pLL-EF1a-BFP-T2A-Blast Lenti-Labeler™ Lentivector
Plasmid & Pre-packaged Virus

Reliably label cells with BFP driven by a moderate EF1α promoter (and with blasticidin selection) - great for tracking cells, HTS assays, and more

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pLL-EF1a-BFP-T2A-Blast (Lenti-Labeler™ plasmid)

10 µg
LL225PA-1
$ 575

pLL-EF1a-BFP-T2A-Blast (Lenti-Labeler™ virus)

>2 x10^6 IFUs
LL225VA-1
$ 575
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Overview

Reliable cell labeling, delivered

SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-EF1α-BFP-T2A-Blast Lenti-Labeler™ construct expresses BFP from the EF1α promoter, which delivers moderate expression in most cell types (including primary and stem cells), and co-expresses the blasticidin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA (Cat.# LL225PA-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL225VA-1), SBI’s pLL-EF1α-BFP-T2A-Blast Lenti-Labeler™ is designed for reliability, so you can get to valuable insights faster

pLL-EF1α-BFP-T2A-Blast Lenti-Labeler

  • Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
  • Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
  • Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)

While the pLL-EF1α-BFP-T2A-Blast Lenti-Labeler construct is compatible with most second and third generation packaging plasmid mixes, SBI recommends the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titers and infection of target cells.

Find the right Lenti-Labeler construct for your studies

SBI offers a Lenti-Labeler constructs with a range of selection markers, reporter genes, and two different promoters.

Table 1. Available Lenti-Labeler Constructs

Cat. # Construct Promoter Reporter Selection
LL100PA-1/LL100VA-1 pLL-CMV-GFP-T2A-Puro CMV GFP Puromycin
LL105PA-1/LL105VA-1 pLL-CMV-GFP-T2A-Blast

CMV GFP Blasticidin
LL110PA-1/LL110VA-1 pLL-CMV-RFP-T2A-Puro CMV RFP Puromycin
LL115PA-1/LL115VA-1 pLL-CMV-RFP-T2A-Blast CMV RFP Blasticidin
LL120PA-1/LL120VA-1 pLL-CMV-BFP-T2A-Puro CMV BFP Puromycin
LL125PA-1/LL125VA-1 pLL-CMV-BFP-T2A-Blast CMV BFP Blasticidin
LL150PA-1/LL150VA-1 pLL-CMV-Luciferase-T2A-Puro CMV Luciferase Puromycin
LL200PA-1/LL200VA-1 pLL-EF1α-GFP-T2A-Puro EF1α GFP Puromycin
LL205PA-1/LL205VA-1 pLL-EF1α-GFP-T2A-Blast EF1α GFP Blasticidin
LL210PA-1/LL210VA-1 pLL-EF1α-RFP-T2A-Puro EF1α RFP Puromycin
LL215PA-1/LL215VA-1 pLL-EF1α-RFP-T2A-Blast EF1α RFP Blasticidin
LL220PA-1/LL220VA-1 pLL-EF1α-BFP-T2A-Puro EF1α BFP Puromycin
LL225PA-1/LL225VA-1 pLL-EF1α-BFP-T2A-Blast EF1α BFP Blasticidin
LL250PA-1/LL250VA-1 pLL-EF1α-Luciferase-T2A-Puro EF1α Luciferase Puromycin
LL300PA-1/LL300VA-1 pLL-CMV-rFLuc-T2A-GFP CMV Luciferase & GFP N/A
LL310PA-1/LL310VA-1 pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro CMV Luciferase & GFP Puromycin
LL320PA-1/LL320VA-1 pLL-CMV-rFLuc-T2A-mRFP-mPGK-Puro CMV Luciferase & RFP Puromycin
LL410PA-1/LL410VA-1 pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro EF1α Luciferase & GFP Puromycin
LL420PA-1/LL420VA-1

pLL-EF1a-rFLuc-T2A-mRFP-mPGK-Puro EF1α Luciferase & RFP Puromycin

Supporting Data

SBI’s Lenti-Labelers get your cells labeled efficiently, reliably

Lenti-Labeler

Figure 1. SBI Lenti-Labeler constructs reliably and efficiently label cells. Comparison of the number of fluorescently-labeled cells to the total number of cells seen in the corresponding phase contrast images reveals the high labeling efficiency of SBI’s Lenti-Labeler constructs.


