The Original ExoQuick

Fast, scalable exosome isolation from plasma, serum, and ascites fluid.

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ExoQuick exosome precipitation solution

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ExoQuick exosome precipitation solution

[variation_id] => 63798 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
20 mL
EXOQ20A-1
$ 994

ExoQuick exosome precipitation solution

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ExoQuick exosome precipitation solution

[variation_id] => 63799 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
5 mL
EXOQ5A-1
$ 308
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Overview

A better way to isolate exosomes

“We therefore pursued the ExoQuick® method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models1.”
 
Need exosomes? SBI’s ExoQuick, original formulation, enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 100 µl) of serum, plasma, or ascites fluid. Compatible with a wide variety of downstream applications, ExoQuick is an effective and proven alternative to ultracentrifugation1-3.

ExoQuick’s fast, ultracentrifugation-free method:

  • Saves time and labor
  • Is easily scalable
  • Conserves precious sample
  • Delivers high yields of functional, high quality exosomes
  • Can be used to isolate exosomes for a wide range of downstream applications, including
    • Biomarker studies
    • Exosomal miRNA profiling
    • Exosomal proteomics
    • Exosomal lipidomics/metabolomics
    • Functional studies, such as in cell-to-cell signaling
    • Basic biology, such as role in tumorigenesis

ExoQuick is a proprietary polymer that gently precipitates exosomes. Add the appropriate amount of ExoQuick to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.

Biofluid Sample volume ExoQuick Volume
Serum, plasma, and ascites fluid. 250 μl 63 μl

In electron microscopy studies, exosomes isolated with ExoQuick appear similar to exosomes isolated using ultracentrifugation1-2, and these exosomes are also active in numerous functional assays1-3.

Exosomes isolated with ExoQuick can be used for all types of protein profiling and protein characterization studies, such as mass spectrometry, Western blotting, ELISA, and more. Higher protein yields are achieved by ExoQuick purification than by chromatography, DynaBeads, or ultracentrifugation.

Exosomes isolated with ExoQuick also provide excellent samples for studying exosome-associated nucleic acids such as microRNAs, siRNAs, and even mRNA. Quantitative analytical techniques such as qPCR, microarray studies, and next-generation sequencing are all compatible with nucleic acids isolated from ExoQuick-purified exosomes.

Backed by a growing number of publications, ExoQuick is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models.

ExoQuick exosome isolation methods are patented technologies4.

Choose the right ExoQuick for your application:

Application Product Catalog #
Purest EV isolation ExoQuick ULTRA and
ExoQuick-TC ULTRA
EQULTRA-20A-1
EQULTRA-20TC-1
General purpose EV isolation ExoQuick and
ExoQuick-TC
EXOQ20A-1
EXOTC50A-1
EV isolation for pre-clinical/in vivo studies ExoQuick-CG EXOCG50A-1
EV isolation that removes contaminating lipoprotein particles from plasma or serum ExoQuick-LP EXOLP5A-1
EV isolation that includes a de-fibrinating plasma step prior to isolation ExoQuick Plasma Prep with Thrombin EXOQ5TM-1

REFERENCES

  1. Chugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.
  1. Umezu T, et al. Leukemia cell to endothelial cell communication via exosomal miRNAs. Oncogene. 2013 May 30. 32(22):2747-55. PMID: 22797057.
  1. Sohel MM, et al. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence. PLoS One. 2013 Nov 4. 8(11):e78505. PMCID: PMC3817212.
  1. Antes T, et al. Methods for Microvesicle Isolation and Selective Removal. Patent No.: US 9,005,888 B2.

How It Works

High-throughput, quantitative exosome recovery

ExoQuick can be used to purify exosomes from plasma1, serum2, and malignant ascites3. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick is an excellent option for researchers who need to purify multiple exosome samples and/or samples from small animal models or clinical research samples.

To isolate exosomes from cleared serum, plasma, or ascites fluid, simply:

  • Add an appropriate volume of ExoQuick to as little as 100 µl sample
  • Incubate for at least one hour at 4°C
  • Isolate exosomes with a 30-minute low-speed spin (1500 g).

Isolated exosomes can be found in the pellet and resuspended in an appropriate solution.

A quick and easy exosome isolation workflow

You can verify the presence of exosomes with a number of different methods, including Western blotting for general exosome markers (CD63, CD9, CD81, and HSP70), NanoSight analysis, or EM (learn about different ways to detect exosomes and more in our Exosome Basics Guide).

