The Original ExoQuick

Fast, scalable exosome isolation from plasma, serum, and ascites fluid.

Description
Size
Catalog Number
Price
Quantity
Add to Cart

ExoQuick exosome precipitation solution

20 mL
EXOQ20A-1
$994

ExoQuick exosome precipitation solution

5 mL
EXOQ5A-1
$308

Overview

A better way to isolate exosomes

“We therefore pursued the ExoQuick® method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models1.”
 
Need exosomes? SBI’s ExoQuick, original formulation, enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 100 µl) of serum, plasma, or ascites fluid. Compatible with a wide variety of downstream applications, ExoQuick is an effective and proven alternative to ultracentrifugation1-3.

ExoQuick’s fast, ultracentrifugation-free method:

  • Saves time and labor
  • Is easily scalable
  • Conserves precious sample
  • Delivers high yields of functional, high quality exosomes
  • Can be used to isolate exosomes for a wide range of downstream applications, including
    • Biomarker studies
    • Exosomal miRNA profiling
    • Exosomal proteomics
    • Exosomal lipidomics/metabolomics
    • Functional studies, such as in cell-to-cell signaling
    • Basic biology, such as role in tumorigenesis

ExoQuick is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.

Biofluid Sample volume ExoQuick-TC Volume
Tissue culture media, urine, cerebrospinal fluid (CSF), etc. 5 mL 1 mL

In electron microscopy studies, exosomes isolated with ExoQuick appear similar to exosomes isolated using ultracentrifugation1-2, and these exosomes are also active in numerous functional assays1-3.

Exosomes isolated with ExoQuick can be used for all types of protein profiling and protein characterization studies, such as mass spectrometry, Western blotting, ELISA, and more. Higher protein yields are achieved by ExoQuick purification than by chromatography, DynaBeads, or ultracentrifugation.

Exosomes isolated with ExoQuick also provide excellent samples for studying exosome-associated nucleic acids such as microRNAs, siRNAs, and even mRNA. Quantitative analytical techniques such as qPCR, microarray studies, and next-generation sequencing are all compatible with nucleic acids isolated from ExoQuick-purified exosomes.

Backed by a growing number of publications, ExoQuick is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models.

ExoQuick exosome isolation methods are patented technologies4.

Choose the right ExoQuick for your biofluid:

Catalog # Product Biofluid
EXOQ5A-1, EXOQ20A-1 The Original ExoQuick For serum, plasma, and ascites fluid
EXOTC10A-1, EXOTC50A-1 ExoQuick-TC® For all other biofluids
EXOLP5A-1 ExoQuick-LP For removing contaminating lipoprotein particles from plasma or serum before ExoQuick precipitation
EXOQ5TM-1 ExoQuick Plasma Prep with Thrombin For de-fibrinating plasma before exosome isolation for efficient recovery and high yields
EXOCG50A-1 ExoQuick-CG For preparing exosomes used in pre-clinical in vivo applications
EQPL10A-1, EQPL10TC ExoQuick PLUS and ExoQuick-TC PLUS For sensitive applications such as mass spectrometry, exosome labeling, and in vivo/ex vivo exosome delivery

Choose between ExoQuick/ExoQuick-TC and ExoQuick PLUS/ExoQuick-TC PLUS based on your application

ExoQuick
ExoQuick-TC
ExoQuick PLUS
ExoQuick-TC PLUS

Protein Detection

Western blotting for general exosome markers (e.g. CD9, CD63, CD81, TSG101, Alix) ••••• •••••
High-sensitivity Western blotting (e.g. low abundance biomarkers) ••• •••••

qPCR Analysis

qPCR of coding and non-coding RNAs (e.g. mRNA, miRNA, and lncRNA) ••••• •••••

High-throughput Biomarker Discovery

RNA-seq of exosomal RNAs ••••• •••••
Mass spectrometry of exosomal proteins ••• •••••
Lipidomics/metabolomics of exosomal cargo ••• •••••

Exosome Labeling

••• •••••
In vivo/ex vivo Exosome Delivery ••• •••••

••••• Highly recommended, Not recommended

REFERENCES

  1. Chugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.
  1. Umezu T, et al. Leukemia cell to endothelial cell communication via exosomal miRNAs. Oncogene. 2013 May 30. 32(22):2747-55. PMID: 22797057.
  1. Sohel MM, et al. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence. PLoS One. 2013 Nov 4. 8(11):e78505. PMCID: PMC3817212.
  1. Antes T, et al. Methods for Microvesicle Isolation and Selective Removal. Patent No.: US 9,005,888 B2.

How It Works

High-throughput, quantitative exosome recovery

ExoQuick can be used to purify exosomes from plasma1, serum2, and malignant ascites3. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick is an excellent option for researchers who need to purify multiple exosome samples and/or samples from small animal models or clinical research samples.

