Harness AAV technology with SBI’s AAVanced rAAV Vectors
Widely used for gene therapy development and gene editing in vivo because of their broad tropism, lack of associated disease, the ability to transduce both dividing and non-dividing cells, and long-term transgene expression, recombinant AAV vectors are becoming increasingly popular. To help researchers take advantage of the powerful AAV system, SBI has developed a series of AAV vectors optimized for easy cloning and high titers. The pAAVK-EF1α-MCS-CMV-Puro AAVanced™ Cloning and Expression Vector is an AAV vector that enables the expression of a gene-of-interest from the EF1α promoter and the puromycin resistance marker by the CMV promoter.
Because packaging into the AAV capsid limits the size of AAV vectors, the total amount of DNA between the two ITRs in SBI’s AAVanced Vectors needs to be 5 kb or less.
SBI’s family of AAVanced Cloning and Expression Vectors support a range of projects:
- Optimized for high titer rAAV production
- Available in single and dual promoter formats
- Compatible with any AAV packaging system
- Designed for easy & efficient cloning
- Choose fluorescent or antibiotic selection markers
|AAV503A-1||pAAVK-EF1α-MCS1-CMV-MCS2||None||Flexible—dual promoter, dual MCS|
|AAV526A-1||pAAVK-EF1α-MCS-T2A-EGFP||EGFP||Co-expression with marker|
|AAV527A-1||pAAVK-EF1α-MCS-T2A-Puro||Puro||Co-expression with marker|
|AAV528A-1||pAAVK-EF1α–MCS-T2A-mRFP||mRFP||Co-expression with marker|
|AAV536A-1||pAAVK-EF1α-MCS1-CMV-EGFP||EGFP||Dual promoter with marker|
|AAV537A-1||pAAVK-EF1α-MCS1-CMV-Puro||Puro||Dual promoter with marker|
|AAV538A-1||pAAVK-EF1α-MCS1-CMV-mRFP||mRFP||Dual promoter with marker|