PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector
Combining easy PiggyBac transgenesis with our robust and titratable cumate-inducible expression system, this PiggyBac Vector is designed for cDNA expression
- Make transgenic cell lines with a single transfection
- Integrate multiple PiggyBac Vectors in a single transfection
- Insert an expression cassette into human, mouse, and rat cells
- Deliver virtually any-sized DNA insert, from 10 – 100 kb
- Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| PBQM812A-1 | PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector | 10 µg | $1003 |
|
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Overview
Overview
Robust, titratable gene expression delivered using the PiggyBac Transposon System
More than just easy, consistent transgenesis with PiggyBac, the PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector (Cat.# PBQM812A-1) adds in the robust and titratable gene expression control of SBI’s cumate-inducible expression system. Clone your gene-of-interest into the MCS for cumate-inducible expression, which you can quantitatively monitor with the co-expressed GFP. This vector also co-expresses the cumate repressor, CymR, and puromycin resistance from the EF1α promoter. 
With the PiggyBac Transposon System, you can:
- Make transgenic cell lines with a single transfection
- Integrate multiple PiggyBac Vectors in a single transfection
- Insert an expression cassette into human, mouse, and rat cells
- Deliver virtually any-sized DNA insert, from 10 – 100 kb
- Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
- Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)
Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology.
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.
How It Works
How It Works
The PiggyBac Transposon System’s Cut-and-Paste Mechanism
The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.
Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.
- The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
- The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
- The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal
Tightly-controlled, inducible gene expression
Get robust, titratable gene expression with low background using SBI’s cumate-inducible vectors. These vectors take advantage of CymR, a repressor that binds to cumate operator sequences (CuO) with high affinity in the absence of cumate, a non-toxic small molecule. Providing much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.
- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Powerful—suitable for in vivo applications
Supporting Data
Supporting Data
Tight expression control with low background
Figure 2. In the absence of cumate, the cumate-inducible PiggyBac Vector shows undetectable levels of expression.
Figure 3. The PiggyBac cumate switch is titratable and can be turned off.
Resources
Citations
-
Uchino, S, et al. (2022) Live imaging of transcription sites using an elongating RNA polymerase II-specific probe. The Journal of cell biology. 1970 Jan 1; 221(2). PM ID: 34854870
-
Teixeira, A, et al. (2022) CelloSelect – A synthetic cellobiose metabolic pathway for selection of stable transgenic CHO cell lines. Metabolic Engineering. 1970 Jan 1; 70:23-30. Link: Metabolic Engineering
-
Rui, Y, et al. (2022) High-throughput and high-content bioassay enables tuning of polyester nanoparticles for cellular uptake, endosomal escape, and systemic in vivo delivery of mRNA. Science advances. 1970 Jan 1; 8(1):eabk2855. PM ID: 34985952
-
Vásquez-Limeta, A, et al. (2022) CPAP insufficiency leads to incomplete centrioles that duplicate but fragment. The Journal of cell biology. 1970 Jan 1; 221(5). PM ID: 35404385
-
Su, CJ, et al. (2022) Ligand-receptor promiscuity enables cellular addressing. Cell systems. 1970 Jan 1;. PM ID: 35421362
-
Klumpe, HE, et al. (2022) The context-dependent, combinatorial logic of BMP signaling. Cell systems. 1970 Jan 1;. PM ID: 35421361
-
Kitano, H, Kawabe, Y & Kamihira, M. (2022) HepG2-Based Designer Cells with Heat-Inducible Enhanced Liver Functions. Cells. 1970 Jan 1; 11(7). PM ID: 35406758
-
Sugiman-Marangos, SN, et al. (2022) Structures of distant diphtheria toxin homologs reveal functional determinants of an evolutionarily conserved toxin scaffold. Communications biology. 1970 Jan 1; 5(1):375. PM ID: 35440624
-
Ma, X, et al. (2022) Validation of reliable safe harbor locus for efficient porcine transgenesis. Functional & integrative genomics. 1970 Jan 1;. PM ID: 35412198
