ExoGlow™-Protein EV Labeling Kit (Blue)

Label EV proteins blue (403 nm excitation/454 nm emission) with a reagent specifically optimized for EV labeling.
  • Specific—carefully developed to generate a robust signal specific for internal EV proteins, leading to very low levels of background
  • Compatible—delivers robust performance on EVs isolated using all methods tested—including ExoQuick™, ultracentrifugation, and column-based workflows
  • Easy-to-use—labeling protocol is quick and straightforward
  • Powerful—can be used with as little as 200 µg of EVs

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOGP400A-1 ExoGlowTM-Protein EV Labeling Kit (Blue) 20 Reactions $340
- +

Overview

Overview

See EVsbetter with reagents specifically optimized for labeling

SBI’s new generation of extracellular vesicle (EV) labeling reagents take your EV visualization to new levels of clarity, with low background and high selectivity. Unlike general-purpose labeling reagents that are not optimized for EVs and suffer from high levels of background signal, ExoGlow-Protein Blue improves your ability to track and localize EVs by specifically and covalently labeling internal EV proteins with a proprietary blue fluorescent dye that delivers very low levels of background signal. The result is unmatched EV imaging for more accurate studies (see below for our full range of next generation ExoGlow reagents).

  • Specific—carefully developed to generate a robust signal specific for internal EV proteins, leading to very low levels of background
  • Compatible—delivers robust performance on EVs isolated using all methods tested—including ExoQuick, ultracentrifugation, and column-based workflows
  • Easy-to-use—labeling protocol is quick and straightforward
  • Powerful—can be used with as little as 200 µg of EVs
ExoGlow-Protein Blue properties:
Excitation: 403 nm
Emission: 454 nm
Laser line: 405 nm
Available next generation ExoGlow EV imaging reagents
EV Component LabeledCat. #ProductExcitation (nm)Emission (nm)Laser Line (nm)
Internal proteinEXOGP100A-1ExoGlow-Protein EV Labeling Kit (Red)573
588561
EXOGP300A-1ExoGlow-Protein EV Labeling Kit (Green)

511525488
EXOGP400A-1ExoGlow-Protein EV Labeling Kit (Blue)403454
405
RNAEXOGR800A-1ExoGlow-RNA EV Labeling Kit485537488
MembraneEXOGM600A-1ExoGlow-Membrane EV Labeling Kit465635488

How It Works

Supporting Data

Supporting Data

Imaging EVs with a new level of clarity

ExoGlow-Protein enables clear visualization of labeled EVs being internalized by target cells

Figure 1. ExoGlow-Protein enables clear visualization of labeled EVs being internalized by target cells. We labeled HEK293T EVs with ExoGlow-Protein and followed uptake by HEK293T cells. The punctate fluorescence signal shows internalization of labeled EVs by the target cells.

Punctate staining pattern is EV-specific

Figure 2. Punctate staining pattern is EV-specific. The punctate staining pattern seen in Figure 1 and Panel A is due to ExoGlow-Protein-labeled EVs. When ExoGlow-Protein is added directly to target HEK203T cells (Panel B), only diffuse, non-specific staining is seen.

ExoGlow-Protein signal is stable for up to at least 7 days after administration of labeled EVs to cells

Figure 3. ExoGlow-Protein signal is stable for up to at least 7 days after administration of labeled EVs to cells.

FAQs

Resources

Citations

  • Nocera, AL, et al. (2018) Exosome swarms eliminate airway pathogens and provide passive epithelial immunoprotection through nitric oxide. J. Allergy Clin. Immunol.. 2018;. PM ID: 30442371

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOGP400A-1 ExoGlowTM-Protein EV Labeling Kit (Blue) 20 Reactions $340
- +

Overview

Overview

See EVsbetter with reagents specifically optimized for labeling

SBI’s new generation of extracellular vesicle (EV) labeling reagents take your EV visualization to new levels of clarity, with low background and high selectivity. Unlike general-purpose labeling reagents that are not optimized for EVs and suffer from high levels of background signal, ExoGlow-Protein Blue improves your ability to track and localize EVs by specifically and covalently labeling internal EV proteins with a proprietary blue fluorescent dye that delivers very low levels of background signal. The result is unmatched EV imaging for more accurate studies (see below for our full range of next generation ExoGlow reagents).

  • Specific—carefully developed to generate a robust signal specific for internal EV proteins, leading to very low levels of background
  • Compatible—delivers robust performance on EVs isolated using all methods tested—including ExoQuick, ultracentrifugation, and column-based workflows
  • Easy-to-use—labeling protocol is quick and straightforward
  • Powerful—can be used with as little as 200 µg of EVs
ExoGlow-Protein Blue properties:
Excitation: 403 nm
Emission: 454 nm
Laser line: 405 nm
Available next generation ExoGlow EV imaging reagents
EV Component LabeledCat. #ProductExcitation (nm)Emission (nm)Laser Line (nm)
Internal proteinEXOGP100A-1ExoGlow-Protein EV Labeling Kit (Red)573
588561
EXOGP300A-1ExoGlow-Protein EV Labeling Kit (Green)

511525488
EXOGP400A-1ExoGlow-Protein EV Labeling Kit (Blue)403454
405
RNAEXOGR800A-1ExoGlow-RNA EV Labeling Kit485537488
MembraneEXOGM600A-1ExoGlow-Membrane EV Labeling Kit465635488

How It Works

Supporting Data

Supporting Data

Imaging EVs with a new level of clarity

ExoGlow-Protein enables clear visualization of labeled EVs being internalized by target cells

Figure 1. ExoGlow-Protein enables clear visualization of labeled EVs being internalized by target cells. We labeled HEK293T EVs with ExoGlow-Protein and followed uptake by HEK293T cells. The punctate fluorescence signal shows internalization of labeled EVs by the target cells.

Punctate staining pattern is EV-specific

Figure 2. Punctate staining pattern is EV-specific. The punctate staining pattern seen in Figure 1 and Panel A is due to ExoGlow-Protein-labeled EVs. When ExoGlow-Protein is added directly to target HEK203T cells (Panel B), only diffuse, non-specific staining is seen.

ExoGlow-Protein signal is stable for up to at least 7 days after administration of labeled EVs to cells

Figure 3. ExoGlow-Protein signal is stable for up to at least 7 days after administration of labeled EVs to cells.

FAQs

Citations

  • Nocera, AL, et al. (2018) Exosome swarms eliminate airway pathogens and provide passive epithelial immunoprotection through nitric oxide. J. Allergy Clin. Immunol.. 2018;. PM ID: 30442371