pPACK-SPIKE™ D614G, SARS-CoV-2 “S” Pseudotype - D614G Mutant - Lentivector Packaging Mix

Study the SARS-CoV-2 D614G Spike protein mutant with this system for generating high titers of D614G Spike protein pseudotyped lentivirus particles.
  • Based on SBI’s popular and highly cited pPACKH1 Packaging System
  • Uses codon-optimized SARS-CoV-2 D614G “S” protein in place of VSV-G envelope protein
  • Ideal for COVID-19 research, such as neutralization assays, under BSL2 conditions
  • Package any 3rd-generation lentivector reporter of your choice, including SBI’s popular LentiLabeler reporters
  • Use with pPACK-BALD™, an envelope protein-free lentivector packaging system that is an ideal negative control
  • Compare to the Spike protein from the original Wuhan-Hu-1 strain when you use pPACK-SPIKE

Products

Catalog Number Description Size Price Quantity Add to Cart
CVD19-530A-1 pPACK-SPIKE D614G, SARS-CoV-2 “S” Pseudotype – D614G Mutant – Lentivector Packaging Mix 10 Reactions $650.00
- +
Contact Us
CVD19-540A-1 pPACK-SPIKE D614G, SARS-CoV-2 “S” Pseudotype – D614G Mutant – Lentivector Packaging Mix (XL) 25 Reactions $1,295.00
- +
Contact Us
CVD19-550A-KIT pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) 1 Kit $1,095.00
- +
Contact Us

Overview

Overview

Safely study SARS-CoV-2 interactions  Designed to efficiently package most third-generation lentivectors, the pPACK-SPIKE D614G SARS-CoV-2 “S” Pseudotype - D614G Mutant - Lentivector Packaging Mix speeds and simplifies the preparation of lentiviral particles pseudotyped with the SARS-CoV-2 D614G Spike glycoprotein. Based on SBI's highly cited pPACKH1 packaging system but with a truncated SARS-CoV-2 D614G Spike protein [1] replacing the standard VSV-G envelope protein, the pPACK-SPIKE D614G Packaging Mix consists of three plasmids that produce all of the structural and  replication proteins needed to transcribe and package an RNA copy of an expression lentivector into recombinant, D614G “Spike” pseudotyped lentiviral particles. As a result, you can conduct a range of SARS-CoV-2 studies under BSL-2 conditions, including neutralization assays, studies of virus interactions with host surface proteins, and the development of vaccines and therapeutics.

For added convenience we also offer pPACK-BALD™, an envelope protein-free lentivector packaging mix that can be used as a negative control for pPACK-SPIKE D614G studies, or for creating lentivirus particles pseudotyped with the envelope protein of your choice. In addition, you can compare the D614G Spike protein to the Spike protein from the original Wuhan-Hu-1 strain with pPACK-SPIKE.
  • Based on SBI’s popular and highly cited pPACKH1 Packaging System
  • Uses codon-optimized SARS-CoV-2 D614G “S” protein in place of VSV-G envelope protein
  • Efficiently incorporates the D614G Spike protein into pseudoviral particles due to a 19 aa C-terminal truncation [1]
  • Ideal for COVID-19 research, such as neutralization assays, under BSL2 conditions
  • Package any 3rd-generation lentivector reporter of your choice, including SBI’s popular LentiLabeler reporters
  • Available as a packaging mix or bundled into a convenient kit that includes PureFection™ Transfection Reagent and PEG-it Virus Precipitation Solution
  • Use with pPACK-BALD™, an envelope protein-free lentivector packaging system that is an ideal negative control
  • Compare to the Spike protein from the original Wuhan-Hu-1 strain when you use pPACK-SPIKE
pPACK-SPIKE D614G SARS-CoV-2 “S” Pseudotype -D614G Mutant - Lentivector Packaging Mix is available as stand-alone packaging mixes with sufficient plasmids for 10 reactions (standard size) or 25 reactions (XL), as well as in a convenient kit format that includes PureFection™ Transfection Reagent and PEG-it Virus Precipitation Solution.
See all the available pPACK-SPIKE S protein variants:

