PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector

Combining easy PiggyBac transgenesis with our robust and titratable cumate-inducible expression system, this PiggyBac Vector is designed for cDNA expression

  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers

Products

Catalog Number Description Size Price Quantity Add to Cart
PBQM812A-1 PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector 10 µg $1061
- +

Overview

Overview

Robust, titratable gene expression delivered using the PiggyBac Transposon System

More than just easy, consistent transgenesis with PiggyBac, the PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector (Cat.# PBQM812A-1) adds in the robust and titratable gene expression control of SBI’s cumate-inducible expression system. Clone your gene-of-interest into the MCS for cumate-inducible expression, which you can quantitatively monitor with the co-expressed GFP. This vector also co-expresses the cumate repressor, CymR, and puromycin resistance from the EF1α promoter. PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector With the PiggyBac Transposon System, you can:

  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
  • Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)

Customer Agreements

Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to evaluate the technology.

For end user license information, see the following:

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.

How It Works

How It Works

The PiggyBac Transposon System’s Cut-and-Paste Mechanism

The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.

The PiggyBac Transposon System’s cut-and-paste mechanism

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.

  • The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
  • The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
  • The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal

Tightly-controlled, inducible gene expression

Get robust, titratable gene expression with low background using SBI’s cumate-inducible vectors. These vectors take advantage of CymR, a repressor that binds to cumate operator sequences (CuO) with high affinity in the absence of cumate, a non-toxic small molecule. Providing much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.How the cumate operator switch works

  • Robust—increase expression up to 32-fold
  • Adjustable—tune expression levels by titrating the amount of cumate
  • Reversible—turn expression on, then off, then on again
  • Powerful—suitable for in vivo applications

Supporting Data

Supporting Data

Tight expression control with low background

In the absence of cumate, the cumate-inducible PiggyBac Vector shows undetectable levels of expression

Figure 2. In the absence of cumate, the cumate-inducible PiggyBac Vector shows undetectable levels of expression.

The PiggyBac cumate switch is titratable and can be turned off

Figure 3. The PiggyBac cumate switch is titratable and can be turned off.