-
Nishimura, K, et al. (2022) Rapid conversion of human induced pluripotent stem cells into dopaminergic neurons by inducible expression of two transcription factors. Stem cells and development. 1970 Jan 1;. PM ID: 35420042
-
Liu, Z, Ramirez, A & Liu, X. (2022) Live Cell Imaging of Spatiotemporal Ca2+ Fluctuation Responses to Anticancer Drugs. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2488:227-236. PM ID: 35347692
-
Dandridge, S. (2022) Honors Thesis: Defining the effect of zinc on the proliferation of MDA-MB-231 cells compared to MCF10A cells. Thesis. 1970 Jan 1;. Link: Thesis
-
Yang, D, et al. (2022) Lineage tracing reveals the phylodynamics, plasticity, and paths of tumor evolution. Cell. 1970 Jan 1; 185(11):1905-1923.e25. PM ID: 35523183
-
Biswas, S, et al. (2022) Long-term hepatitis B virus infection of rhesus macaques requires suppression of host immunity. Nature communications. 1970 Jan 1; 13(1):2995. PM ID: 35637225
-
Breau, KA, et al. (2022) Efficient transgenesis and homology-directed gene targeting in monolayers of primary human small intestinal and colonic epithelial stem cells. Stem cell reports. 1970 Jan 1;. PM ID: 35523179
-
Lensch, S, et al. (2022) Dynamic spreading of chromatin-mediated gene silencing and reactivation between neighboring genes in single cells. eLife. 1970 Jan 1; 11. PM ID: 35678392
-
Gu, J, Sumer, H & Cromer, B. (2022) Efficient Generation of Stable Cell Lines with Inducible Neuronal Transgene Expression Using the piggyBac Transposon System. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2495:49-66. PM ID: 35696027
-
Wang, S, et al. (2021) Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion. Cell. 1970 Jan 1;. PM ID: 34133940
-
Ng, YH, et al. (2021) Efficient generation of dopaminergic induced neuronal cells with midbrain characteristics. Stem cell reports. 1970 Jan 1;. PM ID: 34171286
-
Ukaji, T, et al. (2021) Novel knock-in mouse model for the evaluation of the therapeutic efficacy and toxicity of human podoplanin-targeting agents. Cancer science. 1970 Jan 1; 112(6):2299-2313. PM ID: 33735501
- See More
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| PBQM812A-1 | PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector | 10 µg | $1003 |
|
||||
Overview
Overview
Robust, titratable gene expression delivered using the PiggyBac Transposon System
More than just easy, consistent transgenesis with PiggyBac, the PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector (Cat.# PBQM812A-1) adds in the robust and titratable gene expression control of SBI’s cumate-inducible expression system. Clone your gene-of-interest into the MCS for cumate-inducible expression, which you can quantitatively monitor with the co-expressed GFP. This vector also co-expresses the cumate repressor, CymR, and puromycin resistance from the EF1α promoter. With the PiggyBac Transposon System, you can:
- Make transgenic cell lines with a single transfection
- Integrate multiple PiggyBac Vectors in a single transfection
- Insert an expression cassette into human, mouse, and rat cells
- Deliver virtually any-sized DNA insert, from 10 – 100 kb
- Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
- Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)
Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology.
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.
How It Works
How It Works
The PiggyBac Transposon System’s Cut-and-Paste Mechanism
The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.
Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.
- The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
- The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
- The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal
Tightly-controlled, inducible gene expression
Get robust, titratable gene expression with low background using SBI’s cumate-inducible vectors. These vectors take advantage of CymR, a repressor that binds to cumate operator sequences (CuO) with high affinity in the absence of cumate, a non-toxic small molecule. Providing much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.
- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Powerful—suitable for in vivo applications
Supporting Data
Supporting Data
Tight expression control with low background
Figure 2. In the absence of cumate, the cumate-inducible PiggyBac Vector shows undetectable levels of expression.
Figure 3. The PiggyBac cumate switch is titratable and can be turned off.