The Bottom Line
With ExoQuick, you can obtain high-quality exosomes from most biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample.

REFERENCES

  1. Chugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.
  1. Epple LM, et al. Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles. PLoS ONE. 2012. 7(7): e42064. PMCID: PMC3407172.
  1. As featured in: Exosome Isolation for Proteomic Analyses and RNA Profiling Douglas D. Taylor, Wolfgang Zacharias and Cicek Gercel-Taylor, Serum/Plasma Proteomics, Methods in Molecular Biology, 2011, Volume 728, Part 4, 235-246.

Supporting Data

Use ExoQuick to isolate exosomes for proteomics and miRNA profiling studies

ExoQuick helps researchers discover protein and RNA biomarkers as well as study exosome biology by enabling fast and quantitative isolation of exosomes.

ExoQuick supports exosomal protein analysis from ascites
 
Exosomes were isolated from ovarian tumor ascites fluid using ExoQuick, chromatography, DynaBeads, or ultracentrifugation. The ExoQuick method consistently delivered higher concentrations of protein than the other three isolation methods used (Figure 1).

ExoQuick delivers high concentrations of exosomal proteins

ExoQuick delivers high concentrations of exosomal proteins

 

Figure 1. (Top panel) Exosomal proteins were extracted from recovered exosomes, and the amount of protein determined by the Bradford microassay method (Bio-Rad Laboratories), using BSA as a standard. Proteins from each exosome isolate were standardized to the original sample volume and equal volumes were applied per lane of a 12.5% SDS-PAGE gel. (Bottom panel) Western Blotting was performed to analyze the presence of the specific marker protein, placental alkaline phosphatase (PLAP). The bound immune complexes were visualized by enhanced chemiluminescence (ECL, Amersham Life Sciences) and quantitated by densitometry (Un-Scan-it Software, Silk Scientific Corp.).

ExoQuick supports high exosomal miRNA yields
 
With ExoQuick, you can quickly and easily isolate high quality exosomes for miRNA analysis (Figure 2).

Exosomal microRNAs were recovered from ovarian tumor ascites fluid using either ExoQuick isolation of exosomes followed by Trizol extraction of RNA, Trizol extraction of ovarian tumor ascites fluid with no exosome isolation, or exosome purification using DynaBeads followed by Trizol extraction of RNA. The samples where exosomes were purified using ExoQuick showed the highest yields of microRNAs (Figure 2).

ExoQuick delivers high yields of exosomal miRNA

Figure 2. Recovered RNA quality and yield was assessed using a GeneQuant II. Small RNAs were analyzed with the Agilent 2100 Bioanalyzer Lab-on-a-Chip instrument system (Agilent Technologies, Santa Clara, CA), using the Agilent Small RNA chip and reagent kit. Approximately 100 ng of isolated total RNA in 1 µl was applied to each run. The manufacturer’s recommended protocol was strictly followed to obtain Bioanalyzer profiles for the size range 6 to 150 nucleotides (nt). The profiles were calibrated for size (nt) using the small RNA ladder supplied with the kit, containing markers of 20, 40, 60, 80, and 150 nt in size, as reference. The instrument software quantitated the peak area between 0 and 150 nt as small RNA region, the area within 10 to 40 nt as miRNA region, and provides percentages of miRNA detected for each sample.

Characterizing ExoQuick exosomes with NanoSight

Exosomes purified with ExoQuick from serum show the expected particle size distribution and high concentration yields when analyzed using NanoSight’s Nanoparticle Tracking Analysis (NTA, Figure 3).

ExoQuick delivers high yields of particles consistent in size with exosomes

Figure 3.Exosome size distribution and yields from serum. Exosomes were purified from 50 pooled samples of normal human serum. 250 µl of serum was combined with 63 µl of ExoQuick, incubated at 4°C for thirty minutes, and pelleted by a 1500g spin for thirty minutes. The exosome pellet was resuspended in 100 µl of PBS, diluted 1:10,000, and visualized on the NanoSight LM10 instrument. The analysis shows that the ExoQuick isolation method recovered 90 nm exosomes at a concentration of of 2.74 x 1012 particles/ml.