FAQs

Resources

Citations

  • Brouwer, I, de Kort, MAC & Lenstra, TL. (2024) Measuring Transcription Dynamics of Individual Genes Inside Living Cells. Methods in molecular biology (Clifton, N.J.). 2024; 2694:235-265. PM ID: 37824008
  • Matta, SK, et al. (2024) Genome-wide and targeted CRISPR screens identify RNF213 as a mediator of interferon gamma-dependent pathogen restriction in human cells. Proceedings of the National Academy of Sciences of the United States of America. 2024; 121(1):e2315865120. PM ID: 38147552
  • Hurt, RC, et al. (2023) Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria. Nature biotechnology. 2023;. PM ID: 36593411
  • Yamazaki, K, et al. (2023) Multivalent mannose-conjugated siRNA causes robust gene silencing in pancreatic macrophages in vivo. European Journal of Pharmaceutics and Biopharmaceutics. 2023;. Link: European Journal of Pharmaceutics and Biopharmaceutics
  • Shahin, WS, et al. (2023) Redox-dependent Igfbp2 signaling controls Brca1 DNA damage response to govern neural stem cell fate. Nature communications. 2023; 14(1):444. PM ID: 36707536
  • Shinmura, K, et al. (2023) Primary Cilia Are Frequently Present in Small Cell Lung Carcinomas but Not in Non-Small Cell Lung Carcinomas or Lung Carcinoids. Laboratory Investigation. 2023; 103(2):100007. Link: Laboratory Investigation
  • Griffin, TA, et al. (2023) Fibril treatment changes protein interactions of tau and α-synuclein in human neurons. The Journal of biological chemistry. 2023;:102888. PM ID: 36634849
  • Underhill, E & Toettcher, J. (2023) Control of gastruloid patterning and morphogenesis by the Erk and Akt signaling pathways. bioRxiv. 2023;. Link: bioRxiv
  • Takahashi, Y, et al. (2023) Transgenerational inheritance of acquired epigenetic signatures at CpG islands in mice. Cell. 2023; 186(4):715-731.e19. PM ID: 36754048
  • Tafessu, A, et al. (2023) H3.3 contributes to chromatin accessibility and transcription factor binding at promoter-proximal regulatory elements in embryonic stem cells. Genome biology. 2023; 24(1):25. PM ID: 36782260
  • Salmon, CK, et al. (2023) Organizing principles of astrocytic nanoarchitecture in the mouse cerebral cortex. Current biology : CB. 2023;. PM ID: 36805126
  • Loo, L, et al. (2023) Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional programs. PLoS biology. 2023; 21(2):e3001967. PM ID: 36757924
  • Mossine, V, et al. (2023) Screening a small hydrazide-hydrazone combinatorial library for targeting the STAT3 in monocyte-macrophages with insulated reporter transposons. Medicinal Chemistry Research. 2023;. Link: Medicinal Chemistry Research
  • Chang, J & Parent, LJ. (2023) HIV-1 Gag colocalizes with euchromatin histone marks at the nuclear periphery. bioRxiv : the preprint server for biology. 2023;. PM ID: 36865288
  • Vylegzhanina, A, et al. (2023) Cancer relevance of circulating antibodies against LINE-1 antigens in humans. bioRxiv. 2023;. Link: bioRxiv
  • Cosper, PF, et al. (2023) HPV16 E6 induces chromosomal instability due to polar chromosomes caused by E6AP-dependent degradation of the mitotic kinesin CENP-E. Proceedings of the National Academy of Sciences of the United States of America. 2023; 120(14):e2216700120. PM ID: 36989302
  • Ebisudani, T, et al. (2023) Genotype-phenotype mapping of a patient-derived lung cancer organoid biobank identifies NKX2-1-defined Wnt dependency in lung adenocarcinoma. Cell reports. 2023; 42(3):112212. PM ID: 36870059
  • Huang, M, et al. (2023) Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function. eLife. 2023; 12. PM ID: 36876906
  • Sinha, S, et al. (2023) A Multiomic Analysis Reveals How Breast Cancers Disseminated to the Bone Marrow Acquire Aggressive Phenotypes through Tumor-Stroma Tunnels. bioRxiv : the preprint server for biology. 2023;. PM ID: 36993616
  • Zhang, H, et al. (2023) Self-delivering CRISPR RNAs for AAV Co-delivery and Genome Editing in vivo. bioRxiv : the preprint server for biology. 2023;. PM ID: 36993169
PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector $1,061.00

Products

Catalog Number Description Size Price Quantity Add to Cart
PBQM812A-1 PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector 10 µg $1061
- +

Overview

Overview

Robust, titratable gene expression delivered using the PiggyBac Transposon System

More than just easy, consistent transgenesis with PiggyBac, the PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector (Cat.# PBQM812A-1) adds in the robust and titratable gene expression control of SBI’s cumate-inducible expression system. Clone your gene-of-interest into the MCS for cumate-inducible expression, which you can quantitatively monitor with the co-expressed GFP. This vector also co-expresses the cumate repressor, CymR, and puromycin resistance from the EF1α promoter. PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector With the PiggyBac Transposon System, you can:

  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
  • Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)

Customer Agreements

Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to evaluate the technology.

For end user license information, see the following:

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.

How It Works

How It Works

The PiggyBac Transposon System’s Cut-and-Paste Mechanism

The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.

The PiggyBac Transposon System’s cut-and-paste mechanism

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.

  • The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
  • The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
  • The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal

Tightly-controlled, inducible gene expression

Get robust, titratable gene expression with low background using SBI’s cumate-inducible vectors. These vectors take advantage of CymR, a repressor that binds to cumate operator sequences (CuO) with high affinity in the absence of cumate, a non-toxic small molecule. Providing much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.How the cumate operator switch works

  • Robust—increase expression up to 32-fold
  • Adjustable—tune expression levels by titrating the amount of cumate
  • Reversible—turn expression on, then off, then on again
  • Powerful—suitable for in vivo applications

Supporting Data

Supporting Data

Tight expression control with low background

In the absence of cumate, the cumate-inducible PiggyBac Vector shows undetectable levels of expression

Figure 2. In the absence of cumate, the cumate-inducible PiggyBac Vector shows undetectable levels of expression.

The PiggyBac cumate switch is titratable and can be turned off

Figure 3. The PiggyBac cumate switch is titratable and can be turned off.

