pPACKH1 HIV Lentivector Packaging Kit
Generate high titers of transduction-ready pseudoviral particles with this HIV-based Lentivector Packaging Kit
- Effectively leverage the powerful lentiviral transduction platform with SBI’s optimized HIV-based and FIV-based packaging systems
- Easily generate high-titer lentiviral preparations
- Efficiently deliver most third-generation lentivector expression constructs
-
Successfully reach:
- A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles
- A broad range of cell types including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| LV500A-1 | pPACKH1 HIV Lentivector Packaging Kit | 10 Transfections | $529 |
|
||||
| LV510A-1 | pPACKH1-XL HIV Lentivector Packaging Kit | 25 Transfections | $1167 |
|
||||
Overview
Overview
Produce high-titer lentivirus with this HIV-based Lentivector Packaging Kit
Designed to efficiently package most third-generation lentivectors, the pPACKH1 HIV Lentivector Packaging Kit makes it easy to deliver and express lentiviral constructs in a wide-range of mammalian cells.
- Effectively leverage the powerful lentiviral transduction platform with SBI’s optimized HIV-based and FIV-based packaging systems
- Easily generate high-titer lentiviral preparations
- Efficiently deliver most third-generation lentivector expression constructs
- Successfully reach:
- A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles
- A broad range of cell types including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more
The pPACKH1 Packaging Plasmid Mix consists of three plasmids that produce all the structural and replication proteins needed to transcribe and package an RNA copy of the expression lentivector into recombinant, VSV-G-pseudotyped lentiviral particles. Just co-transfect the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells, isolate virus particles, and transduce into target cells. Upon transduction, the lentivector construct becomes stably integrated into the genome of the target cells for long-term expression of genes, miRNAs, shRNAs, etc.
How It Works
How It Works
The lentivirus packaging, production, and transduction workflow
Supporting Data
Supporting Data
SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism
FAQs
The pPACKH1 HIV Lentivector Packaging Kit is designed to efficiently package most third-generation lentivectors, allowing for the delivery and expression of lentiviral constructs in a wide range of mammalian cells.
The pPACKH1 HIV Lentivector Packaging Kit can transduce a broad range of cell types, including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more.
The pPACKH1 Packaging Plasmid Mix contains three plasmids that produce all the structural and replication proteins needed to transcribe and package an RNA copy of the expression lentivector into recombinant, VSV-G-pseudo typed lentiviral particles. Co-transfect the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells, isolate virus particles, and transduce into target cells. Upon transduction, the lentivector construct becomes stably integrated into the genome of the target cells for long-term expression of genes, miRNAs, shRNAs, etc.
The pPACKH1 HIV Lentivector Packaging Kit enables you to easily generate high-titer lentiviral preparations by co-transfecting the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells.
The pPACKH1 HIV Lentivector Packaging Kit is based on an HIV-derived system, while the pPACKF1 FIV (Cat. # LV100A-1 and LV100A-5) Lentivector Packaging Kit uses an FIV-derived system. Both systems are designed to effectively leverage the powerful lentiviral transduction platform for gene delivery and expression in a wide range of mammalian cells.
To speak to a specialist, you can either contact System Biosciences via our website, e-mail us at info@systembio.com, or call our toll-free number at 1-888-266-5066.
Yes, the pPACKH1 HIV Lentivector Packaging Kit is available in two sizes: LV500A-1 with 10 transfections and LV510A-1 with 25 transfections. Price is subject to change from year to year.
