Oct4 CR4 pGreenFire (EF1α-puro) Response Reporter

Verify pluripotency and measure Oct4 expression using a pGreenFire (GFP-luciferase) reporter driven by the Oct4 conserved enhancer CR4 – includes EF1α-puro
  • Create stable cell lines for quantitation of Oct4 expression
  • Track differentiation in live cells in real time

Products

Catalog Number Description Size Price Quantity Add to Cart
SR20070-PA-P Oct4 CR4-pGreenFire Response Reporter (with EF1α-Puro) 10 µg $1,594.00
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Contact Us

Overview

Overview

Monitor and measure the expression of pluripotency markers With SBI’s line of pGreenFire Pluripotency Response Reporter Vectors, you can monitor and measure the expression of pluripotency markers in live cells in real time. These vectors leverage our reliable lentivector technology and save you time—the pre-built reporters come as ready-to-package lentivector plasmids, with some reporters also available as ready-to-transduce, pre-packaged virus. All of our Response Reporters deliver great performance in both human and mouse cells. The Oct4 CR4 pGreenFire (EF1α-puro) Response Reporter uses the Oct4 CR4 enhancer with a minimal CMV promoter to co-express GFP and luciferase. Co-expression is mediated by a T2 element. The minimal CMV promoter alone delivers negligible expression, but when downstream of CR4 enhancer elements, drives expression of dscGFP and luciferase under conditions where Oct4 is expressed. The result is the ability to quantitatively measure Oct4 expression by fluorescence and luciferase activity. The vector also includes a constitutively-expressed puromycin marker (EF1α promoter) to simplify cell line construction. Or choose a constitutively expressed neomycin marker (Cat.# SR20070-PA-N), or no marker at all (Cat.# SR20070-PA-1).
  • Create stable cell lines for quantitation of Oct4 expression
  • Track differentiation in live cells in real time
Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes. Choose the right vector to monitor pluripotency markers (Pluripotency Reporters) or to measure the activity of pluripotency makers (Pluripotency Response Reporters) Pluripotency response reporters
SBI’s Pluripotency Response Reporters work for both human and mouse stem cells   
Catalog #Promoter/Enhancer elementsReporterSelection cassette
SR20070-PA/VA-1Oct4 CR4pGreenFire (GFP-Luc)None
SR20070-PA-NOct4 CR4pGreenFire (GFP-Luc)EF1α-Neo
SR20070-PA-POct4 CR4pGreenFire (GFP-Luc)EF1α-Puro
SR20071-PA/VA-1Sox2 SRR2pGreenFire (GFP-Luc)None
SR20071-PA-NSox2 SRR2pGreenFire (GFP-Luc)EF1α-Neo
SR20071-PA/VA-PSox2 SRR2pGreenFire (GFP-Luc)EF1α-Puro
Pluripotency reporters
Catalog #SpeciesPromoter/enhancer elementReporter
SR10033PA/VA-1HumanOct-4pGreenZeo
SR10043PA/VA-1HumanOct-4pRedZeo
SR10053PA/VA-1HumanOct-4pRedTK
SR10029PA/VA-1MouseOct-4pGreenZeo
SR10045PA/VA-1MouseOct-4pRedZeo
SR10054PA/VA-1MouseOct-4pRedTK
SR10030PA/VA-1HumanNanogpGreenZeo
SR10042PA/VA-1HumanNanogpRedZeo
SR10055PA/VA-1HumanNanogpRedTK
SR10031PA/VA-1MouseNanogpGreenZeo
SR10044PA/VA-1MouseNanogpRedZeo
SR10056PA/VA-1MouseNanogpRedTK

How It Works

Supporting Data

Supporting Data

See our pluripotency response reporters in action Track and measure the activity of pluripotency markers in live cells

Resources

Citations

Products

Catalog Number Description Size Price Quantity Add to Cart
SR20070-PA-P Oct4 CR4-pGreenFire Response Reporter (with EF1α-Puro) 10 µg $1,594.00
- +
Contact Us

Overview

Overview

Monitor and measure the expression of pluripotency markers With SBI’s line of pGreenFire Pluripotency Response Reporter Vectors, you can monitor and measure the expression of pluripotency markers in live cells in real time. These vectors leverage our reliable lentivector technology and save you time—the pre-built reporters come as ready-to-package lentivector plasmids, with some reporters also available as ready-to-transduce, pre-packaged virus. All of our Response Reporters deliver great performance in both human and mouse cells. The Oct4 CR4 pGreenFire (EF1α-puro) Response Reporter uses the Oct4 CR4 enhancer with a minimal CMV promoter to co-express GFP and luciferase. Co-expression is mediated by a T2 element. The minimal CMV promoter alone delivers negligible expression, but when downstream of CR4 enhancer elements, drives expression of dscGFP and luciferase under conditions where Oct4 is expressed. The result is the ability to quantitatively measure Oct4 expression by fluorescence and luciferase activity. The vector also includes a constitutively-expressed puromycin marker (EF1α promoter) to simplify cell line construction. Or choose a constitutively expressed neomycin marker (Cat.# SR20070-PA-N), or no marker at all (Cat.# SR20070-PA-1).
  • Create stable cell lines for quantitation of Oct4 expression
  • Track differentiation in live cells in real time
Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes. Choose the right vector to monitor pluripotency markers (Pluripotency Reporters) or to measure the activity of pluripotency makers (Pluripotency Response Reporters) Pluripotency response reporters
SBI’s Pluripotency Response Reporters work for both human and mouse stem cells   
Catalog #Promoter/Enhancer elementsReporterSelection cassette
SR20070-PA/VA-1Oct4 CR4pGreenFire (GFP-Luc)None
SR20070-PA-NOct4 CR4pGreenFire (GFP-Luc)EF1α-Neo
SR20070-PA-POct4 CR4pGreenFire (GFP-Luc)EF1α-Puro
SR20071-PA/VA-1Sox2 SRR2pGreenFire (GFP-Luc)None
SR20071-PA-NSox2 SRR2pGreenFire (GFP-Luc)EF1α-Neo
SR20071-PA/VA-PSox2 SRR2pGreenFire (GFP-Luc)EF1α-Puro
Pluripotency reporters
Catalog #SpeciesPromoter/enhancer elementReporter
SR10033PA/VA-1HumanOct-4pGreenZeo
SR10043PA/VA-1HumanOct-4pRedZeo
SR10053PA/VA-1HumanOct-4pRedTK
SR10029PA/VA-1MouseOct-4pGreenZeo
SR10045PA/VA-1MouseOct-4pRedZeo
SR10054PA/VA-1MouseOct-4pRedTK
SR10030PA/VA-1HumanNanogpGreenZeo
SR10042PA/VA-1HumanNanogpRedZeo
SR10055PA/VA-1HumanNanogpRedTK
SR10031PA/VA-1MouseNanogpGreenZeo
SR10044PA/VA-1MouseNanogpRedZeo
SR10056PA/VA-1MouseNanogpRedTK

How It Works

Supporting Data

Supporting Data

See our pluripotency response reporters in action Track and measure the activity of pluripotency markers in live cells

Citations