NF-κB/293/GFP-Luc™ Transcriptional Reporter Cell Line
Monitor NF-κB signaling in vitro and screen for genetic and/or small molecule inhibitors and activators, and more – sort with GFP and quantify with luciferase
- Study NF-κB signaling in 293 TN cells
- Perform live cell imaging or FACS (300-fold over background NF-κB-dependent GFP signal)
- Measure pathway activity with luciferase
- Compatible with a variety of screening methods including small molecule and RNAi
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| TR860A-1 | NF-kB/293/GFP-Luc Transcriptional Reporter Cell Line | >2 x 10^6 Cells | $3142 |
|
||||
Overview
Overview
Monitor NF-κB signaling in Jurkat cells Speed your studies with this pre-built 293 TN-based cell line for monitoring NF-κB signaling in real time. We’ve already integrated an expression cassette that includes NF-κB-responsive transcriptional elements upstream of a minimal CMV promoter (mCMV)-GFP-luciferase cassette. Expression of GFP (up to 300-fold over background) and luciferase only occurs in the presence of active NF-κB signaling, enabling screening for genetic and/or small molecule inhibitors and activators of the NF-κB signaling pathway. Use GFP fluorescence for live cell imaging and FACS, or luciferase activity to quantitatively measure activity of the signaling pathway.- Study NF-κB signaling in 293 TN cells
- Perform live cell imaging or FACS (300-fold over background NF-κB-dependent GFP signal)
- Measure pathway activity with luciferase
- Compatible with a variety of screening methods including small molecule and RNAi
How It Works
Supporting Data
Supporting Data
See the NF-κB/293/GFP-luc Transcriptional Reporter Cell Line in action
Monitor NF-κB in real time with GFP
Quantitate NF-κB Activation with luciferase assays
Resources
Citations
-
Ishino, T, et al. (2023) Somatic mutations can induce a noninflamed tumour microenvironment via their original gene functions, despite deriving neoantigens. British journal of cancer. 2023;. PM ID: 36732592
-
Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 2022;. PM ID: 35483375
-
Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 2022;. Link: slas-discovery.org
-
Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 2022; 21(6):710-720. PM ID: 35606429
-
Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 2022;. Link: Research Square
-
Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 2022; 11(7). PM ID: 35884187
-
Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 2022; 25:101508. PM ID: 35985204
-
Chen, C, et al. (2022) ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Nature communications. 2022; 13(1):6108. PM ID: 36245009
-
Takase, S, et al. (2022) 17β-neriifolin from unripe fruits of Cerbera manghas suppressed cell proliferation via the inhibition of HOXA9-dependent transcription and the induction of apoptosis in the human AML cell line THP-1. Journal of natural medicines. 2022;. PM ID: 36266527
-
Donohue, L, et al. (2022) A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation. Cell Genomics. 2022;:100191. Link: Cell Genomics
-
Caligiuri, SPB, et al. (2022) Hedgehog-interacting protein acts in the habenula to regulate nicotine intake. Proceedings of the National Academy of Sciences of the United States of America. 2022; 119(46):e2209870119. PM ID: 36346845
-
Tan, TG, et al. (2022) SPATA2 and CYLD inhibit T cell infiltration into colorectal cancer via regulation of IFN-γ/STAT1 axis. Frontiers in oncology. 2022; 12:1016307. PM ID: 36531014
-
Mauro-Lizcano, M, Sotgia, F & Lisanti, MP. (2022) SOX2-high cancer cells exhibit an aggressive phenotype, with increases in stemness, proliferation and invasion, as well as higher metabolic activity and ATP production. Aging. 2022; 14(24):9877-9889. PM ID: 36566021
-
Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 2021;. PM ID: 33545065
-
Deng, P, et al. (2021) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell stem cell. 2021;. PM ID: 33571444
-
Bencomo-Alvarez, AE, et al. (2021) Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B. Oncogene. 2021;. PM ID: 33712704
-
Miyashita, Y, et al. (2021) The CR9 element is a novel mechanical load-responsive enhancer that regulates natriuretic peptide genes expression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2021; 35(4):e21495. PM ID: 33689182
-
Sugali, CK, et al. (2021) Full title: The canonical Wnt signaling pathway inhibits the glucocorticoid receptor signaling pathway in the trabecular meshwork. The American journal of pathology. 2021;. PM ID: 33705750
-
Cook, B, et al. (2021) The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3 β signaling. The Journal of biological chemistry. 2021;:100696. PM ID: 33895139