Catalog NumberProduct DescriptionSpike protein variant
CVD19-500A-1pPACK-SPIKE SARS-CoV-2 “S” Pseudotype Lentivector Packaging MixOriginal Wuhan-Hu-1 strain
CVD19-590A-1pPACK-SPIKE Alpha (B.1.1.7), SARS-CoV-2 "S" Pseudotype - Alpha (B.1.1.7, UK) Variant - Lentivector Packaging MixΔH69/V70, ΔY144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
CVD19-640A-1pPACK-SPIKE Beta (B.1.351), SARS-CoV-2 "S" Pseudotype - Beta (B.1.351) Variant - Lentivector Packaging MixL18F, D80A, D215G, Δ242-244, R246I, K417N, E484K, N501Y, D614G, A701V
CVD19-650A-1pPACK-SPIKE Delta (B.1.617.2), SARS-CoV-2 "S" Pseudotype - Delta (B.1.617.2) Variant - Lentivector Packaging MixT19R, G142D, ΔE156-157, R158G, L452R, T478K, D614G, P681R, D950N
CVD19-630A-1pPACK-SPIKE Gamma (P1), SARS-CoV-2 "S" Pseudotype - Gamma (P1) Variant - Lentivector Packaging MixL18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F
CVD19-530A-1pPACK-SPIKE D614G, SARS-CoV-2 “S” Pseudotype – D614G Mutant – Lentivector Packaging MixD614G
CVD19-560A-1pPACK-SPIKE N501Y, SARS-CoV-2 "S" Pseudotype - N501Y Mutant - Lentivector Packaging MixN501Y
CVD19-610A-1pPACK-SPIKE B.1.429, SARS-CoV-2 "S" Pseudotype - B.1.429 (CAL.20C) Variant - Lentivector Packaging MixS13I, W152C, L452R, & D614G
CVD19-620A-1pPACK-SPIKE B.1.525, SARS-CoV-2 "S" Pseudotype - B.1.525 Variant - Lentivector Packaging MixΔH69/V70, Q52R, E484K, D614G, Q677H, & F888L
CVD19-580A-1pPACK-SPIKE B.1.351 RBD Mutations Lentivector Packaging MixK417N, E484K, & N501Y
CVD19-600A-1pPACK-SPIKE B.1.351 S1 Mutations (RBD+D614G) Lentivector Packaging MixK417N, E484K, N501Y, & D614G
LV550A-1pPACK-BALD™ (Negative Control Packaging Mix)An envelope protein-free negative control for pPACK-SPIKE
References
  1. Ou X, et al. Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat. commun. 2020 Mar 27;11(1):1620. doi: 10.1038/s41467-020-15562-9. PMCID: PMC7100515.

How It Works

How It Works

pPACK-SPIKE is easy to use—simply co-transfect the pPACK-SPIKE Plasmids and your lentivector construct into 293TN producer cells or other lentiviral production cells, isolate pseudoviral particles with an optional concentration step, and conduct your studies.

Supporting Data

Supporting Data

To see data generated using the pPACK-SPIKE with the S protein from the original Wuhan-Hu-1 strain, visit the pPACK-SPIKE  page.

Resources

Citations

We're sorry, an error has occurred while generating this content.
pPACK-SPIKE™ D614G, SARS-CoV-2 “S” Pseudotype - D614G Mutant - Lentivector Packaging Mix $650.00

Products

Catalog Number Description Size Price Quantity Add to Cart
CVD19-530A-1 pPACK-SPIKE D614G, SARS-CoV-2 “S” Pseudotype – D614G Mutant – Lentivector Packaging Mix 10 Reactions $650.00
- +
Contact Us
CVD19-540A-1 pPACK-SPIKE D614G, SARS-CoV-2 “S” Pseudotype – D614G Mutant – Lentivector Packaging Mix (XL) 25 Reactions $1,295.00
- +
Contact Us
CVD19-550A-KIT pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) 1 Kit $1,095.00
- +
Contact Us

Overview

Overview

Safely study SARS-CoV-2 interactions  Designed to efficiently package most third-generation lentivectors, the pPACK-SPIKE D614G SARS-CoV-2 “S” Pseudotype - D614G Mutant - Lentivector Packaging Mix speeds and simplifies the preparation of lentiviral particles pseudotyped with the SARS-CoV-2 D614G Spike glycoprotein. Based on SBI's highly cited pPACKH1 packaging system but with a truncated SARS-CoV-2 D614G Spike protein [1] replacing the standard VSV-G envelope protein, the pPACK-SPIKE D614G Packaging Mix consists of three plasmids that produce all of the structural and  replication proteins needed to transcribe and package an RNA copy of an expression lentivector into recombinant, D614G “Spike” pseudotyped lentiviral particles. As a result, you can conduct a range of SARS-CoV-2 studies under BSL-2 conditions, including neutralization assays, studies of virus interactions with host surface proteins, and the development of vaccines and therapeutics.

