XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivector

Label exosomes with luciferase and track uptake into target cells with XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivector.

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XPack CMV-XP-Luciferase-EF1α-PuroStable HEK293 Producer Cell Line

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XPack CMV-XP-Luciferase-EF1α-PuroStable HEK293 Producer Cell Line

[variation_id] => 65995 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
2 x 10^6 Cells
XPAK532CL-1
$ 1182

XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivector

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XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivector

[variation_id] => 65996 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
10 µg
XPAK532PA-1
$ 683

XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivirus

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XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivirus

[variation_id] => 65997 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
>1 x 10^6 IFUs
XPAK532VA-1
$ 683
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Overview

Label exosomes with luciferase using XPackTM

Easily create stable cell lines that produce luciferase-labeled exosomes with the XPack CMV-XP-Luciferase-EF1α-Puro Expression Lentivector. Take advantage of SBI’s well-proven lentivector technology to express luciferase from the strong CMV promoter (also available with an MSCV promoter for expression in hematopoietic and stem cells) and the optimized XPack exosome targeting tag to package luciferase into exosomes.

The XPack System at a glance:

  • Powerful—enables a wealth of applications, including
    • Exosome labeling
    • Exosome cargo tracking
    • Exosomes as cargo delivery vehicles
    • Exosomes as biotherapeutics
    • Exosome-generating stable cell lines with protein cargo-of-interest
  • Optimized—SBI developed the XPack peptide tag for robust exosome targeting
  • Reliable—based on SBI’s highly-cited, well-proven lentivector technology
  • Versatile
    • Two promoter options for expression in a wide range of cells
    • Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors

Choose the XPack Lentivector that’s right for you

  CMV Promoter

For most cell lines

MSCV Promoter

For hematopoietic and stem cell lines

   
Customize with our cloning vector
  XPAK510PA-1 XPack CMV-XP-MCS-EF1α-Puro XPAK710PA-1 XPack MSCV-XP-MCS-EF1α-Puro
Label exosomes with pre-built reporters
GFP XPAK530CL-1

XPAK530PA-1

XPAK530VA-1

XPack CMV-XP-GFP-EF1α-Puro XPAK730PA-1

XPAK730VA-1

XPack MSCV-XP-GFP-EF1α-Puro
RFP XPAK531PA-1 XPack CMV-XP-RFP-EF1α-Puro XPAK731PA-1 XPack MSCV-XP-RFP-EF1α-Puro
Luciferase XPAK532CL-1

XPAK532PA-1

XPAK532VA-1

XPack CMV-XP-Luciferase-EF1α-Puro XPAK732PA-1

XPAK732VA-1

XPack MSCV-XP-Luciferase-EF1α-Puro

How It Works

Optimized for success

With XPack, SBI has developed an optimized N-terminal peptide sequence that targets the peptide—and any protein fused to it—to the inner surface of the exosome membrane. Our pre-built XPack-reporter constructs come in GFP, RFP, and luciferase options to cover a wide range of labeling needs.

Supporting Data

XPack efficiently packages luciferase into exosomes which can be used to deliver GFP to target cells

Part 1: Packaging luciferase into exosomes with XPack

The XPack-Luciferase fusion protein is efficiently packaged as exosome cargo and delivered to target cells. HEK-293 cells were transfected with a plasmid encoding an N-terminal XPack-Luciferase fusion protein (Cat.# XPAK532PA-1). Forty-eight hours later, exosomes were isolated using ExoQuick-TC® and luciferase levels quantified and compared to exosomes isolated from untransfected cells (Figure 1). Only XPack-Luciferase exosomes show robust luminescence indicating efficient packaging of luciferase into exosomes.

XPack efficiently packages luciferase into exosomes

Figure 1. XPack efficiently packages luciferase into exosomes.

Part 2: XPack-Luciferase Exosome Delivery to Target Cells

The XPack-Luciferase exosomes isolated as described above were then added to recipient HEK-293 cells to test for luciferase delivery. After 24-hours, robust luminescence from cells exposed to XPack-Luciferase exosomes shows delivery of the luciferase cargo to the target cells (Figure 2). In contrast, untreated cells or cells treated with exosomes from untransfected cells show no luminescence.XPack-Luciferase exosomes deliver luciferase to target cells

Figure 2. XPack-Luciferase exosomes deliver luciferase to target cells.