pGreenFire1-LXRE-in-LXRα Lentivector

Study cholesterol homeostasis by co-expressing dscGFP and luciferase in response to Liver X Receptor (LXR) activity at the LXRα promoter
  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

Products

Catalog Number Description Size Price Quantity Add to Cart
TR102A-P pGreenFire1-LXRE in LXR alpha (HepG2 cell line) 2 x 10^6 Cells $3258
- +
TR102PA-P pGreenFire1-LXRE in LXR alpha (plasmid) 10 µg $747
- +
TR102VA-P pGreenFire1-LXRE in LXR alpha (virus) >2 x 10^6 IFUs $747
- +

Overview

Overview

Monitor cholesterol homeostasis signal transduction in real time

With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGreenFire1-LXRE-in-LXRα Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from the Liver X Receptor (LXR) response elements (LXREs) and neighboring regions in the LXRα promoter paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of the LXRE/LXRα transcriptional elements, drives expression of dscGFP and luciferase in response to LXR activity. The result is the ability to quantitatively measure LXR activity at the LXRα promoter by fluorescence and luciferase activity.

  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGreenFire1-LXRE-in-LXRα Lentivector

The pGreenFire1-LXRE-in-LXRα Lentivector is available as a lentivector, pre-packaged virus, and a stable cell line created from the pGreenFire1-LXRE-in-LXRα transduced into HepG2 cells.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

FAQs

Resources

Citations

  • Ishino, T, et al. (2023) Somatic mutations can induce a noninflamed tumour microenvironment via their original gene functions, despite deriving neoantigens. British journal of cancer. 2023;. PM ID: 36732592
  • Pandi, K, et al. (2023) Porphyromonas gingivalis induction of TLR2 association with Vinculin enables PI3K activation and immune evasion. PLoS pathogens. 2023; 19(4):e1011284. PM ID: 37023213
  • Ramachandran, M, et al. (2023) Tailoring vascular phenotype through AAV therapy promotes anti-tumor immunity in glioma. Cancer cell. 2023;. PM ID: 37172581
  • Wen, YC, et al. (2023) CHRM4/AKT/MYCN upregulates interferon alpha-17 in the tumor microenvironment to promote neuroendocrine differentiation of prostate cancer. Cell death & disease. 2023; 14(5):304. PM ID: 37142586
  • Li, X, et al. (2023) Rosmarinic acid ameliorates autoimmune responses through suppression of intracellular nucleic acid-mediated type I interferon expression. Biochemical and Biophysical Research Communications. 2023;. Link: Biochemical and Biophysical Research Communications
  • Ibrahim, L, et al. (2023) Succinylation of a KEAP1 sensor lysine promotes NRF2 activation. bioRxiv : the preprint server for biology. 2023;. PM ID: 37215033
  • Park, CS, et al. (2023) Stromal-induced epithelial-mesenchymal transition induces targetable drug resistance in acute lymphoblastic leukemia. Cell reports. 2023; 42(7):112804. PM ID: 37453060
  • Ouyang, W, et al. (2023) Development of a New Cell-Based AP-1 Gene Reporter Potency Assay for Anti-Anthrax Toxin Therapeutics. Toxins. 2023; 15(9):528. Link: Toxins
  • Zhao, G, et al. (2023) Base editing of the mutated TERT promoter inhibits liver tumor growth. Hepatology (Baltimore, Md.). 2023;. PM ID: 38016019
  • You, S & Bollong, MJ. (2023) A high throughput screen for pharmacological inhibitors of the carbohydrate response element. Scientific data. 2023; 10(1):676. PM ID: 37794069
  • Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 2022;. PM ID: 35483375
  • Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 2022;. Link: slas-discovery.org
  • Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 2022; 21(6):710-720. PM ID: 35606429
  • Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 2022;. Link: Research Square
  • Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 2022; 11(7). PM ID: 35884187
  • Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 2022; 25:101508. PM ID: 35985204
  • Chen, C, et al. (2022) ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Nature communications. 2022; 13(1):6108. PM ID: 36245009
  • Takase, S, et al. (2022) 17β-neriifolin from unripe fruits of Cerbera manghas suppressed cell proliferation via the inhibition of HOXA9-dependent transcription and the induction of apoptosis in the human AML cell line THP-1. Journal of natural medicines. 2022;. PM ID: 36266527
  • Donohue, L, et al. (2022) A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation. Cell Genomics. 2022;:100191. Link: Cell Genomics
  • Caligiuri, SPB, et al. (2022) Hedgehog-interacting protein acts in the habenula to regulate nicotine intake. Proceedings of the National Academy of Sciences of the United States of America. 2022; 119(46):e2209870119. PM ID: 36346845