For added convenience we also offer pPACK-BALD™, an envelope protein-free lentivector packaging mix that can be used as a negative control for pPACK-SPIKE D614G studies, or for creating lentivirus particles pseudotyped with the envelope protein of your choice. In addition, you can compare the D614G Spike protein to the Spike protein from the original Wuhan-Hu-1 strain with pPACK-SPIKE.
  • Based on SBI’s popular and highly cited pPACKH1 Packaging System
  • Uses codon-optimized SARS-CoV-2 D614G “S” protein in place of VSV-G envelope protein
  • Efficiently incorporates the D614G Spike protein into pseudoviral particles due to a 19 aa C-terminal truncation [1]
  • Ideal for COVID-19 research, such as neutralization assays, under BSL2 conditions
  • Package any 3rd-generation lentivector reporter of your choice, including SBI’s popular LentiLabeler reporters
  • Available as a packaging mix or bundled into a convenient kit that includes PureFection™ Transfection Reagent and PEG-it Virus Precipitation Solution
  • Use with pPACK-BALD™, an envelope protein-free lentivector packaging system that is an ideal negative control
  • Compare to the Spike protein from the original Wuhan-Hu-1 strain when you use pPACK-SPIKE
pPACK-SPIKE D614G SARS-CoV-2 “S” Pseudotype -D614G Mutant - Lentivector Packaging Mix is available as stand-alone packaging mixes with sufficient plasmids for 10 reactions (standard size) or 25 reactions (XL), as well as in a convenient kit format that includes PureFection™ Transfection Reagent and PEG-it Virus Precipitation Solution.
See all the available pPACK-SPIKE S protein variants:

Catalog NumberProduct DescriptionSpike protein variant
CVD19-500A-1pPACK-SPIKE SARS-CoV-2 “S” Pseudotype Lentivector Packaging MixOriginal Wuhan-Hu-1 strain
CVD19-590A-1pPACK-SPIKE Alpha (B.1.1.7), SARS-CoV-2 "S" Pseudotype - Alpha (B.1.1.7, UK) Variant - Lentivector Packaging MixΔH69/V70, ΔY144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
CVD19-640A-1pPACK-SPIKE Beta (B.1.351), SARS-CoV-2 "S" Pseudotype - Beta (B.1.351) Variant - Lentivector Packaging MixL18F, D80A, D215G, Δ242-244, R246I, K417N, E484K, N501Y, D614G, A701V
CVD19-650A-1pPACK-SPIKE Delta (B.1.617.2), SARS-CoV-2 "S" Pseudotype - Delta (B.1.617.2) Variant - Lentivector Packaging MixT19R, G142D, ΔE156-157, R158G, L452R, T478K, D614G, P681R, D950N
CVD19-630A-1pPACK-SPIKE Gamma (P1), SARS-CoV-2 "S" Pseudotype - Gamma (P1) Variant - Lentivector Packaging MixL18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F
CVD19-530A-1pPACK-SPIKE D614G, SARS-CoV-2 “S” Pseudotype – D614G Mutant – Lentivector Packaging MixD614G
CVD19-560A-1pPACK-SPIKE N501Y, SARS-CoV-2 "S" Pseudotype - N501Y Mutant - Lentivector Packaging MixN501Y
CVD19-610A-1pPACK-SPIKE B.1.429, SARS-CoV-2 "S" Pseudotype - B.1.429 (CAL.20C) Variant - Lentivector Packaging MixS13I, W152C, L452R, & D614G
CVD19-620A-1pPACK-SPIKE B.1.525, SARS-CoV-2 "S" Pseudotype - B.1.525 Variant - Lentivector Packaging MixΔH69/V70, Q52R, E484K, D614G, Q677H, & F888L
CVD19-580A-1pPACK-SPIKE B.1.351 RBD Mutations Lentivector Packaging MixK417N, E484K, & N501Y
CVD19-600A-1pPACK-SPIKE B.1.351 S1 Mutations (RBD+D614G) Lentivector Packaging MixK417N, E484K, N501Y, & D614G
LV550A-1pPACK-BALD™ (Negative Control Packaging Mix)An envelope protein-free negative control for pPACK-SPIKE
References
  1. Ou X, et al. Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat. commun. 2020 Mar 27;11(1):1620. doi: 10.1038/s41467-020-15562-9. PMCID: PMC7100515.

How It Works

How It Works

pPACK-SPIKE is easy to use—simply co-transfect the pPACK-SPIKE Plasmids and your lentivector construct into 293TN producer cells or other lentiviral production cells, isolate pseudoviral particles with an optional concentration step, and conduct your studies.

Supporting Data

Supporting Data

To see data generated using the pPACK-SPIKE with the S protein from the original Wuhan-Hu-1 strain, visit the pPACK-SPIKE  page.

Citations

We're sorry, an error has occurred while generating this content.