pPACKH1 HIV Lentivector Packaging Kit

Generate high titers of transduction-ready pseudoviral particles with this HIV-based Lentivector Packaging Kit
  • Effectively leverage the powerful lentiviral transduction platform with SBI’s optimized HIV-based and FIV-based packaging systems
  • Easily generate high-titer lentiviral preparations
  • Efficiently deliver most third-generation lentivector expression constructs
  • Successfully reach: A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles A broad range of cell types including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more
  • A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles

Products

Catalog Number Description Size Price Quantity Add to Cart
LV500A-1 pPACKH1 HIV Lentivector Packaging Kit 10 Transfections $508.00
- +
Contact Us
LV510A-1 pPACKH1-XL HIV Lentivector Packaging Kit 25 Transfections $1,119.00
- +
Contact Us

Overview

Overview

Produce high-titer lentivirus with this HIV-based Lentivector Packaging Kit Designed to efficiently package most third-generation lentivectors, the pPACKH1 HIV Lentivector Packaging Kit makes it easy to deliver and express lentiviral constructs in a wide-range of mammalian cells.
  • Effectively leverage the powerful lentiviral transduction platform with SBI’s optimized HIV-based and FIV-based packaging systems
  • Easily generate high-titer lentiviral preparations
  • Efficiently deliver most third-generation lentivector expression constructs
  • Successfully reach:
    • A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles
    • A broad range of cell types including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more
The pPACKH1 Packaging Plasmid Mix consists of three plasmids that produce all the structural and replication proteins needed to transcribe and package an RNA copy of the expression lentivector into recombinant, VSV-G-pseudotyped lentiviral particles. Just co-transfect the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells, isolate virus particles, and transduce into target cells. Upon transduction, the lentivector construct becomes stably integrated into the genome of the target cells for long-term expression of genes, miRNAs, shRNAs, etc.

How It Works

How It Works

The lentivirus packaging, production, and transduction workflow

Use the pPACKH1 HIV Lentivector Packaging Kit to package your lentivector expression construct into high-titer lentivirus particle preparations