FAQs

Citations

  • Brouwer, I, de Kort, MAC & Lenstra, TL. (2024) Measuring Transcription Dynamics of Individual Genes Inside Living Cells. Methods in molecular biology (Clifton, N.J.). 2024; 2694:235-265. PM ID: 37824008
  • Matta, SK, et al. (2024) Genome-wide and targeted CRISPR screens identify RNF213 as a mediator of interferon gamma-dependent pathogen restriction in human cells. Proceedings of the National Academy of Sciences of the United States of America. 2024; 121(1):e2315865120. PM ID: 38147552
  • Hurt, RC, et al. (2023) Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria. Nature biotechnology. 2023;. PM ID: 36593411
  • Yamazaki, K, et al. (2023) Multivalent mannose-conjugated siRNA causes robust gene silencing in pancreatic macrophages in vivo. European Journal of Pharmaceutics and Biopharmaceutics. 2023;. Link: European Journal of Pharmaceutics and Biopharmaceutics
  • Shahin, WS, et al. (2023) Redox-dependent Igfbp2 signaling controls Brca1 DNA damage response to govern neural stem cell fate. Nature communications. 2023; 14(1):444. PM ID: 36707536
  • Shinmura, K, et al. (2023) Primary Cilia Are Frequently Present in Small Cell Lung Carcinomas but Not in Non-Small Cell Lung Carcinomas or Lung Carcinoids. Laboratory Investigation. 2023; 103(2):100007. Link: Laboratory Investigation
  • Griffin, TA, et al. (2023) Fibril treatment changes protein interactions of tau and α-synuclein in human neurons. The Journal of biological chemistry. 2023;:102888. PM ID: 36634849
  • Underhill, E & Toettcher, J. (2023) Control of gastruloid patterning and morphogenesis by the Erk and Akt signaling pathways. bioRxiv. 2023;. Link: bioRxiv
  • Takahashi, Y, et al. (2023) Transgenerational inheritance of acquired epigenetic signatures at CpG islands in mice. Cell. 2023; 186(4):715-731.e19. PM ID: 36754048
  • Tafessu, A, et al. (2023) H3.3 contributes to chromatin accessibility and transcription factor binding at promoter-proximal regulatory elements in embryonic stem cells. Genome biology. 2023; 24(1):25. PM ID: 36782260
  • Salmon, CK, et al. (2023) Organizing principles of astrocytic nanoarchitecture in the mouse cerebral cortex. Current biology : CB. 2023;. PM ID: 36805126
  • Loo, L, et al. (2023) Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional programs. PLoS biology. 2023; 21(2):e3001967. PM ID: 36757924
  • Mossine, V, et al. (2023) Screening a small hydrazide-hydrazone combinatorial library for targeting the STAT3 in monocyte-macrophages with insulated reporter transposons. Medicinal Chemistry Research. 2023;. Link: Medicinal Chemistry Research
  • Chang, J & Parent, LJ. (2023) HIV-1 Gag colocalizes with euchromatin histone marks at the nuclear periphery. bioRxiv : the preprint server for biology. 2023;. PM ID: 36865288
  • Vylegzhanina, A, et al. (2023) Cancer relevance of circulating antibodies against LINE-1 antigens in humans. bioRxiv. 2023;. Link: bioRxiv
  • Cosper, PF, et al. (2023) HPV16 E6 induces chromosomal instability due to polar chromosomes caused by E6AP-dependent degradation of the mitotic kinesin CENP-E. Proceedings of the National Academy of Sciences of the United States of America. 2023; 120(14):e2216700120. PM ID: 36989302
  • Ebisudani, T, et al. (2023) Genotype-phenotype mapping of a patient-derived lung cancer organoid biobank identifies NKX2-1-defined Wnt dependency in lung adenocarcinoma. Cell reports. 2023; 42(3):112212. PM ID: 36870059
  • Huang, M, et al. (2023) Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function. eLife. 2023; 12. PM ID: 36876906
  • Sinha, S, et al. (2023) A Multiomic Analysis Reveals How Breast Cancers Disseminated to the Bone Marrow Acquire Aggressive Phenotypes through Tumor-Stroma Tunnels. bioRxiv : the preprint server for biology. 2023;. PM ID: 36993616
  • Zhang, H, et al. (2023) Self-delivering CRISPR RNAs for AAV Co-delivery and Genome Editing in vivo. bioRxiv : the preprint server for biology. 2023;. PM ID: 36993169