To isolate exosomes from cleared serum, plasma, or ascites fluid, simply:

  • Add an appropriate volume of ExoQuick to as little as 100 µl sample
  • Incubate for at least one hour at 4°C
  • Isolate exosomes with a 30-minute low-speed spin (1500 g).

Isolated exosomes can be found in the pellet and resuspended in an appropriate solution.

A quick and easy exosome isolation workflow

You can verify the presence of exosomes with a number of different methods, including Western blotting for general exosome markers (CD63, CD9, CD81, and HSP70), NanoSight analysis, or EM (learn about different ways to detect exosomes and more in our Exosome Basics Guide).

The Bottom Line
With ExoQuick, you can obtain high-quality exosomes from most biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample.

REFERENCES

  1. Chugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.
  1. Epple LM, et al. Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles. PLoS ONE. 2012. 7(7): e42064. PMCID: PMC3407172.
  1. As featured in: Exosome Isolation for Proteomic Analyses and RNA Profiling Douglas D. Taylor, Wolfgang Zacharias and Cicek Gercel-Taylor, Serum/Plasma Proteomics, Methods in Molecular Biology, 2011, Volume 728, Part 4, 235-246.

Supporting Data

Use ExoQuick to isolate exosomes for proteomics and miRNA profiling studies

ExoQuick helps researchers discover protein and RNA biomarkers as well as study exosome biology by enabling fast and quantitative isolation of exosomes.

ExoQuick supports exosomal protein analysis from ascites
 
Exosomes were isolated from ovarian tumor ascites fluid using ExoQuick, chromatography, DynaBeads, or ultracentrifugation. The ExoQuick method consistently delivered higher concentrations of protein than the other three isolation methods used (Figure 1).

ExoQuick delivers high concentrations of exosomal proteins

ExoQuick delivers high concentrations of exosomal proteins

 

Figure 1. (Top panel) Exosomal proteins were extracted from recovered exosomes, and the amount of protein determined by the Bradford microassay method (Bio-Rad Laboratories), using BSA as a standard. Proteins from each exosome isolate were standardized to the original sample volume and equal volumes were applied per lane of a 12.5% SDS-PAGE gel. (Bottom panel) Western Blotting was performed to analyze the presence of the specific marker protein, placental alkaline phosphatase (PLAP). The bound immune complexes were visualized by enhanced chemiluminescence (ECL, Amersham Life Sciences) and quantitated by densitometry (Un-Scan-it Software, Silk Scientific Corp.).

ExoQuick supports high exosomal miRNA yields
 
With ExoQuick, you can quickly and easily isolate high quality exosomes for miRNA analysis (Figure 2).

Exosomal microRNAs were recovered from ovarian tumor ascites fluid using either ExoQuick isolation of exosomes followed by Trizol extraction of RNA, Trizol extraction of ovarian tumor ascites fluid with no exosome isolation, or exosome purification using DynaBeads followed by Trizol extraction of RNA. The samples where exosomes were purified using ExoQuick showed the highest yields of microRNAs (Figure 2).

ExoQuick delivers high yields of exosomal miRNA

Figure 2. Recovered RNA quality and yield was assessed using a GeneQuant II. Small RNAs were analyzed with the Agilent 2100 Bioanalyzer Lab-on-a-Chip instrument system (Agilent Technologies, Santa Clara, CA), using the Agilent Small RNA chip and reagent kit. Approximately 100 ng of isolated total RNA in 1 µl was applied to each run. The manufacturer’s recommended protocol was strictly followed to obtain Bioanalyzer profiles for the size range 6 to 150 nucleotides (nt). The profiles were calibrated for size (nt) using the small RNA ladder supplied with the kit, containing markers of 20, 40, 60, 80, and 150 nt in size, as reference. The instrument software quantitated the peak area between 0 and 150 nt as small RNA region, the area within 10 to 40 nt as miRNA region, and provides percentages of miRNA detected for each sample.

Characterizing ExoQuick exosomes with NanoSight

Exosomes purified with ExoQuick from serum show the expected particle size distribution and high concentration yields when analyzed using NanoSight’s Nanoparticle Tracking Analysis (NTA, Figure 3).

ExoQuick delivers high yields of particles consistent in size with exosomes

Figure 3.Exosome size distribution and yields from serum. Exosomes were purified from 50 pooled samples of normal human serum. 250 µl of serum was combined with 63 µl of ExoQuick, incubated at 4°C for thirty minutes, and pelleted by a 1500g spin for thirty minutes. The exosome pellet was resuspended in 100 µl of PBS, diluted 1:10,000, and visualized on the NanoSight LM10 instrument. The analysis shows that the ExoQuick isolation method recovered 90 nm exosomes at a concentration of of 2.74 x 1012 particles/ml.

