EVery cDNA Synthesis Kit
- Get quick (<2 hrs) and easy cDNA synthesis optimized for use with the EVery EV RNA Isolation Kit
- Find low abundance RNAs with the optional pre-amplification step
- Use with most downstream applications such as PCR, qPCR and miRNA profiling
- Includes sufficient Universal reverse primer for all 8 profiles from EVery miRNome Profiler for human Serum and Plasma
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EVery200B-1 | EVery cDNA Synthesis Kit | 20 Preps | $318 |
|
||||
Overview
Overview
Accelerate your EV biomarker studies
Optimized for use with the EVery EV RNA Isolation Kit, the EVery cDNA Synthesis Kit provides high-quality exosomal cDNA for your downstream applications such as PCR, qPCR, miRNA profiling, and RNA-seq. The kit generates cDNA through poly(A) tailing of exoRNA followed by reverse transcription with MMLV Reverse Transcriptase.
The optimized Universal reverse primer, included in the kit, will anneal efficiently to the adaptor sequence incorporated at the 3’ end of cDNA. This can be used for PCR amplification of desired RNA(s) along with specific forward primer(s) annealing to the RNA(s) of interest.
- Get quick (<2 hrs) and easy cDNA synthesis optimized for use with the EVery EV RNA Isolation Kit
- Find low abundance RNAs with the optional pre-amplification step
- Use with most downstream applications such as PCR, qPCR and miRNA profiling
- Includes sufficient Universal reverse primer for all 8 profiles from EVery miRNome Profiler for human Serum and Plasma
The EVery cDNA Synthesis Kit comes with sufficient reagents to perform 20 purification reactions. EVery EV RNA technology is also available bundled with SBI's powerful EV isolation technologies as well as EVery cDNA Synthesis Kit (Table 1).
Table 1. EVery EV RNA Isolation and cDNA Synthesis Products
| Cat. # | Product description |
|---|---|
| EVery100B-1 | EVery EV RNA Isolation Kit |
| EVery200B-1 | EVery cDNA Synthesis Kit |
How It Works
Supporting Data
Supporting Data
Get robust cDNA synthesis with EVery EV RNA
To demonstrate the excellent RNA yields and robust cDNA synthesis obtained with EVery EV RNA, we isolated EVs from 250 µL of serum using SmartSEC Single, spiked in 0.1 pmol of Cel-miR-39, and used both the EVery EV RNA Isolation Kit and a phenol-based kit to isolate RNA. The isolated RNA was reverse transcribed using the EVery cDNA Synthesis Kit and the copy number of Cel-miR-39 measured (Figure 1). The EVery EV RNA Isolation Kit delivered similar levels of Cel-miR-39 as the phenol-based method.
Figure 1. Every EV RNA is EVery bit as good as phenol.
EVery EV RNA efficiently isolates mRNA
We used the EVery EV RNA Isolation Kit and EVery cDNA Synthesis Kit to isolate mRNA and synthesize cDNA from cells overexpressing eGFP (Figure 2). Robust levels of eGFP mRNA are recovered and converted to cDNA when cells are overexpressing eGFP.
Figure 2. EVery EV RNA efficiently isolates longer RNAs like mRNA.
miRNA isolated and converted to cDNA using EVery EV RNA can be used for miRNA profiling
We isolated EVs from 250 µL of normal or breast cancer patient serum using SmartSEC Single and used EVery EV RNA Isolation Kit and EVery cDNA Synthesis Kit to isolate and reverse transcribe EV RNAs for miRNA profiling using EVery miRNome Profiler for Human Serum and Plasma (EVery500B-1). Distinct miRNA expression profiles of breast cancer patient serum compared to normal serum are shown in Figure 3. Differential expression of selected breast cancer associated miRNA markers were further confirmed by qPCR (Figure 4).
Figure 3. EVery family of products generate high-quality cDNA suitable for miRNA profiling.
