Exo-Fect™ Exosome Transfection Kit

Turn your exosomes into delivery vehicles with Exo-Fect—enables insertion of RNAs, DNAs (including plasmids), and small molecules into isolated exosomes.
  • Easy-to-use with a fast and straightforward loading protocol
  • Introduces a wide range of biomolecules directly into isolated exosomes:
    • RNAs, including siRNAs, miRNAs, and mRNAs
    • DNAs, including plasmids
    • Metabolites and other small molecules

Products

Catalog Number Description Size Price Quantity Add to Cart
EXFT10A-1 Exo-Fect Exosome Transfection Kit 10 Reactions $238
- +
EXFT20A-1 Exo-Fect Exosome Transfection Kit 20 Reactions $429
- +

Overview

Overview

Putting exosomes to work: Load your cargo directly into isolated exosomes With Exo-Fect, you can turn isolated exosomes into cargo delivery vehicles that introduce RNAs, DNAs (including plasmids), and even small molecules into recipient cells. Simply combine isolated exosomes with Exo-Fect and your desired cargo, and in less than an hour your loaded exosomes will be ready to add to recipient cells. Exo-Fect turns your exosomes into cargo delivery vehicles
  • Easy-to-use with a fast and straightforward loading protocol
  • Introduces a wide range of biomolecules directly into isolated exosomes:
    • RNAs, including siRNAs, miRNAs, and mRNAs
    • DNAs, including plasmids
    • Metabolites and other small molecules
  • Enables non-viral transduction and stable cell line creation
  • Provides an alternative gene delivery method for hard-to-transfect cells
All Exo-Fect Kits come with a Texas Red-labeled positive control, non-targeting siRNA to confirm exosome transfections. Pair with our EV-Entry Reagent and maximize cargo uptake by recipient cells.

How It Works

How It Works

Quickly and easily load cargo into isolated exosomes

Simply combine the isolated exosomes with Exo-Fect Reagent and cargo, perform two incubations for a total of 40 minutes, and then pellet your exosomes. The Kit comes with all of the reagents you need to load cargo into exosomes and concentrate them for delivery to target cells. The protocol takes less than an hour and is highly efficient at loading cargo into exosomes for transport and delivery.

Exo-Fect is also compatible with the EV-Entry Reagent, which maximizes cargo uptake by recipient cells.

Supporting Data

Supporting Data

See how Exo-Fect can be used to create cargo-delivering exosomes

Exo-Fect efficiently loads small RNAs into exosomes for delivery to recipient cells
 
Exo-Fect efficiently loads siRNA into exosomes.

Figure 1. Exo-Fect efficiently loads siRNA into exosomes. Fluorescence imaging shows exosomes that were loaded with either a Texas Red end-labeled siRNA (left panels), or unlabeled siRNA (right panels) using Exo-Fect, and then immobilized onto Exo-Flow™ CD63 magnetic beads.

Exo-Fect transfected exosomes deliver siRNA cargo to recipient cells

Figure 2. Exo-Fect transfected exosomes deliver siRNA cargo to recipient cells. The siRNA-loaded exosomes from Figure 1 were eluted from the Exo-Flow CD63 magnetic beads and then added to HEK293 cells. Fluorescence of the recipient cells indicates that the Texas Red-labeled siRNA cargo was successfully delivered.

Exo-Fect efficiently loads mRNAs and DNA plasmids into exosomes for delivery to recipient cells
 Exo-Fect loaded exosomes deliver mRNA and plasmid DNA into recipient cells

Figure 3. Exo-Fect loaded exosomes deliver mRNA and plasmid DNA into recipient cells. ExoFect Kits also work with larger nucleic acids, like mRNAs and plasmids. (Left panels) We used Exo-Fect to transfect 1 µg of an mRNA encoding RFP into exosomes. These exosomes were then added to HEK293 cells and imaged for RFP protein production after 24 hours. (Right panels) We used Exo-Fect to transfect 5 µg of plasmid DNA encoding a GFP gene into exosomes, and then added the plasmid-loaded exosomes to HEK293 cells. The cells were imaged for GFP protein presence after 48 hours. The appearance of RFP signal (mRNA cargo) and GFP signal (plasmid cargo) in recipient cells indicates successful cargo delivery.

Exo-Fect efficiently loads small molecules into exosomes for delivery to recipient cellsExo-Fect transfects the small molecule cumate into exosomesExo-Fect transfects the small molecule cumate into exosomes

Figure 4. Exo-Fect can even be used to transfect small molecules into exosomes. We loaded cumate into exosomes using Exo-Fect, and then added loaded exosomes to cells containing a cumate-inducible GFP reporter construct. Only cumate-containing exosomes were able to induce GFP expression in recipient cells, indicating successful delivery of cumate.