Citations

  • Lee, ST, et al. (2017) Exosome-Based Delivery of miR-124 in a Huntington’s Disease Model. J Mov Disord. 2017 Jan 1; 10(1):45-52. PM ID: 28122430
  • Bassil, F, et al. (2017) Insulin resistance and exendin-4 treatment for multiple system atrophy. Brain. 2017 Mar 14;. PM ID: 28334990
  • Caponnetto, F, et al. (2017) Size-dependent cellular uptake of exosomes. Nanomedicine. 2017 Apr 1; 13(3):1011-1020. PM ID: 27993726
  • Huang, Z, et al. (2017) Six Serum-Based miRNAs as Potential Diagnostic Biomarkers for Gastric Cancer. Cancer Epidemiol. Biomarkers Prev.. 2017 Feb 1; 26(2):188-196. PM ID: 27756776
  • Aoki-Yoshida, A, et al. (2017) Exosomes isolated from sera of mice fed Lactobacillus strains affect inflammatory cytokine production in macrophages in vitro. Biochem. Biophys. Res. Commun.. 2017 Jul 22; 489(2):248-254. PM ID: 28559134
  • Fareh, M, et al. (2017) Cell-based therapy using miR-302-367 expressing cells represses glioblastoma growth. Cell Death Dis. 2017 Mar 30; 8(3):e2713. PM ID: 28358371
  • Soung, YH, et al. (2017) Exosomes in cancer diagnostics. Cancers. 2017 Sep 12; 9(1):8. Link: Cancers
  • Zhang, J, et al. (2017) Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes. Proteomics. 2017 Jun 7;. PM ID: 28590090
  • Suehiro, T, et al. (2017) SAT-087 – Significance of serum exosomal miR-122 and miR-21 as a predictive biomarker in hepatocellular carcinoma patients who underwent transarterial chemoembolization. Journal of Hepatology. 2017 May 11; 66(1):S624. Link: Journal of Hepatology
  • Deng, J, et al. (2017) Neurons Export Extracellular Vesicles Enriched in Cysteine String Protein and Misfolded Protein Cargo. Sci Rep. 2017 Apr 19; 7(1):956. PM ID: 28424476
  • Choi, JY, et al. (2017) Extracellular Vesicles as a Source of Urological Biomarkers: Lessons Learned From Advances and Challenges in Clinical Applications to Major Diseases. Int Neurourol J. 2017 Jun 1; 21(2):83-96. PM ID: 28673066
  • Maeda, Y, et al. (2017) Synovium-Derived MicroRNAs Regulate Bone Pathways in Rheumatoid Arthritis. J. Bone Miner. Res.. 2017 Mar 1; 32(3):461-472. PM ID: 27676131
  • Xu, Y, et al. (2017) MiR-145 detection in urinary extracellular vesicles increase diagnostic efficiency of prostate cancer based on hydrostatic filtration dialysis method. Prostate. 2017 Jul 1; 77(10):1167-1175. PM ID: 28617988
  • Devhare, PB, et al. (2017) Exosome mediated intercellular communication between hepatitis C virus infected hepatocytes and hepatic stellate cells. Journal of Virology. 2017 Jan 11;. Link: Journal of Virology
  • DeRita, RM, et al. (2017) c-Src, Insulin-Like Growth Factor I Receptor, G-Protein-Coupled Receptor Kinases and Focal Adhesion Kinase are Enriched Into Prostate Cancer Cell Exosomes. J. Cell. Biochem.. 2017 Jan 1; 118(1):66-73. PM ID: 27232975
  • Abu-Seer, E. (2017) The Reliability of Plasma Exosome Concentrations in Healthy Male Individuals. Journal of Health Science. ; 5:81-94. Link: Journal of Health Science
  • Yoo, J, et al. (2017) AB0236 Association of lyve-1 protein in exosome with disease activity as a new candidate biomarker for rheumatoid arthritis. BMJ Journal. ; 76. Link: BMJ Journal
  • Sami Saribas, A, et al. (2017) HIV-1 Nef is released in extracellular vesicles derived from astrocytes: evidence for Nef-mediated neurotoxicity. Cell Death Dis. 2017 Jan 12; 8(1):e2542. PM ID: 28079886
  • Devhare, PB, et al. (2017) Exosome-Mediated Intercellular Communication between Hepatitis C Virus-Infected Hepatocytes and Hepatic Stellate Cells.. J. Virol.. 2017 Mar 15; 91(6). PM ID: 28077652
  • Chae, MS, et al. (2017) Enhancing surface functionality of reduced graphene oxide biosensors by oxygen plasma treatment for Alzheimer’s disease diagnosis. Biosens Bioelectron. 2017 Jun 15; 92:610-617. PM ID: 27829557