An easy-to-produce non-integrating option for gene expression
With virtually no limits on insert size (unlike AAV vectors) Enhanced Episomal Vectors (EEVs) are an excellent choice for non-integrating, non-viral gene expression. Because they replicate in synchrony with the host cell, they are stably inherited and can be used for long-lasting expression—up to several months both in vitro and in vivo—without modifying the host genome. Use SBI’s CAGs-MCS EEV Vector (Cat.# EEV600A-1) for constitutive expression in most cell types including primary cells and stem cells with the strong CAG promoter.
SBI’s EEV System, which is an enhanced version of the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA1) system, offers:
- High levels of expression
- Easy to clone formats
- No special plasmid production
- Nonviral, non-integrating technology
- Constitutive and inducible vector formats
Please note the following Licensing Restrictions for the EEV system:
For Academic and Non-Profit Institutions:
Researchers at academic and non-profit institutes are granted full access to purchasing the EEV cloning vectors and reporters. Full sequence information is provided upon proof of purchase of EEV vectors. Please contact SBI’s technical support at email@example.com for sequence information.
For Commercial Customers:
For-profit and commercial customers can purchase the pre-made EEV reporters (EEV604A-1, EEV605A-1); however, due to licensing restrictions, cloning EEV vectors (e.g. EEV600A-1 and EEV610A-1) are not available for purchase directly. Instead, these are available through SBI’s custom EEV cloning and production services. SBI then provides the commercial customer with the desired amount of ready-to-use EEV custom construct DNA.