cells for long-term expression of genes, miRNAs, shRNAs, etc.

How It Works

How It Works

The lentivirus packaging, production, and transduction workflow

Use the pPACKH1 HIV Lentivector Packaging Kit to package your lentivector expression construct into high-titer lentivirus particle preparations

Supporting Data

Supporting Data

SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism

SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism

Citations

  • Zhang, T, et al. (2022) MicroRNA-377-3p inhibits hepatocellular carcinoma growth and metastasis through negative regulation of CPT1C-mediated fatty acid oxidation. Cancer & metabolism. 1970 Jan 1; 10(1):2. PM ID: 35057851
  • Biswas, S, et al. (2022) Modulation of HIV Replication in Monocyte-Derived Macrophages (MDM) by Host Antiviral Factors Secretory Leukocyte Protease Inhibitor and Serpin Family C Member 1 Induced by Steroid Hormones. Viruses. 1970 Jan 1; 14(1). PM ID: 35062299
  • Xiao, Y, et al. (2022) A targeted extracellular vesicles loaded with montelukast in the treatment of demyelinating diseases. Biochemical and biophysical research communications. 1970 Jan 1; 594:31-37. PM ID: 35066377
  • Shimizu, Y, et al. (2022) Development of a novel Indium-111 radiolabeled mogamulizumab targeting CCR4 for imaging adult T-cell leukemia/lymphoma in vivo. Annals of nuclear medicine. 1970 Jan 1;. PM ID: 35034259
  • Shi, C, et al. (2022) MicroRNA‐144‐3p controls the apoptosis of pulmonary artery endothelial cells in pulmonary arterial hypertension via the BMPR2/Smad4 signaling pathway. Clinical and Translational Discovery. 1970 Jan 1; 2(1). Link: Clinical and Translational Discovery
  • Mulcahy, EQX, et al. (2022) MicroRNA 3928 Suppresses Glioblastoma through Downregulation of Several Oncogenes and Upregulation of p53. International journal of molecular sciences. 1970 Jan 1; 23(7). PM ID: 35409289
  • Morris, A, et al. (2022) Exploration of naphthoquinone analogs in targeting the TCF-DNA interaction to inhibit the Wnt/β-catenin signaling pathway. Bioorganic chemistry. 1970 Jan 1; 124:105812. PM ID: 35447408
  • Wang, Q, et al. (2022) The Mechanism Underlying the Regulation of LncRNA-ASLNC18810 Involved in the Abnormal Function of Vascular Endothelial Cell in Atherosclerosis: Its Function as a microRNA (miRNA) Sponge for miR-559. Journal of cardiovascular translational research. 1970 Jan 1;. PM ID: 35377130
  • Zou, R, et al. (2022) Empagliflozin attenuates cardiac microvascular ischemia/reperfusion injury through improving mitochondrial homeostasis. Cardiovascular diabetology. 1970 Jan 1; 21(1):106. PM ID: 35705980
  • Zhao, H, et al. (2021) Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p. Theranostics. 1970 Jan 1; 11(15):7600-7615. PM ID: 34158869
  • He, Q, et al. (2021) Cancer-secreted exosomal miR-21-5p induces angiogenesis and vascular permeability by targeting KRIT1. Cell death & disease. 1970 Jan 1; 12(6):576. PM ID: 34088891
  • Luo, M, et al. (2021) PD0325901, an ERK inhibitor, enhances the efficacy of PD-1 inhibitor in non-small cell lung carcinoma. Acta Pharmaceutica Sinica B. 1970 Jan 1;. Link: Acta Pharmaceutica Sinica B
  • Golubovskaya, V, et al. (2021) Novel CD37, Humanized CD37 and Bi-Specific Humanized CD37-CD19 CAR-T Cells Specifically Target Lymphoma. Cancers. 1970 Jan 1; 13(5). PM ID: 33652767
  • Clark, B. (2021) ANTI-GLUTEN SYNTHETIC NOTCH RECEPTOR T CELLS FOR THE TREATMENT OF CELIAC DISEASE. Thesis. 1970 Jan 1;. Link: Thesis
  • Rong, QX, et al. (2021) GM-CSF mediates immune evasion via upregulation of PD-L1 expression in extranodal natural killer/T cell lymphoma. Molecular cancer. 1970 Jan 1; 20(1):80. PM ID: 34051805
  • Luo, J, et al. (2021) lncRNA GAS6-AS1 inhibits progression and glucose metabolism reprogramming in LUAD via repressing E2F1-mediated transcription of GLUT1. Molecular Therapy - Nucleic Acids. 1970 Jan 1; 25:11-24. Link: Molecular Therapy - Nucleic Acids
  • Sharad, S & Kapur, S. (2021) Indian Herb-Derived Phytoconstituent-Based Antiviral, Antimicrobial and Antifungal Formulation: An Oral Rinse Candidate for Oral Hygiene and the Potential Prevention of COVID-19 Outbreaks. Pathogens (Basel, Switzerland). 1970 Jan 1; 10(9). PM ID: 34578161
  • Zhang, GH, et al. (2021) Tβ4 ameliorates oxidative damage and apoptosis through ERK/MAPK and 5-HT1A signaling pathway in Aβ insulted SH-SY5Y cells. Life sciences. 1970 Jan 1;:120178. PM ID: 34838849
  • Lee, HH, et al. (2021) Therapeutic effiacy of T cells expressing chimeric antigen receptor derived from a mesothelin-specific scFv in orthotopic human pancreatic cancer animal models. Neoplasia (New York, N.Y.). 1970 Jan 1; 24(2):98-108. PM ID: 34954452
  • Slater, BT, et al. (2020) Structural insight into T cell coinhibition by PD-1H (VISTA). Proc. Natl. Acad. Sci. U.S.A.. 1970 Jan 1; 117(3):1648-1657. PM ID: 31919279