Citations

  • Akerman, AW, et al. (2019) Elevated Wall Tension Leads to Reduced miR-133a in the Thoracic Aorta by Exosome Release. J Am Heart Assoc. 2019 Jan 8; 8(1):e010332. PM ID: 30572760
  • Shao, Y, et al. (2019) Overexpression of CXCR7 promotes mesenchymal stem cells to repair phosgene-induced acute lung injury in rats. Biomedicine & Pharmacotherapy. 2019 Jan 1; 109:1233-1239. Link: Biomedicine & Pharmacotherapy
  • Wang, Z, et al. (2019) Nucleophosmin Phosphorylation as a Diagnostic and Therapeutic Target for Ischemic AKI. J. Am. Soc. Nephrol.. 2019 Jan 1; 30(1):50-62. PM ID: 30573638
  • Xu, L, et al. (2018) MiR-34c ameliorates neuropathic pain by targeting NLRP3 in a mouse model of chronic constriction injury. Neuroscience. 2018 Dec 26;. PM ID: 30593918
  • Sekiba, K, et al. (2018) Pevonedistat, a first-in-class NEDD8-activating enzyme inhibitor, is a potent inhibitor of hepatitis B virus. Hepatology. 2018 Dec 26;. PM ID: 30586159
  • Foerster, F, et al. (2018) Enhanced protection of C57 BL/6 vs Balb/c mice to melanoma liver metastasis is mediated by NK cells. Oncoimmunology. 2018 Dec 26; 7(4):e1409929. PM ID: 29632723
  • Qi, S, et al. (2018) MCPIP1 mediates inflammatory responses induced by lipopolysaccharide and lipoteichoic acid in bovine mammary epithelial cells. Acta Biochim. Biophys. Sin. (Shanghai). 2018 Dec 26;. PM ID: 30590418
  • Hino, N, et al. (2018) An amphipathic helix of vinexin α is necessary for substrate stiffness-dependent conformational change in vinculin. J. Cell. Sci.. 2018 Dec 21;. PM ID: 30578314
  • Wang, J, et al. (2018) Determination of Serum Exosomal H19 as a Noninvasive Biomarker for Bladder Cancer Diagnosis and Prognosis. Med. Sci. Monit.. 2018 Dec 21; 24:9307-9316. PM ID: 30576305
  • Ren, F, et al. (2018) The R132H mutation in IDH1 promotes the recruitment of NK cells through CX3CL1/CX3CR1 chemotaxis and is correlated with a better prognosis in gliomas. Immunol. Cell Biol.. 2018 Dec 21;. PM ID: 30575118
  • Xie, Y, et al. (2018) Molecular network of miR-1343 regulates the pluripotency of porcine pluripotent stem cells via repressing OTX2 expression. RNA Biol. 2018 Dec 20;. PM ID: 30567463
  • Raffo-Romero, A, et al. (2018) Medicinal Leech CNS as a Model for Exosome Studies in the Crosstalk between Microglia and Neurons. Int J Mol Sci. 2018 Dec 19; 19(12). PM ID: 30572617
  • Zhang, J, et al. (2018) Mouse serum protects against total body irradiation-induced hematopoietic system injury by improving the systemic environment after radiation. Free Radic. Biol. Med.. 2018 Dec 19; 131:382-392. PM ID: 30578918
  • Saeed, U, et al. (2018) Parvulin 14 and parvulin 17 bind to HBx and cccDNA and upregulate HBV replication from cccDNA to virion in a HBx-dependent manner. J. Virol.. 2018 Dec 19;. PM ID: 30567987
  • Rostami, M, Haidari, K & Shahbazi, M. (2018) The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells. International Journal of Cell Biology. 2018 Dec 19; 2018:1-14. Link: International Journal of Cell Biology
  • Zeng, Z, et al. (2018) Cancer-derived exosomal miR-25-3p promotes pre-metastatic niche formation by inducing vascular permeability and angiogenesis. Nat Commun. 2018 Dec 19; 9(1):5395. PM ID: 30568162
  • Vasjari, L, et al. (2018) Ras signals principally via Erk in G1 but cooperates with PI3K/Akt for Cyclin D induction and S-phase entry. Cell Cycle. 2018 Dec 18;. PM ID: 30560710
  • Guo, D, et al. (2018) RAB27A promotes melanoma cell invasion and metastasis via regulation of pro-invasive exosomes. Int. J. Cancer. 2018 Dec 17;. PM ID: 30556600
  • Busatto, S, et al. (2018) Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid. Cells. 2018 Dec 16; 7(12). PM ID: 30558352
  • Shenoy, GN, et al. (2018) Sialic Acid-Dependent Inhibition of T Cells by Exosomal Ganglioside GD3 in Ovarian Tumor Microenvironments. J. Immunol.. 2018 Dec 15; 201(12):3750-3758. PM ID: 30446565