Supporting Data

Supporting Data

SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism

SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism

Resources

Citations

  • Zhao, H, et al. (2021) Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p. Theranostics. 1970 Jan 1; 11(15):7600-7615. PM ID: 34158869
  • He, Q, et al. (2021) Cancer-secreted exosomal miR-21-5p induces angiogenesis and vascular permeability by targeting KRIT1. Cell death & disease. 1970 Jan 1; 12(6):576. PM ID: 34088891
  • Luo, M, et al. (2021) PD0325901, an ERK inhibitor, enhances the efficacy of PD-1 inhibitor in non-small cell lung carcinoma. Acta Pharmaceutica Sinica B. 1970 Jan 1;. Link: Acta Pharmaceutica Sinica B
  • Golubovskaya, V, et al. (2021) Novel CD37, Humanized CD37 and Bi-Specific Humanized CD37-CD19 CAR-T Cells Specifically Target Lymphoma. Cancers. 1970 Jan 1; 13(5). PM ID: 33652767
  • Clark, B. (2021) ANTI-GLUTEN SYNTHETIC NOTCH RECEPTOR T CELLS FOR THE TREATMENT OF CELIAC DISEASE. Thesis. 1970 Jan 1;. Link: Thesis
  • Rong, QX, et al. (2021) GM-CSF mediates immune evasion via upregulation of PD-L1 expression in extranodal natural killer/T cell lymphoma. Molecular cancer. 1970 Jan 1; 20(1):80. PM ID: 34051805
  • Luo, J, et al. (2021) lncRNA GAS6-AS1 inhibits progression and glucose metabolism reprogramming in LUAD via repressing E2F1-mediated transcription of GLUT1. Molecular Therapy - Nucleic Acids. 1970 Jan 1; 25:11-24. Link: Molecular Therapy - Nucleic Acids
  • Slater, BT, et al. (2020) Structural insight into T cell coinhibition by PD-1H (VISTA). Proc. Natl. Acad. Sci. U.S.A.. 1970 Jan 1; 117(3):1648-1657. PM ID: 31919279
  • Tanaka, M, et al. (2020) Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2. Monoclon Antib Immunodiagn Immunother. 1970 Jan 1;. PM ID: 31916900
  • Liang, G, et al. (2020) miR-196b-5p-mediated downregulation of TSPAN12 and GATA6 promotes tumor progression in non-small cell lung cancer. Proc. Natl. Acad. Sci. U.S.A.. 1970 Jan 1; 117(8):4347-4357. PM ID: 32041891
  • Hu, Y, et al. (2020) Targeting of CD38 by the tumor suppressor miR-26a serves as a novel potential therapeutic agent in multiple myeloma. Cancer Res.. 1970 Jan 1;. PM ID: 32193289
  • Mali, RS, et al. (2020) Venetoclax combines synergistically with FLT3 inhibition to effectively target leukemic cells in FLT3-ITD+ acute myeloid leukemia models. Haematologica. 1970 Jan 1;. PM ID: 32414851
  • Huang, Y, et al. (2020) STIM1 controls calcineurin/Akt/mTOR/NFATC2‑mediated osteoclastogenesis induced by RANKL/M‑CSF. Exp Ther Med. 1970 Jan 1;. Link: Exp Ther Med
  • Knarr, M, et al. (2020) miR-181a initiates and perpetuates oncogenic transformation through the regulation of innate immune signaling. Nat Commun. 1970 Jan 1; 11(1):3231. PM ID: 32591511
  • Geng, D, et al. (2020) Induction of IRAK-M in melanoma induces caspase-3 dependent apoptosis by reducing TRAF6 and calpastatin levels. Commun Biol. 1970 Jan 1; 3(1):306. PM ID: 32533049
  • Gao, W, et al. (2020) NADPH oxidase 5α promotes the formation of CD271 tumor-initiating cells in oral cancer. Am J Cancer Res. 1970 Jan 1; 10(6):1710-1727. PM ID: 32642285
  • Wang, Z, et al. (2020) T95 Nucleophosmin Phosphorylation as a Novel Mediator and Marker of Regulated Cell Death in Acute Kidney Injury. Am. J. Physiol. Renal Physiol.. 1970 Jan 1;. PM ID: 32686519
  • Luo, M, et al. (2020) Mitomycin C enhanced the efficacy of PD-L1 blockade in non-small cell lung cancer. Signal Transduct Target Ther. 1970 Jan 1; 5(1):141. PM ID: 32855386
  • Duan, H, et al. (2020) Plasminogen regulates mesenchymal stem cell-mediated tissue repair after ischemia through Cyr61 activation. JCI Insight. 1970 Jan 1; 5(15). PM ID: 32759492
  • Wang, T, et al. (2020) COL4A1 promotes the growth and metastasis of hepatocellular carcinoma cells by activating FAK-Src signaling. J. Exp. Clin. Cancer Res.. 1970 Jan 1; 39(1):148. PM ID: 32746865

Products

Catalog Number Description Size Price Quantity Add to Cart
LV500A-1 pPACKH1 HIV Lentivector Packaging Kit 10 Transfections $508.00
- +
Contact Us
LV510A-1 pPACKH1-XL HIV Lentivector Packaging Kit 25 Transfections $1,119.00
- +
Contact Us

Overview

Overview

Produce high-titer lentivirus with this HIV-based Lentivector Packaging Kit Designed to efficiently package most third-generation lentivectors, the pPACKH1 HIV Lentivector Packaging Kit makes it easy to deliver and express lentiviral constructs in a wide-range of mammalian cells.
  • Effectively leverage the powerful lentiviral transduction platform with SBI’s optimized HIV-based and FIV-based packaging systems
  • Easily generate high-titer lentiviral preparations
  • Efficiently deliver most third-generation lentivector expression constructs
  • Successfully reach:
    • A wide variety of mammalian cells with VSV-G pseudotyped lentiviral particles
    • A broad range of cell types including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more
The pPACKH1 Packaging Plasmid Mix consists of three plasmids that produce all the structural and replication proteins needed to transcribe and package an RNA copy of the expression lentivector into recombinant, VSV-G-pseudotyped lentiviral particles. Just co-transfect the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells, isolate virus particles, and transduce into target cells. Upon transduction, the lentivector construct becomes stably integrated into the genome of the target cells for long-term expression of genes, miRNAs, shRNAs, etc.