FAQs

Resources

Citations

  • Ellipilli, S, et al. (2023) Ligand-displaying-exosomes using RNA nanotechnology for targeted delivery of multi-specific drugs for liver cancer regression. Nanomedicine : nanotechnology, biology, and medicine. 2023; 50:102667. PM ID: 36948369
  • Lu, Y, et al. (2023) Exosome-Based Carrier for RNA Delivery: Progress and Challenges. Pharmaceutics. 2023; 15(2). PM ID: 36839920
  • Zhao, W, et al. (2023) Accelerating corneal wound healing using exosome-mediated targeting of NF-κB c-Rel. Inflammation and regeneration. 2023; 43(1):6. PM ID: 36703231
  • Lin, W, et al. (2023) Tumor-intrinsic YTHDF1 drives immune evasion and resistance to immune checkpoint inhibitors via promoting MHC-I degradation. Nature communications. 2023; 14(1):265. PM ID: 36650153
  • Lang, HL, et al. (2023) Small extracellular vesicles secreted by induced pluripotent stem cell-derived mesenchymal stem cells improve postoperative cognitive dysfunction in mice with diabetes. Neural regeneration research. 2023; 18(3):609-617. PM ID: 36018185
  • Cao, H, et al. (2022) Extracellular Vesicles-Encapsulated miR-153-3p Potentiate the Survival and Invasion of Lung Adenocarcinoma. Molecules and cells. 2022; 45(6):376-387. PM ID: 35611687
  • Jayasinghe, M, et al. (2022) Surface-engineered extracellular vesicles for targeted delivery of therapeutic RNAs and peptides for cancer therapy. Theranostics. 2022; 12(7):3288-3315. Link: Theranostics
  • Gong, C, et al. (2022) HMSCs exosome-derived miR-199a-5p attenuates sulfur mustard-associated oxidative stress via the CAV1/NRF2 signaling pathway. Research Square. 2022;. Link: Research Square
  • Esteves, M, et al. (2022) MicroRNA-124-3p-enriched small extracellular vesicles as a therapeutic approach for Parkinson’s disease. Molecular therapy : the journal of the American Society of Gene Therapy. 2022;. PM ID: 35689381
  • Sheng, C, et al. (2022) Exosomes derived from human adipose-derived stem cells ameliorate osteoporosis through miR-335-3p/Aplnr axis. Nano Research. 2022;. Link: Nano Research
  • Wang, C, et al. (2022) Nicotine exacerbates endothelial dysfunction and drives atherosclerosis via extracellular vesicle-miRNA. Cardiovascular research. 2022;. PM ID: 36006370
  • Nordmeier, S, Hsiung, F & Portnoy, V. (2022) Extracellular Vesicles (EVs). RNA Nanotechnology and Therapeutics. 2022;:377-382. Link: RNA Nanotechnology and Therapeutics
  • Lei, D, et al. (2022) Hypoxia-elicited cardiac microvascular endothelial cell-derived exosomal miR-210-3p alleviate hypoxia/reoxygenation-induced myocardial cell injury through inhibiting transferrin receptor 1-mediated ferroptosis. Tissue & cell. 2022; 79:101956. PM ID: 36272206
  • Dong, Z, et al. (2022) Circulating Small Extracellular Vesicle-Derived miR-342-5p Ameliorates Beta-Amyloid Formation via Targeting Beta-site APP Cleaving Enzyme 1 in Alzheimer’s Disease. Cells. 2022; 11(23):3830. Link: Cells
  • Wu, Y, et al. (2022) Osteoclast-derived extracellular miR-106a-5p promotes osteogenic differentiation and facilitates bone defect healing. Cellular Signalling. 2022;:110549. Link: Cellular Signalling
  • Pei, X, et al. (2021) Targeted exosomes for co-delivery of siFGL1 and siTGF-β1 trigger combined cancer immunotherapy by remodeling immunosuppressive tumor microenvironment. Chemical Engineering Journal. 2021; 421:129774. Link: Chemical Engineering Journal
  • Yee Mon, KJ, et al. (2021) MicroRNA-29 specifies age-related differences in the CD8+ T cell immune response. Cell reports. 2021; 37(6):109969. PM ID: 34758312
  • Gunassekaran, GR, et al. (2021) M1 macrophage exosomes engineered to foster M1 polarization and target the IL-4 receptor inhibit tumor growth by reprogramming tumor-associated macrophages into M1-like macrophages. Biomaterials. 2021; 278:121137. PM ID: 34560422
  • McAndrews, KM, et al. (2021) Exosome-mediated delivery of CRISPR/Cas9 for targeting of oncogenic KrasG12D in pancreatic cancer. Life science alliance. 2021; 4(9). PM ID: 34282051
  • Xun, J, et al. (2021) Serum exosomes from young rats improve the reduced osteogenic differentiation of BMSCs in aged rats with osteoporosis after fatigue loading in vivo. Stem cell research & therapy. 2021; 12(1):424. PM ID: 34315544

