pGreenFire 2.0 AP-1 Reporter

Study oncogenic signaling with the next-gen pGreenFire 2.0 AP-1 Reporter (red firefly luciferase & GFP), engineered for reliable stable cell line generation.
  • NEW: Now also available as a stable cell line!
  • Sort responsive cells with GFP
  • Measure activity with red firefly luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create your own stable signaling pathway reporter cell lines of interest
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
  • Move faster to data collection with the ready-to-use reporter cell line

Products

Catalog Number Description Size Price Quantity Add to Cart
TR452PA-P pGreenFire 2.0 AP-1 reporter plasmid (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro) 10 µg $673.00
- +
Contact Us
TR452C-P pGreenFire 2.0 AP-1 clonal 293T reporter cell line (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro) 2 x 10^6 Cells $2,955.00
- +
Contact Us
TR452VA-P pGreenFire 2.0 AP-1 reporter virus (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro) >2 x 10^6 IFUs $673.00
- +
Contact Us

Overview

Overview

Monitor oncogenic signaling in real time with our re-engineered pGreenFire 2.0 Lentivectors SBI has upgraded our popular pGreenFire signaling pathway reporter lentivectors with a design that leads to more reliable generation of stable cell lines. We’ve also swapped in the red firefly luciferase reporter (rFLuc), which opens up the possibility of performing a dual-spectral luciferase assay and delivers greater sensitivity for in vivo applications than conventional luciferase.
NOW AVAILABLE AS A READY-TO-USE STABLE CELL LINE
With the pGreenFire 2.0 AP-1 Reporter Lentivector, Virus, and clonally-derived Stable Cell Line (pGF2-AP-1-rFluc-T2A-GFP-mPGK-Puro), the core reporter functionality is similar to the original pGreenFire lentivector—AP-1 transcriptional response elements (TREs) are placed upstream of a minimal CMV promoter (mCMV) which together drive co-expression of rFLuc and GFP in response to AP-1 activity. The result is the ability to quantitatively measure AP-1 activity using both fluorescence and luciferase activity. What makes our next-gen pGreenFire 2.0 vectors even better than other TRE reporter vectors is the smart design, which adds in a constitutive selection cassette for stable cell line generation while minimizing interference with the upstream TRE. By using a weak/moderate mPGK promoter to drive the antibiotic selection marker (puromycin resistance) and carefully arranging the conditional reporter genes, the selection marker is reliably expressed without compromising conditional expression of rFLuc and GFP. As with our original pGreenFire1 vectors, all pGreenFire 2.0 lentivectors leverage our reliable lentivector technology and save you time with pre-built signal transduction pathway reporters that come as ready-to-transduce pre-packaged lentivirus and plasmid that can be transfected into the lentivirus producing system of your choice*.
  • Sort responsive cells with GFP
  • Measure activity with red firefly luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGreenFire 2.0 AP-1 Reporter Lentivector & Virus *Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our pGreenFire 2.0 transcriptional response element reporters in action The pGreenFire 2.0 AP-1 Reporter efficiently and quantitatively reports on AP-1 activity in 293FT cells Figure 1. The pGreenFire 2.0 AP-1 Lentivector efficiently and quantitatively reports on AP-1 activity in 293T cells. Relative luciferase activity (A) and GFP activity (B) both increase in response to PMA, an AP-1inducer. Induction is specific to the AP-1 transcription response elements as the pGreenFire 2.0 mCMV Negative Control displays very little luciferase activity regardless of the presence of inducer.
Figure 2. The pGreenFire 2.0 AP-1 reporter cassette efficiently and quantitatively reports on AP-1 activity in the pGreenFire 2.0 AP-1 clonal 293T reporter cell line. Luciferase activity (A) and GFP intensity (B) both increase in response to PMA.

Resources

We're sorry, an error has occurred while generating this content.

Citations

We're sorry, an error has occurred while generating this content.

Products

Catalog Number Description Size Price Quantity Add to Cart
TR452PA-P pGreenFire 2.0 AP-1 reporter plasmid (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro) 10 µg $673.00
- +
Contact Us
TR452C-P pGreenFire 2.0 AP-1 clonal 293T reporter cell line (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro) 2 x 10^6 Cells $2,955.00
- +
Contact Us
TR452VA-P pGreenFire 2.0 AP-1 reporter virus (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro) >2 x 10^6 IFUs $673.00
- +
Contact Us

Overview

Overview

Monitor oncogenic signaling in real time with our re-engineered pGreenFire 2.0 Lentivectors SBI has upgraded our popular pGreenFire signaling pathway reporter lentivectors with a design that leads to more reliable generation of stable cell lines. We’ve also swapped in the red firefly luciferase reporter (rFLuc), which opens up the possibility of performing a dual-spectral luciferase assay and delivers greater sensitivity for in vivo applications than conventional luciferase.
NOW AVAILABLE AS A READY-TO-USE STABLE CELL LINE
With the pGreenFire 2.0 AP-1 Reporter Lentivector, Virus, and clonally-derived Stable Cell Line (pGF2-AP-1-rFluc-T2A-GFP-mPGK-Puro), the core reporter functionality is similar to the original pGreenFire lentivector—AP-1 transcriptional response elements (TREs) are placed upstream of a minimal CMV promoter (mCMV) which together drive co-expression of rFLuc and GFP in response to AP-1 activity. The result is the ability to quantitatively measure AP-1 activity using both fluorescence and luciferase activity. What makes our next-gen pGreenFire 2.0 vectors even better than other TRE reporter vectors is the smart design, which adds in a constitutive selection cassette for stable cell line generation while minimizing interference with the upstream TRE. By using a weak/moderate mPGK promoter to drive the antibiotic selection marker (puromycin resistance) and carefully arranging the conditional reporter genes, the selection marker is reliably expressed without compromising conditional expression of rFLuc and GFP. As with our original pGreenFire1 vectors, all pGreenFire 2.0 lentivectors leverage our reliable lentivector technology and save you time with pre-built signal transduction pathway reporters that come as ready-to-transduce pre-packaged lentivirus and plasmid that can be transfected into the lentivirus producing system of your choice*.
  • Sort responsive cells with GFP
  • Measure activity with red firefly luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
pGreenFire 2.0 AP-1 Reporter Lentivector & Virus *Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

How It Works

Supporting Data

Supporting Data

See our pGreenFire 2.0 transcriptional response element reporters in action The pGreenFire 2.0 AP-1 Reporter efficiently and quantitatively reports on AP-1 activity in 293FT cells Figure 1. The pGreenFire 2.0 AP-1 Lentivector efficiently and quantitatively reports on AP-1 activity in 293T cells. Relative luciferase activity (A) and GFP activity (B) both increase in response to PMA, an AP-1inducer. Induction is specific to the AP-1 transcription response elements as the pGreenFire 2.0 mCMV Negative Control displays very little luciferase activity regardless of the presence of inducer.
Figure 2. The pGreenFire 2.0 AP-1 reporter cassette efficiently and quantitatively reports on AP-1 activity in the pGreenFire 2.0 AP-1 clonal 293T reporter cell line. Luciferase activity (A) and GFP intensity (B) both increase in response to PMA.

Resources

We're sorry, an error has occurred while generating this content.

Citations

We're sorry, an error has occurred while generating this content.