pAAVK-EF1α-MCS AAVanced™ Cloning and Expression Vector

Take advantage of AAV-based gene delivery for gene therapy development and other applications with this single promoter vector, pAAVK-EF1α-MCS.
  • Optimized for high titer rAAV production
  • Available in single and dual promoter formats
  • Compatible with any AAV packaging system
  • Designed for easy & efficient cloning
  • Choose fluorescent or antibiotic selection markers

Products

Catalog Number Description Size Price Quantity Add to Cart
AAV502A-1 pAAVK-EF1α -MCS AAVanced Cloning and Expression Vector 10 µg $589.00
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Contact Us

Overview

Overview

Harness AAV technology with SBI’s AAVanced rAAV Vectors

Widely used for gene therapy development and gene editing in vivo because of their broad tropism, lack of associated disease, the ability to transduce both dividing and non-dividing cells, and long-term transgene expression, recombinant AAV vectors are becoming increasingly popular. To help researchers take advantage of the powerful AAV system, SBI has developed a series of AAV vectors optimized for easy cloning and high titers. The pAAVK-EF1α-MCS AAVanced™ Cloning and Expression Vector is a streamlined AAV vector that enables the expression of a gene-of-interest by the EF1α promoter.

Because packaging into the AAV capsid limits the size of AAV vectors, the total amount of DNA between the two ITRs in SBI’s AAVanced Vectors needs to be 5 kb or less. For this reason, the streamlined pAAVK-EF1α-MCS vector is the best choice for researchers who need to insert larger amounts of DNA sequence.

pAAVK-EF1α -MCS AAVanced Cloning and Expression VectorChoose the right AAVanced Vector for your project SBI’s family of AAVanced Cloning and Expression Vectors support a range of projects:
  • Optimized for high titer rAAV production
  • Available in single and dual promoter formats
  • Compatible with any AAV packaging system
  • Designed for easy & efficient cloning
  • Choose fluorescent or antibiotic selection markers
Cat.#VectorMarkerFeatures
AAV502A-1pAAVK-EF1α-MCSNoneStreamlined size
AAV503A-1pAAVK-EF1α-MCS1-CMV-MCS2NoneFlexible—dual promoter, dual MCS
AAV526A-1pAAVK-EF1α-MCS-T2A-EGFPEGFPCo-expression with marker
AAV527A-1pAAVK-EF1α-MCS-T2A-PuroPuroCo-expression with marker
AAV528A-1pAAVK-EF1α–MCS-T2A-mRFPmRFPCo-expression with marker
AAV536A-1pAAVK-EF1α-MCS1-CMV-EGFPEGFPDual promoter with marker
AAV537A-1pAAVK-EF1α-MCS1-CMV-PuroPuroDual promoter with marker
AAV538A-1pAAVK-EF1α-MCS1-CMV-mRFPmRFPDual promoter with marker

How It Works

Supporting Data

Supporting Data

Effective gene delivery and expression with AAVanced Cloning and Expression Vectors

Effective gene delivery and expression with AAVanced Cloning and Expression Vectors

Figure 1. SBI’s single promoter AAVanced Cloning and Expression vectors are delivered effectively to target cells and express the desired markers. Representative images for GFP/RFP/Puro marker expression in HT1080 cells transduced with pAAVK-EF1α-MCS-T2A-EGFP (top row), pAAVK-EF1α-MCS-T2A-mRFP (middle row), or pAAVK-EF1α-MCS-T2A-Puro (bottom row). The top two rows compare cells transduced with the indicated vector packaged into AAV particles versus cells transfected with the same vector. Both conditions show good expression of GFP or RFP. The bottom row shows the number of cells present after three days of puromycin selection in the well with or without infection with pAAVK-EF1-MCS-2A-Puro packaged into AAV particles.

Resources

Citations

Products

Catalog Number Description Size Price Quantity Add to Cart
AAV502A-1 pAAVK-EF1α -MCS AAVanced Cloning and Expression Vector 10 µg $589.00
- +
Contact Us

Overview

Overview

Harness AAV technology with SBI’s AAVanced rAAV Vectors

Widely used for gene therapy development and gene editing in vivo because of their broad tropism, lack of associated disease, the ability to transduce both dividing and non-dividing cells, and long-term transgene expression, recombinant AAV vectors are becoming increasingly popular. To help researchers take advantage of the powerful AAV system, SBI has developed a series of AAV vectors optimized for easy cloning and high titers. The pAAVK-EF1α-MCS AAVanced™ Cloning and Expression Vector is a streamlined AAV vector that enables the expression of a gene-of-interest by the EF1α promoter.

Because packaging into the AAV capsid limits the size of AAV vectors, the total amount of DNA between the two ITRs in SBI’s AAVanced Vectors needs to be 5 kb or less. For this reason, the streamlined pAAVK-EF1α-MCS vector is the best choice for researchers who need to insert larger amounts of DNA sequence.

pAAVK-EF1α -MCS AAVanced Cloning and Expression VectorChoose the right AAVanced Vector for your project SBI’s family of AAVanced Cloning and Expression Vectors support a range of projects:
  • Optimized for high titer rAAV production
  • Available in single and dual promoter formats
  • Compatible with any AAV packaging system
  • Designed for easy & efficient cloning
  • Choose fluorescent or antibiotic selection markers
Cat.#VectorMarkerFeatures
AAV502A-1pAAVK-EF1α-MCSNoneStreamlined size
AAV503A-1pAAVK-EF1α-MCS1-CMV-MCS2NoneFlexible—dual promoter, dual MCS
AAV526A-1pAAVK-EF1α-MCS-T2A-EGFPEGFPCo-expression with marker
AAV527A-1pAAVK-EF1α-MCS-T2A-PuroPuroCo-expression with marker
AAV528A-1pAAVK-EF1α–MCS-T2A-mRFPmRFPCo-expression with marker
AAV536A-1pAAVK-EF1α-MCS1-CMV-EGFPEGFPDual promoter with marker
AAV537A-1pAAVK-EF1α-MCS1-CMV-PuroPuroDual promoter with marker
AAV538A-1pAAVK-EF1α-MCS1-CMV-mRFPmRFPDual promoter with marker

How It Works

Supporting Data

Supporting Data

Effective gene delivery and expression with AAVanced Cloning and Expression Vectors

Effective gene delivery and expression with AAVanced Cloning and Expression Vectors

Figure 1. SBI’s single promoter AAVanced Cloning and Expression vectors are delivered effectively to target cells and express the desired markers. Representative images for GFP/RFP/Puro marker expression in HT1080 cells transduced with pAAVK-EF1α-MCS-T2A-EGFP (top row), pAAVK-EF1α-MCS-T2A-mRFP (middle row), or pAAVK-EF1α-MCS-T2A-Puro (bottom row). The top two rows compare cells transduced with the indicated vector packaged into AAV particles versus cells transfected with the same vector. Both conditions show good expression of GFP or RFP. The bottom row shows the number of cells present after three days of puromycin selection in the well with or without infection with pAAVK-EF1-MCS-2A-Puro packaged into AAV particles.

Citations