Product Documentation

Citations

  • Elshelmani, H & Rani, S. (2017) Exosomal MicroRNA Discovery in Age-Related Macular Degeneration. Methods Mol. Biol.. 2017 Nov 9; 1509:93-113. PM ID: 27826921
  • Soung, YH, et al. (2017) Exosomes in cancer diagnostics. Cancers. 2017 Sep 12; 9(1):8. Link: Cancers
  • Thakur, A, et al. (2017) Direct detection of two different tumor-derived extracellular vesicles by SAM-AuNIs LSPR biosensor. Biosens Bioelectron. 2017 Aug 15; 94:400-407. PM ID: 28324860
  • Mrowczynski, OD, et al. (2017) HFE genotype affects exosome phenotype in cancer. Biochim. Biophys. Acta. 2017 Aug 1; 1861(8):1921-1928. PM ID: 28527894
  • Aoki-Yoshida, A, et al. (2017) Exosomes isolated from sera of mice fed Lactobacillus strains affect inflammatory cytokine production in macrophages in vitro. Biochem. Biophys. Res. Commun.. 2017 Jul 22; 489(2):248-254. PM ID: 28559134
  • Belo, R, et al. (2017) Leishmania infantum Exoproducts Inhibit Human Invariant NKT Cell Expansion and Activation. Front Immunol. 2017 Jul 4; 8:710. PM ID: 28674535
  • Xu, Y, et al. (2017) MiR-145 detection in urinary extracellular vesicles increase diagnostic efficiency of prostate cancer based on hydrostatic filtration dialysis method. Prostate. 2017 Jul 1; 77(10):1167-1175. PM ID: 28617988
  • Syn, NL, et al. (2017) Exosomes in Cancer Nanomedicine and Immunotherapy: Prospects and Challenges. Trends Biotechnol.. 2017 Jul 1; 35(7):665-676. PM ID: 28365132
  • Barreiro, K & Holthofer, H. (2017) Urinary extracellular vesicles. A promising shortcut to novel biomarker discoveries. Cell Tissue Res.. 2017 Jul 1; 369(1):217-227. PM ID: 28429073
  • Ye, W, et al. (2017) Plasma-derived exosomes contribute to inflammation via the TLR9-NF-κB pathway in chronic heart failure patients. Mol. Immunol.. 2017 Jul 1; 87:114-121. PM ID: 28433888
  • Chen, X, et al. (2017) Exosomes derived from hypoxic epithelial ovarian cancer deliver microRNA-940 to induce macrophage M2 polarization. Oncol. Rep.. 2017 Jul 1; 38(1):522-528. PM ID: 28586039
  • Cho, YE, et al. (2017) Circulating Plasma and Exosomal microRNAs as Indicators of Drug-Induced Organ Injury in Rodent Models. Biomol Ther (Seoul). 2017 Jul 1; 25(4):367-373. PM ID: 28208010
  • Wang, J, et al. (2017) Exosomes: A Novel Strategy for Treatment and Prevention of Diseases. Front Pharmacol. 2017 Jun 29; 8:300. PM ID: 28659795
  • Barteneva, NS, et al. (2017) Extracellular vesicles in gastrointestinal cancer in conjunction with microbiota: On the border of Kingdoms. Biochim. Biophys. Acta. 2017 Jun 29;. PM ID: 28669749
  • Tian, F, et al. (2017) No Significant Difference between Plasma miRNAs and Plasma-Derived Exosomal miRNAs from Healthy People. Biomed Res Int. 2017 Jun 28; 2017:1304816. PM ID: 28656135
  • Cheng, L, et al. (2017) Exosomes from Melatonin Treated Hepatocellularcarcinoma Cells Alter the Immunosupression Status through STAT3 Pathway in Macrophages. Int. J. Biol. Sci.. 2017 Jun 28; 13(6):723-734. PM ID: 28655998
  • Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017 Jun 27; 8(26):42712-42727. PM ID: 28514744
  • Sempere, LF, Keto, J & Fabbri, M. (2017) Exosomal MicroRNAs in Breast Cancer towards Diagnostic and Therapeutic Applications. Cancers (Basel). 2017 Jun 24; 9(7). PM ID: 28672799
  • Wang, J, et al. (2017) Circulating exosomal miR-125a-3p as a novel biomarker for early-stage colon cancer. Sci Rep. 2017 Jun 23; 7(1):4150. PM ID: 28646161
  • Lee, YS, et al. (2017) Exosomes derived from palmitic acid-treated hepatocytes induce fibrotic activation of hepatic stellate cells. Sci Rep. 2017 Jun 16; 7(1):3710. PM ID: 28623272