Products

Catalog Number Description Size Price Quantity Add to Cart
EXFT10A-1 Exo-Fect Exosome Transfection Kit 10 Reactions $238
- +
EXFT20A-1 Exo-Fect Exosome Transfection Kit 20 Reactions $429
- +

Overview

Overview

Putting exosomes to work: Load your cargo directly into isolated exosomes With Exo-Fect, you can turn isolated exosomes into cargo delivery vehicles that introduce RNAs, DNAs (including plasmids), and even small molecules into recipient cells. Simply combine isolated exosomes with Exo-Fect and your desired cargo, and in less than an hour your loaded exosomes will be ready to add to recipient cells. Exo-Fect turns your exosomes into cargo delivery vehicles
  • Easy-to-use with a fast and straightforward loading protocol
  • Introduces a wide range of biomolecules directly into isolated exosomes:
    • RNAs, including siRNAs, miRNAs, and mRNAs
    • DNAs, including plasmids
    • Metabolites and other small molecules
  • Enables non-viral transduction and stable cell line creation
  • Provides an alternative gene delivery method for hard-to-transfect cells
All Exo-Fect Kits come with a Texas Red-labeled positive control, non-targeting siRNA to confirm exosome transfections. Pair with our EV-Entry Reagent and maximize cargo uptake by recipient cells.

How It Works

How It Works

Quickly and easily load cargo into isolated exosomes

Simply combine the isolated exosomes with Exo-Fect Reagent and cargo, perform two incubations for a total of 40 minutes, and then pellet your exosomes. The Kit comes with all of the reagents you need to load cargo into exosomes and concentrate them for delivery to target cells. The protocol takes less than an hour and is highly efficient at loading cargo into exosomes for transport and delivery.

Exo-Fect is also compatible with the EV-Entry Reagent, which maximizes cargo uptake by recipient cells.

Supporting Data

Supporting Data

See how Exo-Fect can be used to create cargo-delivering exosomes

Exo-Fect efficiently loads small RNAs into exosomes for delivery to recipient cells
 
Exo-Fect efficiently loads siRNA into exosomes.

Figure 1. Exo-Fect efficiently loads siRNA into exosomes. Fluorescence imaging shows exosomes that were loaded with either a Texas Red end-labeled siRNA (left panels), or unlabeled siRNA (right panels) using Exo-Fect, and then immobilized onto Exo-Flow™ CD63 magnetic beads.

Exo-Fect transfected exosomes deliver siRNA cargo to recipient cells

Figure 2. Exo-Fect transfected exosomes deliver siRNA cargo to recipient cells. The siRNA-loaded exosomes from Figure 1 were eluted from the Exo-Flow CD63 magnetic beads and then added to HEK293 cells. Fluorescence of the recipient cells indicates that the Texas Red-labeled siRNA cargo was successfully delivered.

Exo-Fect efficiently loads mRNAs and DNA plasmids into exosomes for delivery to recipient cells
 Exo-Fect loaded exosomes deliver mRNA and plasmid DNA into recipient cells

Figure 3. Exo-Fect loaded exosomes deliver mRNA and plasmid DNA into recipient cells. ExoFect Kits also work with larger nucleic acids, like mRNAs and plasmids. (Left panels) We used Exo-Fect to transfect 1 µg of an mRNA encoding RFP into exosomes. These exosomes were then added to HEK293 cells and imaged for RFP protein production after 24 hours. (Right panels) We used Exo-Fect to transfect 5 µg of plasmid DNA encoding a GFP gene into exosomes, and then added the plasmid-loaded exosomes to HEK293 cells. The cells were imaged for GFP protein presence after 48 hours. The appearance of RFP signal (mRNA cargo) and GFP signal (plasmid cargo) in recipient cells indicates successful cargo delivery.

Exo-Fect efficiently loads small molecules into exosomes for delivery to recipient cellsExo-Fect transfects the small molecule cumate into exosomesExo-Fect transfects the small molecule cumate into exosomes

Figure 4. Exo-Fect can even be used to transfect small molecules into exosomes. We loaded cumate into exosomes using Exo-Fect, and then added loaded exosomes to cells containing a cumate-inducible GFP reporter construct. Only cumate-containing exosomes were able to induce GFP expression in recipient cells, indicating successful delivery of cumate.