Figure 4. Differential expression of selected miRNAs in normal vs. breast cancer serum confirmed by qPCR. Data normalized with global mean. Expression of miR-451a, miR-21-5p, miR-16-5p and miR-103a are significantly upregulated, while expression of the tumor suppressor miR-124-3p is significantly downregulated in breast cancer serum compared to normal serum.
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Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EVery200B-1 | EVery cDNA Synthesis Kit | 20 Preps | $318 |
|
||||
Overview
Overview
Accelerate your EV biomarker studies
Optimized for use with the EVery EV RNA Isolation Kit, the EVery cDNA Synthesis Kit provides high-quality exosomal cDNA for your downstream applications such as PCR, qPCR, miRNA profiling, and RNA-seq. The kit generates cDNA through poly(A) tailing of exoRNA followed by reverse transcription with MMLV Reverse Transcriptase.
The optimized Universal reverse primer, included in the kit, will anneal efficiently to the adaptor sequence incorporated at the 3’ end of cDNA. This can be used for PCR amplification of desired RNA(s) along with specific forward primer(s) annealing to the RNA(s) of interest.
- Get quick (<2 hrs) and easy cDNA synthesis optimized for use with the EVery EV RNA Isolation Kit
- Find low abundance RNAs with the optional pre-amplification step
- Use with most downstream applications such as PCR, qPCR and miRNA profiling
- Includes sufficient Universal reverse primer for all 8 profiles from EVery miRNome Profiler for human Serum and Plasma
The EVery cDNA Synthesis Kit comes with sufficient reagents to perform 20 purification reactions. EVery EV RNA technology is also available bundled with SBI's powerful EV isolation technologies as well as EVery cDNA Synthesis Kit (Table 1).
Table 1. EVery EV RNA Isolation and cDNA Synthesis Products
| Cat. # | Product description |
|---|---|
| EVery100B-1 | EVery EV RNA Isolation Kit |
| EVery200B-1 | EVery cDNA Synthesis Kit |
How It Works
Supporting Data
Supporting Data
Get robust cDNA synthesis with EVery EV RNA
To demonstrate the excellent RNA yields and robust cDNA synthesis obtained with EVery EV RNA, we isolated EVs from 250 µL of serum using SmartSEC Single, spiked in 0.1 pmol of Cel-miR-39, and used both the EVery EV RNA Isolation Kit and a phenol-based kit to isolate RNA. The isolated RNA was reverse transcribed using the EVery cDNA Synthesis Kit and the copy number of Cel-miR-39 measured (Figure 1). The EVery EV RNA Isolation Kit delivered similar levels of Cel-miR-39 as the phenol-based method.
Figure 1. Every EV RNA is EVery bit as good as phenol.
EVery EV RNA efficiently isolates mRNA
We used the EVery EV RNA Isolation Kit and EVery cDNA Synthesis Kit to isolate mRNA and synthesize cDNA from cells overexpressing eGFP (Figure 2). Robust levels of eGFP mRNA are recovered and converted to cDNA when cells are overexpressing eGFP.
Figure 2. EVery EV RNA efficiently isolates longer RNAs like mRNA.
miRNA isolated and converted to cDNA using EVery EV RNA can be used for miRNA profiling
We isolated EVs from 250 µL of normal or breast cancer patient serum using SmartSEC Single and used EVery EV RNA Isolation Kit and EVery cDNA Synthesis Kit to isolate and reverse transcribe EV RNAs for miRNA profiling using EVery miRNome Profiler for Human Serum and Plasma (EVery500B-1). Distinct miRNA expression profiles of breast cancer patient serum compared to normal serum are shown in Figure 3. Differential expression of selected breast cancer associated miRNA markers were further confirmed by qPCR (Figure 4).
Figure 3. EVery family of products generate high-quality cDNA suitable for miRNA profiling.
Figure 4. Differential expression of selected miRNAs in normal vs. breast cancer serum confirmed by qPCR. Data normalized with global mean. Expression of miR-451a, miR-21-5p, miR-16-5p and miR-103a are significantly upregulated, while expression of the tumor suppressor miR-124-3p is significantly downregulated in breast cancer serum compared to normal serum.