How It Works

How It Works

The lentivirus packaging, production, and transduction workflow

Use the pPACKH1 HIV Lentivector Packaging Kit to package your lentivector expression construct into high-titer lentivirus particle preparations

Supporting Data

Supporting Data

SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism

SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism

Citations

  • Zhao, H, et al. (2021) Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p. Theranostics. 1970 Jan 1; 11(15):7600-7615. PM ID: 34158869
  • He, Q, et al. (2021) Cancer-secreted exosomal miR-21-5p induces angiogenesis and vascular permeability by targeting KRIT1. Cell death & disease. 1970 Jan 1; 12(6):576. PM ID: 34088891
  • Luo, M, et al. (2021) PD0325901, an ERK inhibitor, enhances the efficacy of PD-1 inhibitor in non-small cell lung carcinoma. Acta Pharmaceutica Sinica B. 1970 Jan 1;. Link: Acta Pharmaceutica Sinica B
  • Golubovskaya, V, et al. (2021) Novel CD37, Humanized CD37 and Bi-Specific Humanized CD37-CD19 CAR-T Cells Specifically Target Lymphoma. Cancers. 1970 Jan 1; 13(5). PM ID: 33652767
  • Clark, B. (2021) ANTI-GLUTEN SYNTHETIC NOTCH RECEPTOR T CELLS FOR THE TREATMENT OF CELIAC DISEASE. Thesis. 1970 Jan 1;. Link: Thesis
  • Rong, QX, et al. (2021) GM-CSF mediates immune evasion via upregulation of PD-L1 expression in extranodal natural killer/T cell lymphoma. Molecular cancer. 1970 Jan 1; 20(1):80. PM ID: 34051805
  • Luo, J, et al. (2021) lncRNA GAS6-AS1 inhibits progression and glucose metabolism reprogramming in LUAD via repressing E2F1-mediated transcription of GLUT1. Molecular Therapy - Nucleic Acids. 1970 Jan 1; 25:11-24. Link: Molecular Therapy - Nucleic Acids
  • Slater, BT, et al. (2020) Structural insight into T cell coinhibition by PD-1H (VISTA). Proc. Natl. Acad. Sci. U.S.A.. 1970 Jan 1; 117(3):1648-1657. PM ID: 31919279
  • Tanaka, M, et al. (2020) Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2. Monoclon Antib Immunodiagn Immunother. 1970 Jan 1;. PM ID: 31916900
  • Liang, G, et al. (2020) miR-196b-5p-mediated downregulation of TSPAN12 and GATA6 promotes tumor progression in non-small cell lung cancer. Proc. Natl. Acad. Sci. U.S.A.. 1970 Jan 1; 117(8):4347-4357. PM ID: 32041891
  • Hu, Y, et al. (2020) Targeting of CD38 by the tumor suppressor miR-26a serves as a novel potential therapeutic agent in multiple myeloma. Cancer Res.. 1970 Jan 1;. PM ID: 32193289
  • Mali, RS, et al. (2020) Venetoclax combines synergistically with FLT3 inhibition to effectively target leukemic cells in FLT3-ITD+ acute myeloid leukemia models. Haematologica. 1970 Jan 1;. PM ID: 32414851
  • Huang, Y, et al. (2020) STIM1 controls calcineurin/Akt/mTOR/NFATC2‑mediated osteoclastogenesis induced by RANKL/M‑CSF. Exp Ther Med. 1970 Jan 1;. Link: Exp Ther Med
  • Knarr, M, et al. (2020) miR-181a initiates and perpetuates oncogenic transformation through the regulation of innate immune signaling. Nat Commun. 1970 Jan 1; 11(1):3231. PM ID: 32591511
  • Geng, D, et al. (2020) Induction of IRAK-M in melanoma induces caspase-3 dependent apoptosis by reducing TRAF6 and calpastatin levels. Commun Biol. 1970 Jan 1; 3(1):306. PM ID: 32533049
  • Gao, W, et al. (2020) NADPH oxidase 5α promotes the formation of CD271 tumor-initiating cells in oral cancer. Am J Cancer Res. 1970 Jan 1; 10(6):1710-1727. PM ID: 32642285
  • Wang, Z, et al. (2020) T95 Nucleophosmin Phosphorylation as a Novel Mediator and Marker of Regulated Cell Death in Acute Kidney Injury. Am. J. Physiol. Renal Physiol.. 1970 Jan 1;. PM ID: 32686519
  • Luo, M, et al. (2020) Mitomycin C enhanced the efficacy of PD-L1 blockade in non-small cell lung cancer. Signal Transduct Target Ther. 1970 Jan 1; 5(1):141. PM ID: 32855386
  • Duan, H, et al. (2020) Plasminogen regulates mesenchymal stem cell-mediated tissue repair after ischemia through Cyr61 activation. JCI Insight. 1970 Jan 1; 5(15). PM ID: 32759492
  • Wang, T, et al. (2020) COL4A1 promotes the growth and metastasis of hepatocellular carcinoma cells by activating FAK-Src signaling. J. Exp. Clin. Cancer Res.. 1970 Jan 1; 39(1):148. PM ID: 32746865