Products

Catalog Number Description Size Price Quantity Add to Cart
TR102A-P pGreenFire1-LXRE in LXR alpha (HepG2 cell line) 2 x 10^6 Cells $3258
- +
TR102PA-P pGreenFire1-LXRE in LXR alpha (plasmid) 10 µg $747
- +
TR102VA-P pGreenFire1-LXRE in LXR alpha (virus) >2 x 10^6 IFUs $747
- +

Overview

Overview

Monitor cholesterol homeostasis signal transduction in real time

With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGreenFire1-LXRE-in-LXRα Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from the Liver X Receptor (LXR) response elements (LXREs) and neighboring regions in the LXRα promoter paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of the LXRE/LXRα transcriptional elements, drives expression of dscGFP and luciferase in response to LXR activity. The result is the ability to quantitatively measure LXR activity at the LXRα promoter by fluorescence and luciferase activity.

  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGreenFire1-LXRE-in-LXRα Lentivector

The pGreenFire1-LXRE-in-LXRα Lentivector is available as a lentivector, pre-packaged virus, and a stable cell line created from the pGreenFire1-LXRE-in-LXRα transduced into HepG2 cells.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

FAQs

Citations

  • Ishino, T, et al. (2023) Somatic mutations can induce a noninflamed tumour microenvironment via their original gene functions, despite deriving neoantigens. British journal of cancer. 2023;. PM ID: 36732592
  • Pandi, K, et al. (2023) Porphyromonas gingivalis induction of TLR2 association with Vinculin enables PI3K activation and immune evasion. PLoS pathogens. 2023; 19(4):e1011284. PM ID: 37023213
  • Ramachandran, M, et al. (2023) Tailoring vascular phenotype through AAV therapy promotes anti-tumor immunity in glioma. Cancer cell. 2023;. PM ID: 37172581
  • Wen, YC, et al. (2023) CHRM4/AKT/MYCN upregulates interferon alpha-17 in the tumor microenvironment to promote neuroendocrine differentiation of prostate cancer. Cell death & disease. 2023; 14(5):304. PM ID: 37142586
  • Li, X, et al. (2023) Rosmarinic acid ameliorates autoimmune responses through suppression of intracellular nucleic acid-mediated type I interferon expression. Biochemical and Biophysical Research Communications. 2023;. Link: Biochemical and Biophysical Research Communications
  • Ibrahim, L, et al. (2023) Succinylation of a KEAP1 sensor lysine promotes NRF2 activation. bioRxiv : the preprint server for biology. 2023;. PM ID: 37215033
  • Park, CS, et al. (2023) Stromal-induced epithelial-mesenchymal transition induces targetable drug resistance in acute lymphoblastic leukemia. Cell reports. 2023; 42(7):112804. PM ID: 37453060
  • Ouyang, W, et al. (2023) Development of a New Cell-Based AP-1 Gene Reporter Potency Assay for Anti-Anthrax Toxin Therapeutics. Toxins. 2023; 15(9):528. Link: Toxins
  • Zhao, G, et al. (2023) Base editing of the mutated TERT promoter inhibits liver tumor growth. Hepatology (Baltimore, Md.). 2023;. PM ID: 38016019
  • You, S & Bollong, MJ. (2023) A high throughput screen for pharmacological inhibitors of the carbohydrate response element. Scientific data. 2023; 10(1):676. PM ID: 37794069
  • Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 2022;. PM ID: 35483375
  • Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 2022;. Link: slas-discovery.org
  • Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 2022; 21(6):710-720. PM ID: 35606429
  • Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 2022;. Link: Research Square
  • Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 2022; 11(7). PM ID: 35884187
  • Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 2022; 25:101508. PM ID: 35985204
  • Chen, C, et al. (2022) ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Nature communications. 2022; 13(1):6108. PM ID: 36245009
  • Takase, S, et al. (2022) 17β-neriifolin from unripe fruits of Cerbera manghas suppressed cell proliferation via the inhibition of HOXA9-dependent transcription and the induction of apoptosis in the human AML cell line THP-1. Journal of natural medicines. 2022;. PM ID: 36266527
  • Donohue, L, et al. (2022) A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation. Cell Genomics. 2022;:100191. Link: Cell Genomics
  • Caligiuri, SPB, et al. (2022) Hedgehog-interacting protein acts in the habenula to regulate nicotine intake. Proceedings of the National Academy of Sciences of the United States of America. 2022; 119(46):e2209870119. PM ID: 36346845