Resources
Related Products
Citations
-
Mo, Y, et al. (2023) Tumor-secreted exosomal miR-141 activates tumor-stroma interactions and controls premetastatic niche formation in ovarian cancer metastasis. Molecular cancer. 2023; 22(1):4. PM ID: 36624516
-
Zhou, Q, et al. (2023) Catechol-O-Methyltransferase Loss Drives Cell-Specific Nociceptive Signaling via the Enteric COMT/miR-155/TNF-α Axis. Gastroenterology. 2023;. PM ID: 36623778
-
Ko, JY, et al. (2023) Micro Ribonucleic Acid-29a (miR-29a) Antagonist Normalizes Bone Metabolism in Osteogenesis Imperfecta (OI) Mice Model. Biomedicines. 2023; 11(2). PM ID: 36831000
-
Li, J, et al. (2023) Secreted proteins MDK, WFDC2, and CXCL14 as candidate biomarkers for early diagnosis of lung adenocarcinoma. BMC cancer. 2023; 23(1):110. PM ID: 36721112
-
Guan, Y, et al. (2023) Ginsenoside Rg1 attenuates cerebral ischemia-reperfusion injury through inhibiting the inflammatory activation of microglia. Experimental cell research. 2023; 426(1):113552. PM ID: 36914061
-
Cui, H, et al. (2023) Effect and Mechanism of lncRNA-PCMF1/hsa-miR-137/Twist1 Axis Involved in the EMT Regulation of Prostate Cancer Cells. Molecular biotechnology. 2023;. PM ID: 36906874
-
Krohl, P, et al. (2023) Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach. Cell Reports Methods. 2023; 3(3):100429. Link: Cell Reports Methods
-
Zhang, T, et al. (2022) MicroRNA-377-3p inhibits hepatocellular carcinoma growth and metastasis through negative regulation of CPT1C-mediated fatty acid oxidation. Cancer & metabolism. 2022; 10(1):2. PM ID: 35057851
-
Biswas, S, et al. (2022) Modulation of HIV Replication in Monocyte-Derived Macrophages (MDM) by Host Antiviral Factors Secretory Leukocyte Protease Inhibitor and Serpin Family C Member 1 Induced by Steroid Hormones. Viruses. 2022; 14(1). PM ID: 35062299
-
Xiao, Y, et al. (2022) A targeted extracellular vesicles loaded with montelukast in the treatment of demyelinating diseases. Biochemical and biophysical research communications. 2022; 594:31-37. PM ID: 35066377
-
Shimizu, Y, et al. (2022) Development of a novel Indium-111 radiolabeled mogamulizumab targeting CCR4 for imaging adult T-cell leukemia/lymphoma in vivo. Annals of nuclear medicine. 2022;. PM ID: 35034259
-
Shi, C, et al. (2022) MicroRNA‐144‐3p controls the apoptosis of pulmonary artery endothelial cells in pulmonary arterial hypertension via the BMPR2/Smad4 signaling pathway. Clinical and Translational Discovery. 2022; 2(1). Link: Clinical and Translational Discovery
-
Mulcahy, EQX, et al. (2022) MicroRNA 3928 Suppresses Glioblastoma through Downregulation of Several Oncogenes and Upregulation of p53. International journal of molecular sciences. 2022; 23(7). PM ID: 35409289
-
Morris, A, et al. (2022) Exploration of naphthoquinone analogs in targeting the TCF-DNA interaction to inhibit the Wnt/β-catenin signaling pathway. Bioorganic chemistry. 2022; 124:105812. PM ID: 35447408
-
Wang, Q, et al. (2022) The Mechanism Underlying the Regulation of LncRNA-ASLNC18810 Involved in the Abnormal Function of Vascular Endothelial Cell in Atherosclerosis: Its Function as a microRNA (miRNA) Sponge for miR-559. Journal of cardiovascular translational research. 2022;. PM ID: 35377130
-
Zou, R, et al. (2022) Empagliflozin attenuates cardiac microvascular ischemia/reperfusion injury through improving mitochondrial homeostasis. Cardiovascular diabetology. 2022; 21(1):106. PM ID: 35705980
-
Noguchi, S, et al. (2022) 13C metabolic flux analysis clarifies distinct metabolic phenotypes of cancer cell spheroid mimicking tumor hypoxia. Metabolic Engineering. 2022;. Link: Metabolic Engineering
-
Sun, Z, et al. (2022) Exosomal lncRNA TUG1 derived from human urine-derived stem cells attenuates renal ischemia/reperfusion injury by interacting with SRSF1 to regulate ASCL4-mediated ferroptosis. Stem cell research & therapy. 2022; 13(1):297. PM ID: 35841017
-
Cui, R, et al. (2022) PARD3 gene variation as candidate cause of nonsyndromic cleft palate only. Journal of cellular and molecular medicine. 2022; 26(15):4292-4304. PM ID: 35789100
-
Yang, H, et al. (2022) Engineered bispecific antibodies targeting the interleukin-6 and -8 receptors potently inhibit cancer cell migration and tumor metastasis. Molecular therapy : the journal of the American Society of Gene Therapy. 2022;. PM ID: 35841152
- See More
pPACKH1 HIV Lentivector Packaging Kit
$529.00
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| LV500A-1 | pPACKH1 HIV Lentivector Packaging Kit | 10 Transfections | $529 |
|
||||
| LV510A-1 | pPACKH1-XL HIV Lentivector Packaging Kit | 25 Transfections | $1167 |
|
||||
Overview
Overview
Produce high-titer lentivirus with this HIV-based Lentivector Packaging Kit
Designed to efficiently package most third-generation lentivectors, the pPACKH1 HIV Lentivector Packaging Kit makes it easy to deliver and express lentiviral constructs in a wide-range of mammalian cells.