-
Slominski, AT, et al. (2021) Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs). Scientific reports. 2021; 11(1):8002. PM ID: 33850196
- See More
Related Products
NF-κB/293/GFP-Luc™ Transcriptional Reporter Cell Line
$3,142.00
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| TR860A-1 | NF-kB/293/GFP-Luc Transcriptional Reporter Cell Line | >2 x 10^6 Cells | $3142 |
|
||||
Overview
Overview
Monitor NF-κB signaling in Jurkat cells Speed your studies with this pre-built 293 TN-based cell line for monitoring NF-κB signaling in real time. We’ve already integrated an expression cassette that includes NF-κB-responsive transcriptional elements upstream of a minimal CMV promoter (mCMV)-GFP-luciferase cassette. Expression of GFP (up to 300-fold over background) and luciferase only occurs in the presence of active NF-κB signaling, enabling screening for genetic and/or small molecule inhibitors and activators of the NF-κB signaling pathway. Use GFP fluorescence for live cell imaging and FACS, or luciferase activity to quantitatively measure activity of the signaling pathway.- Study NF-κB signaling in 293 TN cells
- Perform live cell imaging or FACS (300-fold over background NF-κB-dependent GFP signal)
- Measure pathway activity with luciferase
- Compatible with a variety of screening methods including small molecule and RNAi
How It Works
Supporting Data
Supporting Data
See the NF-κB/293/GFP-luc Transcriptional Reporter Cell Line in action
Monitor NF-κB in real time with GFP
Quantitate NF-κB Activation with luciferase assays
Citations
-
Ishino, T, et al. (2023) Somatic mutations can induce a noninflamed tumour microenvironment via their original gene functions, despite deriving neoantigens. British journal of cancer. 2023;. PM ID: 36732592
-
Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 2022;. PM ID: 35483375
-
Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 2022;. Link: slas-discovery.org
-
Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 2022; 21(6):710-720. PM ID: 35606429
-
Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 2022;. Link: Research Square
-
Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 2022; 11(7). PM ID: 35884187
-
Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 2022; 25:101508. PM ID: 35985204
-
Chen, C, et al. (2022) ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Nature communications. 2022; 13(1):6108. PM ID: 36245009
-
Takase, S, et al. (2022) 17β-neriifolin from unripe fruits of Cerbera manghas suppressed cell proliferation via the inhibition of HOXA9-dependent transcription and the induction of apoptosis in the human AML cell line THP-1. Journal of natural medicines. 2022;. PM ID: 36266527
-
Donohue, L, et al. (2022) A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation. Cell Genomics. 2022;:100191. Link: Cell Genomics
-
Caligiuri, SPB, et al. (2022) Hedgehog-interacting protein acts in the habenula to regulate nicotine intake. Proceedings of the National Academy of Sciences of the United States of America. 2022; 119(46):e2209870119. PM ID: 36346845
-
Tan, TG, et al. (2022) SPATA2 and CYLD inhibit T cell infiltration into colorectal cancer via regulation of IFN-γ/STAT1 axis. Frontiers in oncology. 2022; 12:1016307. PM ID: 36531014
-
Mauro-Lizcano, M, Sotgia, F & Lisanti, MP. (2022) SOX2-high cancer cells exhibit an aggressive phenotype, with increases in stemness, proliferation and invasion, as well as higher metabolic activity and ATP production. Aging. 2022; 14(24):9877-9889. PM ID: 36566021
-
Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 2021;. PM ID: 33545065
-
Deng, P, et al. (2021) Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell stem cell. 2021;. PM ID: 33571444
-
Bencomo-Alvarez, AE, et al. (2021) Proteasome 26S subunit, non-ATPases 1 (PSMD1) and 3 (PSMD3), play an oncogenic role in chronic myeloid leukemia by stabilizing nuclear factor-kappa B. Oncogene. 2021;. PM ID: 33712704
-
Miyashita, Y, et al. (2021) The CR9 element is a novel mechanical load-responsive enhancer that regulates natriuretic peptide genes expression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2021; 35(4):e21495. PM ID: 33689182
-
Sugali, CK, et al. (2021) Full title: The canonical Wnt signaling pathway inhibits the glucocorticoid receptor signaling pathway in the trabecular meshwork. The American journal of pathology. 2021;. PM ID: 33705750
-
Cook, B, et al. (2021) The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3 β signaling. The Journal of biological chemistry. 2021;:100696. PM ID: 33895139
-
Slominski, AT, et al. (2021) Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs). Scientific reports. 2021; 11(1):8002. PM ID: 33850196
- See More