FAQs

Citations

  • Ellipilli, S, et al. (2023) Ligand-displaying-exosomes using RNA nanotechnology for targeted delivery of multi-specific drugs for liver cancer regression. Nanomedicine : nanotechnology, biology, and medicine. 2023; 50:102667. PM ID: 36948369
  • Lu, Y, et al. (2023) Exosome-Based Carrier for RNA Delivery: Progress and Challenges. Pharmaceutics. 2023; 15(2). PM ID: 36839920
  • Zhao, W, et al. (2023) Accelerating corneal wound healing using exosome-mediated targeting of NF-κB c-Rel. Inflammation and regeneration. 2023; 43(1):6. PM ID: 36703231
  • Lin, W, et al. (2023) Tumor-intrinsic YTHDF1 drives immune evasion and resistance to immune checkpoint inhibitors via promoting MHC-I degradation. Nature communications. 2023; 14(1):265. PM ID: 36650153
  • Lang, HL, et al. (2023) Small extracellular vesicles secreted by induced pluripotent stem cell-derived mesenchymal stem cells improve postoperative cognitive dysfunction in mice with diabetes. Neural regeneration research. 2023; 18(3):609-617. PM ID: 36018185
  • Cao, H, et al. (2022) Extracellular Vesicles-Encapsulated miR-153-3p Potentiate the Survival and Invasion of Lung Adenocarcinoma. Molecules and cells. 2022; 45(6):376-387. PM ID: 35611687
  • Jayasinghe, M, et al. (2022) Surface-engineered extracellular vesicles for targeted delivery of therapeutic RNAs and peptides for cancer therapy. Theranostics. 2022; 12(7):3288-3315. Link: Theranostics
  • Gong, C, et al. (2022) HMSCs exosome-derived miR-199a-5p attenuates sulfur mustard-associated oxidative stress via the CAV1/NRF2 signaling pathway. Research Square. 2022;. Link: Research Square
  • Esteves, M, et al. (2022) MicroRNA-124-3p-enriched small extracellular vesicles as a therapeutic approach for Parkinson’s disease. Molecular therapy : the journal of the American Society of Gene Therapy. 2022;. PM ID: 35689381
  • Sheng, C, et al. (2022) Exosomes derived from human adipose-derived stem cells ameliorate osteoporosis through miR-335-3p/Aplnr axis. Nano Research. 2022;. Link: Nano Research
  • Wang, C, et al. (2022) Nicotine exacerbates endothelial dysfunction and drives atherosclerosis via extracellular vesicle-miRNA. Cardiovascular research. 2022;. PM ID: 36006370
  • Nordmeier, S, Hsiung, F & Portnoy, V. (2022) Extracellular Vesicles (EVs). RNA Nanotechnology and Therapeutics. 2022;:377-382. Link: RNA Nanotechnology and Therapeutics
  • Lei, D, et al. (2022) Hypoxia-elicited cardiac microvascular endothelial cell-derived exosomal miR-210-3p alleviate hypoxia/reoxygenation-induced myocardial cell injury through inhibiting transferrin receptor 1-mediated ferroptosis. Tissue & cell. 2022; 79:101956. PM ID: 36272206
  • Dong, Z, et al. (2022) Circulating Small Extracellular Vesicle-Derived miR-342-5p Ameliorates Beta-Amyloid Formation via Targeting Beta-site APP Cleaving Enzyme 1 in Alzheimer’s Disease. Cells. 2022; 11(23):3830. Link: Cells
  • Wu, Y, et al. (2022) Osteoclast-derived extracellular miR-106a-5p promotes osteogenic differentiation and facilitates bone defect healing. Cellular Signalling. 2022;:110549. Link: Cellular Signalling
  • Pei, X, et al. (2021) Targeted exosomes for co-delivery of siFGL1 and siTGF-β1 trigger combined cancer immunotherapy by remodeling immunosuppressive tumor microenvironment. Chemical Engineering Journal. 2021; 421:129774. Link: Chemical Engineering Journal
  • Yee Mon, KJ, et al. (2021) MicroRNA-29 specifies age-related differences in the CD8+ T cell immune response. Cell reports. 2021; 37(6):109969. PM ID: 34758312
  • Gunassekaran, GR, et al. (2021) M1 macrophage exosomes engineered to foster M1 polarization and target the IL-4 receptor inhibit tumor growth by reprogramming tumor-associated macrophages into M1-like macrophages. Biomaterials. 2021; 278:121137. PM ID: 34560422
  • McAndrews, KM, et al. (2021) Exosome-mediated delivery of CRISPR/Cas9 for targeting of oncogenic KrasG12D in pancreatic cancer. Life science alliance. 2021; 4(9). PM ID: 34282051
  • Xun, J, et al. (2021) Serum exosomes from young rats improve the reduced osteogenic differentiation of BMSCs in aged rats with osteoporosis after fatigue loading in vivo. Stem cell research & therapy. 2021; 12(1):424. PM ID: 34315544