FluoroCet Exosome Quantitation Kit

Get the most sensitive quantitation of exosomes and other extracellular vesicles with this 1 hour, antibody-free assay that has low sample input requirements

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FluoroCet Exosome Quantitation Kit

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FluoroCet Exosome Quantitation Kit

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96 Reactions
FCET96A-1
$ 595
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Overview

Get ultrasensitive exosome quantitation when samples are limiting

Enabling highly sensitive quantitation of exosomes in only 60 minutes, the FluoroCet Kit takes our popular EXOCET assay and increases the sensitivity over 30-fold. Fully compatible with all of SBI’s ExoQuick® exosome isolation reagents as well as ultracentrifugation, FluoroCet is great for quantifying exosomes when samples are limiting.

  • Sensitive
    • Requires as little as 500 ng of exosomal protein
    • Perfect for clinical samples or any situation where samples are limiting
  • Fast—assay is complete in only 60 minutes
  • Versatile—compatible with all major exosome isolation methods (e.g. ExoQuick, ultracentrifugation, ultrafiltration, and immunoffinity capture) from human, mouse, and rat samples
  • Quantitative—internal assay standards enable calculation of exosome abundance

Like EXOCET, FluoroCet measures the activity of acetecylcholinesterase (AChE), a known exosome protein, although the more sensitive FluoroCet assay relies on readout of fluorescence emission at 590-600 nm. To enable calculation of exosome abundance in your target sample, each kit includes standards calibrated to known amounts of human exosomes as determined by NanoSight analysis. The FluoroCet Kit contains all of the reagents needed to perform 96 reactions.

Choose the exosome quantitation method that’s best for your studies

  ExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet
Use For fast and sensitive antibody-based quantitation of exosomes For sensitive quantitation of exosomes when time and input sample are not limiting For fast quantitation of extracellular vesicles with moderate sample input requirements For the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection method Antibody Antibody Enzymatic Enzymatic
Quantitation chemistry Enzymatic (HRP) Enzymatic (HRP) Colorimetric Fluorescent
Total protocol time 4 hours (no overnight incubation) 24 hours 20 min 60 min
Input sample amount (protein equivalent) 1 – 200 µg >500 µg 50 µg <1 µg
Learn More ExoELISA-ULTRA CD63

ExoELISA-ULTRA CD81

ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet

How It Works

Exosome quantitation with FluoroCet is quick and easy

  1. Calibrate your fluorescence plate reader
  2. Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
  3. Lyse exosomes with the included Exosome Lysis Buffer
  4. Measure AChE activity—add the included buffer and incubate for 20 minutes (or appropriate time as determined during the calibration step) at room temperature in the dark, and then measure fluorescence using excitation at 530–570 nm and emission at 590–600 nm.

Supporting Data

The sensitive FluoroCet exosome quantitation assay

Sample data comparing EXOCET’s linear range to FluoroCet’s linear range.

Data from FluoroCet remains linear—and quantitative—even at low exosome amounts. Comparison of limit of detection (LOD) of EXOCET versus FluoroCet.


Product Documentation