- Effectively leverage the powerful lentiviral transduction platform with SBI’s optimized HIV-based and FIV-based packaging systems
- Easily generate high-titer lentiviral preparations
- Efficiently deliver most third-generation lentivector expression constructs
- Successfully reach:
- A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles
- A broad range of cell types including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more
The pPACKH1 Packaging Plasmid Mix consists of three plasmids that produce all the structural and replication proteins needed to transcribe and package an RNA copy of the expression lentivector into recombinant, VSV-G-pseudotyped lentiviral particles. Just co-transfect the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells, isolate virus particles, and transduce into target cells. Upon transduction, the lentivector construct becomes stably integrated into the genome of the target cells for long-term expression of genes, miRNAs, shRNAs, etc.
How It Works
How It Works
The lentivirus packaging, production, and transduction workflow
Supporting Data
Supporting Data
SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism
FAQs
The pPACKH1 HIV Lentivector Packaging Kit is designed to efficiently package most third-generation lentivectors, allowing for the delivery and expression of lentiviral constructs in a wide range of mammalian cells.
The pPACKH1 HIV Lentivector Packaging Kit can transduce a broad range of cell types, including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more.
The pPACKH1 Packaging Plasmid Mix contains three plasmids that produce all the structural and replication proteins needed to transcribe and package an RNA copy of the expression lentivector into recombinant, VSV-G-pseudo typed lentiviral particles. Co-transfect the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells, isolate virus particles, and transduce into target cells. Upon transduction, the lentivector construct becomes stably integrated into the genome of the target cells for long-term expression of genes, miRNAs, shRNAs, etc.
The pPACKH1 HIV Lentivector Packaging Kit enables you to easily generate high-titer lentiviral preparations by co-transfecting the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells.
The pPACKH1 HIV Lentivector Packaging Kit is based on an HIV-derived system, while the pPACKF1 FIV (Cat. # LV100A-1 and LV100A-5) Lentivector Packaging Kit uses an FIV-derived system. Both systems are designed to effectively leverage the powerful lentiviral transduction platform for gene delivery and expression in a wide range of mammalian cells.
To speak to a specialist, you can either contact System Biosciences via our website, e-mail us at info@systembio.com, or call our toll-free number at 1-888-266-5066.
Yes, the pPACKH1 HIV Lentivector Packaging Kit is available in two sizes: LV500A-1 with 10 transfections and LV510A-1 with 25 transfections. Price is subject to change from year to year.