Citations
-
Uchino, S, et al. (2022) Live imaging of transcription sites using an elongating RNA polymerase II-specific probe. The Journal of cell biology. 1970 Jan 1; 221(2). PM ID: 34854870
-
Teixeira, A, et al. (2022) CelloSelect – A synthetic cellobiose metabolic pathway for selection of stable transgenic CHO cell lines. Metabolic Engineering. 1970 Jan 1; 70:23-30. Link: Metabolic Engineering
-
Rui, Y, et al. (2022) High-throughput and high-content bioassay enables tuning of polyester nanoparticles for cellular uptake, endosomal escape, and systemic in vivo delivery of mRNA. Science advances. 1970 Jan 1; 8(1):eabk2855. PM ID: 34985952
-
Vásquez-Limeta, A, et al. (2022) CPAP insufficiency leads to incomplete centrioles that duplicate but fragment. The Journal of cell biology. 1970 Jan 1; 221(5). PM ID: 35404385
-
Su, CJ, et al. (2022) Ligand-receptor promiscuity enables cellular addressing. Cell systems. 1970 Jan 1;. PM ID: 35421362
-
Klumpe, HE, et al. (2022) The context-dependent, combinatorial logic of BMP signaling. Cell systems. 1970 Jan 1;. PM ID: 35421361
-
Kitano, H, Kawabe, Y & Kamihira, M. (2022) HepG2-Based Designer Cells with Heat-Inducible Enhanced Liver Functions. Cells. 1970 Jan 1; 11(7). PM ID: 35406758
-
Sugiman-Marangos, SN, et al. (2022) Structures of distant diphtheria toxin homologs reveal functional determinants of an evolutionarily conserved toxin scaffold. Communications biology. 1970 Jan 1; 5(1):375. PM ID: 35440624
-
Ma, X, et al. (2022) Validation of reliable safe harbor locus for efficient porcine transgenesis. Functional & integrative genomics. 1970 Jan 1;. PM ID: 35412198
-
Nishimura, K, et al. (2022) Rapid conversion of human induced pluripotent stem cells into dopaminergic neurons by inducible expression of two transcription factors. Stem cells and development. 1970 Jan 1;. PM ID: 35420042
-
Liu, Z, Ramirez, A & Liu, X. (2022) Live Cell Imaging of Spatiotemporal Ca2+ Fluctuation Responses to Anticancer Drugs. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2488:227-236. PM ID: 35347692
-
Dandridge, S. (2022) Honors Thesis: Defining the effect of zinc on the proliferation of MDA-MB-231 cells compared to MCF10A cells. Thesis. 1970 Jan 1;. Link: Thesis
-
Yang, D, et al. (2022) Lineage tracing reveals the phylodynamics, plasticity, and paths of tumor evolution. Cell. 1970 Jan 1; 185(11):1905-1923.e25. PM ID: 35523183
-
Biswas, S, et al. (2022) Long-term hepatitis B virus infection of rhesus macaques requires suppression of host immunity. Nature communications. 1970 Jan 1; 13(1):2995. PM ID: 35637225
-
Breau, KA, et al. (2022) Efficient transgenesis and homology-directed gene targeting in monolayers of primary human small intestinal and colonic epithelial stem cells. Stem cell reports. 1970 Jan 1;. PM ID: 35523179
-
Lensch, S, et al. (2022) Dynamic spreading of chromatin-mediated gene silencing and reactivation between neighboring genes in single cells. eLife. 1970 Jan 1; 11. PM ID: 35678392
-
Gu, J, Sumer, H & Cromer, B. (2022) Efficient Generation of Stable Cell Lines with Inducible Neuronal Transgene Expression Using the piggyBac Transposon System. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2495:49-66. PM ID: 35696027
-
Wang, S, et al. (2021) Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion. Cell. 1970 Jan 1;. PM ID: 34133940
-
Ng, YH, et al. (2021) Efficient generation of dopaminergic induced neuronal cells with midbrain characteristics. Stem cell reports. 1970 Jan 1;. PM ID: 34171286
-
Ukaji, T, et al. (2021) Novel knock-in mouse model for the evaluation of the therapeutic efficacy and toxicity of human podoplanin-targeting agents. Cancer science. 1970 Jan 1; 112(6):2299-2313. PM ID: 33735501
- See More