mmu-miRNome MicroRNA Profiling Kit (Mouse)

Streamline your studies of mouse miRNAs with this qPCR profiling kit that includes primers for over 700 miRNAs plus SBI’s sensitive QuantiMir Kit

Products

Catalog Number Description Size Price Quantity Add to Cart
RA670A-1 mmu-miRNome MicroRNA Profiling Kit (Mouse) 20 Profiles $1,552.00
- +
Contact Us

Overview

Overview

Profile across the mouse miRNome SBI has simplified qPCR profiling of mouse miRNAs by bringing together a comprehensive qPCR array of mouse miRNAs. Our Mouse mmu-miRNome MicroRNA Profiling Kit comes with all the reagents necessary to tag and convert small RNAs into quantifiable cDNA using the sensitive QuantiMir™ technology and a large set of mouse miRNA assays, including major and minor miR forms (709 mature miRNAs). The kits include assays in two, pre-formatted 384-well assay plates with three endogenous reference miRNA controls on each plate (U1, U6, and RNU43). All microRNA assays are based on miRBase entries.
  • Identify miRNA biomarkers and expression pattern signatures
  • Rapidly tag and convert all small RNAs into detectable cDNA for qPCR
  • Measure as little as picogram amounts of starting total RNA
  • Conduct high-throughput screens of cell lines, tissues, and clinical samples
The most comprehensive qPCR arrays available only from SBIAbout the included QuantiMir Kit When you’d like to profile miRNAs with qPCR, SBI’s QuantiMir™ Kit can get your samples ready. By efficiently converting all of your miRNA into cDNA, you can get accurate, sensitive, and quantitative qPCR data on the miRs in your sample. Great for understanding differential miRNA expression, simultaneously profiling siRNA and mRNA, and more.
  • Simple and robust procedure
  • Rapidly and efficiently convert all small RNAs into cDNAs for qPCR
  • Suitable for high-throughput screening of clinical samples
  • Sensitive and accurate
  • Versatile—design your own miRNA assays

How It Works

How It Works

Using the mmu-miRNome MicroRNA Profiling Kit

First, prepare RNA for qPCR using the QuantiMir Kit

Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.

  • Tag all small RNAs with a poly-A tail
  • Anneal an oligo-dT adaptor
  • Reverse transcribe to create first-strand cDNA

The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.

Then conduct qPCR assays

Get additional information about the array including miRNA sequences, miRbase accession numbers, and qPCR analysis templates in the Excel document, “Free Analysis Software for Mouse miRNome Ver. 14

Supporting Data

Supporting Data

miRNome Profiler Performance Data

Accurately normalize your microRNA expression profiling data using three reference controls—U6 snRNA, RNU1A, and RNU43

Figure 1. Accurately normalize your microRNA expression profiling data using three reference controls—U6 snRNA, RNU1A, and RNU43.

Get high reproducibility—excellent technical replicates across 8-logs

Figure 2. Get high reproducibility—excellent technical replicates across 8-logs. Reliable replicates from plate-to-plate enable precise comparisons of numerous samples across large microRNA profiling studies.

Resources

Citations

We're sorry, an error has occurred while generating this content.

Products

Catalog Number Description Size Price Quantity Add to Cart
RA670A-1 mmu-miRNome MicroRNA Profiling Kit (Mouse) 20 Profiles $1,552.00
- +
Contact Us

Overview

Overview

Profile across the mouse miRNome SBI has simplified qPCR profiling of mouse miRNAs by bringing together a comprehensive qPCR array of mouse miRNAs. Our Mouse mmu-miRNome MicroRNA Profiling Kit comes with all the reagents necessary to tag and convert small RNAs into quantifiable cDNA using the sensitive QuantiMir™ technology and a large set of mouse miRNA assays, including major and minor miR forms (709 mature miRNAs). The kits include assays in two, pre-formatted 384-well assay plates with three endogenous reference miRNA controls on each plate (U1, U6, and RNU43). All microRNA assays are based on miRBase entries.
  • Identify miRNA biomarkers and expression pattern signatures
  • Rapidly tag and convert all small RNAs into detectable cDNA for qPCR
  • Measure as little as picogram amounts of starting total RNA
  • Conduct high-throughput screens of cell lines, tissues, and clinical samples
The most comprehensive qPCR arrays available only from SBIAbout the included QuantiMir Kit When you’d like to profile miRNAs with qPCR, SBI’s QuantiMir™ Kit can get your samples ready. By efficiently converting all of your miRNA into cDNA, you can get accurate, sensitive, and quantitative qPCR data on the miRs in your sample. Great for understanding differential miRNA expression, simultaneously profiling siRNA and mRNA, and more.
  • Simple and robust procedure
  • Rapidly and efficiently convert all small RNAs into cDNAs for qPCR
  • Suitable for high-throughput screening of clinical samples
  • Sensitive and accurate
  • Versatile—design your own miRNA assays

How It Works

How It Works

Using the mmu-miRNome MicroRNA Profiling Kit

First, prepare RNA for qPCR using the QuantiMir Kit

Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.

  • Tag all small RNAs with a poly-A tail
  • Anneal an oligo-dT adaptor
  • Reverse transcribe to create first-strand cDNA

The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.

Then conduct qPCR assays

Get additional information about the array including miRNA sequences, miRbase accession numbers, and qPCR analysis templates in the Excel document, “Free Analysis Software for Mouse miRNome Ver. 14

Supporting Data

Supporting Data

miRNome Profiler Performance Data

Accurately normalize your microRNA expression profiling data using three reference controls—U6 snRNA, RNU1A, and RNU43

Figure 1. Accurately normalize your microRNA expression profiling data using three reference controls—U6 snRNA, RNU1A, and RNU43.

Get high reproducibility—excellent technical replicates across 8-logs

Figure 2. Get high reproducibility—excellent technical replicates across 8-logs. Reliable replicates from plate-to-plate enable precise comparisons of numerous samples across large microRNA profiling studies.

Citations

We're sorry, an error has occurred while generating this content.