Citations
-
Mo, Y, et al. (2023) Tumor-secreted exosomal miR-141 activates tumor-stroma interactions and controls premetastatic niche formation in ovarian cancer metastasis. Molecular cancer. 2023; 22(1):4. PM ID: 36624516
-
Zhou, Q, et al. (2023) Catechol-O-Methyltransferase Loss Drives Cell-Specific Nociceptive Signaling via the Enteric COMT/miR-155/TNF-α Axis. Gastroenterology. 2023;. PM ID: 36623778
-
Ko, JY, et al. (2023) Micro Ribonucleic Acid-29a (miR-29a) Antagonist Normalizes Bone Metabolism in Osteogenesis Imperfecta (OI) Mice Model. Biomedicines. 2023; 11(2). PM ID: 36831000
-
Li, J, et al. (2023) Secreted proteins MDK, WFDC2, and CXCL14 as candidate biomarkers for early diagnosis of lung adenocarcinoma. BMC cancer. 2023; 23(1):110. PM ID: 36721112
-
Guan, Y, et al. (2023) Ginsenoside Rg1 attenuates cerebral ischemia-reperfusion injury through inhibiting the inflammatory activation of microglia. Experimental cell research. 2023; 426(1):113552. PM ID: 36914061
-
Cui, H, et al. (2023) Effect and Mechanism of lncRNA-PCMF1/hsa-miR-137/Twist1 Axis Involved in the EMT Regulation of Prostate Cancer Cells. Molecular biotechnology. 2023;. PM ID: 36906874
-
Krohl, P, et al. (2023) Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach. Cell Reports Methods. 2023; 3(3):100429. Link: Cell Reports Methods
-
Zhang, T, et al. (2022) MicroRNA-377-3p inhibits hepatocellular carcinoma growth and metastasis through negative regulation of CPT1C-mediated fatty acid oxidation. Cancer & metabolism. 2022; 10(1):2. PM ID: 35057851
-
Biswas, S, et al. (2022) Modulation of HIV Replication in Monocyte-Derived Macrophages (MDM) by Host Antiviral Factors Secretory Leukocyte Protease Inhibitor and Serpin Family C Member 1 Induced by Steroid Hormones. Viruses. 2022; 14(1). PM ID: 35062299
-
Xiao, Y, et al. (2022) A targeted extracellular vesicles loaded with montelukast in the treatment of demyelinating diseases. Biochemical and biophysical research communications. 2022; 594:31-37. PM ID: 35066377
-
Shimizu, Y, et al. (2022) Development of a novel Indium-111 radiolabeled mogamulizumab targeting CCR4 for imaging adult T-cell leukemia/lymphoma in vivo. Annals of nuclear medicine. 2022;. PM ID: 35034259
-
Shi, C, et al. (2022) MicroRNA‐144‐3p controls the apoptosis of pulmonary artery endothelial cells in pulmonary arterial hypertension via the BMPR2/Smad4 signaling pathway. Clinical and Translational Discovery. 2022; 2(1). Link: Clinical and Translational Discovery
-
Mulcahy, EQX, et al. (2022) MicroRNA 3928 Suppresses Glioblastoma through Downregulation of Several Oncogenes and Upregulation of p53. International journal of molecular sciences. 2022; 23(7). PM ID: 35409289
-
Morris, A, et al. (2022) Exploration of naphthoquinone analogs in targeting the TCF-DNA interaction to inhibit the Wnt/β-catenin signaling pathway. Bioorganic chemistry. 2022; 124:105812. PM ID: 35447408
-
Wang, Q, et al. (2022) The Mechanism Underlying the Regulation of LncRNA-ASLNC18810 Involved in the Abnormal Function of Vascular Endothelial Cell in Atherosclerosis: Its Function as a microRNA (miRNA) Sponge for miR-559. Journal of cardiovascular translational research. 2022;. PM ID: 35377130
-
Zou, R, et al. (2022) Empagliflozin attenuates cardiac microvascular ischemia/reperfusion injury through improving mitochondrial homeostasis. Cardiovascular diabetology. 2022; 21(1):106. PM ID: 35705980
-
Noguchi, S, et al. (2022) 13C metabolic flux analysis clarifies distinct metabolic phenotypes of cancer cell spheroid mimicking tumor hypoxia. Metabolic Engineering. 2022;. Link: Metabolic Engineering
-
Sun, Z, et al. (2022) Exosomal lncRNA TUG1 derived from human urine-derived stem cells attenuates renal ischemia/reperfusion injury by interacting with SRSF1 to regulate ASCL4-mediated ferroptosis. Stem cell research & therapy. 2022; 13(1):297. PM ID: 35841017
-
Cui, R, et al. (2022) PARD3 gene variation as candidate cause of nonsyndromic cleft palate only. Journal of cellular and molecular medicine. 2022; 26(15):4292-4304. PM ID: 35789100
-
Yang, H, et al. (2022) Engineered bispecific antibodies targeting the interleukin-6 and -8 receptors potently inhibit cancer cell migration and tumor metastasis. Molecular therapy : the journal of the American Society of Gene Therapy. 2022;. PM ID: 35841152
- See More